LIVIVO - Search results -

Search results

Result 1 - 10 of total 24

Search options

Article ; Online: Quantitative mRNA expression measurement at home

Sonalisa Pandey / Sara Safa McCoy / Tsering Stobdan / Debashis Sahoo

Scientific Reports, Vol 14, Iss 1, Pp 1-

2024 Volume 6

Abstract: Abstract mRNA measurement is dominated by RT-PCR, which requires expensive laboratory equipment and personnel with advanced degrees. Loop-mediated isothermal amplification (LAMP) is a versatile technique for detecting target DNA and RNA. The sensitivity ... ...

Abstract	Abstract mRNA measurement is dominated by RT-PCR, which requires expensive laboratory equipment and personnel with advanced degrees. Loop-mediated isothermal amplification (LAMP) is a versatile technique for detecting target DNA and RNA. The sensitivity of LAMP in early reports has been below that of the standard RT-PCR tests. Here, we report the use of a fluorescence-based RT-LAMP protocol to measure CDX2 expression patterns, which match extremely well to the standards of sophisticated RT-PCR techniques (r = 0.99, p < 0.001). The assay works on diverse sample types such as cDNA, mRNA, and direct tissue sample testing in 25 min compared to more than 3 h for RT-PCR. We have developed a new protocol for designing RT-LAMP primers that reduce false positives due to self-amplification and improve quantification. A simple device with a 3D-printed box enables the measurement of mRNA expression at home, outdoors, and point-of-care setting.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A Boolean approach for novel hypoxia-related gene discovery.

Tsering Stobdan / Debashis Sahoo / Gabriel G Haddad

PLoS ONE, Vol 17, Iss 8, p e

2022 Volume 0273524

Abstract: Hypoxia plays a major role in the etiology and pathogenesis of most of the leading causes of morbidity and mortality, whether cardiovascular diseases, cancer, respiratory diseases or stroke. Despite active research on hypoxia-signaling pathways, the ... ...

Abstract	Hypoxia plays a major role in the etiology and pathogenesis of most of the leading causes of morbidity and mortality, whether cardiovascular diseases, cancer, respiratory diseases or stroke. Despite active research on hypoxia-signaling pathways, the understanding of regulatory mechanisms, especially in specific tissues, still remain elusive. With the accessibility of thousands of potentially diverse genomic datasets, computational methods are utilized to generate new hypotheses. Here we utilized Boolean implication relationship, a powerful method to probe symmetrically and asymmetrically related genes, to identify novel hypoxia related genes. We used a well-known hypoxia-responsive gene, VEGFA, with very large human expression datasets (n = 25,955) to identify novel hypoxia-responsive candidate gene/s. Further, we utilized in-vitro analysis using human endothelial cells exposed to 1% O2 environment for 2, 8, 24 and 48 hours to validate top candidate genes. Out of the top candidate genes (n = 19), 84% genes were previously reported as hypoxia related, validating our results. However, we identified FAM114A1 as a novel candidate gene significantly upregulated in the endothelial cells at 8, 24 and 48 hours of 1% O2 environment. Additional evidence, particularly the localization of intronic miRNA and numerous HREs further support and strengthen our finding. Current results on FAM114A1 provide an example demonstrating the utility of powerful computational methods, like Boolean implications, in playing a major role in hypothesis building and discovery.
Keywords	Medicine ; R ; Science ; Q
Subject code	610
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Multiple mechanisms drive genomic adaptation to extreme O2 levels in Drosophila melanogaster

Arya Iranmehr / Tsering Stobdan / Dan Zhou / Huiwen Zhao / Sergey Kryazhimskiy / Vineet Bafna / Gabriel G. Haddad

Nature Communications, Vol 12, Iss 1, Pp 1-

2021 Volume 10

Abstract: The genomic details of adaptation to extreme environments remain challenging to characterize. Using new methods to analyze flies experimentally evolved to survive extreme O2 conditions, the authors find a surprising level of synchronicity in selective ... ...

Abstract	The genomic details of adaptation to extreme environments remain challenging to characterize. Using new methods to analyze flies experimentally evolved to survive extreme O2 conditions, the authors find a surprising level of synchronicity in selective sweeps, de novo mutations and adaptive recombination events.
Keywords	Science ; Q
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Black Polyethylene Mulch Results in Over Two-Fold Increase in Capsicum (Capsicum annuum L.) Yield in Trans-Himalaya

Angmo, Stanzin / Bhuvnesh Kumar / Diskit Dolkar / Tsering Stobdan / Tsewang Norbu

National Academy science letters. 2018 June, v. 41, no. 3

2018

Abstract: The influence of black polyethylene mulch (BPM) on growth and yield of capsicum, a warm season crop, was investigated under a low-input cultivation system in trans-Himalayan Ladakh at an elevation of 3344 m. The mean marketable yield in different ... ...

Abstract	The influence of black polyethylene mulch (BPM) on growth and yield of capsicum, a warm season crop, was investigated under a low-input cultivation system in trans-Himalayan Ladakh at an elevation of 3344 m. The mean marketable yield in different treatments varied from 0.6 ± 0.0 to 9.2 ± 0.5 and 0.3 ± 0.1 to 5.2 ± 1.5 t ha−1 in 2014 and 2015, respectively. BPM increased marketable yield by 1.3- to 9.0-fold depending on year and crop variety. The mean marketable yield of four varieties under BPM increased by 2.8- and 2.9-fold in 2014 and 2015, respectively. The best performing variety (California Wonder) under BPM yielded 7.2 t ha−1 marketable fruit, which suggested that capsicum can be grown in open field condition in trans-Himalayan regions. Temperature 10 cm beneath the BPM was 3.1 ± 0.3 oC higher than in nonmulched soil. BPM reduced 68% weed and save 75% time in manual weeding. Incidence of insect-pest and diseases was minimal, and rotten fruit was less than 1% of marketable yield without application of pesticide or fungicide.
Keywords	Capsicum annuum ; cultivars ; fruits ; fungicides ; plastic film mulches ; soil ; temperature ; warm season ; weeds
Language	English
Dates of publication	2018-06
Size	p. 173-176.
Publishing place	Springer India
Document type	Article
ZDB-ID	2677544-X
ISSN	2250-1754 ; 0250-541X
ISSN (online)	2250-1754
ISSN	0250-541X
DOI	10.1007/s40009-018-0637-y
Database	NAL-Catalogue (AGRICOLA)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Stress tolerance and plant growth promotion potential of Enterobacter ludwigii PS1 isolated from Seabuckthorn rhizosphere

Dolkar, Diskit / Phuntsog Dolkar / Stanzin Angmo / O.P. Chaurasia / Tsering Stobdan

Biocatalysis and agricultural biotechnology. 2018 Apr., v. 14

2018

Abstract: Plant growth promotion by microbial inoculants is affected by environmental factors. The study was therefore aimed at developing microbial inoculants for promoting tomato growth in regions experiencing temperature, pH and salt stressed conditions. ... ...

Abstract	Plant growth promotion by microbial inoculants is affected by environmental factors. The study was therefore aimed at developing microbial inoculants for promoting tomato growth in regions experiencing temperature, pH and salt stressed conditions. Enterobacter ludwigii PS1 capable of solubilizing insoluble inorganic phosphate was isolated from Seabuckthorn rhizosphere growing in the Indian trans-Himalaya. PS1 showed ability to solubilize insoluble phosphate under different stress conditions viz. 4–44 °C temperature, 1–5% salt concentration and 4–12 pH range. The isolate exhibited multiple plant growth promoting traits viz. auxin (24.3 mg L−1), siderophore (79%) and hydrogen cyanide (0.21 O.D at 625 nm) production. Tomato seed bacterization resulted in 25% and 37% increase in shoot and root length. Inoculation of tomato seedling with PS1 promoted plant growth in pot trial experiments in trans-Himalayan (34°08′0.2″ N, 77°34′0.3″ E, 3340 m asl) condition. Increase in fruit yield was 15% in open and 27% in shade net condition. E. ludwigii PS1 with phosphate-solubilizing ability under stress conditions appears to be attractive for exploring its plant growth-promoting activities towards development of microbial inoculants in stressed regions.
Keywords	Enterobacter ; Hippophae rhamnoides ; altitude ; auxins ; fruit yield ; growth promotion ; hydrogen cyanide ; pH ; phosphates ; plant growth ; rhizosphere ; roots ; salt concentration ; salt stress ; seedlings ; shoots ; siderophores ; solubilization ; stress tolerance ; temperature ; tomatoes
Language	English
Dates of publication	2018-04
Size	p. 438-443.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2642052-1
ISSN	1878-8181
ISSN	1878-8181
DOI	10.1016/j.bcab.2018.04.012
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Yield optimization, microbial load analysis, and sensory evaluation of mungbean (Vigna radiata L.), lentil (Lens culinaris subsp. culinaris), and Indian mustard (Brassica juncea L.) microgreens grown under greenhouse conditions

Priti / Seema Sangwan / Bharti Kukreja / Gyan Prakash Mishra / Harsh Kumar Dikshit / Ajeet Singh / Muraleedhar Aski / Atul Kumar / Yashpal Taak / Tsering Stobdan / Shouvik Das / Ranjeet R. Kumar / Devendra Kumar Yadava / Shelly Praveen / Shiv Kumar / Ramakrishnan M. Nair

PLoS ONE, Vol 17, Iss

2022 Volume 5

Abstract: Microgreens have been used for raw consumption and are generally viewed as healthy food. This study aimed to optimize the yield parameters, shelf life, sensory evaluation and characterization of total aerobic bacteria (TAB), yeast and mold (Y&M), ... ...

Abstract	Microgreens have been used for raw consumption and are generally viewed as healthy food. This study aimed to optimize the yield parameters, shelf life, sensory evaluation and characterization of total aerobic bacteria (TAB), yeast and mold (Y&M), Escherichia coli, Salmonella spp., and Listeria spp. incidence in mungbean (Vigna radiata (L.) Wilczek), lentil (Lens culinaris Medikus subsp. culinaris), and Indian mustard (Brassica juncea (L.) Czern & Coss.) microgreens. In mungbean and lentil, seeding-density of three seed/cm2, while in Indian mustard, eight seed/cm2 were recorded as optimum. The optimal time to harvest mungbean, Indian mustard, and lentil microgreens were found as 7th, 8th, and 9th day after sowing, respectively. Interestingly, seed size was found highly correlated with the overall yield in both mungbeans (r2 = .73) and lentils (r2 = .78), whereas no such relationship has been recorded for Indian mustard microgreens. The target pathogenic bacteria such as Salmonella spp. and Listeria spp. were not detected; while TAB, Y&M, Shigella spp., and E. coli were recorded well within the limit to cause any human illness in the studied microgreens. Washing with double distilled water for two minutes has shown some reduction in the overall microbial load of these microgreens. The results provided evidence that microgreens if grown and stored properly, are generally safe for human consumption. This is the first study from India on the safety of mungbean, lentils, and Indian mustard microgreens.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Yield optimization, microbial load analysis, and sensory evaluation of mungbean (Vigna radiata L.), lentil (Lens culinaris subsp. culinaris), and Indian mustard (Brassica juncea L.) microgreens grown under greenhouse conditions.

Priti / Seema Sangwan / Bharti Kukreja / Gyan Prakash Mishra / Harsh Kumar Dikshit / Ajeet Singh / Muraleedhar Aski / Atul Kumar / Yashpal Taak / Tsering Stobdan / Shouvik Das / Ranjeet R Kumar / Devendra Kumar Yadava / Shelly Praveen / Shiv Kumar / Ramakrishnan M Nair

PLoS ONE, Vol 17, Iss 5, p e

2022 Volume 0268085

Abstract	Microgreens have been used for raw consumption and are generally viewed as healthy food. This study aimed to optimize the yield parameters, shelf life, sensory evaluation and characterization of total aerobic bacteria (TAB), yeast and mold (Y&M), Escherichia coli, Salmonella spp., and Listeria spp. incidence in mungbean (Vigna radiata (L.) Wilczek), lentil (Lens culinaris Medikus subsp. culinaris), and Indian mustard (Brassica juncea (L.) Czern & Coss.) microgreens. In mungbean and lentil, seeding-density of three seed/cm2, while in Indian mustard, eight seed/cm2 were recorded as optimum. The optimal time to harvest mungbean, Indian mustard, and lentil microgreens were found as 7th, 8th, and 9th day after sowing, respectively. Interestingly, seed size was found highly correlated with the overall yield in both mungbeans (r2 = .73) and lentils (r2 = .78), whereas no such relationship has been recorded for Indian mustard microgreens. The target pathogenic bacteria such as Salmonella spp. and Listeria spp. were not detected; while TAB, Y&M, Shigella spp., and E. coli were recorded well within the limit to cause any human illness in the studied microgreens. Washing with double distilled water for two minutes has shown some reduction in the overall microbial load of these microgreens. The results provided evidence that microgreens if grown and stored properly, are generally safe for human consumption. This is the first study from India on the safety of mungbean, lentils, and Indian mustard microgreens.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: High fat diet induces sex-specific differential gene expression in Drosophila melanogaster.

Tsering Stobdan / Debashis Sahoo / Priti Azad / Iain Hartley / Erilynn Heinrichsen / Dan Zhou / Gabriel G Haddad

PLoS ONE, Vol 14, Iss 3, p e

2019 Volume 0213474

Abstract: Currently about 2 billion adults globally are estimated to be overweight and ~13% of them are obese. High fat diet (HFD) is one of the major contributing factor to obesity, heart disease, diabetes and cancer. Recent findings on the role of HFD in ... ...

Abstract	Currently about 2 billion adults globally are estimated to be overweight and ~13% of them are obese. High fat diet (HFD) is one of the major contributing factor to obesity, heart disease, diabetes and cancer. Recent findings on the role of HFD in inducing abnormalities in neurocognition and susceptibility to Alzheimer's disease are highly intriguing. Since fundamental molecular pathways are often conserved across species, studies involving Drosophila melanogaster as a model organism can provide insight into the molecular mechanisms involving human disease. In order to study some of such mechanisms in the central nervous system as well in the rest of the body, we investigated the effect of HFD on the transcriptome in the heads and bodies of male and female flies kept on either HFD or regular diet (RD). Using comprehensive genomic analyses which include high-throughput transcriptome sequencing, pathway enrichment and gene network analyses, we found that HFD induces a number of responses that are sexually dimorphic in nature. There was a robust transcriptional response consisting of a downregulation of stress-related genes in the heads and glycoside hydrolase activity genes in the bodies of males. In the females, the HFD led to an increased transcriptional change in lipid metabolism. A strong correlation also existed between the takeout gene and hyperphagic behavior in both males and females. We conclude that a) HFD induces a differential transcriptional response between males and females, in heads and bodies and b) the non-dimorphic transcriptional response that we identified was associated with hyperphagia. Therefore, our data on the transcriptional responses in flies to HFD provides potentially relevant information to human conditions including obesity.
Keywords	Medicine ; R ; Science ; Q
Subject code	590
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Apricots (Prunus armeniaca L.) of trans-Himalayan Ladakh: Potential candidate for fruit quality breeding programs

Angmo, Phunchok / Bhuvnesh Kumar / Konchok Targais / Shiv S. Upadhyay / Stanzin Angmo / Tsering Stobdan

Scientia horticulturae. 2017 Apr. 14, v. 218

2017

Abstract: Sixty-five apricot genotypes of trans-Himalayan Ladakh region were studied for phenological and pomological characteristics. Late and extended flowering and fruit maturity was observed in the genotypes. The genotypes attain fruit maturity between August ... ...

Abstract	Sixty-five apricot genotypes of trans-Himalayan Ladakh region were studied for phenological and pomological characteristics. Late and extended flowering and fruit maturity was observed in the genotypes. The genotypes attain fruit maturity between August and early September. Total soluble solids (TSS) ranged from 11.0–37.9°Brix. To the best of our knowledge 37.9°Brix is the highest value reported till date in fresh apricots. The mean Brix:acid ratio was 31.1±17.7. Genotypes with white seed coat are unique to Ladakh, and are associated with sweet kernel, brightly coloured fruit with high TSS. The unique characteristics and variations observed in apricots of Ladakh region, such as late and extended flowering and fruit maturity, high TSS content, white seed coat phenotype, and brightly coloured fruit offer opportunity for exploring the native genotypes for fruit quality improvement programs worldwide.
Keywords	apricots ; breeding programs ; color ; flowering ; fruit maturity ; fruit quality ; genotype ; phenology ; phenotype ; Prunus armeniaca ; seed coat ; seeds ; total soluble solids
Language	English
Dates of publication	2017-0414
Size	p. 187-192.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2017.02.032
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2810: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Ecofriendly Fruit Switches: Graphene Oxide-Based Wrapper for Programmed Fruit Preservative Delivery To Extend Shelf Life

Sharma, Sandeep / Badal Kumar Biswal / Divya kumari / Pulkit Bindra / Satish Kumar / Tsering Stobdan / Vijayakumar Shanmugam

ACS applied materials & interfaces. 2018 May 03, v. 10, no. 22

2018

Abstract: According to Food and Agriculture Organization 2015 report, post-harvest agricultural loss accounts for 20–50% annually; on the other hand, reports about preservatives toxicity are also increasing. Hence, preservative release with response to fruit ... ...

Abstract	According to Food and Agriculture Organization 2015 report, post-harvest agricultural loss accounts for 20–50% annually; on the other hand, reports about preservatives toxicity are also increasing. Hence, preservative release with response to fruit requirement is desired. In this study, acid synthesized in the overripe fruits was envisaged to cleave acid labile hydrazone to release preservative salicylaldehyde from graphene oxide (GO). To maximize loading and to overcome the challenge of GO reduction by hydrazine, two-step activation with ethylenediamine and 4-nitrophenyl chloroformate respectively, are followed. The final composite shows efficient preservative release with the stimuli of the overripe fruit juice and improves the fruit shelf life. The composite shows less toxicity as compared to the free preservative along with the additional scope to reuse. The composite was vacuum-filtered through a 0.4 μm filter paper, to prepare a robust wrapper for the fruit storage.
Keywords	food preservatives ; food-packaging materials ; fruit juices ; fruits ; graphene ; graphene oxide ; hydrazine ; hydrazones ; hydroxybenzaldehyde ; shelf life ; toxicity
Language	English
Dates of publication	2018-0503
Size	p. 18478-18488.
Publishing place	American Chemical Society
Document type	Article
ISSN	1944-8252
DOI	10.1021/acsami.8b02048
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED