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Article ; Online: Genetic analysis of the invasive alga Didymosphenia geminata in Southern Argentina: Evidence of a Pleistocene origin of local lineages.

Jones, Leandro R / Manrique, Julieta M / Uyua, Noelia M / Whitton, Brian A

2019 Volume 9, Issue 1, Page(s) 18706

Abstract: The diatom Didymosphenia geminata has gained notoriety due to the massive growths which have occurred in recent decades in temperate regions. Different explanations have been proposed for this phenomenon, including the emergence of new invasive strains, ... ...

Abstract	The diatom Didymosphenia geminata has gained notoriety due to the massive growths which have occurred in recent decades in temperate regions. Different explanations have been proposed for this phenomenon, including the emergence of new invasive strains, human dispersion and climate change. Despite the fact in Argentina nuisance growths began in about 2010, historical records suggest that the alga was already present before that date. In addition, preliminary genetic data revealed too high a diversity to be explained by a recent invasion. Here, we estimate the divergence times of strains from southern Argentina. We integrate new genetic data and secondary, fossil and geological calibrations into a Penalized Likelihood model used to infer 18,630 plausible chronograms. These indicate that radiation of the lineages in Argentina began during or before the Pleistocene, which is hard to reconcile with the hypothesis that a new variant is responsible for the local mass growths. Instead, this suggests that important features of present distribution could be the result of multiple recent colonizations or the expansion of formerly rare populations. The text explains how these two possibilities are compatible with the hypothesis that recent nuisance blooms may be a consequence of climate change.
MeSH term(s)	Argentina ; Biological Evolution ; Climate Change ; DNA, Mitochondrial/genetics ; Diatoms/genetics ; Ecosystem ; Evolution, Molecular ; Genetic Testing ; Genetic Variation/genetics ; Introduced Species ; Phylogeny ; Rivers
Chemical Substances	DNA, Mitochondrial
Language	English
Publishing date	2019-12-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-55155-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: The peril of PCR inhibitors in environmental samples: the case of Didymosphenia geminata

Jones, Leandro R / Uyua, Noelia M / Manrique, Julieta M

Biodiversity and conservation. 2015 June, v. 24, no. 6

2015

Abstract: Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high ... ...

Abstract	Since the introduction of polymerase chain reaction (PCR) biodiversity study has been significantly influenced by the chance of generating unprecedented amounts of molecular data. Although it is a robust technique, those applications requiring high sensitivity and reproducibility, that is PCR detection and quantitative PCR, can be negatively affected by PCR inhibition. This is particularly challenging for diverse kinds of samples included the environmental ones, which usually contain complex mixtures of a variety of inhibitory substances. The problem of PCR inhibition can be overcome, or ameliorated, by implementing adequate protocols of nucleic acids purification, internal controls and modern analytical approaches focused on PCR kinetics. Herein, we remark these procedures and describe the general techniques that can be used to optimize DNA extraction protocols for PCR applications. In addition, we show that PCR inhibition might have negative consequences in molecular studies of Didymosphenia geminata, an invasive microalga that have recently developed massive blooms in temperate regions worldwide, and provide general guidelines for dealing with this problem.
Keywords	DNA ; biodiversity ; guidelines ; microalgae ; quantitative polymerase chain reaction ; temperate zones
Language	English
Dates of publication	2015-06
Size	p. 1541-1548.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	2000787-5
ISSN	1572-9710 ; 0960-3115
ISSN (online)	1572-9710
ISSN	0960-3115
DOI	10.1007/s10531-014-0849-5
Database	NAL-Catalogue (AGRICOLA)