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  1. Article ; Online: Bone involvement in non-congenital syphilis

    Trujillo, Daniela / Agudelo, Carlos Andrés / Chavarriaga, Andrés / Villa, Pablo / Vélez, Alejandro / Cardona, Ricardo / Hidrón, Alicia

    Biomedica : revista del Instituto Nacional de Salud

    2023  Volume 43, Issue 2, Page(s) 157–163

    Abstract: We documented two stages of bone involvement due to syphilis in two adult patients infected with human immunodeficiency virus. Bony lesions of secondary versus tertiary syphilis cannot be differentiated on clinical or radiologic grounds alone. Given the ... ...

    Title translation Compromiso óseo por sífilis no congénita
    Abstract We documented two stages of bone involvement due to syphilis in two adult patients infected with human immunodeficiency virus. Bony lesions of secondary versus tertiary syphilis cannot be differentiated on clinical or radiologic grounds alone. Given the rarity of this clinical presentation, there is no consensus on treatment duration and related outcomes.
    MeSH term(s) Adult ; Humans ; Syphilis/complications ; Syphilis/diagnosis ; Bone Diseases ; Consensus
    Language Spanish
    Publishing date 2023-06-30
    Publishing country Colombia
    Document type Journal Article
    ZDB-ID 2059952-3
    ISSN 2590-7379 ; 2590-7379
    ISSN (online) 2590-7379
    ISSN 2590-7379
    DOI 10.7705/biomedica.6603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)

    Jimenez, Jenyfer / Leiva, Ana Maria / Olaya, Cristian / Acosta-Trujillo, Daniela / Cuellar, Wilmer Jose

    MethodsX. 2021, v. 8 p.101496-

    2021  

    Abstract: Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these ... ...

    Abstract Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue.•DNA purity estimations reveal OD260/280 ratios above 2.0 and OD260/230 ratios above 1.7, even for samples stored in silica gel for several months.•The high quality of the extracts is suitable for detection of DNA and RNA viruses, with high efficiency.•We suggest this method could be used as part of a gold standard kit for virus detection in cassava.
    Keywords DNA ; Manihot esculenta ; RNA ; cassava ; cost effectiveness ; diagnostic techniques ; leaves ; liquid nitrogen ; monitoring ; pathogens ; phenol ; silica gel ; viruses ; CTAB extraction ; Disease surveillance ; Begomovirus ; Potexvirus ; Nucleic acid extraction protocol for the detection and characterization of cassava-infecting viruses
    Language English
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2021.101496
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: An optimized nucleic acid isolation protocol for virus diagnostics in cassava (

    Jimenez, Jenyfer / Leiva, Ana Maria / Olaya, Cristian / Acosta-Trujillo, Daniela / Cuellar, Wilmer Jose

    MethodsX

    2021  Volume 8, Page(s) 101496

    Abstract: Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these ... ...

    Abstract Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue.•DNA purity estimations reveal OD260/280 ratios above 2.0 and OD260/230 ratios above 1.7, even for samples stored in silica gel for several months.•The high quality of the extracts is suitable for detection of DNA and RNA viruses, with high efficiency.•We suggest this method could be used as part of a gold standard kit for virus detection in cassava.
    Language English
    Publishing date 2021-08-21
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2830212-6
    ISSN 2215-0161
    ISSN 2215-0161
    DOI 10.1016/j.mex.2021.101496
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: An optimized nucleic acid isolation protocol for virus diagnostics in cassava (Manihot esculenta Crantz.)

    Jiménez Polo, Jenyfer / Leiva, Ana Maria / Olaya, Cristian A. / Acosta-Trujillo, Daniela / Cuéllar, Wilmer Jose

    MethodsX

    2022  

    Abstract: Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these ... ...

    Abstract Our group works on the detection and characterization of cassava viruses, supporting projects that involve large scale pathogen surveillance activities and resistance screening assays in multiple and remote locations. In order to comply with these applications, nucleic acid isolation protocols need to be cost effective, adjusted for samples that will stand long distance transport and harsh storage conditions, while maximizing the yield and quality of the nucleic acid extracts obtained. The method we describe here has been widely used and validated using different downstream tests (including, but not limited to, Rolling Circle Amplification and Illumina and Nanopore sequencing), but is currently unpublished. The protocol begins with milligram amounts of dry leaf samples stored in silica gel, does not require liquid Nitrogen nor phenol extraction and produces an average of 2.11 µg of nucleic acids per mg of dry tissue.
    Keywords nucleic acids ; diagnostic techniques ; methods ; acidos nucléicos ; técnicas de diagnosis ; métodos
    Language English
    Publishing date 2022-08-25T07:39:11Z
    Publisher Elsevier
    Publishing country fr
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  5. Article ; Online: New Coordination Polymers of Copper(I) and Silver(I) with Pyrazine and Piperazine: A Step Toward "Green" Chemistry and Optoelectronic Applications.

    Almotawa, Ruaa M / Aljomaih, Ghadah / Trujillo, Daniela Vargas / Nesterov, Vladimir N / Rawashdeh-Omary, Manal A

    Inorganic chemistry

    2018  Volume 57, Issue 16, Page(s) 9962–9976

    Abstract: Five coordination polymers and one hexanuclear cluster have been obtained, and their crystal structures were determined upon reaction of Cu(I) or Ag(I) precursors with pyrazine (Pyz) or piperazine (Ppz). Five complexes are mixed-imine-ligand with anionic- ...

    Abstract Five coordination polymers and one hexanuclear cluster have been obtained, and their crystal structures were determined upon reaction of Cu(I) or Ag(I) precursors with pyrazine (Pyz) or piperazine (Ppz). Five complexes are mixed-imine-ligand with anionic-fluorinated pyrazolate [3,5-(CF
    Language English
    Publishing date 2018-08-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.8b01131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Primary Ciliary Dyskinesia as a Cause of Repeating Atelectasis in the Neonatal Period.

    Primo, Arlete Esteves Lopes / Brock Zacharias, Romy Schmidt / de Moraes, Amanda Dias / da Silva Filho, Luiz Vicente Ribeiro Ferreira / Trujillo, Daniela Rodriguez / Yoshida, Renata de Araújo Monteiro / Warth, Arno Noberto / Rebello, Celso Moura

    The American journal of case reports

    2020  Volume 21, Page(s) e921949

    Abstract: BACKGROUND Primary ciliary dyskinesia (PCD) is a disease characterized by motor ciliary dysfunction, which leads to the accumulation of secretions in the lower airways and, consequently, to atelectasis and repeated infections. During the neonatal period, ...

    Abstract BACKGROUND Primary ciliary dyskinesia (PCD) is a disease characterized by motor ciliary dysfunction, which leads to the accumulation of secretions in the lower airways and, consequently, to atelectasis and repeated infections. During the neonatal period, diagnosis can be difficult because the symptoms are frequently associated with other respiratory diseases common in neonates. The laterality defects should warn the clinician of the need for further investigation using clinical criteria, but the confirmation depends on a genetic test. CASE REPORT The objective of this report is to present a case of PCD manifesting in the neonatal period that was diagnosed due to respiratory failure associated with recurrent atelectasis and situs inversus totalis. CONCLUSIONS This disease is not well known by neonatologists, but early diagnosis decreases morbidity and improves patient quality of life.
    MeSH term(s) Administration, Inhalation ; Ciliary Motility Disorders/complications ; Ciliary Motility Disorders/therapy ; Continuous Positive Airway Pressure ; Diagnosis, Differential ; Humans ; Infant, Newborn ; Male ; Pulmonary Atelectasis/etiology ; Pulmonary Atelectasis/therapy ; Respiratory Distress Syndrome, Newborn/etiology ; Respiratory Distress Syndrome, Newborn/therapy ; Situs Inversus
    Language English
    Publishing date 2020-06-11
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 2517183-5
    ISSN 1941-5923 ; 1941-5923
    ISSN (online) 1941-5923
    ISSN 1941-5923
    DOI 10.12659/AJCR.921949
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Strengthening self-regulation and reducing poverty to prevent adolescent depression and anxiety: Rationale, approach and methods of the ALIVE interdisciplinary research collaboration in Colombia, Nepal and South Africa.

    Lund, Crick / Jordans, Mark J D / Garman, Emily / Araya, Ricardo / Avendano, Mauricio / Bauer, Annette / Bahure, Vikram / Dua, Tarun / Eleftheriou, Georgia / Evans-Lacko, Sara / García Rodríguez, Juan Felipe / Gautam, Kamal / Gevonden, Martin / Hessel, Philipp / Kohrt, Brandon A / Krabbendam, Lydia / Luitel, Nagendra P / Roy, Sanchari / Seifert Bonifaz, Manuel /
    Singh, Rakesh / Sinichi, Mohammadamin / Sorsdahl, Katherine / Thornicroft, Graham / Tol, Wietse A / Trujillo, Daniela / van der Merwe, Nicci / Wahid, Syed Shabab / Yarrow, Paula

    Epidemiology and psychiatric sciences

    2023  Volume 32, Page(s) e69

    Abstract: Aims: Depression and anxiety are the leading contributors to the global burden of disease among young people, accounting for over a third (34.8%) of years lived with disability. Yet there is limited evidence for interventions that prevent adolescent ... ...

    Abstract Aims: Depression and anxiety are the leading contributors to the global burden of disease among young people, accounting for over a third (34.8%) of years lived with disability. Yet there is limited evidence for interventions that prevent adolescent depression and anxiety in low- and middle-income countries (LMICs), where 90% of adolescents live. This article introduces the 'Improving Adolescent mentaL health by reducing the Impact of poVErty (ALIVE)' study, its conceptual framework, objectives, methods and expected outcomes. The aim of the ALIVE study is to develop and pilot-test an intervention that combines poverty reduction with strengthening self-regulation to prevent depression and anxiety among adolescents living in urban poverty in Colombia, Nepal and South Africa.
    Methods: This aim will be achieved by addressing four objectives: (1) develop a conceptual framework that identifies the causal mechanisms linking poverty, self-regulation and depression and anxiety; (2) develop a multi-component selective prevention intervention targeting self-regulation and poverty among adolescents at high risk of developing depression or anxiety; (3) adapt and validate instruments to measure incidence of depression and anxiety, mediators and implementation parameters of the prevention intervention; and (4) undertake a four-arm pilot cluster randomised controlled trial to assess the feasibility, acceptability and cost of the selective prevention intervention in the three study sites.
    Results: The contributions of this study include the active engagement and participation of adolescents in the research process; a focus on the causal mechanisms of the intervention; building an evidence base for prevention interventions in LMICs; and the use of an interdisciplinary approach.
    Conclusions: By developing and evaluating an intervention that addresses multidimensional poverty and self-regulation, ALIVE can make contributions to evidence on the integration of mental health into broader development policy and practice.
    MeSH term(s) Adolescent ; Humans ; Anxiety/prevention & control ; Anxiety/psychology ; Colombia/epidemiology ; Depression/psychology ; Interdisciplinary Research ; Nepal ; Poverty ; Self-Control ; South Africa/epidemiology
    Language English
    Publishing date 2023-12-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2607964-1
    ISSN 2045-7979 ; 2045-7960
    ISSN (online) 2045-7979
    ISSN 2045-7960
    DOI 10.1017/S2045796023000811
    Database MEDical Literature Analysis and Retrieval System OnLINE

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