Article: The leucine regulon of Escherichia coli K-12: a mutation in rblA alters expression of L-leucine-dependent metabolic operons.
1990 Volume 172, Issue 8, Page(s) 4529–4535
Abstract: We have isolated and characterized a highly pleiotropic Escherichia coli mutant affected in the activity of a number of enzymes involved in different metabolic pathways, all of which are regulated by leucine. Selected for its ability to grow with L- ... ...
| Abstract | We have isolated and characterized a highly pleiotropic Escherichia coli mutant affected in the activity of a number of enzymes involved in different metabolic pathways, all of which are regulated by leucine. Selected for its ability to grow with L-serine as sole carbon source, the rbl-1::Tn10 mutant had high levels of L-serine deaminase activity (due to increased transcription of the structural gene) and of another amino acid-degrading enzyme, L-threonine dehydrogenase, and decreased transcription of the operons serA and ilvIH, coding for biosynthetic enzymes. The rbl mutation suppressed the slow growth of a metK mutant, deficient in S-adenosylmethionine synthetase. Furthermore, metK mutants spontaneously accumulated faster-growing rbl-like derivatives, and a commonly used metK strain, RG62, carries such a mutation. The rbl gene is located near 20 min on the E. coli genetic map. All phenotypes of the rbl mutant could be observed in rbl+ strains cultivated in the presence of L-leucine, and exogenous L-leucine had little further effect on the rbl strains. We propose that the rbl gene product is the regulator of a global response to leucine. |
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| MeSH term(s) | Alcohol Oxidoreductases/genetics ; Alcohol Oxidoreductases/metabolism ; DNA Transposable Elements ; Escherichia coli/genetics ; Genes, Regulator ; Genotype ; L-Serine Dehydratase/genetics ; L-Serine Dehydratase/metabolism ; Leucine/metabolism ; Methionine Adenosyltransferase/genetics ; Mutation ; Operon ; Transcription, Genetic ; beta-Galactosidase/genetics ; beta-Galactosidase/metabolism | |||||
| Chemical Substances | DNA Transposable Elements ; Alcohol Oxidoreductases (EC 1.1.-) ; L-threonine 3-dehydrogenase (EC 1.1.1.103) ; Methionine Adenosyltransferase (EC 2.5.1.6) ; beta-Galactosidase (EC 3.2.1.23) ; L-Serine Dehydratase (EC 4.3.1.17) ; Leucine (GMW67QNF9C) | |||||
| Language | English | |||||
| Publishing date | 1990-08 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 2968-3 | |||||
| ISSN | 1098-5530 ; 0021-9193 | |||||
| ISSN (online) | 1098-5530 | |||||
| ISSN | 0021-9193 | |||||
| DOI | 10.1128/jb.172.8.4529-4535.1990 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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