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Article: Dedifferentiating germ cells regain stem-cell specific polarity checkpoint prior to niche reentry.

Bener, Muhammed Burak / Twillie, Autumn / Inaba, Mayu

bioRxiv : the preprint server for biology

2023

Abstract: In the Drosophila germline stem cell system, maintenance of the stem cell pool requires "dedifferentiation", in which differentiating cells reattach to the niche and reacquire stem cell properties. However, the mechanism of dedifferentiation remains ... ...

Abstract	In the Drosophila germline stem cell system, maintenance of the stem cell pool requires "dedifferentiation", in which differentiating cells reattach to the niche and reacquire stem cell properties. However, the mechanism of dedifferentiation remains poorly understood. Here, using long-term live imaging, we show that dedifferentiated cells immediately re-enter mitosis with correct spindle orientation after reattachment to the niche. Analysis of cell cycle markers revealed that these dedifferentiating cells are all in G2 phase. In addition, we found that the observed G2 block during dedifferentiation likely corresponds to a centrosome orientation checkpoint (COC), a previously reported polarity checkpoint. We show that re-activation of a COC is likely required for the dedifferentiation thus ensuring asymmetric division even in dedifferentiated stem cells. Taken together, our study demonstrates the remarkable ability of dedifferentiating cells to reacquire the ability to divide asymmetrically.
Language	English
Publishing date	2023-04-28
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.04.26.538507
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Diffusible fraction of niche BMP ligand safeguards stem-cell differentiation.

Ridwan, Sharif M / Twillie, Autumn / Poursaeid, Samaneh / Beard, Emma Kristine / Bener, Muhammed Burak / Antel, Matthew / Cowan, Ann E / Matsuda, Shinya / Inaba, Mayu

Nature communications

2024 Volume 15, Issue 1, Page(s) 1166

Abstract: Drosophila male germline stem cells (GSCs) reside at the tip of the testis and surround a cluster of niche cells. Decapentaplegic (Dpp) is one of the well-established ligands and has a major role in maintaining stem cells located in close proximity. ... ...

Abstract	Drosophila male germline stem cells (GSCs) reside at the tip of the testis and surround a cluster of niche cells. Decapentaplegic (Dpp) is one of the well-established ligands and has a major role in maintaining stem cells located in close proximity. However, the existence and the role of the diffusible fraction of Dpp outside of the niche have been unclear. Here, using genetically-encoded nanobodies called Morphotraps, we physically block Dpp diffusion without interfering with niche-stem cell signaling and find that a diffusible fraction of Dpp is required to ensure differentiation of GSC daughter cells, opposite of its role in maintenance of GSC in the niche. Our work provides an example in which a soluble niche ligand induces opposed cellular responses in stem cells versus in differentiating descendants to ensure spatial control of the niche. This may be a common mechanism to regulate tissue homeostasis.
MeSH term(s)	Animals ; Male ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Ligands ; Cell Differentiation/physiology ; Drosophila/metabolism ; Signal Transduction/physiology ; Stem Cell Niche/physiology ; Germ Cells/metabolism ; Drosophila melanogaster/metabolism
Chemical Substances	Drosophila Proteins ; Ligands
Language	English
Publishing date	2024-02-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45408-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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