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  1. Article ; Online: Role of microbiota-derived corisin in coagulation activation during SARS-CoV-2 infection.

    Tsuruga, Tatsuki / Fujimoto, Hajime / Yasuma, Taro / D'Alessandro-Gabazza, Corina N / Toda, Masaaki / Ito, Toshiyuki / Tomaru, Atsushi / Saiki, Haruko / Okano, Tomohito / Alhawsawi, Manal A B / Takeshita, Atsuro / Nishihama, Kota / Takei, Reoto / Kondoh, Yasuhiro / Cann, Isaac / Gabazza, Esteban C / Kobayashi, Tetsu

    Journal of thrombosis and haemostasis : JTH

    2024  

    Abstract: Background: Coagulopathy is a major cause of morbidity and mortality in COVID-19 patients. Hypercoagulability in COVID-19 results in deep vein thrombosis, thromboembolic complications, and diffuse intravascular coagulation. Microbiome dysbiosis ... ...

    Abstract Background: Coagulopathy is a major cause of morbidity and mortality in COVID-19 patients. Hypercoagulability in COVID-19 results in deep vein thrombosis, thromboembolic complications, and diffuse intravascular coagulation. Microbiome dysbiosis influences the clinical course of COVID-19. However, the role of dysbiosis in COVID-19-associated coagulopathy is not fully understood.
    Objectives: The present study tested the hypothesis that the microbiota-derived proapoptotic corisin is involved in the coagulation system activation during SARS-CoV-2 infection.
    Methods: This cross-sectional study included 47 consecutive patients who consulted for symptoms of COVID-19. A mouse acute lung injury model was used to recapitulate the clinical findings. A549 alveolar epithelial, THP-1, and human umbilical vein endothelial cells were used to evaluate procoagulant and anticoagulant activity of corisin.
    Results: COVID-19 patients showed significantly high circulating levels of corisin, thrombin-antithrombin complex, D-dimer, tumor necrosis factor-α, and monocyte-chemoattractant protein-1 with reduced levels of free protein S compared with healthy subjects. The levels of thrombin-antithrombin complex, D-dimer, and corisin were significantly correlated. A monoclonal anticorisin-neutralizing antibody significantly inhibited the inflammatory response and coagulation system activation in a SARS-CoV-2 spike protein-associated acute lung injury mouse model, and the levels of corisin and thrombin-antithrombin complex were significantly correlated. In an in vitro experiment, corisin increased the tissue factor activity and decreased the anticoagulant activity of thrombomodulin in epithelial, endothelial, and monocytic cells.
    Conclusion: The microbiota-derived corisin is significantly increased and correlated with activation of the coagulation system during SARS-CoV-2 infection, and corisin may directly increase the procoagulant activity in epithelial, endothelial, and monocytic cells.
    Language English
    Publishing date 2024-03-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 2112661-6
    ISSN 1538-7836 ; 1538-7933
    ISSN (online) 1538-7836
    ISSN 1538-7933
    DOI 10.1016/j.jtha.2024.02.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Amelioration of Pulmonary Fibrosis by Matrix Metalloproteinase-2 Overexpression.

    Inoue, Ryo / Yasuma, Taro / Fridman D'Alessandro, Valeria / Toda, Masaaki / Ito, Toshiyuki / Tomaru, Atsushi / D'Alessandro-Gabazza, Corina N / Tsuruga, Tatsuki / Okano, Tomohito / Takeshita, Atsuro / Nishihama, Kota / Fujimoto, Hajime / Kobayashi, Tetsu / Gabazza, Esteban C

    International journal of molecular sciences

    2023  Volume 24, Issue 7

    Abstract: Idiopathic pulmonary fibrosis is a progressive and fatal disease with a poor prognosis. Matrix metalloproteinase-2 is involved in the pathogenesis of organ fibrosis. The role of matrix metalloproteinase-2 in lung fibrosis is unclear. This study evaluated ...

    Abstract Idiopathic pulmonary fibrosis is a progressive and fatal disease with a poor prognosis. Matrix metalloproteinase-2 is involved in the pathogenesis of organ fibrosis. The role of matrix metalloproteinase-2 in lung fibrosis is unclear. This study evaluated whether overexpression of matrix metalloproteinase-2 affects the development of pulmonary fibrosis. Lung fibrosis was induced by bleomycin in wild-type mice and transgenic mice overexpressing human matrix metalloproteinase-2. Mice expressing human matrix metalloproteinase-2 showed significantly decreased infiltration of inflammatory cells and inflammatory and fibrotic cytokines in the lungs compared to wild-type mice after induction of lung injury and fibrosis with bleomycin. The computed tomography score, Ashcroft score of fibrosis, and lung collagen deposition were significantly reduced in human matrix metalloproteinase transgenic mice compared to wild-type mice. The expression of anti-apoptotic genes was significantly increased, while caspase-3 activity was significantly reduced in the lungs of matrix metalloproteinase-2 transgenic mice compared to wild-type mice. Active matrix metalloproteinase-2 significantly decreased bleomycin-induced apoptosis in alveolar epithelial cells. Matrix metalloproteinase-2 appears to protect against pulmonary fibrosis by inhibiting apoptosis of lung epithelial cells.
    MeSH term(s) Mice ; Humans ; Animals ; Matrix Metalloproteinase 2/genetics ; Matrix Metalloproteinase 2/metabolism ; Lung/pathology ; Idiopathic Pulmonary Fibrosis/metabolism ; Bleomycin/adverse effects ; Mice, Transgenic ; Fibrosis ; Mice, Inbred C57BL
    Chemical Substances Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Bleomycin (11056-06-7)
    Language English
    Publishing date 2023-04-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24076695
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Close-to-lesion transbronchial biopsy: a novel technique to improve suitability of specimens for genetic testing in patients with peripheral pulmonary lesions.

    Nishii, Yoichi / Sakaguchi, Tadashi / Esumi, Seiya / Esumi, Maki / Nakamura, Yuki / Suzuki, Yuta / Ito, Kentaro / Fujiwara, Kentaro / Yasui, Hiroki / Ito, Atsushi / Tarukawa, Tomohito / Tsuruga, Tatsuki / D'Alessandro-Gabazza, Corina N / Yasuma, Taro / Fujimoto, Hajime / Asano, Fumihiro / Gabazza, Esteban C / Kobayashi, Tetsu / Taguchi, Osamu /
    Hataji, Osamu

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 14724

    Abstract: Bronchoscopy with radial-probe endobronchial ultrasound, a guide sheath, and electromagnetic navigation can improve the diagnostic yield of peripheral lung nodules. However, the suitability of specimens for genetic analysis remains unsatisfactory. We ... ...

    Abstract Bronchoscopy with radial-probe endobronchial ultrasound, a guide sheath, and electromagnetic navigation can improve the diagnostic yield of peripheral lung nodules. However, the suitability of specimens for genetic analysis remains unsatisfactory. We hypothesized that a transbronchial biopsy performed after closely approaching the bronchoscope tip to the lesion might provide more suitable specimens for genetic analysis. We enrolled 155 patients with peripheral pulmonary lesions who underwent bronchoscopy with a thin or ultrathin bronchoscope. Bronchoscopy was performed using virtual bronchoscopic navigation and radial-probe endobronchial ultrasound with a guide sheath. The bronchoscope tip was placed closer to the lesion during bronchoscopy to collect larger specimens with higher malignant cell content. The patients who underwent a close-to-lesion biopsy had higher rates of overall diagnostic yield, histopathological diagnostic yield, and specimen quality for genetic testing than those who did not. The significant determinants of the specimen's suitability were the close-to-lesion approach, within-the-lesion image, the use of standard 1.9-mm-forceps, and the number of cancer-cell-positive specimens. The significant predictors of the specimen's suitability for genetic analysis were close-to-lesion biopsy and the number of malignant cell-positive tissue samples. This study demonstrates that the close-to-lesion transbronchial biopsy significantly improves the suitability of bronchoscopic specimens for genetic analysis.
    MeSH term(s) Humans ; Male ; Genetic Testing ; Bronchoscopy ; Biopsy ; Endosonography ; Foreskin
    Language English
    Publishing date 2023-09-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-41726-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A Microbiome-Derived Peptide Induces Apoptosis of Cells from Different Tissues.

    Saiki, Haruko / Okano, Yuko / Yasuma, Taro / Toda, Masaaki / Takeshita, Atsuro / Abdel-Hamid, Ahmed M / Fridman D'Alessandro, Valeria / Tsuruga, Tatsuki / D'Alessandro-Gabazza, Corina N / Katayama, Kan / Sugimoto, Masahiko / Fujimoto, Hajime / Yamanaka, Keiichi / Kobayashi, Tetsu / Cann, Isaac / Gabazza, Esteban C

    Cells

    2021  Volume 10, Issue 11

    Abstract: Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may ... ...

    Abstract Apoptosis is a programmed cell death involved in embryogenesis and tissue homeostasis under physiological conditions. However, abnormalities in the process of apoptosis are implicated in the pathogenesis of various diseases. The human microbiota may release products that induce apoptosis of host cells. We recently identified a novel microbiome-derived peptide called corisin that worsens lung fibrosis by inducing apoptosis of lung epithelial cells. We hypothesized that corisin and a corisin-like peptide might also induce apoptosis of cells from different tissues. We cultured podocytes, renal tubular epithelial cells, keratinocytes, retinal and intestinal cells treated with corisin and evaluated apoptosis by flow cytometry and Western blotting. Although at different grades, flow cytometry analysis and Western blotting showed that corisin and a corisin-like peptide induced apoptosis of podocytes, keratinocytes, tubular epithelial cells, retinal, and intestinal cells. In addition, we found that corisin synergistically enhances the proapoptotic activity of transforming growth factor-β1 on podocytes. In conclusion, these results suggest that corisin and corisin-like peptides may play a role in the pathogenesis of disease in different organs by promoting apoptosis of parenchymal cells.
    MeSH term(s) Apoptosis/drug effects ; Caspase 3/metabolism ; Cell Line, Tumor ; Epithelial Cells/drug effects ; Epithelial Cells/pathology ; HaCaT Cells ; Humans ; Keratinocytes/drug effects ; Keratinocytes/pathology ; Microbiota/drug effects ; Mitochondrial Membranes/drug effects ; Mitochondrial Membranes/metabolism ; Organ Specificity/drug effects ; Peptides/pharmacology ; Podocytes/drug effects ; Podocytes/pathology ; Reactive Oxygen Species/metabolism ; Retina/pathology ; Transforming Growth Factor beta1/metabolism
    Chemical Substances Peptides ; Reactive Oxygen Species ; Transforming Growth Factor beta1 ; Caspase 3 (EC 3.4.22.-)
    Language English
    Publishing date 2021-10-26
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10112885
    Database MEDical Literature Analysis and Retrieval System OnLINE

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