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Article ; Online: Reproductive and Developmental Alterations on Zebrafish (Danio rerio) Upon Long-Term Exposure of Di-ethyl phthalate (DEP), Di-isononyl phthalate (DINP) and Di (2-ethylhexyl) phthalate (DEHP)

Tsai, Shu Chuan / Tseng, Yu-Jen / Wu, Sumei

Bull Environ Contam Toxicol. 2023 Feb., v. 110, no. 2 p.49-49

2023

Abstract: Di (2-ethylhexyl) phthalate (DEHP), di-ethyl phthalate (DEP) and di-isononyl phthalate (DINP) are all endocrine disrupting chemicals (EDCs) for organisms. However, little research has been done on the effects of long-term EDC exposure. The present study ... ...

Abstract	Di (2-ethylhexyl) phthalate (DEHP), di-ethyl phthalate (DEP) and di-isononyl phthalate (DINP) are all endocrine disrupting chemicals (EDCs) for organisms. However, little research has been done on the effects of long-term EDC exposure. The present study found that the zebrafish barely grew during the 7 months of DINP exposure. The fecundity rate (%) of female spawning was lower in the DEHP treatment by 4 months compared to other exposure groups. Zebrafish treated with 12.5–25.0 ppm of DEP for 4 months presented no spawning. Gonadal-somatic index (GSI) levels significantly decreased, and there were more oocytes in the atresia and peri-nucleus stage compared to the control group. In addition, the hatching rate of embryos were 71.02%, 56.92%, and 21.70% for females treated with DINP, DEHP and DEP, respectively. There were also abnormal craniofacial chondrogenesis development on 72 hpf embryos upon females treated with the three EDCs. In conclusion, long term exposure of DEHP, DINP, and DEP did not only affect the reproductive capacity of female zebrafish, but the 3 plasticizers also influence craniofacial cartilage development of its offspring.
Keywords	Danio rerio ; abnormal development ; bulls ; cartilage ; chondrogenesis ; chronic exposure ; fecundity ; females ; oocytes ; phthalates ; progeny ; reproductive performance
Language	English
Dates of publication	2023-02
Size	p. 49.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	6895-0
ISSN	1432-0800 ; 0007-4861
ISSN (online)	1432-0800
ISSN	0007-4861
DOI	10.1007/s00128-023-03689-6
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Reproductive and Developmental Alterations on Zebrafish (Danio rerio) Upon Long-Term Exposure of Di-ethyl phthalate (DEP), Di-isononyl phthalate (DINP) and Di (2-ethylhexyl) phthalate (DEHP).

Tsai, Shu Chuan / Tseng, Yu-Jen / Wu, Su Mei

Bulletin of environmental contamination and toxicology

2023 Volume 110, Issue 2, Page(s) 49

Abstract	Di (2-ethylhexyl) phthalate (DEHP), di-ethyl phthalate (DEP) and di-isononyl phthalate (DINP) are all endocrine disrupting chemicals (EDCs) for organisms. However, little research has been done on the effects of long-term EDC exposure. The present study found that the zebrafish barely grew during the 7 months of DINP exposure. The fecundity rate (%) of female spawning was lower in the DEHP treatment by 4 months compared to other exposure groups. Zebrafish treated with 12.5-25.0 ppm of DEP for 4 months presented no spawning. Gonadal-somatic index (GSI) levels significantly decreased, and there were more oocytes in the atresia and peri-nucleus stage compared to the control group. In addition, the hatching rate of embryos were 71.02%, 56.92%, and 21.70% for females treated with DINP, DEHP and DEP, respectively. There were also abnormal craniofacial chondrogenesis development on 72 hpf embryos upon females treated with the three EDCs. In conclusion, long term exposure of DEHP, DINP, and DEP did not only affect the reproductive capacity of female zebrafish, but the 3 plasticizers also influence craniofacial cartilage development of its offspring.
MeSH term(s)	Animals ; Female ; Diethylhexyl Phthalate/toxicity ; Zebrafish ; Reproduction ; Phthalic Acids/toxicity ; Plasticizers/toxicity ; Endocrine Disruptors/toxicity
Chemical Substances	phthalic acid (6O7F7IX66E) ; Diethylhexyl Phthalate (C42K0PH13C) ; Phthalic Acids ; Plasticizers ; Endocrine Disruptors
Language	English
Publishing date	2023-01-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	6895-0
ISSN	1432-0800 ; 0007-4861
ISSN (online)	1432-0800
ISSN	0007-4861
DOI	10.1007/s00128-023-03689-6
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Article: Generational effects and abnormalities in craniofacial chondrogenesis in zebrafish (Danio rerio) embryos upon maternal exposure to estrogen endocrine disrupting chemicals.

Tseng, Yu-Jen / Lu, Fu-I / Wu, Su Mei

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

2023 Volume 273, Page(s) 109743

Abstract: Bisphenol A (BPA) and diethyl phthalate (DEP) are estrogenic endocrine disrupting chemicals (EEDCs). The present study reconfirmed that the angle of the ceratohyal cartilage (CH) in embryos were larger from maternal BPA and E2, but smaller from DEP ... ...

Abstract	Bisphenol A (BPA) and diethyl phthalate (DEP) are estrogenic endocrine disrupting chemicals (EEDCs). The present study reconfirmed that the angle of the ceratohyal cartilage (CH) in embryos were larger from maternal BPA and E2, but smaller from DEP compared to the control. However, it is still unknown whether both the BPA and DEP chemicals disrupted the action of E2 and thereby influence the estrogen signaling pathways. Additionally, it remains unclear whether they also disrupted certain related genes in the migratory pathways of neural crest cells (NCCs) in their offspring. The present data showed that nuclear estrogen receptors and membrane estrogen receptors have different disrupted profiles among female zebrafish exposed to BPA (F-BPA), and DEP (F-DEP), and external E2 (F-E2). However, certain related genes in the migratory pathways of NCCs in embryos from F-BPA and F-E2 such as the sox10, chm1, and tgfbr1a mRNA expressions showed a positive relationship compared with CH angles; the gene expressions of sox9a, smad3, and col2a1a and the CH angles of embryos exhibited an opposite relationship upon F-DEP treatments. Thus, we suggested that the genes involved in NCCs migration were potentially induced by the residual maternal DEP contents. Two sets of genes, chm1/tgfb3 and chm1/gper1, exhibited an identical profile in the ovary and its offspring at 2 h of post fertilization upon F-E2 and F-BPA treatments, respectively. We suggested that the maternal mRNA from female to embryos were transferred before the maternal-to-zygotic transition stage.
MeSH term(s)	Female ; Animals ; Humans ; Receptors, Estrogen/genetics ; Chondrogenesis ; Zebrafish/genetics ; Maternal Exposure/adverse effects ; Endocrine Disruptors/toxicity ; Estrogens/toxicity
Chemical Substances	Receptors, Estrogen ; Endocrine Disruptors ; Estrogens ; bisphenol A (MLT3645I99)
Language	English
Publishing date	2023-09-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	189285-x
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
DOI	10.1016/j.cbpc.2023.109743
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Article: Maternal effect and dietary supplementation of estradiol-17β on female zebrafish (Danio rerio) affects the swimming behavior and stress-coping styles of its offspring.

Wu, Su Mei / Tseng, Yu-Jen / Chen, Te-Hao

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

2021 Volume 252, Page(s) 109211

Abstract: Extrinsic estradiol-17β (E2) is an environmental hormone. Female fish exposure to waterborne E2 might affect the development of craniofacial cartilage of its offspring. The present study investigates the effects of maternal E2 on larval craniofacial ... ...

Abstract	Extrinsic estradiol-17β (E2) is an environmental hormone. Female fish exposure to waterborne E2 might affect the development of craniofacial cartilage of its offspring. The present study investigates the effects of maternal E2 on larval craniofacial cartilage development by administering oral feed containing E2 (F-E2) to female zebrafish, and examines whether the swimming behavior and their stress coping style are influenced by maternal E2. The results showed that E2 contents responded to dosage and time in male fish after being fed with a diet containing E2. In addition, the E2 contents in female ovaries showed a significant increase after 250 mg of E2/kg treatment for 14 d. On the other hand, the fecundity rate of F-E2 group was lower around 2 folds than FC (female fed 0 mg of E2/kg) group. Craniofacial chondrogenesis on 72 hpf (hours of post fertilization) of F-E2 larvae were abnormalities, and a recovery to a normal developmental pattern was observed at the 96 hpf stage. The swimming speed was slower for F-E2 larvae compared to the FC larvae; and the F-E2 juvenile seems to be less responsive to cortisol (LRC) after cold stress. According to the results, we suggested that F-E2 larvae might have worse environmental adaptability than FC larvae.
MeSH term(s)	Animals ; Behavior, Animal/drug effects ; Chondrogenesis/drug effects ; Cold Temperature ; Craniofacial Abnormalities/chemically induced ; Diet ; Estradiol/pharmacology ; Female ; Larva/drug effects ; Maternal Exposure ; Stress, Physiological/drug effects ; Swimming ; Zebrafish
Chemical Substances	Estradiol (4TI98Z838E)
Language	English
Publishing date	2021-10-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	189285-x
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
DOI	10.1016/j.cbpc.2021.109211
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Maternal effect and dietary supplementation of estradiol-17β on female zebrafish (Danio rerio) affects the swimming behavior and stress-coping styles of its offspring

Wu, Su Mei / Tseng, Yu-Jen / Chen, Te-Hao

Comparative biochemistry and physiology. 2022 Feb., v. 252

2022

Abstract	Extrinsic estradiol-17β (E2) is an environmental hormone. Female fish exposure to waterborne E2 might affect the development of craniofacial cartilage of its offspring. The present study investigates the effects of maternal E2 on larval craniofacial cartilage development by administering oral feed containing E2 (F-E2) to female zebrafish, and examines whether the swimming behavior and their stress coping style are influenced by maternal E2. The results showed that E2 contents responded to dosage and time in male fish after being fed with a diet containing E2. In addition, the E2 contents in female ovaries showed a significant increase after 250 mg of E2/kg treatment for 14 d. On the other hand, the fecundity rate of F-E2 group was lower around 2 folds than FC (female fed 0 mg of E2/kg) group. Craniofacial chondrogenesis on 72 hpf (hours of post fertilization) of F-E2 larvae were abnormalities, and a recovery to a normal developmental pattern was observed at the 96 hpf stage. The swimming speed was slower for F-E2 larvae compared to the FC larvae; and the F-E2 juvenile seems to be less responsive to cortisol (LRC) after cold stress. According to the results, we suggested that F-E2 larvae might have worse environmental adaptability than FC larvae.
Keywords	Danio rerio ; cartilage ; chondrogenesis ; cold stress ; cortisol ; dietary supplements ; fecundity ; females ; fish ; juveniles ; larvae ; males ; maternal effect ; progeny
Language	English
Dates of publication	2022-02
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	189285-x
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
DOI	10.1016/j.cbpc.2021.109211
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Article: Effects of bisphenol A or diethyl phthalate on cartilage development and the swimming behavior of zebrafish (Danio rerio) through maternal exposure.

Tseng, Yu-Jen / Chen, Te-Hao / Tsai, Shu Chuan / Wu, Su Mei

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

2021 Volume 247, Page(s) 109057

Abstract: Waterborne bisphenol A (BPA) and diethyl phthalate (DEP) are endocrine disruptive chemicals that impact the reproductive system of fish. The present study checks the effectiveness of the reproductive capacity on zebrafish after BPA and DEP exposure, and ... ...

Abstract	Waterborne bisphenol A (BPA) and diethyl phthalate (DEP) are endocrine disruptive chemicals that impact the reproductive system of fish. The present study checks the effectiveness of the reproductive capacity on zebrafish after BPA and DEP exposure, and consequently investigates its effect on their development and the swimming behavior of its offspring. The exposure of BPA and DEP to zebrafish reveals that the levels of ovarian 17β-estradiol (E2) and relative mRNA expression (RRE) ratios (Treatment/Control) of hepatic vitellogenin (vtg1) could be induced and decreased. Liver RRE levels in estrogen receptors (ERs) are also affected. Among the ERs, esr2a significantly increased upon BPA exposure, and esr1 and esr2b decreased upon DEP exposure. In addition, the ceratohyal cartilage (CH) angle of larvae whose mothers were exposed to BPA (F-BPA) was significantly bigger, but the CH angle of larvae whose mothers were exposed to DEP (F-DEP) was significantly smaller than the control. The swimming performance of larvae from F-DEP was more compromised than the control, but the situation did not appear in the larvae from the F-BPA group. The success rate of larvae hatching from F-BPA and F-DEP was lower than control group. Moreover, the successful rate of female spawns was higher in the control group compared to the treatment groups exposed to BPA and DEP. We suggested that both maternal BPA and DEP disrupt E2 levels, and influence the CH development of larvae, resulting in a decrease in successful hatching. Only the swimming behavior of larvae from maternal DEP was disrupted.
MeSH term(s)	Animals ; Behavior, Animal/drug effects ; Benzhydryl Compounds/toxicity ; Embryo, Nonmammalian/drug effects ; Endocrine Disruptors/toxicity ; Female ; Maternal Exposure/adverse effects ; Phenols/toxicity ; Phthalic Acids/toxicity ; Water Pollutants, Chemical/toxicity ; Zebrafish/embryology
Chemical Substances	Benzhydryl Compounds ; Endocrine Disruptors ; Phenols ; Phthalic Acids ; Water Pollutants, Chemical ; bisphenol A (MLT3645I99) ; diethyl phthalate (UF064M00AF)
Language	English
Publishing date	2021-04-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	189285-x
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
ISSN	1532-0456 ; 0306-4492 ; 0742-8413
DOI	10.1016/j.cbpc.2021.109057
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Effects of bisphenol A or diethyl phthalate on cartilage development and the swimming behavior of zebrafish (Danio rerio) through maternal exposure

Tseng, Yu-Jen / Chen, Te-Hao / Tsai, Shu Chuan / Wu, Su Mei

Comparative biochemistry and physiology. 2021 Sept., v. 247

2021

Abstract	Waterborne bisphenol A (BPA) and diethyl phthalate (DEP) are endocrine disruptive chemicals that impact the reproductive system of fish. The present study checks the effectiveness of the reproductive capacity on zebrafish after BPA and DEP exposure, and consequently investigates its effect on their development and the swimming behavior of its offspring. The exposure of BPA and DEP to zebrafish reveals that the levels of ovarian 17β-estradiol (E2) and relative mRNA expression (RRE) ratios (Treatment/Control) of hepatic vitellogenin (vtg1) could be induced and decreased. Liver RRE levels in estrogen receptors (ERs) are also affected. Among the ERs, esr2a significantly increased upon BPA exposure, and esr1 and esr2b decreased upon DEP exposure. In addition, the ceratohyal cartilage (CH) angle of larvae whose mothers were exposed to BPA (F-BPA) was significantly bigger, but the CH angle of larvae whose mothers were exposed to DEP (F-DEP) was significantly smaller than the control. The swimming performance of larvae from F-DEP was more compromised than the control, but the situation did not appear in the larvae from the F-BPA group. The success rate of larvae hatching from F-BPA and F-DEP was lower than control group. Moreover, the successful rate of female spawns was higher in the control group compared to the treatment groups exposed to BPA and DEP. We suggested that both maternal BPA and DEP disrupt E2 levels, and influence the CH development of larvae, resulting in a decrease in successful hatching. Only the swimming behavior of larvae from maternal DEP was disrupted.
Keywords	Danio rerio ; bisphenol A ; cartilage ; diethyl phthalate ; females ; fish ; gene expression ; liver ; maternal exposure ; progeny ; reproductive performance ; vitellogenin
Language	English
Dates of publication	2021-09
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	189285-x
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
ISSN	0306-4492 ; 0742-8413 ; 1532-0456
DOI	10.1016/j.cbpc.2021.109057
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Article ; Online: Friend or foe: an unusual case of small intestinal mass.

Tseng, Yu-Jen / Ding, Wei-Qun / Zhong, Liang / Luo, Zhong-Guang

Endoscopy

2019 Volume 51, Issue 6, Page(s) E156–E157

MeSH term(s)	Aged ; Bezoars/diagnosis ; Bezoars/therapy ; Diagnosis, Differential ; Endoscopy, Gastrointestinal ; Female ; Humans ; Intestine, Small ; Lithotripsy ; Tomography, X-Ray Computed
Language	English
Publishing date	2019-04-01
Publishing country	Germany
Document type	Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	80120-3
ISSN	1438-8812 ; 0013-726X
ISSN (online)	1438-8812
ISSN	0013-726X
DOI	10.1055/a-0861-9980
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Article: miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes.

Wang, Jing-Jie / Zhang, Yi-Tong / Tseng, Yu Jen / Zhang, Jun

Hepatobiliary & pancreatic diseases international : HBPD INT

2019 Volume 18, Issue 4, Page(s) 360–365

Abstract: Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases. However, the exact pathogenesis of NAFLD remains to be elucidated. Despite the association with tumors and cardiovascular diseases, the role of ... ...

Abstract	Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most prevalent chronic liver diseases. However, the exact pathogenesis of NAFLD remains to be elucidated. Despite the association with tumors and cardiovascular diseases, the role of miR-222 in NAFLD remains unclear. The present study was to investigate the role of miR-222 in NAFLD. Methods: Wild-type C57BL/6 mice were fed a high-fat diet for 12 weeks to induce NAFLD. Normal human liver cell line (L02) was cultured with free fatty acid (FFA)-containing medium to stimulate cell steatosis. The mRNA levels of miR-222 and acyl Coenzyme A xidase 1 (ACOX1) were detected by quantitative-PCR (Q-PCR). The prediction of ACOX1 as the target gene for miR-222 was conducted via TargetScan. The overexpression or inhibition of miR-222 was mediated by miR-222 mimics or antagomir, and intracellular triglyceride levels were measured using a triglyceride kit. Luciferase reporter assays verified ACOX1 as the target gene for miR-222. Results: miR-222 was significantly elevated in both the in vivo and in vitro NAFLD models. Overexpression of miR-222 significantly increased triglyceride content in the L02 cells, while inhibition of miR-222 expression restricted the accumulation of triglyceride. Overexpression of miR-222 significantly inhibited ACOX1 expression. Transient transfection assays verified that ACOX1 3'-UTR luciferase reporter activity could be inhibited by miR-222 overexpression. Conclusions: The present study suggested that miR-222 promotes the accumulation of triglycerides by inhibiting ACOX1.
MeSH term(s)	3' Untranslated Regions ; Acyl-CoA Oxidase/genetics ; Acyl-CoA Oxidase/metabolism ; Animals ; Binding Sites ; Cell Line ; Diet, High-Fat ; Disease Models, Animal ; Gene Expression Regulation, Enzymologic ; Hepatocytes/enzymology ; Hepatocytes/pathology ; Humans ; Male ; Mice, Inbred C57BL ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Non-alcoholic Fatty Liver Disease/enzymology ; Non-alcoholic Fatty Liver Disease/genetics ; Non-alcoholic Fatty Liver Disease/pathology ; Oxidoreductases/genetics ; Oxidoreductases/metabolism ; Triglycerides/metabolism ; Up-Regulation
Chemical Substances	3' Untranslated Regions ; MIRN222 microRNA, human ; MIRN222 microRNA, mouse ; MicroRNAs ; Triglycerides ; Oxidoreductases (EC 1.-) ; ACOX2 protein, human (EC 1.17.99.3) ; ACOX1 protein, mouse (EC 1.3.3.6) ; Acyl-CoA Oxidase (EC 1.3.3.6)
Language	English
Publishing date	2019-05-13
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2241386-8
ISSN	1499-3872
ISSN	1499-3872
DOI	10.1016/j.hbpd.2019.05.002
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Article: Six immune-related promising biomarkers may promote hepatocellular carcinoma prognosis: a bioinformatics analysis and experimental validation.

Lin, Xia-Hui / Li, Dong-Ping / Liu, Zhi-Yong / Zhang, Si / Tang, Wen-Qing / Chen, Rong-Xin / Weng, Shu-Qiang / Tseng, Yu-Jen / Xue, Ru-Yi / Dong, Ling

Cancer cell international

2023 Volume 23, Issue 1, Page(s) 52

Abstract: Background: Abnormal miRNA and mRNA expression and dysregulated immune microenvironment have been found to frequently induce the progression of hepatocellular carcinoma (HCC) in recent reports. In particular, the immune-related competing endogenous RNAs ...

Abstract	Background: Abnormal miRNA and mRNA expression and dysregulated immune microenvironment have been found to frequently induce the progression of hepatocellular carcinoma (HCC) in recent reports. In particular, the immune-related competing endogenous RNAs (ceRNA) mechanism plays a crucial role in HCC progression. However, the underlying mechanisms remain unclear. Methods: Differentially expressed immune-related genes were obtained from the Immport, GEO, and TCGA databases. The mRNA and protein expression levels in HCC tissues and adjacent normal tissues were confirmed, and we further investigated the methylation levels of these biomarkers to explore their function. Then, the TIMER and TISCH databases were used to assess the relationship between immune infiltration and hub genes. Survival analysis and univariate and multivariate Cox models were used to evaluate the association between hub genes and HCC diagnosis. Hub gene expression was experimentally validated in six HCC cell lines and 15 HCC samples using qRT-PCR and immunohistochemistry. The hub genes were uploaded to DSigDB for drug prediction enrichment analysis. Results: We identified that patients with abnormal miRNAs (hsa-miR-125b-5p and hsa-miR-21-5p) and their targeted genes (NTF3, PSMD14, CD320, and SORT1) had a worse prognosis. Methylation analysis of miRNA-targeted genes suggested that alteration of methylation levels is also a factor in the induction of tumorigenesis. We also found that the development of HCC progression caused by miRNA-mRNA interactions may be closely correlated with the infiltration of immunocytes. Moreover, the GSEA, GO, and KEGG analysis suggested that several common immune-related biological processes and pathways were related to miRNA-targeted genes. The results of qRT-PCR, immunohistochemistry, and western blotting were consistent with our bioinformatics results, suggesting that abnormal miRNAs and their targeted genes may affect HCC progression. Conclusions: Briefly, our study systematically describes the mechanisms of miRNA-mRNA interactions in HCC and predicts promising biomarkers that are associated with immune filtration for HCC progression.
Language	English
Publishing date	2023-03-23
Publishing country	England
Document type	Journal Article
ZDB-ID	2091573-1
ISSN	1475-2867
ISSN	1475-2867
DOI	10.1186/s12935-023-02888-9
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