Article: Degradation of nuclear Ubc9 induced by listeriolysin O is dependent on K+ efflux
Biochemical and biophysical research communications. 2017,
2017
Abstract: Listeriolysin O (LLO) is a pore-forming toxin produced by L. monocytogenes, and is belonged to a protein family of cholesterol-dependent cytolysins (CDCs). Previous studies have demonstrated that LLO triggers Ubc9 degradation and disrupts host ... ...
| Abstract | Listeriolysin O (LLO) is a pore-forming toxin produced by L. monocytogenes, and is belonged to a protein family of cholesterol-dependent cytolysins (CDCs). Previous studies have demonstrated that LLO triggers Ubc9 degradation and disrupts host SUMOylation to facilitate bacterial infection. However, the underlying mechanism of Ubc9 degradation is unclear. Here we show that LLO-induced down-regulation of Ubc9 is independent of Ubc9-SUMO interaction, however, it may involve phosphorylation signaling. Additionally, LLO exerts its effects primarily on nuclear Ubc9 and this process is mediated by K+ efflux. Interestingly, for intracellular CDCs such as pneumolysin and suilysin, blockage of K+ efflux enhances degradation of nuclear Ubc9, suggesting that extracellular and intracellular pathogens may exploit different mechanisms to modulate host SUMOylation system. Furthermore, up-regulation of SUMOylation by stable expression of SUMO-1 or SUMO-2 shows a delay in membrane perforation by LLO, indicating that SUMO modification of host proteins may act at the frontline for the defense response against LLO. Taken together, our study provides insights to the understanding of host-pathogen interactions. |
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| Keywords | bacterial infections ; host-pathogen relationships ; pathogens ; phosphorylation ; pneumolysin ; potassium ; proteins | ||||||||||
| Language | English | ||||||||||
| Size | p. . | ||||||||||
| Publishing place | Elsevier Inc. | ||||||||||
| Document type | Article | ||||||||||
| Note | Pre-press version | ||||||||||
| ZDB-ID | 205723-2 | ||||||||||
| ISSN | 0006-291X ; 0006-291X | ||||||||||
| ISSN (online) | 0006-291X | ||||||||||
| ISSN | 0006-291X | ||||||||||
| DOI | 10.1016/j.bbrc.2017.09.051 | ||||||||||
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| Database | NAL-Catalogue (AGRICOLA) |
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