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  1. Article ; Online: Hemin competitively inhibits HSPA8 ATPase activity mitigating its foldase function.

    Pandey, Alok Kumar / Trivedi, Vishal

    Archives of biochemistry and biophysics

    2024  Volume 752, Page(s) 109889

    Abstract: Hemolysis in red blood cells followed by hemoglobin degradation results in high hemin levels in the systemic circulation. Such a level of hemin is disastrous for cells and tissues and is considerably responsible for the pathologies of diseases like ... ...

    Abstract Hemolysis in red blood cells followed by hemoglobin degradation results in high hemin levels in the systemic circulation. Such a level of hemin is disastrous for cells and tissues and is considerably responsible for the pathologies of diseases like severe malaria. Hemin's hydrophobic chemical nature and structure allow it to bind several proteins leading to their functional modification. Such modifications in physiologically relevant proteins can have a high impact on various cellular processes. HSPA8 is a chaperone that has a protective role in oxidative stress by aiding protein refolding. Through ATPase activity assays we found that hemin can competitively inhibit ATP hydrolysis by the chaperone HSPA8. Hemin as such does not affect the structural integrity of the protein which is inferred from CD spectroscopy and Gel filtration but it hinders the ATP-dependent foldase function of the chaperone. HSPA8 was not able to cause the refolding of the model protein lysozyme in the presence of hemin. The loss in HSPA8 function was due to competition between hemin and ATP as the chaperone was able to regain the foldase function when the concentration of ATP was gradually increased with hemin present at the inhibitory concentration. In-silico studies to establish the competition for the specific binding site revealed that ATP was unable to replace hemin from the ATP binding pocket of HSPA8 and was forced to form a non-specific and unstable complex. In-vitro isothermal calorimetry revealed that the affinity of ATP for binding to HSPA8 was reduced 22 folds in the presence of hemin. The prevention of HSPA8's cytoprotective function by hemin can be a major factor contributing to the overall cellular damage during hemin accumulation in the case of severe malaria and other hemolytic diseases.
    MeSH term(s) Humans ; Hemin/pharmacology ; Molecular Chaperones ; Hemolysis ; Malaria ; Adenosine Triphosphatases/metabolism ; Adenosine Triphosphate/metabolism ; HSC70 Heat-Shock Proteins
    Chemical Substances Hemin (743LRP9S7N) ; Molecular Chaperones ; Adenosine Triphosphatases (EC 3.6.1.-) ; Adenosine Triphosphate (8L70Q75FXE) ; HSPA8 protein, human ; HSC70 Heat-Shock Proteins
    Language English
    Publishing date 2024-01-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 523-x
    ISSN 1096-0384 ; 0003-9861
    ISSN (online) 1096-0384
    ISSN 0003-9861
    DOI 10.1016/j.abb.2024.109889
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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Role Transformation of HSPA8 to Heme-peroxidase After Binding Hemin to Catalyze Heme Polymerization.

    Pandey, Alok Kumar / Trivedi, Vishal

    The protein journal

    2023  Volume 43, Issue 1, Page(s) 48–61

    Abstract: Hemin, a byproduct of hemoglobin degradation, inflicts oxidative insult to cells. Following its accumulation, several proteins are recruited for heme detoxification with heme oxygenase playing the key role. Chaperones play a protective role primarily by ... ...

    Abstract Hemin, a byproduct of hemoglobin degradation, inflicts oxidative insult to cells. Following its accumulation, several proteins are recruited for heme detoxification with heme oxygenase playing the key role. Chaperones play a protective role primarily by preventing protein degradation and unfolding. They also are known to have miscellaneous secondary roles during similar situations. To discover a secondary role of chaperones during heme stress we studied the role of the chaperone HSPA8 in the detoxification of hemin. In-silico studies indicated that HSPA8 has a well-defined biophoric environment to bind hemin. Through optical difference spectroscopy, we found that HSPA8 binds hemin through its N-terminal domain with a K
    MeSH term(s) Hemin/chemistry ; Hemin/metabolism ; Heme/chemistry ; Hydrogen Peroxide ; Polymerization ; Peroxidases
    Chemical Substances Hemin (743LRP9S7N) ; Heme (42VZT0U6YR) ; Hydrogen Peroxide (BBX060AN9V) ; Peroxidases (EC 1.11.1.-)
    Language English
    Publishing date 2023-12-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2143071-8
    ISSN 1875-8355 ; 1572-3887
    ISSN (online) 1875-8355
    ISSN 1572-3887
    DOI 10.1007/s10930-023-10167-9
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  3. Book: The developing human

    Moore, Keith L. / Persaud, Trivedi V. N. / Torchia, Mark G.

    clinically oriented embryology

    2020  

    Author's details Keith L. Moore, T. V. N. (Vid) Persaud, Mark G. Torchia
    Keywords Embryologie
    Language English
    Size xiii, 503 Seiten, Illustrationen
    Edition Eleventh edition
    Publisher Elsevier
    Publishing place Edinburgh
    Publishing country Great Britain
    Document type Book
    Note Zugang zur Online-Ausgabe über Code
    Remark QS 604
    HBZ-ID HT020043522
    ISBN 978-0-323-61155-8 ; 978-0-323-61154-1 ; 9780323611565 ; 0-323-61155-9 ; 0-323-61154-0 ; 0323611567
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2019 A 597 available for loan (reading room) Lesesaal EG
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  4. Book: Before we are born

    Moore, Keith L. / Persaud, Trivedi V. N. / Torchia, Mark G.

    essentials of embryology and birth defects

    2020  

    Author's details Keith L. Moore, Professor Emeritus, T.V.N. Persaud, Professor Emeritus and Former Head, MD, Mark G. Torchia, Associate Professor
    Keywords Embryonic Development ; Congenital Abnormalities ; Embryology / methods ; Fetal Development ; Embryology, Human ; Abnormalities, Human
    Subject code 612.64
    Language English
    Size xiii, 334 Seiten, Illustrationen, 28 cm
    Edition Tenth edition
    Publisher Saunders
    Publishing place Edinburgh
    Publishing country Great Britain
    Document type Book
    Note Zugang zu zusätzlichem Internetmaterial über Code ; Includes bibliographical references and index
    HBZ-ID HT020381963
    ISBN 978-0-323-60849-7 ; 9780323608503 ; 0-323-60849-3 ; 0323608507
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2020 A 454 available for loan (reading room) Lesesaal EG
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  5. Article ; Online: Sculpting tissues by phase transitions.

    Lenne, Pierre-François / Trivedi, Vikas

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 664

    Abstract: Biological systems display a rich phenomenology of states that resemble the physical states of matter - solid, liquid and gas. These phases result from the interactions between the microscopic constituent components - the cells - that manifest in ... ...

    Abstract Biological systems display a rich phenomenology of states that resemble the physical states of matter - solid, liquid and gas. These phases result from the interactions between the microscopic constituent components - the cells - that manifest in macroscopic properties such as fluidity, rigidity and resistance to changes in shape and volume. Looked at from such a perspective, phase transitions from a rigid to a flowing state or vice versa define much of what happens in many biological processes especially during early development and diseases such as cancer. Additionally, collectively moving confluent cells can also lead to kinematic phase transitions in biological systems similar to multi-particle systems where the particles can interact and show sub-populations characterised by specific velocities. In this Perspective we discuss the similarities and limitations of the analogy between biological and inert physical systems both from theoretical perspective as well as experimental evidence in biological systems. In understanding such transitions, it is crucial to acknowledge that the macroscopic properties of biological materials and their modifications result from the complex interplay between the microscopic properties of cells including growth or death, neighbour interactions and secretion of matrix, phenomena unique to biological systems. Detecting phase transitions in vivo is technically difficult. We present emerging approaches that address this challenge and may guide our understanding of the organization and macroscopic behaviour of biological tissues.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Cell Adhesion/physiology ; Cell Communication/physiology ; Chemical Phenomena ; Computer Simulation ; Humans ; Intracellular Space/chemistry ; Models, Theoretical ; Phase Transition ; Thermodynamics ; Viscoelastic Substances/chemistry
    Chemical Substances Viscoelastic Substances
    Language English
    Publishing date 2022-02-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-28151-9
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  6. Article ; Online: High-Flow Nasal Cannula Compared With Noninvasive Positive Pressure Ventilation in Acute Hypoxic Respiratory Failure: A Systematic Review and Meta-Analysis.

    Chaudhuri, Dipayan / Trivedi, Vatsal / Lewis, Kimberley / Rochwerg, Bram

    Critical care explorations

    2023  Volume 5, Issue 4, Page(s) e0892

    Abstract: To evaluate the efficacy and cost-effectiveness of high-flow nasal cannula (HFNC) when compared with noninvasive positive pressure ventilation (NIPPV) in patients with acute hypoxic respiratory failure (AHRF).: Data sources: We performed a ... ...

    Abstract To evaluate the efficacy and cost-effectiveness of high-flow nasal cannula (HFNC) when compared with noninvasive positive pressure ventilation (NIPPV) in patients with acute hypoxic respiratory failure (AHRF).
    Data sources: We performed a comprehensive search of MEDLINE, Embase, CINAHL, the Cochrane library, and the international Health Technology Assessment database from inception to September 14, 2022.
    Study selection: We included randomized control studies that compared HFNC to NIPPV in adult patients with AHRF. For clinical outcomes, we included only parallel group and crossover randomized control trials (RCTs). For economic outcomes, we included any study design that evaluated cost-effectiveness, cost-utility, or cost benefit analyses.
    Data extraction: Clinical outcomes of interest included intubation, mortality, ICU and hospital length of stay (LOS), and patient-reported dyspnea. Economic outcomes of interest included costs, cost-effectiveness, and cost-utility.
    Data synthesis: We included nine RCTs (
    Conclusions: HFNC and NIPPV may be similarly effective at reducing the need for intubation with an uncertain effect on mortality in hospitalized patients with hypoxemic respiratory failure. More research evaluating different interfaces in varying clinical contexts is needed to improve generalizability and precision of findings.
    Language English
    Publishing date 2023-03-28
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2639-8028
    ISSN (online) 2639-8028
    DOI 10.1097/CCE.0000000000000892
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  7. Article ; Online: Extraction, phytochemical characterization and anti-cancer mechanism of Haritaki churna: An ayurvedic formulation.

    Khan, Md Rafi Uz Zama / Yanase, Emiko / Trivedi, Vishal

    PloS one

    2023  Volume 18, Issue 5, Page(s) e0286274

    Abstract: Haritaki churna (HC), a single herb ayurvedic formulations is known to be prescribed for various gastro-intestinal disorders in Ayurveda. Haritaki churna aqueous extract (HCAE) has anti-cancer activity against different types of cancer cells with an IC50 ...

    Abstract Haritaki churna (HC), a single herb ayurvedic formulations is known to be prescribed for various gastro-intestinal disorders in Ayurveda. Haritaki churna aqueous extract (HCAE) has anti-cancer activity against different types of cancer cells with an IC50 in the range of 50-97 μg/ml. Bioavailability of Haritaki Churna is very high in digestive track and treatment of colorectal cancer cells HCT-116, DLD1, HT-29 with HCAE reduces its cellular viability with anti-cancer IC50 70μg/ml. HCAE consumption is safe for human as it didn't affect the cellular viability of primary human PBMCs or non-cancerogenic HEK-293 cells. Haritaki churna was found to be stable in biological gastric fluids and bioactive agents are not losing their anti-cancer activity under such harsh conditions. The HPLC Chromatogram of HCAE is giving 13 major peaks and 11 minor peaks. Exploiting LC-MS, IR and NMR spectroscopic techniques, a total of 13 compounds were identified from HCAE namely Shikimic acid, Chebulic acid, gallic acid, 5-hydroxymethylfurfural, Protocatechuic acid, 4-O-galloyl-shikimic Acid, 5-O-galloyl-shikimic Acid, Methylgallate, corilagin, 1, 2, 6, Tri-O-galloyl β-D-glucose, chebulagic acid, chebulinic acid, and Ellagic acid. Reconstitution and subtraction of phytochemicals from the mixture indicate that Ellagic acid significantly contribute into anti-cancer effect of HCAE. Cancer cells treated with ellagic acid from HCAE were incapable of completing their cell-cycle and halted the cell-cycle at DNA synthesis S-Phase, as demonstrated by decreased cyclin A2 expression levels with increasing ellagic acid concentration. Halting of cells at S-phase causes induction of apoptosis in cancer cells. Cancer cells exhibiting DNA fragmentation, changes in expression of several apoptotic proteins such as Bcl2, cytochrome-c and formation of cleaved products of caspase 3 and PARP-1 suggests ellagic acid induces cell death via mitochondrial pathway of apoptosis.
    MeSH term(s) Humans ; Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Ellagic Acid/pharmacology ; HEK293 Cells ; Shikimic Acid ; Phytochemicals/pharmacology
    Chemical Substances Plant Extracts ; Ellagic Acid (19YRN3ZS9P) ; Shikimic Acid (29MS2WI2NU) ; Phytochemicals
    Language English
    Publishing date 2023-05-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0286274
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  8. Article ; Online: Publisher Correction: Optical reciprocity induced wavefront shaping for axial and lateral shifting of focus through a scattering medium.

    Sanjeev, Abhijit / Trivedi, Vismay / Zalevsky, Zeev

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 7525

    Language English
    Publishing date 2023-05-09
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-32998-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Molecular modelling, docking and network analysis of phytochemicals from Haritaki churna: role of protein cross-talks for their action.

    Khan, Md Rafi Uz Zama / Trivedi, Vishal

    Journal of biomolecular structure & dynamics

    2023  Volume 42, Issue 8, Page(s) 4297–4312

    Abstract: Phytochemicals are bioactive agents present in medicinal plants with therapeutic values. Phytochemicals isolated from plants target multiple cellular processes. In the current work, we have used fractionation techniques to identify 13 bioactive ... ...

    Abstract Phytochemicals are bioactive agents present in medicinal plants with therapeutic values. Phytochemicals isolated from plants target multiple cellular processes. In the current work, we have used fractionation techniques to identify 13 bioactive polyphenols in ayurvedic medicine Haritaki Churna. Employing the advanced spectroscopic and fractionation, structure of bioactive polyphenols was determined. Blasting the phytochemical structure allow us to identify a total of 469 protein targets from Drug bank and Binding DB. Phytochemicals with their protein targets from Drug bank was used to create a phytochemical-protein network comprising of 394 nodes and 1023 edges. It highlights the extensive cross-talk between protein target corresponding to different phytochemicals. Analysis of protein targets from Binding data bank gives a network comprised of 143 nodes and 275 edges. Taking the data together from Drug bank and binding data, seven most prominent drug targets (HSP90AA1, c-Src kinase, EGFR, Akt1, EGFR, AR, and ESR-α) were found to be target of the phytochemicals. Molecular modelling and docking experiment indicate that phytochemicals are fitting nicely into active site of the target proteins. The binding energy of the phytochemicals were better than the inhibitors of these protein targets. The strength and stability of the protein ligand complexes were further confirmed using molecular dynamic simulation studies. Further, the ADMET profiles of phytochemicals extracted from HCAE suggests that they can be potential drug targets. The phytochemical cross-talk was further proven by choosing c-Src as a model. HCAE down regulated c-Src and its downstream protein targets such as Akt1, cyclin D1 and vimentin. Hence, network analysis followed by molecular docking, molecular dynamics simulation and in-vitro studies clearly highlight the role of protein network and subsequent selection of drug candidate based on network pharmacology.Communicated by Ramaswamy H. Sarma.
    MeSH term(s) Molecular Docking Simulation ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Molecular Dynamics Simulation ; Protein Binding ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Polyphenols/chemistry ; Polyphenols/pharmacology ; Humans ; Models, Molecular ; Ligands ; Protein Interaction Maps/drug effects ; Proteins/chemistry ; Proteins/metabolism ; Medicine, Ayurvedic
    Chemical Substances Phytochemicals ; Plant Extracts ; Polyphenols ; Ligands ; Proteins
    Language English
    Publishing date 2023-06-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2023.2220036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2093: Show issues Location:
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  10. Article ; Online: Mapping morphogenesis and mechanics in embryo models.

    Liu, Zong-Yuan / Trivedi, Vikas / Heemskerk, Idse

    Nature methods

    2023  Volume 20, Issue 12, Page(s) 1859–1862

    Language English
    Publishing date 2023-12-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2169522-2
    ISSN 1548-7105 ; 1548-7091
    ISSN (online) 1548-7105
    ISSN 1548-7091
    DOI 10.1038/s41592-023-02079-4
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