Article ; Online: Ba-Wei-Die-Huang-Wan (Hachimi-jio-gan) can ameliorate ketamine-induced cystitis by modulating neuroreceptors, inflammatory mediators, and fibrogenesis in a rat model.
2019 Volume 38, Issue 8, Page(s) 2159–2169
Abstract: Aim: We investigated the effects of Ba-Wei-Die-Huang-Wan (BWDHW) on ketamine-induced cystitis (KIC) in a rat model.: Methods: Female Sprague-Dawley rats were distributed into three groups: control (saline), ketamine (25 mg/kg/day for 28 days), or ... ...
| Abstract | Aim: We investigated the effects of Ba-Wei-Die-Huang-Wan (BWDHW) on ketamine-induced cystitis (KIC) in a rat model. Methods: Female Sprague-Dawley rats were distributed into three groups: control (saline), ketamine (25 mg/kg/day for 28 days), or ketamine (25 mg/kg/day for 28 days) plus BWDHW (90 mg/kg/day, started from day 14). Functional magnetic resonance imaging (fMRI), metabolic cage study, and cystometry were evaluated. Bladder histology was evaluated. Western blots of the bladder proteins were carried out. Results: Compared with controls, ketamine-treated rats showed stronger fMRI intensity in the periaqueductal gray area and bladder overactivity in the bladder functional study, but the ketamine/BWDHW-treated rats did not. Furthermore, ketamine breached the uroplakin III membrane at the apical surface of the urothelium, enhanced substance P spread over the urothelium, induced suburothelial hemorrhage and monocyte/macrophage infiltration, and caused interstitial fibrosis deposition. By contrast, the BWDHW-treated rats exhibited less substance P spread, lower suburothelial monocyte/macrophage infiltration, and lower interstitial fibrosis deposition. The ketamine group showed significant overexpression of neuroreceptors in the bladder mucosa (the transient receptor potential vanilloid 1 and M Conclusion: BWDHW could exert therapeutic effects by inhibiting the upregulation of neuroreceptors, modulating inflammatory mediators, suppressing fibrogenesis, and ameliorating bladder overactivity in rats with KIC. |
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| MeSH term(s) | Animals ; Collagen/drug effects ; Collagen/metabolism ; Cyclooxygenase 2/drug effects ; Cyclooxygenase 2/metabolism ; Cystitis/chemically induced ; Cystitis/metabolism ; Cystitis/pathology ; Cystitis/physiopathology ; Drugs, Chinese Herbal/pharmacology ; Female ; Fibronectins/drug effects ; Fibronectins/metabolism ; Functional Neuroimaging ; Intercellular Adhesion Molecule-1/drug effects ; Intercellular Adhesion Molecule-1/metabolism ; Interleukin-1beta/drug effects ; Interleukin-1beta/metabolism ; Interleukin-6/metabolism ; Ketamine/adverse effects ; Magnetic Resonance Imaging ; NF-kappa B/drug effects ; NF-kappa B/metabolism ; Periaqueductal Gray/diagnostic imaging ; Rats ; Rats, Sprague-Dawley ; Receptors, Muscarinic/drug effects ; Receptors, Muscarinic/metabolism ; Sensory Receptor Cells ; Substance P/drug effects ; Substance P/metabolism ; TRPV Cation Channels/drug effects ; TRPV Cation Channels/metabolism ; Transforming Growth Factor beta1/drug effects ; Transforming Growth Factor beta1/metabolism ; Tumor Necrosis Factor-alpha/drug effects ; Tumor Necrosis Factor-alpha/metabolism ; Urinary Bladder/drug effects ; Urinary Bladder/metabolism ; Urinary Bladder/pathology ; Urinary Bladder/physiopathology ; Urinary Bladder, Overactive/chemically induced ; Urinary Bladder, Overactive/metabolism ; Urinary Bladder, Overactive/pathology ; Urinary Bladder, Overactive/physiopathology ; Urothelium/drug effects ; Urothelium/metabolism |
| Chemical Substances | Drugs, Chinese Herbal ; Fibronectins ; IL1B protein, rat ; Il6 protein, rat ; Interleukin-1beta ; Interleukin-6 ; NF-kappa B ; Receptors, Muscarinic ; TRPV Cation Channels ; TRPV1 receptor ; Tgfb1 protein, rat ; Transforming Growth Factor beta1 ; Tumor Necrosis Factor-alpha ; hachimijiogan ; Intercellular Adhesion Molecule-1 (126547-89-5) ; Substance P (33507-63-0) ; Ketamine (690G0D6V8H) ; Collagen (9007-34-5) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Ptgs2 protein, rat (EC 1.14.99.1) |
| Language | English |
| Publishing date | 2019-09-20 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 604904-7 |
| ISSN | 1520-6777 ; 0733-2467 |
| ISSN (online) | 1520-6777 |
| ISSN | 0733-2467 |
| DOI | 10.1002/nau.24165 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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