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Article ; Online: Plasma levels of arginines at term pregnancy in relation to mode of onset of labor and mode of childbirth.

Akhter, Tansim / Hedeland, Mikael / Bergquist, Jonas / Ubhayasekera, Kumari / Larsson, Anders / Kullinger, Merit / Skalkidou, Alkistis

American journal of reproductive immunology (New York, N.Y. : 1989)

2023 Volume 90, Issue 3, Page(s) e13767

Abstract: Problem: The exact biochemical mechanisms that initiate labor are not yet fully understood. Nitric oxide is a potent relaxant of uterine smooth muscles until labor starts, and its precursor is L-arginine. Asymmetric (ADMA) and symmetric (SDMA) ... ...

Abstract	Problem: The exact biochemical mechanisms that initiate labor are not yet fully understood. Nitric oxide is a potent relaxant of uterine smooth muscles until labor starts, and its precursor is L-arginine. Asymmetric (ADMA) and symmetric (SDMA) dimethylarginines, are potent NO-inhibitors. However, arginines (dimethylarginines and L-arginine) are scarcely studied in relation to labor and childbirth. We aimed to investigate arginines in women with spontaneous (SLVB) and induced (ILVB) term labor with vaginal birth and in women undergoing elective caesarean section (ECS). Method of study: Women at gestational week 16-18 were recruited to the population-based prospective cohort study BASIC at the Uppsala University Hospital, Sweden. Plasma samples taken at start of labor were analyzed for arginines, from SLVB (n = 45), ILVB (n = 45), and ECS (n = 45), using Ultra-High Performance Liquid Chromatography. Between-group differences were assessed using Kruskal-Wallis and Mann-Whitney U-test. Results: Women with SLVB and ILVB had higher levels of ADMA (p < .0001), SDMA (p < .05) and lower L-arginines (p < .01), L-arginine/ADMA (p < .0001), and L-arginine/SDMA (p < .01, respectively <.001) compared to ECS. However, ILVB had higher ADMA (p < .0001) and lower L-arginine (p < .01), L-arginine/ADMA (p < .0001), and L-arginine/SDMA (p < .01) compared to SLVB. Results are adjusted for gestational length at birth and cervical dilatation at sampling. Conclusion: Our novel findings of higher levels of dimethylarginines in term vaginal births compared to ECS give insights into the biochemical mechanisms of labor. These findings might also serve as a basis for further studies of arginines in complicated pregnancies and labor.
MeSH term(s)	Pregnancy ; Infant, Newborn ; Female ; Humans ; Cesarean Section ; Prospective Studies ; Parturition ; Delivery, Obstetric ; Arginine
Chemical Substances	Arginine (94ZLA3W45F)
Language	English
Publishing date	2023-08-28
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604542-x
ISSN	1600-0897 ; 0271-7352 ; 8755-8920 ; 1046-7408
ISSN (online)	1600-0897
ISSN	0271-7352 ; 8755-8920 ; 1046-7408
DOI	10.1111/aji.13767
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1653: Show issues

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Article: Hyperandrogenic Symptoms Are a Persistent Suffering in Midlife Women with PCOS; a Prospective Cohort Study in Sweden.

Persson, Sofia / Ubhayasekera, Kumari A / Bergquist, Jonas / Turkmen, Sahruh / Poromaa, Inger Sundström / Elenis, Evangelia

Biomedicines

2022 Volume 11, Issue 1

Abstract: Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women, and the majority suffers from hyperandrogenism. Hyperandrogenism causes psychological morbidity and impaired quality of life in women with PCOS during the reproductive years, ... ...

Abstract	Polycystic ovary syndrome (PCOS) is a common endocrine disorder among women, and the majority suffers from hyperandrogenism. Hyperandrogenism causes psychological morbidity and impaired quality of life in women with PCOS during the reproductive years, but data on prevalence and impact during midlife are lacking. Thus, this study aimed to address whether hyperandrogenism persists into midlife and, if so, what impact it has on quality of life. In order to answer this question, we performed a multicenter prospective cohort study, where we included women already diagnosed with PCOS who had reached the age of 45 years or more and age-matched controls. All participants underwent a physical exam, structured medical interview, biochemical testing and filled out self-assessment questionnaires. More than 40% of the women with PCOS and 82% of those who presented with the hyperandrogenic phenotype at the diagnostic work-up still suffered from hirsutism. Circulating testosterone levels were similar between women with PCOS and controls while free androgen index was higher in women with PCOS, independent of weight. Women with hyperandrogenic PCOS expressed persisting concerns regarding hirsutism at the follow-up assessment. In conclusion, women with PCOS who present with hyperandrogenic symptoms at the time they are diagnosed with PCOS have a higher risk of persistent androgenic symptoms and impaired quality of life in midlife.
Language	English
Publishing date	2022-12-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11010096
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Article ; Online: The association between maternal body mass index and serial plasma oxytocin levels during labor.

Ramö Isgren, Anna / Carlhäll, Sara / Dennis Retrato, Mark / Kodikara, Chamali / A Ubhayasekera, Kumari / Kjölhede, Preben / Bergquist, Jonas / Blomberg, Marie

PloS one

2023 Volume 18, Issue 8, Page(s) e0290038

Abstract: Objective: To evaluate the association between maternal body mass index (BMI) and plasma oxytocin (OT) levels at different OT infusion rates in labor.: Methods: A prospective observational study analyzing serial plasma samples in laboring women with ... ...

Abstract	Objective: To evaluate the association between maternal body mass index (BMI) and plasma oxytocin (OT) levels at different OT infusion rates in labor. Methods: A prospective observational study analyzing serial plasma samples in laboring women with OT infusion. The women were categorized into three groups, women with non-obesity (BMI 18.5-29.9, n = 12), obesity (BMI 30.0-34.9, n = 13), and morbid obesity (BMI ≥ 35.0, n = 15). Plasma OT was analyzed using tandem mass spectrometry. Results: Except for a low positive correlation between OT levels and BMI and significantly increased plasma OT levels in women with morbid obesity at the OT infusion rate of 3.3 mU/min, no significant differences in OT levels between the BMI groups were found. Further, the inter-individual differences in OT levels were large and no dose-dependent increase of OT levels was seen. Conclusions: Other factors than plasma OT levels may be more likely to determine the clinical response of OT infusion in women with obesity. Perhaps the observed clinical need and individual response would be a better predictor of plasma OT levels than a pre-determined OT infusion rate. The OT dosage guidelines for labor augmentation should be individualized according to clinical response rather than generalized. Trial registration: Clinical trial registration: ClinicalTrials.gov ID NCT04093479.
MeSH term(s)	Pregnancy ; Female ; Humans ; Oxytocin ; Body Mass Index ; Obesity, Morbid ; Labor, Obstetric ; Labor, Induced/methods
Chemical Substances	Oxytocin (50-56-6)
Language	English
Publishing date	2023-08-11
Publishing country	United States
Document type	Observational Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0290038
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Article: Endogenous Levels of Gamma Amino-Butyric Acid Are Correlated to Glutamic-Acid Decarboxylase Antibody Levels in Type 1 Diabetes.

Hill, Henrik / Elksnis, Andris / Lundkvist, Per / Ubhayasekera, Kumari / Bergquist, Jonas / Birnir, Bryndis / Carlsson, Per-Ola / Espes, Daniel

Biomedicines

2021 Volume 10, Issue 1

Abstract: Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system (CNS) and outside of the CNS, found in the highest concentrations in immune cells and pancreatic beta-cells. GABA is gaining increasing interest in ... ...

Abstract	Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system (CNS) and outside of the CNS, found in the highest concentrations in immune cells and pancreatic beta-cells. GABA is gaining increasing interest in diabetes research due to its immune-modulatory and beta-cell stimulatory effects and is a highly interesting drug candidate for the treatment of type 1 diabetes (T1D). GABA is synthesized from glutamate by glutamic acid decarboxylase (GAD), one of the targets for autoantibodies linked to T1D. Using mass spectrometry, we have quantified the endogenous circulating levels of GABA in patients with new-onset and long-standing T1D and found that the levels are unaltered when compared to healthy controls, i.e., T1D patients do not have a deficit of systemic GABA levels. In T1D, GABA levels were negatively correlated with IL-1 beta, IL-12, and IL-15 15 and positively correlated to levels of IL-36 beta and IL-37. Interestingly, GABA levels were also correlated to the levels of GAD-autoantibodies. The unaltered levels of GABA in T1D patients suggest that the GABA secretion from beta-cells only has a minor impact on the circulating systemic levels. However, the local levels of GABA could be altered within pancreatic islets in the presence of GAD-autoantibodies.
Language	English
Publishing date	2021-12-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10010091
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Article ; Online: Inclusion of Endogenous Plasma Dehydroepiandrosterone Sulfate and Mammographic Density in Risk Prediction Models for Breast Cancer.

Gabrielson, Marike / Ubhayasekera, Kumari A / Acharya, Santosh R / Franko, Mikael Andersson / Eriksson, Mikael / Bergquist, Jonas / Czene, Kamila / Hall, Per

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology

2020 Volume 29, Issue 3, Page(s) 574–581

Abstract: Background: Endogenous hormones and mammographic density are risk factors for breast cancer. Joint analyses of the two may improve the ability to identify high-risk women.: Methods: This study within the KARMA cohort included prediagnostic measures ... ...

Abstract	Background: Endogenous hormones and mammographic density are risk factors for breast cancer. Joint analyses of the two may improve the ability to identify high-risk women. Methods: This study within the KARMA cohort included prediagnostic measures of plasma hormone levels of dehydroepiandrosterone (DHEA), its sulfate (DHEAS), and mammographic density in 629 cases and 1,223 controls, not using menopausal hormones. We evaluated the area under the receiver-operating curve (AUC) for risk of breast cancer by adding DHEA, DHEAS, and mammographic density to the Gail or Tyrer-Cuzick 5-year risk scores or the CAD2Y 2-year risk score. Results: DHEAS and percentage density were independently and positively associated with breast cancer risk ( Conclusions: DHEAS and mammographic density are independent risk factors for breast cancer and improve risk discrimination for postmenopausal breast cancer. Impact: Combining DHEAS and mammographic density could help identify women at high risk who may benefit from individualized breast cancer screening and/or preventive measures among postmenopausal women.
MeSH term(s)	Adult ; Breast/diagnostic imaging ; Breast Density ; Breast Neoplasms/epidemiology ; Breast Neoplasms/prevention & control ; Case-Control Studies ; Dehydroepiandrosterone Sulfate/blood ; Feasibility Studies ; Female ; Humans ; Incidence ; Mammography ; Middle Aged ; Postmenopause/blood ; Prospective Studies ; ROC Curve ; Risk Assessment/methods ; Risk Factors
Chemical Substances	Dehydroepiandrosterone Sulfate (57B09Q7FJR)
Language	English
Publishing date	2020-01-16
Publishing country	United States
Document type	Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
ZDB-ID	1153420-5
ISSN	1538-7755 ; 1055-9965
ISSN (online)	1538-7755
ISSN	1055-9965
DOI	10.1158/1055-9965.EPI-19-1120
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3693: Show issues

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Article: Effects of glucocorticoids on vitamin D3-metabolizing 24-hydroxylase (CYP24A1) in Saos-2 cells and primary human osteoblasts

Zayny, Ahmad / Almokhtar, Mokhtar / Wikvall, Kjell / Ljunggren, Östen / Ubhayasekera, Kumari / Bergquist, Jonas / Kibar, Pinar / Norlin, Maria

Molecular and cellular endocrinology. 2019 Oct. 01, v. 496

2019

Abstract: Vitamin D is essential for bone function and deficiency in active vitamin D hormone can lead to bone disorders. Long-term treatment with glucocorticoids results in osteoporosis and increased risk of fractures. Much remains unclear regarding the effects ... ...

Abstract	Vitamin D is essential for bone function and deficiency in active vitamin D hormone can lead to bone disorders. Long-term treatment with glucocorticoids results in osteoporosis and increased risk of fractures. Much remains unclear regarding the effects of these compounds in bone cells. In the current study, human osteosarcoma Saos-2 cells and primary human osteoblasts were found to express mRNA for the vitamin D receptor as well as activating and deactivating enzymes in vitamin D3 metabolism. These bone cells exhibited CYP24A1-mediated 24-hydroxylation which is essential for deactivation of the active vitamin form. However, bioactivating vitamin D3 hydroxylase activities could not be detected in either of these cells. Several glucocorticoids, including prednisolone, down regulated CYP24A1 mRNA and CYP24A1-mediated 24-hydroxylase activity in both Saos-2 and primary human osteoblasts. Also, prednisolone significantly suppressed a human CYP24A1 promoter-luciferase reporter gene in Saos-2 cells co-transfected with the glucocorticoid receptor. Thus, the results of the present study show suppression by glucocorticoids on CYP24A1 mRNA, CYP24A1-mediated metabolism and CYP24A1 promoter activity in human osteoblast-like cells. As part of this study we examined if glucocorticoids are formed locally in Saos-2 cells. The experiments indicate formation of 11-deoxycortisol, a steroid with glucocorticoid activity, which can bind the glucocorticoid receptor. Our data showing suppression by glucocorticoids on CYP24A1 expression in human osteoblasts suggest a previously unknown mechanism for effects of glucocorticoids in human bone, where these compounds may interfere with regulation of active vitamin D levels.
Keywords	calcitriol receptors ; cholecalciferol ; enzyme activity ; enzymes ; gene expression regulation ; glucocorticoid receptors ; glucocorticoids ; humans ; messenger RNA ; metabolism ; osteoblasts ; osteoporosis ; osteosarcoma ; prednisolone ; reporter genes ; risk
Language	English
Dates of publication	2019-1001
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2019.110525
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 1127: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article: Acute effects of methcathinone and manganese in mice: A dose response study.

Asser, Andres / Kõks, Sulev / Soomets, Ursel / Terasmaa, Anton / Sauk, Martin / Eltermaa, Mall / Piip, Piret / Ubhayasekera, Kumari / Bergquist, Jonas / Taba, Pille

Heliyon

2019 Volume 5, Issue 9, Page(s) e02475

Abstract: An intravenously injectable illicit drug made by mixing pseudoephedrine, potassium permanganate, vinegar and water, yielding methcathinone (Mcat) and manganese (Mn), induces an extrapyramidal syndrome with parkinsonism, dystonia, gait and balance ... ...

Abstract	An intravenously injectable illicit drug made by mixing pseudoephedrine, potassium permanganate, vinegar and water, yielding methcathinone (Mcat) and manganese (Mn), induces an extrapyramidal syndrome with parkinsonism, dystonia, gait and balance disorders similar to manganism. Although the cause of the syndrome is largely attributed to Mn, the interaction of the drug's individual components is not known and the role of Mcat is possibly underestimated. Aim of the present study was to analyze dose-dependent behavioral effects of the mixture and its two main active components Mcat and Mn in an acute setting and determine the lethal doses of each substance. Three groups of C57BL/6 mice were injected intraperitoneally with (1) the drug mixture containing 10, 25, 50, 100 or 150 mg of Mcat and respectively 1.6, 3.8, 6.9, 17.1 and 22.6 mg of Mn per kilogram of body weight; (2) 10, 25, 50, 100, 150, 200 or 300 mg of racemic Mcat/kg of body weight; (3) MnCl
Language	English
Publishing date	2019-09-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2019.e02475
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Article ; Online: Effects of glucocorticoids on vitamin D

Zayny, Ahmad / Almokhtar, Mokhtar / Wikvall, Kjell / Ljunggren, Östen / Ubhayasekera, Kumari / Bergquist, Jonas / Kibar, Pinar / Norlin, Maria

Molecular and cellular endocrinology

2019 Volume 496, Page(s) 110525

Abstract	Vitamin D is essential for bone function and deficiency in active vitamin D hormone can lead to bone disorders. Long-term treatment with glucocorticoids results in osteoporosis and increased risk of fractures. Much remains unclear regarding the effects of these compounds in bone cells. In the current study, human osteosarcoma Saos-2 cells and primary human osteoblasts were found to express mRNA for the vitamin D receptor as well as activating and deactivating enzymes in vitamin D
MeSH term(s)	Cell Line, Tumor ; Cholecalciferol/metabolism ; Gene Expression Regulation, Enzymologic/drug effects ; Glucocorticoids/pharmacology ; Humans ; Osteoblasts/cytology ; Osteoblasts/enzymology ; Promoter Regions, Genetic ; Vitamin D3 24-Hydroxylase/biosynthesis ; Vitamin D3 24-Hydroxylase/genetics
Chemical Substances	Glucocorticoids ; Cholecalciferol (1C6V77QF41) ; CYP24A1 protein, human (EC 1.14.15.16) ; Vitamin D3 24-Hydroxylase (EC 1.14.15.16)
Language	English
Publishing date	2019-07-25
Publishing country	Ireland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	187438-x
ISSN	1872-8057 ; 0303-7207
ISSN (online)	1872-8057
ISSN	0303-7207
DOI	10.1016/j.mce.2019.110525
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Zs.A 1127: Show issues

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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Phase 1 study to access safety, tolerability, pharmacokinetics, and pharmacodynamics of kynurenine in healthy volunteers.

Al-Karagholi, Mohammad Al-Mahdi / Hansen, Jakob Møller / Abou-Kassem, Dalia / Hansted, Anna Koldbro / Ubhayasekera, Kumari / Bergquist, Jonas / Vécsei, László / Jansen-Olesen, Inger / Ashina, Messoud

Pharmacology research & perspectives

2021 Volume 9, Issue 2, Page(s) e00741

Abstract: The kynurenine pathway (KP) is the main path for tryptophan metabolism, and it represents a multitude of potential sites for drug discovery in neuroscience, including pain, stroke, and epilepsy. L-kynurenine (LKYN), the first active metabolite in the ... ...

Abstract	The kynurenine pathway (KP) is the main path for tryptophan metabolism, and it represents a multitude of potential sites for drug discovery in neuroscience, including pain, stroke, and epilepsy. L-kynurenine (LKYN), the first active metabolite in the pathway, emerges to be a prodrug targeting glutamate receptors. The safety, tolerability, pharmacokinetics, and pharmacodynamics of LKYN in humans have not been previously investigated. In an open-label, single ascending dose study, six participants received an intravenous infusion of 50, 100, and 150 µg/kg LKYN and new six participants received an intravenous infusion of 0.3, 0.5, 1, and 5 mg/kg LKYN. To compare the pharmacological effects between species, we investigated in vivo the vascular effects of LKYN in rats. In humans, LKYN was safe and well-tolerated at all dose levels examined. After infusion, LKYN plasma concentration increased significantly over time 3.23 ± 1.12 µg/mL (after 50 µg/kg), 4.04 ± 1.1 µg/mL (after 100 µg/kg), and 5.25 ± 1.01 µg/mL (after 150 µg/kg) (p ≤ 0.001). We observed no vascular changes after infusion compared with baseline. In rats, LKYN had no effect on HR and MAP and caused no dilation of dural and pial arteries. This first-in-human study of LKYN showed that LKYN was safe and well-tolerated after intravenous infusion up to 5 mg/kg over 20 minutes. The lack of change in LKYN metabolites in plasma suggests a relatively slow metabolism of LKYN and no or little feed-back effect of LKYN on its synthesis. The therapeutic potential of LKYN in stroke and epilepsy should be explored in future studies in humans.
MeSH term(s)	Adult ; Animals ; Blood Flow Velocity/drug effects ; Cerebrovascular Circulation/drug effects ; Cross-Over Studies ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical ; Epilepsy/drug therapy ; Female ; Healthy Volunteers ; Humans ; Infusions, Intravenous ; Kynurenine/administration & dosage ; Kynurenine/adverse effects ; Kynurenine/pharmacokinetics ; Male ; Pilot Projects ; Prodrugs/administration & dosage ; Prodrugs/adverse effects ; Prodrugs/pharmacokinetics ; Rats ; Stroke/drug therapy ; Young Adult
Chemical Substances	Prodrugs ; Kynurenine (343-65-7)
Language	English
Publishing date	2021-03-07
Publishing country	United States
Document type	Clinical Trial, Phase I ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2740389-0
ISSN	2052-1707 ; 2052-1707
ISSN (online)	2052-1707
ISSN	2052-1707
DOI	10.1002/prp2.741
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Acute effects of methcathinone and manganese in mice: A dose response study

Asser, Andres / Kõks, Sulev / Soomets, Ursel / Terasmaa, Anton / Sauk, Martin / Eltermaa, Mall / Piip, Piret / Ubhayasekera, Kumari / Bergquist, Jonas / Taba, Pille

Heliyon. 2019 Sept., v. 5, no. 9 p.e02475-

2019

Abstract	An intravenously injectable illicit drug made by mixing pseudoephedrine, potassium permanganate, vinegar and water, yielding methcathinone (Mcat) and manganese (Mn), induces an extrapyramidal syndrome with parkinsonism, dystonia, gait and balance disorders similar to manganism. Although the cause of the syndrome is largely attributed to Mn, the interaction of the drug's individual components is not known and the role of Mcat is possibly underestimated. Aim of the present study was to analyze dose-dependent behavioral effects of the mixture and its two main active components Mcat and Mn in an acute setting and determine the lethal doses of each substance. Three groups of C57BL/6 mice were injected intraperitoneally with (1) the drug mixture containing 10, 25, 50, 100 or 150 mg of Mcat and respectively 1.6, 3.8, 6.9, 17.1 and 22.6 mg of Mn per kilogram of body weight; (2) 10, 25, 50, 100, 150, 200 or 300 mg of racemic Mcat/kg of body weight; (3) MnCl₂ 10, 25 or 50 mg/kg of body weight. Locomotor activity of the animals, various signs and time of death were recorded. Lower doses (10 and 25 mg/kg) of Mcat had a clear motor activity stimulating effect and this was clearly dose-dependent. High doses of Mcat produced epileptic seizures in 74% of the animals and became lethal with the highest doses. Similarly, the mixture had a clear dose-dependent stimulating effect and the higher doses became lethal. The LD₅₀ of the pseudoephedrine mixture was 110.2 mg of Mcat/kg and for pure Mcat 201.7 mg/kg. Mn did not prove to be lethal in doses up to 50 mg/kg, but had a strong dose dependent inhibitory effect on the animals' behavior. Our data reveal that both Mn and Mcat have a significant role in the toxicity of the mixture.
Keywords	Parkinson disease ; body weight ; death ; dose response ; gait ; illicit drugs ; intravenous injection ; locomotion ; manganese ; potassium permanganate ; pseudoephedrine ; toxicity ; vinegars ; Neuroscience ; Toxicology ; Behavioral neuroscience ; Dose-response relationship ; Nervous system ; Neurotoxicology ; Ephedrone ; Toxic parkinsonism ; Methcathinone
Language	English
Dates of publication	2019-09
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	NAL-AP-2-clean ; Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2019.e02475
Database	NAL-Catalogue (AGRICOLA)

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