LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 70

Search options

  1. Article: A multiscale modelling approach for

    Usai, Alessandro / Pittman, Jon K / Theodoropoulos, Constantinos

    Biotechnology reports (Amsterdam, Netherlands)

    2022  Volume 36, Page(s) e00771

    Abstract: Haematococcus ... ...

    Abstract Haematococcus pluvialis
    Language English
    Publishing date 2022-10-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2022.e00771
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Structured population balances to support microalgae-based processes: Review of the state-of-art and perspectives analysis.

    Usai, Alessandro / Theodoropoulos, Constantinos / Di Caprio, Fabrizio / Altimari, Pietro / Cao, Giacomo / Concas, Alessandro

    Computational and structural biotechnology journal

    2023  Volume 21, Page(s) 1169–1188

    Abstract: Design and optimization of microalgae processes have traditionally relied on the application of unsegregated mathematical models, thus neglecting the impact of cell-to-cell heterogeneity. However, there is experimental evidence that the latter one, ... ...

    Abstract Design and optimization of microalgae processes have traditionally relied on the application of unsegregated mathematical models, thus neglecting the impact of cell-to-cell heterogeneity. However, there is experimental evidence that the latter one, including but not limited to variation in mass/size, internal composition and cell cycle phase, can play a crucial role in both cultivation and downstream processes. Population balance equations (PBEs) represent a powerful approach to develop mathematical models describing the effect of cell-to-cell heterogeneity. In this work, the potential of PBEs for the analysis and design of microalgae processes are discussed. A detailed review of PBE applications to microalgae cultivation, harvesting and disruption is reported. The review is largely focused on the application of the univariate size/mass structured PBE, where the size/mass is the only internal variable used to identify the cell state. Nonetheless, the need, addressed by few studies, for additional or alternative internal variables to identify the cell cycle phase and/or provide information about the internal composition is discussed. Through the review, the limitations of previous studies are described, and areas are identified where the development of more reliable PBE models, driven by the increasing availability of single-cell experimental data, could support the understanding and purposeful exploitation of the mechanisms determining cell-to-cell heterogeneity.
    Language English
    Publishing date 2023-01-31
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2694435-2
    ISSN 2001-0370
    ISSN 2001-0370
    DOI 10.1016/j.csbj.2023.01.042
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Establishment of Zebrafish Patient-derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing.

    Usai, Alice / Di Franco, Gregorio / Gabellini, Chiara / Morelli, Luca / Raffa, Vittoria

    Journal of visualized experiments : JoVE

    2023  , Issue 195

    Abstract: Cancer is one of the main causes of death worldwide, and the incidence of many types of cancer continues to increase. Much progress has been made in terms of screening, prevention, and treatment; however, preclinical models that predict the ... ...

    Abstract Cancer is one of the main causes of death worldwide, and the incidence of many types of cancer continues to increase. Much progress has been made in terms of screening, prevention, and treatment; however, preclinical models that predict the chemosensitivity profile of cancer patients are still lacking. To fill this gap, an in vivo patient-derived xenograft model was developed and validated. The model was based on zebrafish (Danio rerio) embryos at 2 days post fertilization, which were used as recipients of xenograft fragments of tumor tissue taken from a patient's surgical specimen. It is also worth noting that bioptic samples were not digested or disaggregated, in order to maintain the tumor microenvironment, which is crucial in terms of analyzing tumor behavior and the response to therapy. The protocol details a method for establishing zebrafish-based patient-derived xenografts (zPDXs) from primary solid tumor surgical resection. After screening by an anatomopathologist, the specimen is dissected using a scalpel blade. Necrotic tissue, vessels, or fatty tissue are removed and then chopped into 0.3 mm x 0.3 mm x 0.3 mm pieces. The pieces are then fluorescently labeled and xenotransplanted into the perivitelline space of zebrafish embryos. A large number of embryos can be processed at a low cost, enabling high-throughput in vivo analyses of the chemosensitivity of zPDXs to multiple anticancer drugs. Confocal images are routinely acquired to detect and quantify the apoptotic levels induced by chemotherapy treatment compared to the control group. The xenograft procedure has a significant time advantage, since it can be completed in a single day, providing a reasonable time window to carry out a therapeutic screening for co-clinical trials.
    MeSH term(s) Humans ; Animals ; Zebrafish ; Heterografts ; Pancreatic Neoplasms/drug therapy ; Adipose Tissue ; Disease Models, Animal ; Tumor Microenvironment ; Pancreatic Neoplasms
    Language English
    Publishing date 2023-05-12
    Publishing country United States
    Document type Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63744
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Zebrafish Avatar to Develop Precision Breast Cancer Therapies.

    Corsinovi, Debora / Usai, Alice / Sarlo, Miriam De / Giannaccini, Martina / Ori, Michela

    Anti-cancer agents in medicinal chemistry

    2021  Volume 22, Issue 4, Page(s) 748–759

    Abstract: Background: Zebrafish (Danio rerio) is a vertebrate that has become a popular alternative model for the cellular and molecular study of human tumors and for drug testing and validating approaches. Notably, zebrafish embryos, thanks to their ... ...

    Abstract Background: Zebrafish (Danio rerio) is a vertebrate that has become a popular alternative model for the cellular and molecular study of human tumors and for drug testing and validating approaches. Notably, zebrafish embryos, thanks to their accessibility, allow rapid collection of in vivo results prodromal to validation in the murine models in respect to the 3R principles. The generation of tumor xenograft in zebrafish embryos and larvae, or zebrafish avatar, represents a unique opportunity to study tumor growth, angiogenesis, cell invasion and metastatic dissemination, interaction between tumor and host in vivo avoiding immunogenic rejection, representing a promising platform for the translational research and personalized therapies.
    Objective: In this mini-review, we report recent advances in breast cancer research and drug testing that took advantage of the zebrafish xenograft model using both breast cancer cell lines and patient's biopsy.
    Conclusion: Patient derived xenograft, together with the gene editing, the omics biotechnology, the in vivo time lapse imaging and the high-throughput screening that are already set up and largely used in zebrafish, could represent a step forward towards precision and personalized medicine in the breast cancer research field.
    MeSH term(s) Animals ; Breast Neoplasms/drug therapy ; Disease Models, Animal ; Female ; Heterografts ; Humans ; Mice ; Precision Medicine/methods ; Xenograft Model Antitumor Assays ; Zebrafish
    Language English
    Publishing date 2021-04-01
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2217610-X
    ISSN 1875-5992 ; 1871-5206
    ISSN (online) 1875-5992
    ISSN 1871-5206
    DOI 10.2174/1871520621666210402111634
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5583: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Time-correlated single photon Raman spectroscopy at megahertz repetition rates.

    Finlayson, Neil / Usai, Andrea / Brown, Gillian E / McEwan, Heather / Erdogan, Ahmet T / Campbell, Colin J / Henderson, Robert K

    Optics letters

    2021  Volume 46, Issue 17, Page(s) 4104–4107

    Abstract: Significant improvements in time-correlated single photon counting (TCSPC) Raman spectroscopy acquisition times can be achieved through exploitation of megahertz (MHz) laser repetition rates. We have developed a TCSPC Raman spectroscopy system based on a ...

    Abstract Significant improvements in time-correlated single photon counting (TCSPC) Raman spectroscopy acquisition times can be achieved through exploitation of megahertz (MHz) laser repetition rates. We have developed a TCSPC Raman spectroscopy system based on a high peak power (>40
    Language English
    Publishing date 2021-09-06
    Publishing country United States
    Document type Journal Article
    ISSN 1539-4794
    ISSN (online) 1539-4794
    DOI 10.1364/OL.434418
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Zebrafish Patient-Derived Xenograft Model to Predict Treatment Outcomes of Colorectal Cancer Patients.

    Di Franco, Gregorio / Usai, Alice / Piccardi, Margherita / Cateni, Perla / Palmeri, Matteo / Pollina, Luca Emanuele / Gaeta, Raffaele / Marmorino, Federica / Cremolini, Chiara / Dente, Luciana / Massolo, Alessandro / Raffa, Vittoria / Morelli, Luca

    Biomedicines

    2022  Volume 10, Issue 7

    Abstract: The use of zebrafish embryos for personalized medicine has become increasingly popular. We present a co-clinical trial aiming to evaluate the use of zPDX (zebrafish Patient-Derived Xenografts) in predicting the response to chemotherapy regimens used for ... ...

    Abstract The use of zebrafish embryos for personalized medicine has become increasingly popular. We present a co-clinical trial aiming to evaluate the use of zPDX (zebrafish Patient-Derived Xenografts) in predicting the response to chemotherapy regimens used for colorectal cancer patients. zPDXs are generated by xenografting tumor tissues in two days post-fertilization zebrafish embryos. zPDXs were exposed to chemotherapy regimens (5-FU, FOLFIRI, FOLFOX, FOLFOXIRI) for 48 h. We used a linear mixed effect model to evaluate the zPDX-specific response to treatments showing for 4/36 zPDXs (11%), a statistically significant reduction of tumor size compared to controls. We used the RECIST criteria to compare the outcome of each patient after chemotherapy with the objective response of its own zPDX model. Of the 36 patients enrolled, 8 metastatic colorectal cancer (mCRC), response rate after first-line therapy, and the zPDX chemosensitivity profile were available. Of eight mCRC patients, five achieved a partial response and three had a stable disease. In 6/8 (75%) we registered a concordance between the response of the patient and the outcomes reported in the corresponding zPDX. Our results provide evidence that the zPDX model can reflect the outcome in mCRC patients, opening a new frontier to personalized medicine.
    Language English
    Publishing date 2022-06-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines10071474
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article ; Online: Nonverbal stimuli in a virtual aisle

    Porcheddu, D. / Usai, A. / Venturi, A.

    Does the shelf you choose matter?

    2011  

    Abstract: S.215-231 ... In this work, we evaluate the phenomenon of asymmetric perception of some nonverbal stimuli located in an aisle of a virtual store. In a stimulus detection experiment, with a number of potentially confounding variables held under control, it ... ...

    Abstract S.215-231

    In this work, we evaluate the phenomenon of asymmetric perception of some nonverbal stimuli located in an aisle of a virtual store. In a stimulus detection experiment, with a number of potentially confounding variables held under control, it was found that the nonverbal stimuli utilized (belonging to the color, size, and shape families) were perceived more rapidly when placed on shelves on the left rather than on the right of the observer. This response pattern was constant regardless the nature of stimulus and the gender of observer. The experiment also showed the following sequence, in descending order of perception rapidity: color-shape-size. Our results appear consistent with the predictions of the brain laterality theory. Overall, our article provides an initial contribution to extend the results of Rettie and Brewer (Journal of Product and Brand Management 2000; 9, no. 1: 56-70) pioneering work obtained by reference to specific problems of package design to the po sitioning of items with respect to instore traffic flow. Limitations and managerial implications of our work are discussed.

    21

    Nr.3
    Subject code 150
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article: Zebrafish Patient-Derived Xenografts Identify Chemo-Response in Pancreatic Ductal Adenocarcinoma Patients.

    Usai, Alice / Di Franco, Gregorio / Piccardi, Margherita / Cateni, Perla / Pollina, Luca Emanuele / Vivaldi, Caterina / Vasile, Enrico / Funel, Niccola / Palmeri, Matteo / Dente, Luciana / Falcone, Alfredo / Giunchi, Dimitri / Massolo, Alessandro / Raffa, Vittoria / Morelli, Luca

    Cancers

    2021  Volume 13, Issue 16

    Abstract: It is increasingly evident the necessity of new predictive tools for the treatment of pancreatic ductal adenocarcinoma in a personalized manner. We present a co-clinical trial testing the predictiveness of zPDX (zebrafish patient-derived xenograft) for ... ...

    Abstract It is increasingly evident the necessity of new predictive tools for the treatment of pancreatic ductal adenocarcinoma in a personalized manner. We present a co-clinical trial testing the predictiveness of zPDX (zebrafish patient-derived xenograft) for assessing if patients could benefit from a therapeutic strategy (ClinicalTrials.gov: XenoZ, NCT03668418). zPDX are generated xenografting tumor tissues in zebrafish embryos. zPDX were exposed to chemotherapy regimens commonly used. We considered a zPDX a responder (R) when a decrease ≥50% in the relative tumor area was reported; otherwise, we considered them a non-responder (NR). Patients were classified as Responder if their own zPDX was classified as an R for the chemotherapy scheme she/he received an adjuvant treatment; otherwise, we considered them a Non-Responder. We compared the cancer recurrence rate at 1 year after surgery and the disease-free survival (DFS) of patients of both groups. We reported a statistically significant higher recurrence rate in the Non-Responder group: 66.7% vs. 14.3% (
    Language English
    Publishing date 2021-08-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13164131
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: Precision Medicine in Head and Neck Cancers: Genomic and Preclinical Approaches.

    Miserocchi, Giacomo / Spadazzi, Chiara / Calpona, Sebastiano / De Rosa, Francesco / Usai, Alice / De Vita, Alessandro / Liverani, Chiara / Cocchi, Claudia / Vanni, Silvia / Calabrese, Chiara / Bassi, Massimo / De Luca, Giovanni / Meccariello, Giuseppe / Ibrahim, Toni / Schiavone, Marco / Mercatali, Laura

    Journal of personalized medicine

    2022  Volume 12, Issue 6

    Abstract: Head and neck cancers (HNCs) represent the sixth most widespread malignancy worldwide. Surgery, radiotherapy, chemotherapeutic and immunotherapeutic drugs represent the main clinical approaches for HNC patients. Moreover, HNCs are characterised by an ... ...

    Abstract Head and neck cancers (HNCs) represent the sixth most widespread malignancy worldwide. Surgery, radiotherapy, chemotherapeutic and immunotherapeutic drugs represent the main clinical approaches for HNC patients. Moreover, HNCs are characterised by an elevated mutational load; however, specific genetic mutations or biomarkers have not yet been found. In this scenario, personalised medicine is showing its efficacy. To study the reliability and the effects of personalised treatments, preclinical research can take advantage of next-generation sequencing and innovative technologies that have been developed to obtain genomic and multi-omic profiles to drive personalised treatments. The crosstalk between malignant and healthy components, as well as interactions with extracellular matrices, are important features which are responsible for treatment failure. Preclinical research has constantly implemented in vitro and in vivo models to mimic the natural tumour microenvironment. Among them, 3D systems have been developed to reproduce the tumour mass architecture, such as biomimetic scaffolds and organoids. In addition, in vivo models have been changed over the last decades to overcome problems such as animal management complexity and time-consuming experiments. In this review, we will explore the new approaches aimed to improve preclinical tools to study and apply precision medicine as a therapeutic option for patients affected by HNCs.
    Language English
    Publishing date 2022-05-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662248-8
    ISSN 2075-4426
    ISSN 2075-4426
    DOI 10.3390/jpm12060854
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article: A Model of a Zebrafish Avatar for Co-Clinical Trials.

    Usai, Alice / Di Franco, Gregorio / Colucci, Patrizia / Pollina, Luca Emanuele / Vasile, Enrico / Funel, Niccola / Palmeri, Matteo / Dente, Luciana / Falcone, Alfredo / Morelli, Luca / Raffa, Vittoria

    Cancers

    2020  Volume 12, Issue 3

    Abstract: Animal "avatars" and co-clinical trials are being developed for possible use in personalized medicine in oncology. In a co-clinical trial, the cancer cells of the patient's tumor are xenotransplanted into the animal avatar for drug efficacy studies, and ... ...

    Abstract Animal "avatars" and co-clinical trials are being developed for possible use in personalized medicine in oncology. In a co-clinical trial, the cancer cells of the patient's tumor are xenotransplanted into the animal avatar for drug efficacy studies, and the data collected in the animal trial are used to plan the best drug treatment in the patient trial. Zebrafish have recently been proposed for implementing avatar models, however the lack of a general criterion for the chemotherapy dose conversion from humans to fish is a limitation in terms of conducting co-clinical trials. Here, we validate a simple, reliant and cost-effective avatar model based on the use of zebrafish embryos. By crossing data from safety and efficacy studies, we found a basic formula for estimating the equivalent dose for use in co-clinical trials which we validated in a clinical study enrolling 24 adult patients with solid cancers (XenoZ, NCT03668418).
    Language English
    Publishing date 2020-03-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers12030677
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top