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  1. Article: Laminin 521 Modulates the Сytotoxic Effect of 5-Fluorouracil on HT29 Colorectal Cancer Cells

    Raigorodskaya, M. P. / Turchinovich, A. / Tsypina, I. M. / Zgoda, V. G. / Nikulin, S. V. / Maltseva, D. V.

    Applied biochemistry and microbiology. 2020 Dec., v. 56, no. 8

    2020  

    Abstract: The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF), drugs with different mechanisms of action used to treat colorectal cancer, on an HT29 cell line cultured on plastic or laminin 521 (LM-521) has been studied. It is first shown that LM-521 ...

    Abstract The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF), drugs with different mechanisms of action used to treat colorectal cancer, on an HT29 cell line cultured on plastic or laminin 521 (LM-521) has been studied. It is first shown that LM-521 can increase the sensitivity of tumor cells to 5FU. A possible mechanism of the observed effect of LM-521 on the HT29 cell viability is proposed based on transcriptome and proteome analysis. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways and promote phosphorylation of the YAP transcription coactivator and its binding to the complex with the 14-3-3σ protein. The formation of this complex leads to YAP retention in the cytoplasm and prevents its transport to the nucleus and the activation of antiapoptotic gene transcription.
    Keywords cell lines ; cell viability ; colorectal neoplasms ; cytoplasm ; cytotoxicity ; fluorouracil ; integrins ; laminin ; microbiology ; phosphorylation ; proteome ; transcription (genetics) ; transcriptome
    Language English
    Dates of publication 2020-12
    Size p. 870-874.
    Publishing place Pleiades Publishing
    Document type Article
    ZDB-ID 412550-2
    ISSN 1608-3024 ; 0003-6838
    ISSN (online) 1608-3024
    ISSN 0003-6838
    DOI 10.1134/S0003683820080074
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  2. Article: [Epithelial to Mesenchymal Transition Marker in 2D and 3D Colon Cancer Cell Cultures in the Presence of Laminin 332 and 411].

    Maltseva, D V / Makarova, J A / Khristichenko, A Yu / Tsypina, I M / Tonevitsky, E A / Rodin, S A

    Molekuliarnaia biologiia

    2019  Volume 53, Issue 2, Page(s) 330–338

    Abstract: Received August 28, 2018; revised October 10, 2018; accepted November 6, 2018 The loss of apical-basal cell polarity is a necessary stage of the epithelial-mesenchymal transition (EMT). Polarized epithelial cells interact with the basement membrane (BM) ... ...

    Abstract Received August 28, 2018; revised October 10, 2018; accepted November 6, 2018 The loss of apical-basal cell polarity is a necessary stage of the epithelial-mesenchymal transition (EMT). Polarized epithelial cells interact with the basement membrane (BM) and, in particular, with laminins, the major components of BM. Here, we examined the effect of the transition of colon cancer cells from 2D polarized state to non-polarized 3D state on the expression of EMT associated genes, as well as the role of laminins 332 and 411 (LM-332 and LM-411) in this process. The three studied cell lines, HT-29, HCT-116 and RKO, were found to have different sensitivity to cultivation conditions (2D to 3D changes) and to addition of laminins. One of the possible reasons for this maybe a difference in the initial 2D state of the cells. In particular, it was shown that the cell lines were at different EMT stages. HT-29 exhibited more epithelial expression profile, RKO was more mesenchymal, and HCT-116 was in an intermediate state. The most laminin-sensitive cell line was HCT-116. The magnitude and the specificity of cell response to LM-332 and LM-411 depended on the expression pattern of laminins' receptors. EMT gene expression profile was not substantially changed neither during the transition from 2D to 3D state, nor the presence of laminins' isoforms. However, we detected changes in expression of SNAI1 and ZEB1 genes encoding transcription factors that control the EMT process. Notably, in all three studied cell lines, the expression of SNAI1 was enhanced in response to laminin treatment.
    MeSH term(s) Biomarkers, Tumor/metabolism ; Cell Adhesion Molecules/pharmacology ; Cell Culture Techniques/methods ; Cell Line, Tumor ; Colonic Neoplasms/metabolism ; Colonic Neoplasms/pathology ; Epithelial-Mesenchymal Transition/drug effects ; Humans ; Laminin/pharmacology ; Kalinin
    Chemical Substances Biomarkers, Tumor ; Cell Adhesion Molecules ; Laminin
    Language Russian
    Publishing date 2019-05-16
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 213542-5
    ISSN 0026-8984
    ISSN 0026-8984
    DOI 10.1134/S0026898419020113
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1796: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  3. Article ; Online: Oxyquinoline-Dependent Changes in Claudin-Encoding Genes Contribute to Impairment of the Barrier Function of the Trophoblast Monolayer.

    Knyazev, E N / Petrov, V A / Gazizov, I N / Gerasimenko, T N / Tsypina, I M / Tonevitsky, A G / Sukhikh, G T

    Bulletin of experimental biology and medicine

    2019  Volume 166, Issue 3, Page(s) 369–372

    Abstract: Natural response to hypoxia critically depends on rapid stabilization of hypoxia-inducible factor (HIF). Under normoxic conditions, HIF-prolyl hydroxylases mark α-subunits of HIF for degradation, while hypoxia results in stabilization of HIF-α. ... ...

    Abstract Natural response to hypoxia critically depends on rapid stabilization of hypoxia-inducible factor (HIF). Under normoxic conditions, HIF-prolyl hydroxylases mark α-subunits of HIF for degradation, while hypoxia results in stabilization of HIF-α. Oxyquinoline derivatives suppress activity of HIF-prolyl hydroxylases leading to HIF activation in the cell. Here we show that 24-h incubation of BeWo b30 choriocarcinoma cells (a model of trophoblast in the placental barrier) with oxyquinoline derivative leads to a decrease in transepithelial electrical resistance (TEER) of the cell monolayer, while the permeability of the monolayer for FITC-dextran (70 kDa) remains unchanged. These findings suggest that the overall barrier function is preserved, while the structure of intercellular tight junctions can undergo minor changes. Using Affymetrix Human Transcriptome Array 2.0, we showed that the treatment with oxyquinoline derivative was followed by a decrease in the expression of claudins 6 and 7 (CLDN6, CLDN7), occludin (OCLN), contact adhesion molecule 3 (JAM3), and angiomotinlike protein 1 (AMOTL1).
    MeSH term(s) Cell Adhesion Molecules/genetics ; Cell Adhesion Molecules/metabolism ; Cell Hypoxia/genetics ; Cell Line, Tumor ; Claudins/genetics ; Claudins/metabolism ; Dextrans/metabolism ; Electric Impedance ; Female ; Fluorescein-5-isothiocyanate/analogs & derivatives ; Fluorescein-5-isothiocyanate/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Models, Biological ; Occludin/genetics ; Occludin/metabolism ; Oxyquinoline/pharmacology ; Permeability/drug effects ; Pre-Eclampsia/genetics ; Pre-Eclampsia/metabolism ; Pre-Eclampsia/pathology ; Pregnancy ; Tight Junctions/drug effects ; Tight Junctions/genetics ; Tight Junctions/metabolism ; Trophoblasts/drug effects ; Trophoblasts/metabolism ; Trophoblasts/pathology
    Chemical Substances AMOTL1 protein, human ; CLDN7 protein, human ; Cell Adhesion Molecules ; Claudins ; Dextrans ; JAM3 protein, human ; Membrane Proteins ; OCLN protein, human ; Occludin ; fluorescein isothiocyanate dextran ; Oxyquinoline (5UTX5635HP) ; Fluorescein-5-isothiocyanate (I223NX31W9) ; claudin 6 (MRC5FX426I)
    Language English
    Publishing date 2019-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390407-6
    ISSN 1573-8221 ; 0007-4888 ; 0365-9615
    ISSN (online) 1573-8221
    ISSN 0007-4888 ; 0365-9615
    DOI 10.1007/s10517-019-04352-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 56: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Metabolic Reprogramming of Trophoblast Cells in Response to Hypoxia.

    Knyazev, E N / Zakharova, G S / Astakhova, L A / Tsypina, I M / Tonevitsky, A G / Sukhikh, G T

    Bulletin of experimental biology and medicine

    2019  Volume 166, Issue 3, Page(s) 321–325

    Abstract: Hypoxia of trophoblast cells is an important regulator of normal development of the placenta. However, some pathological states associated with hypoxia, e.g. preeclampsia, impair the functions of placental cells. Oxyquinoline derivative inhibits HIF- ... ...

    Abstract Hypoxia of trophoblast cells is an important regulator of normal development of the placenta. However, some pathological states associated with hypoxia, e.g. preeclampsia, impair the functions of placental cells. Oxyquinoline derivative inhibits HIF-prolyl hydroxylase by stabilizing HIF-1 transcription complex, thus modeling cell response to hypoxia. In human choriocarcinoma cells BeWo b30 (trophoblast model), oxyquinoline increased the expression of a core hypoxia response genes along with up-regulation of NOS3, PDK1, and BNIP3 genes and down-regulation of the PPARGC1B gene. These changes in the expression profile attest to activation of the metabolic cell reprogramming mechanisms aimed at reducing oxygen consumption by enabling the switch from aerobic to anaerobic glucose metabolism and the respective decrease in number of mitochondria. The possibility of practical use of the therapeutic properties of oxyquinoline derivatives is discussed.
    MeSH term(s) Carrier Proteins/antagonists & inhibitors ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cell Hypoxia/genetics ; Cell Line, Tumor ; Cellular Reprogramming ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Glycolysis/drug effects ; Glycolysis/genetics ; Humans ; Membrane Proteins/agonists ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Models, Biological ; Nitric Oxide Synthase Type III/genetics ; Nitric Oxide Synthase Type III/metabolism ; Oxidative Phosphorylation/drug effects ; Oxyquinoline/pharmacology ; Pre-Eclampsia/genetics ; Pre-Eclampsia/metabolism ; Pre-Eclampsia/pathology ; Pregnancy ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; Proto-Oncogene Proteins/agonists ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/metabolism ; Trophoblasts/drug effects ; Trophoblasts/metabolism ; Trophoblasts/pathology
    Chemical Substances BNIP3 protein, human ; Carrier Proteins ; Membrane Proteins ; PPARGC1B protein, human ; Proto-Oncogene Proteins ; Oxyquinoline (5UTX5635HP) ; NOS3 protein, human (EC 1.14.13.39) ; Nitric Oxide Synthase Type III (EC 1.14.13.39) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; pyruvate dehydrogenase (acetyl-transferring) kinase (EC 2.7.11.2)
    Language English
    Publishing date 2019-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390407-6
    ISSN 1573-8221 ; 0007-4888 ; 0365-9615
    ISSN (online) 1573-8221
    ISSN 0007-4888 ; 0365-9615
    DOI 10.1007/s10517-019-04342-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 56: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Opyt organizatsii sanitarnogo nadzora za kooperativnoĭ deiatel'nost'iu.

    Smirnova, O E / Tsypina, I M / Chebotareva, M Iu

    Sovetskoe zdravookhranenie

    1989  , Issue 4, Page(s) 45–47

    Title translation Experience with the organization of sanitary control of cooperative activities.
    MeSH term(s) Commission on Professional and Hospital Activities/organization & administration ; Facility Regulation and Control/standards ; Interprofessional Relations ; Medical Audit/organization & administration ; Moscow ; Occupational Medicine/standards ; Public Health/standards ; United States
    Language Russian
    Publishing date 1989
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 128566-x
    ISSN 0038-5239
    ISSN 0038-5239
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 970: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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