Article ; Online: ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.
2017 Volume 45, Issue 15, Page(s) 9093–9107
Abstract: Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We ... ...
| Abstract | Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1 and ALG-2, as well as changes in mRNA expression in alg-5, alg-1 and alg-2 mutants. We show that ALG-5 defines a distinct branch of the miRNA pathway affecting the expression of genes involved in immunity, defense, and development. In contrast to ALG-1 and ALG-2, which associate with most miRNAs and have general roles throughout development, ALG-5 interacts with only a small subset of miRNAs and is specifically expressed in the germline where it localizes alongside the piRNA and siRNA machinery at P granules. alg-5 is required for optimal fertility and mutations in alg-5 lead to a precocious transition from spermatogenesis to oogenesis. Our results provide a near-comprehensive analysis of miRNA-Argonaute interactions in C. elegans and reveal a new role for miRNAs in the germline. |
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| MeSH term(s) | Animals ; Argonaute Proteins/genetics ; Argonaute Proteins/metabolism ; Caenorhabditis elegans/classification ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/growth & development ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Gene Expression Regulation, Developmental ; Germ Cells/growth & development ; Germ Cells/metabolism ; Hermaphroditic Organisms/genetics ; Hermaphroditic Organisms/growth & development ; Hermaphroditic Organisms/metabolism ; High-Throughput Nucleotide Sequencing ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Mutation ; Oogenesis/genetics ; Phylogeny ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; RNA, Helminth/genetics ; RNA, Helminth/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Spermatogenesis/genetics |
| Chemical Substances | ALG-1 protein, C elegans ; ALG-2 protein, C elegans ; ALG-5 protein, C elegans ; Argonaute Proteins ; Caenorhabditis elegans Proteins ; MicroRNAs ; Protein Isoforms ; RNA, Helminth ; RNA, Messenger ; RNA, Small Interfering ; RNA-Binding Proteins |
| Language | English |
| Publishing date | 2017-09-06 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 186809-3 |
| ISSN | 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048 |
| ISSN (online) | 1362-4962 ; 1362-4954 |
| ISSN | 0301-5610 ; 0305-1048 |
| DOI | 10.1093/nar/gkx536 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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