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  1. Article ; Online: Assay of NAT Activity.

    Uyama, Toru / Ueda, Natsuo

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2576, Page(s) 213–224

    Abstract: In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive N-acylethanolamines, in which an acyl chain is transferred from the sn-1 position of the donor phospholipid, such as phosphatidylcholine, to ... ...

    Abstract In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive N-acylethanolamines, in which an acyl chain is transferred from the sn-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of N-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca
    MeSH term(s) Acyltransferases/metabolism ; Animals ; Phosphatidylcholines ; Phosphatidylethanolamines/metabolism ; Phospholipases A/metabolism ; Phospholipids ; Rats
    Chemical Substances Phosphatidylcholines ; Phosphatidylethanolamines ; Phospholipids ; Acyltransferases (EC 2.3.-) ; Phospholipases A (EC 3.1.1.32)
    Language English
    Publishing date 2022-09-23
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2728-0_17
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  2. Article ; Online: Assay of NAAA Activity.

    Tsuboi, Kazuhito / Ueda, Natsuo

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2576, Page(s) 261–274

    Abstract: N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, and its ... ...

    Abstract N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, and its specific inhibitors are shown to exert anti-inflammatory and analgesic actions in animal models. Therefore, these inhibitors are expected as a new class of therapeutic agents. Here, we introduce an NAAA assay system, using [
    MeSH term(s) Amides ; Amidohydrolases/chemistry ; Analgesics/pharmacology ; Animals ; Anti-Inflammatory Agents/pharmacology ; Enzyme Inhibitors/pharmacology ; Ethanolamines ; Palmitic Acids
    Chemical Substances Amides ; Analgesics ; Anti-Inflammatory Agents ; Enzyme Inhibitors ; Ethanolamines ; N-acylethanolamines ; Palmitic Acids ; palmidrol (6R8T1UDM3V) ; Amidohydrolases (EC 3.5.-)
    Language English
    Publishing date 2022-09-24
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2728-0_22
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  3. Article ; Online: PLAAT1 expression triggers fragmentation of mitochondria in an enzyme activity-dependent manner.

    Sikder, Mohammad Mamun / Uyama, Toru / Sasaki, Sumire / Kawai, Katsuhisa / Araki, Nobukazu / Ueda, Natsuo

    Journal of biochemistry

    2024  Volume 175, Issue 1, Page(s) 101–113

    Abstract: The phospholipase A and acyltransferase (PLAAT) family is a protein family consisting of five members (PLAAT1-5), which acts as phospholipid-metabolizing enzymes with phospholipase A1/A2 and N-acyltransferase activities. Since we previously reported that ...

    Abstract The phospholipase A and acyltransferase (PLAAT) family is a protein family consisting of five members (PLAAT1-5), which acts as phospholipid-metabolizing enzymes with phospholipase A1/A2 and N-acyltransferase activities. Since we previously reported that the overexpression of PLAAT3 in mammalian cells causes the specific disappearance of peroxisomes, in the present study we examined a possible effect of PLAAT1 on organelles. We prepared HEK293 cells expressing mouse PLAAT1 in a doxycycline-dependent manner and found that the overexpression of PLAAT1 resulted in the transformation of mitochondria from the original long rod shape to a round shape, as well as their fragmentation. In contrast, the overexpression of a catalytically inactive point mutant of PLAAT1 did not generate any morphological change in mitochondria, suggesting the involvement of catalytic activity. PLAAT1 expression also caused the reduction of peroxisomes, while the levels of the marker proteins for ER, Golgi apparatus and lysosomes were almost unchanged. In PLAAT1-expressing cells, the level of dynamin-related protein 1 responsible for mitochondrial fission was increased, whereas those of optic atrophy 1 and mitofusin 2, both of which are responsible for mitochondrial fusion, were reduced. These results suggest a novel role of PLAAT1 in the regulation of mitochondrial biogenesis.
    MeSH term(s) Humans ; Animals ; Mice ; HEK293 Cells ; Mitochondria/metabolism ; Peroxisomes/metabolism ; Golgi Apparatus/metabolism ; Acyltransferases/metabolism ; Mammals
    Chemical Substances Acyltransferases (EC 2.3.-)
    Language English
    Publishing date 2024-01-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 218073-x
    ISSN 1756-2651 ; 0021-924X
    ISSN (online) 1756-2651
    ISSN 0021-924X
    DOI 10.1093/jb/mvad079
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  4. Article ; Online: Roles of Endocannabinoids and Endocannabinoid-Like Molecules in Energy Homeostasis and Metabolic Regulation: A Nutritional Perspective.

    Rahman, S M Khaledur / Uyama, Toru / Hussain, Zahir / Ueda, Natsuo

    Annual review of nutrition

    2021  Volume 41, Page(s) 177–202

    Abstract: The endocannabinoid system is involved in signal transduction in mammals. It comprises principally G protein-coupled cannabinoid receptors and their endogenous agonists, called endocannabinoids, as well as the enzymes and transporters responsible for the ...

    Abstract The endocannabinoid system is involved in signal transduction in mammals. It comprises principally G protein-coupled cannabinoid receptors and their endogenous agonists, called endocannabinoids, as well as the enzymes and transporters responsible for the metabolism of endocannabinoids. Two arachidonic acid-containing lipid molecules, arachidonoylethanolamide (anandamide) and 2-arachidonoylglycerol, function as endocannabinoids.
    MeSH term(s) Animals ; Endocannabinoids/metabolism ; Homeostasis ; Humans ; Mammals/metabolism ; Obesity
    Chemical Substances Endocannabinoids
    Language English
    Publishing date 2021-06-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 406980-8
    ISSN 1545-4312 ; 0199-9885
    ISSN (online) 1545-4312
    ISSN 0199-9885
    DOI 10.1146/annurev-nutr-043020-090216
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: GDE7 produces cyclic phosphatidic acid in the ER lumen functioning as a lysophospholipid mediator.

    Kitakaze, Keisuke / Ali, Hanif / Kimoto, Raiki / Takenouchi, Yasuhiro / Ishimaru, Hironobu / Yamashita, Atsushi / Ueda, Natsuo / Tanaka, Tamotsu / Okamoto, Yasuo / Tsuboi, Kazuhito

    Communications biology

    2023  Volume 6, Issue 1, Page(s) 524

    Abstract: Cyclic phosphatidic acid (cPA) is a lipid mediator, which regulates adipogenic differentiation and glucose homeostasis by suppressing nuclear peroxisome proliferator-activated receptor γ (PPARγ). Glycerophosphodiesterase 7 (GDE7) is a ... ...

    Abstract Cyclic phosphatidic acid (cPA) is a lipid mediator, which regulates adipogenic differentiation and glucose homeostasis by suppressing nuclear peroxisome proliferator-activated receptor γ (PPARγ). Glycerophosphodiesterase 7 (GDE7) is a Ca
    MeSH term(s) Mice ; Animals ; Humans ; Phosphatidic Acids/metabolism ; PPAR gamma/genetics ; PPAR gamma/metabolism ; Lysophospholipids/metabolism ; Endoplasmic Reticulum/metabolism ; Phosphoric Diester Hydrolases/genetics
    Chemical Substances Phosphatidic Acids ; glycerophosphodiester phosphodiesterase (EC 3.1.4.46) ; PPAR gamma ; Lysophospholipids ; GDE7 protein, mouse (EC 3.1.4.-) ; Phosphoric Diester Hydrolases (EC 3.1.4.-)
    Language English
    Publishing date 2023-05-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2399-3642
    ISSN (online) 2399-3642
    DOI 10.1038/s42003-023-04900-4
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  6. Article ; Online: Vasoactive intestinal peptide blockade suppresses tumor growth by regulating macrophage polarization and function in CT26 tumor-bearing mice.

    Kittikulsuth, Wararat / Nakano, Daisuke / Kitada, Kento / Uyama, Toru / Ueda, Natsuo / Asano, Eisuke / Okano, Keiichi / Matsuda, Yoko / Nishiyama, Akira

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 927

    Abstract: Macrophages are a major population of immune cells in solid cancers, especially colorectal cancers. Tumor-associated macrophages (TAMs) are commonly divided into M1-like (tumor suppression) and M2-like (tumor promotion) phenotypes. Vasoactive intestinal ... ...

    Abstract Macrophages are a major population of immune cells in solid cancers, especially colorectal cancers. Tumor-associated macrophages (TAMs) are commonly divided into M1-like (tumor suppression) and M2-like (tumor promotion) phenotypes. Vasoactive intestinal peptide (VIP) is an immunoregulatory neuropeptide with a potent anti-inflammatory function. Inhibition of VIP signaling has been shown to increase CD8
    MeSH term(s) Animals ; Mice ; Colonic Neoplasms/pathology ; Macrophages/metabolism ; Mice, SCID ; Receptors, Vasoactive Intestinal Peptide/metabolism ; Receptors, Vasoactive Intestinal Peptide, Type II/metabolism ; Signal Transduction ; Vasoactive Intestinal Peptide/antagonists & inhibitors ; Vasoactive Intestinal Peptide/metabolism ; RAW 264.7 Cells
    Chemical Substances Receptors, Vasoactive Intestinal Peptide ; Receptors, Vasoactive Intestinal Peptide, Type II ; Vasoactive Intestinal Peptide (37221-79-7) ; Vipr2 protein, mouse
    Language English
    Publishing date 2023-01-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-28073-6
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  7. Article ; Online: β-1,4-Galactan suppresses lipid synthesis in sebaceous gland cells via TLR4.

    Ayaki, Satomi / Mii, Tomohiro / Matsuno, Kosuke / Tokuyama, Takaaki / Tokuyama, Takahito / Tokuyama, Takashi / Uyama, Toru / Ueda, Natsuo

    Journal of biochemistry

    2023  Volume 173, Issue 2, Page(s) 85–94

    Abstract: Sebum is a lipid mixture secreted from sebaceous glands of the skin. The excessive secretion of sebum causes acne vulgaris and seborrheic dermatitis, while its deficiency causes xerosis. Therefore, the appropriate control of sebum secretion is crucially ... ...

    Abstract Sebum is a lipid mixture secreted from sebaceous glands of the skin. The excessive secretion of sebum causes acne vulgaris and seborrheic dermatitis, while its deficiency causes xerosis. Therefore, the appropriate control of sebum secretion is crucially important to keep the skin healthy. In the present study, we evaluated the effects of naturally occurring polysaccharides on lipid biosynthesis in hamster sebaceous gland cells. Among the tested polysaccharides, β-1,4-galactan, the main chain of type I arabinogalactan, most potently suppressed lipid synthesis in the sebaceous gland cells as analysed by oil red O staining. Toll-like receptor (TLR)4 inhibitors counteracted this suppressive effect and lipopolysaccharide, a TLR4 ligand, mimicked this effect, suggesting the involvement of the TLR4 signalling pathway. In the cells β-1,4-galactan significantly decreased mRNA levels of lipogenesis-related transcription factors (peroxisomeGraphical Abstract$\includegraphics{\bwartpath }$ proliferator-activated receptor γ and sterol regulatory element-binding protein 1) and enzymes (acetyl-CoA carboxylase and fatty acid synthase) as well as the glucose transporter GLUT4. Furthermore, β-1,4-galactan increased the production of lactic acid serving as a natural moisturizing factor and enhanced the proliferation of sebaceous gland cells. These results suggest potential of β-1,4-galactan as a material with therapeutic and cosmetic values for the skin.
    MeSH term(s) Animals ; Cricetinae ; Sebaceous Glands/metabolism ; Lipogenesis ; Toll-Like Receptor 4/metabolism ; Lipids ; Galactans/metabolism ; Galactans/pharmacology
    Chemical Substances Toll-Like Receptor 4 ; Lipids ; Galactans
    Language English
    Publishing date 2023-01-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 218073-x
    ISSN 1756-2651 ; 0021-924X
    ISSN (online) 1756-2651
    ISSN 0021-924X
    DOI 10.1093/jb/mvac085
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  8. Article ; Online: Assay of NAT Activity.

    Uyama, Toru / Ueda, Natsuo

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1412, Page(s) 113–122

    Abstract: In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive N-acylethanolamines, in which an acyl chain is transferred from the sn-1 position of the donor phospholipid, such as phosphatidylcholine, to ... ...

    Abstract In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive N-acylethanolamines, in which an acyl chain is transferred from the sn-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of N-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca(2+), and hence it has been referred to as Ca(2+)-dependent NAT. On the other hand, members of the phospholipase A/acyltransferase (PLA/AT) family (also known as HRAS-like suppressor family) show Ca(2+)-independent NAT activity. In this chapter, we describe (1) partial purification of Ca(2+)-dependent NAT from rat brain, (2) purification of recombinant PLA/AT-2, and (3) NAT assay using radiolabeled substrate.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-3539-0_12
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  9. Article ; Online: Assay of NAAA Activity.

    Tsuboi, Kazuhito / Ueda, Natsuo

    Methods in molecular biology (Clifton, N.J.)

    2016  Volume 1412, Page(s) 137–148

    Abstract: N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. Since NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, its ... ...

    Abstract N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. Since NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, its specific inhibitors are expected as a new class of anti-inflammatory and analgesic agents. Here, we introduce an NAAA assay system, using [(14)C]palmitoylethanolamide and thin-layer chromatography. The preparation of NAAA enzyme from native and recombinant sources as well as the chemical synthesis of N-[1'-(14)C]palmitoyl-ethanolamine is also described.
    Language English
    Publishing date 2016
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-3539-0_15
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  10. Article: Endocannabinoids and related

    Tsuboi, Kazuhito / Uyama, Toru / Okamoto, Yasuo / Ueda, Natsuo

    Inflammation and regeneration

    2018  Volume 38, Page(s) 28

    Abstract: ... The ... ...

    Abstract The plant
    Language English
    Publishing date 2018-10-01
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2051471-2
    ISSN 1880-9693 ; 0389-4290
    ISSN 1880-9693 ; 0389-4290
    DOI 10.1186/s41232-018-0086-5
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