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Article ; Online: Pharmacognostical and physicochemical evaluation on vata (ficus benghalensis linn.) Jata (Aerial Roots)

Pravin Jawanjal / C R Harisha / V J Shukla / B J Patgiri / S S Savrikar

Medical Journal of Dr. D.Y. Patil Vidyapeeth, Vol 15, Iss 1, Pp 86-

2022 Volume 92

Abstract: Introduction: Vata (Ficus benghalensis Linn.) is a sacred medicinal plant since Vedic times. It spread all over by its hanging or supporting roots, hence called as Vata. Aims: The aim was to study the macro- and microscopic characters, physiochemical and ...

Abstract	Introduction: Vata (Ficus benghalensis Linn.) is a sacred medicinal plant since Vedic times. It spread all over by its hanging or supporting roots, hence called as Vata. Aims: The aim was to study the macro- and microscopic characters, physiochemical and high-performance thin-layer chromatography (HPTLC) profile of Vata (F. benghalensis Linn.) Jata (aerial roots). Materials and Methods: Experiments were performed on authenticated plant materials, following standard procedures. Macroscopic and microscopic pharmacognostical characters of aerial roots and histochemical studies were noted. Results: Externally, the aerial root is gray and rough along with transverse rows of lenticels. Inner part of the aerial root is reddishbrown fibrous nature in bark portion. The powder microscopy study revealed the presence of fragment of simple fiber without lumen, stone cells with tannin, simple starch grain with hilum, compound starch grain, fiber with denim contents, latex contents, tannin contents rhomboidal crystal 2, rhomboidal crystal 3, group of stone cell with tannin, and fiber passing through medullary rays. Discussion: Transverse section of aerial root showed outer interesting exodermis followed by cork and cortex. Cortex region abundantly enriched by calcium oxalate crystal and tannin content are the diagnostic characters. Conclusion: Results of pharmacognostical and physiochemical analysis including HPTLC on aerial roots will help in further standardization.
Keywords	ficus benghalensis linn ; high-performance thin-layer chromatography ; moraceae ; pharmacognosy ; vata ; Medicine ; R
Subject code	580
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Wolters Kluwer Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Image processing and Ultra-Violet and Visible reflectance spectroscopy combined with chemometrics for discrimination as well as authentication powder and extract with anti- diabetic polyherbal formulation.

Jayanta Kumar Maji / Shikha Sharma / V.J.Shukla

International Journal of Pharma Sciences and Research , Vol 7, Iss 8, Pp 325-

2016 Volume 334

Abstract: The proposed anti-diabetic polyherbal formulation “Diabetogen” is composed of Indian Kino (Ht.wd.), Indian Liac (Lf.), Ram’s horn (Lf.), Fenugreek (Sd.), black berry (Sd.) and heart-leavedv moonseed (St.) is categorized antioxidant rich medicament and ... ...

Abstract	The proposed anti-diabetic polyherbal formulation “Diabetogen” is composed of Indian Kino (Ht.wd.), Indian Liac (Lf.), Ram’s horn (Lf.), Fenugreek (Sd.), black berry (Sd.) and heart-leavedv moonseed (St.) is categorized antioxidant rich medicament and has been clinically used in the Indian subcontinent to various permutation and combination. To establish a real time identification system in favor of non –destructive ultra-violet visible (UV-VIS) spectroscopy & preliminary image processing. The multivariate chemometrics technique principal component analysis (PCA), hierarchical cluster analysis (HCA) use and allow an overall evaluation of the significant difference between groups and discriminate the polyherbal powder and extract. The authenticated individual herbal, polyherbal pulverized powder and the dry extracts were both shifted through eighty mesh. The samples were subjected to UV-Vis diffuse reflectance spectral detection at the interval of 1 nm. The macroscopic image powders of herbal plants planning for plant identification, was carried out by LAB color based image segmentation. Plant powders macroscopic image shown distinct LAB color based segmentation for identifying sample. Samples discriminated by first-order derivative preprocessed reflectance spectra on favor of various transitions marker bands. Discrimination of the two classes of remedy was also able in natural grouping by PCA and HCA technique. An analytical method which is rapid, simple and accurate for discriminating two forms of polyherbal formulation using (UV-VIS) diffuse reflectance spectroscopy combined with well-known chemometrics method was developed along with powder image processing.
Keywords	Chemometrics ; Discrimination ; Diabetogen ; Image processing ; UV-VIS reflectance Spectroscopy. ; Pharmacy and materia medica ; RS1-441 ; Medicine ; R
Language	English
Publishing date	2016-08-01T00:00:00Z
Publisher	KEJA Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: PRELIMINARY PHARMACOGNOSTICAL AND PHYTOCHEMICAL EVALUATION OF AROGYAVARDHINI COMPOUND- AN EMERGING FORMULATED MEDICINE FOR METABOLIC SYNDROME

Bharatkumar Chhaganbhai Padhar / Alankruta R Dave / C R Harisha / V J Shukla

Journal of Drug Delivery and Therapeutics, Vol 6, Iss 1, Pp 46-

2016 Volume 50

Abstract: Context: Arogyavardhini Compound is an emerging formulated herbo-mineral formulation for treatment of metabolic syndrome. Metabolic syndrome is group of risk factors like, increased waist circumference, insulin resistance, increased triglycerides, ... ...

Abstract	Context: Arogyavardhini Compound is an emerging formulated herbo-mineral formulation for treatment of metabolic syndrome. Metabolic syndrome is group of risk factors like, increased waist circumference, insulin resistance, increased triglycerides, decreased high density lipoproteins and hypertension for coronary artery diseases and type 2 diabetes mellitus. Arogyavardhini compound consist equal quantity of Arogyavardhini Rasa and single bulb garlic powder. Arigyavardhini Rasa having proven anti dyslipidemiac and weight reducing effect and Garlic having antidiabetic, antidyslipidemic, antihypertensive effect, the combination called Arogyavardhini Compound has been formulated for management of metabolic syndrome. Aim: Authentication of raw drug of Arogyavardhini Compound and phytochemical evaluation of finished product. Materials and methods: Arogyavardhini Compound was evaluated on the basis of powder microscopy and analytical parameters like pH, Ash value, acid insoluble ash, water soluble extract, methanol extract and high performance thin layer chromatography. Results: Powder microscopy revealed the presence of Annular vessels of Musta, Starch grains of Vacha, Stone cells of Pippali, Stone cells of Chitraka, Oleoresins of Shunthi, etc. Physicochemical parameters such as total Ash value (15.91%), water soluble extract (13.5%), methanol soluble extract (17.2%) were assessed in preliminary physicochemical scanning. HPTLC revealed maximum 09 spots in short wave UV 254 nm. & 07 spots were obtained in long wave UV 366 nm. Conclusion: Pharmacognostical study revealed genuinity of raw drugs. Physico-chemical and HPTLC studies inferred that the formulation meets the minimum quality standards as reported in the API at a preliminary level. The inference from this study may be used as reference standard in the further quality control researches.
Keywords	Arogyavardhini Compound ; HPTLC ; Pharmacognosy ; Physicochemical analysis. ; Therapeutics. Psychotherapy ; RC475-489 ; Psychiatry ; RC435-571 ; Neurology. Diseases of the nervous system ; RC346-429 ; Neurosciences. Biological psychiatry. Neuropsychiatry ; RC321-571 ; Internal medicine ; RC31-1245 ; Medicine ; R
Subject code	630
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Society of Pharmaceutical Technocrats
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.

Nayan R Bhalodia / V J Shukla

Journal of Advanced Pharmaceutical Technology & Research, Vol 2, Iss 2, Pp 104-

An ethnomedicinal plant

2011 Volume 109

Abstract: This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on ... ...

Abstract	This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of Cassia fistula Linn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important bacterial and fungal strains. The antimicrobial activity was determined in the extracts using agar disc diffusion method. The antibacterial and antifungal activities of extracts (5, 25, 50, 100, 250 ΅g/ml) of Cassia fistula were tested against two Gram-positive--Staphylococcus aureus, Streptococcus pyogenes; two Gram-negative--Escherichia coli, Pseudomonas aeruginosa human pathogenic bacteria; and three fungal strains--Aspergillus niger, Aspergillus clavatus, Candida albicans. Zone of inhibition of extracts were compared with that of different standards like ampicillin, ciprofloxacin, norfloxacin, and chloramphenicol for antibacterial activity and nystatin and griseofulvin for antifungal activity. The results showed that the remarkable inhibition of the bacterial growth was shown against the tested organisms. The phytochemical analyses of the plants were carried out. The microbial activity of the Cassia fistula was due to the presence of various secondary metabolites. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals research activities.
Keywords	Cassia fistula ; in vitro antibacterial activity ; antifungal activity ; secondary metabolites ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
Publishing date	2011-01-01T00:00:00Z
Publisher	Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Quality control evaluation of Guduchi Satva (solid aqueous extract of Tinospora cordifolia (Willd.) Miers)

Rohit Sharma / Hetal Amin / V J Shukla / Dhiman Kartar / R Galib / P K Prajapati

International Journal of Green Pharmacy, Vol 7, Iss 3, Pp 258-

An herbal formulation

2013 Volume 263

Abstract: Introduction: Herbal products represent complex biological mixture and achieving a reproducible pharmaceutical quality could be a very challenging task in standardisation of herbal products. Hence, methodical approach is required for standardisation, ... ...

Abstract	Introduction: Herbal products represent complex biological mixture and achieving a reproducible pharmaceutical quality could be a very challenging task in standardisation of herbal products. Hence, methodical approach is required for standardisation, development of sufficient quality control profile and scientific validation of plant extracts, as is done in contemporary medicine. Ayurvedic physicians prescribe dried extract of Tinospora cordifolia (Willd.) Miers known as ′Guduchi Satva′ in several febrile conditions. However, till today no published information is available on its analytical profile. Aims and Objectives: Present study is aimed to evaluate quality control parameters for Guduchi Satva. Materials and Methods: Fifteen batches of Guduchi Satva were prepared and findings were systematically recorded. Physicochemical parameters and qualitative tests for various functional groups, quantitative estimation of total alkaloids, fluorescence analysis, high performance thin layer chromatography (HPTLC) profile, heavy metal analysis and microbial load of Guduchi Satva were carried out. Results: The average percentage of dried Satva obtained was 3.8%. Alkaloids, carbohydrates and starch were found present in Satva. Number of peaks obtained in HPTLC also corresponds to this finding. Percentage of total alkaloid content was 0.31%. No heavy metal and microbial load were detected in the sample. The present observation can be considered as standard for further studies on Satva.
Keywords	Guduchi ; quality control ; Satva ; Tinospora cordifolia ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
Language	English
Publishing date	2013-01-01T00:00:00Z
Publisher	Medknow Publications Pvt Ltd
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Pharmacognostical and phytochemical evaluation of Tryoshnadi Guggulu Vati

Bharat Chhaganbhai Padhar / Alankruta R Dave / Mandip R Goyal / C R Harisha / V J Shukla / Dinesh B Khant

International Journal of Green Pharmacy, Vol 9, Iss 3, Pp 150-

An ayurvedic polyherbal formulation

2015 Volume 156

Abstract: Introduction: Tryoshnadi Guggulu Vati, a polyherbal formulation is recommended for the management of diseases due to Medodushti (abnormal fat metabolism) like obesity, diabetes mellitus, dyslipidemia, coronary artery diseases, etc. Though Tryoshnadi ... ...

Abstract	Introduction: Tryoshnadi Guggulu Vati, a polyherbal formulation is recommended for the management of diseases due to Medodushti (abnormal fat metabolism) like obesity, diabetes mellitus, dyslipidemia, coronary artery diseases, etc. Though Tryoshnadi Guggulu Vati is widely used for the treatment of diseases due to abnormal metabolism, but till date, its pharmacognostical and pharmaceutical study has not been carried out. Aim: Authentication of raw drug of Tryoshnadi Guggulu Vati and phytochemical evaluation of finished product. Materials and Methods: The present study deals with the pharmacognostical identification of the ingredients of Tryoshnadi Guggulu Vati and its physicochemical analysis. Results: Powder microscopy revealed the presence of annular vessels of Musta, starch grains of Vacha, stone cells of Pippali, stone cells of Chitraka, oleoresins of Shunthi, starch grains of Musta, etc., Physicochemical parameters such as total ash value (15.91%), water soluble extract (13.5%), methanol soluble extract (17.2%) were assessed in preliminary physicochemical scanning. High-performance thin layer chromatography (HPTLC) revealed maximum 10 spots in short wave ultraviolet (UV) 254 nm. And four spots were obtained in long wave UV 366 nm. Conclusion: Pharmacognostical study revealed genuinity of raw drugs. Physicochemical and HPTLC studies inferred that the formulation meets the minimum quality standards as reported in the American Petroleum Institute at a preliminary level. The inference from this study may be used as reference standard in the further quality control researches.
Keywords	High-performance thin layer chromatography ; pharmacognosy ; physicochemical analysis ; Tryoshnadi Guggulu Vati ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
Language	English
Publishing date	2015-01-01T00:00:00Z
Publisher	Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Pharmacognostical and pharmaceutical assay of Asanadi Ghanavati

Shivam Joshi / H M Chandola / C R Harisha / V J Shukla

International Journal of Green Pharmacy, Vol 6, Iss 2, Pp 151-

An Ayurvedic polyherbal formulation

2012 Volume 156

Abstract: Context: Ayurveda has abundant collection of effective formulations against diseases. In the era of increasing demand for indigenous medicines, maintaining quality standards is the need of the hour. Standardisation of compound formulations is lagging ... ...

Abstract	Context: Ayurveda has abundant collection of effective formulations against diseases. In the era of increasing demand for indigenous medicines, maintaining quality standards is the need of the hour. Standardisation of compound formulations is lagging behind because of absence of reference standards. Lots of single and compound drugs have been described in Ayurvedic classics. Asanadi gana (Astanga Hridaya Sutrasthana 15) has kapha-medohara (pacifying Kapha and fat) and lekhana (scraping) property. Aims: The present study was aimed at setting a standard pharmacognostical and pharmaceutical profile of Asanadi ghanavati (AG). Materials and Methods: Study included preparation of AG following all SOPs using raw drugs, which were previously authenticated. Later, AG was subjected to pharmacognostical, physicochemical and high performance thin-layer chromatography (HPTLC) analysis as per standard protocols. Results: The final observations were recorded. Pharmacognostical findings matched with that of individual raw drugs with no major change in the microscopic structure of the raw drugs during preparation of ghanavati. HPTLC gave the fingerprint of the formulation with 12 and eight spots on short and long UV, respectively. Conclusions : The quality of AG tablet can be tested by series of pharmacognostical, Physicochemical screening for the observations of the present study.
Keywords	Asanadi ghanavati ; dyslipidaemia ; HPTLC ; pharmacognosy ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Medknow Publications Pvt Ltd
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Pharmacognostical and physicochemical analysis of Tamarindus indica Linn. stem

Naveena Kodlady / B J Patgiri / C R Harisha / V J Shukla

Journal of Ayurveda and Integrative Medicine, Vol 3, Iss 1, Pp 6-

2012 Volume 9

Abstract: Tamarindus indica Linn. fruits (Chincha) are extensively used in culinary preparations in Indian civilization. Its vast medicinal uses are documented in Ayurvedic classics and it can be used singly or as a component of various formulations. Besides fruit, ...

Abstract	Tamarindus indica Linn. fruits (Chincha) are extensively used in culinary preparations in Indian civilization. Its vast medicinal uses are documented in Ayurvedic classics and it can be used singly or as a component of various formulations. Besides fruit, the Kasta (wood) of T. indica L. is also important and used to prepare Kshara (alkaline extract) an Ayurvedic dosage form. Pharmacognostical and physicochemical details of Chincha Kasta are not available in authentic literature including API (Ayurvedic Pharmacopoeia of India). The study is an attempt in this direction. T. indica L. stem with heartwood was selected and morphological, microscopic and physicochemical standardization characters along with TLC finger print, and fluorescence analysis were documented. Transverse section of stem showed important characters such as phelloderm, stone cells layer, fiber groups, calcium oxalate, crystal fibers, and tylosis in heartwood region. Four characteristic spots were observed under UV long wave, in thin layer chromatography with the solvent combination of toluene: ethyl acetate (8:2). The study can help correct identification and standardization of this plant material.
Keywords	Ayurveda ; Chincha ; powder microscopy ; tamarind ; thin layer chromatography ; Medicine (General) ; R5-920 ; Medicine ; R
Subject code	571
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A study to evaluate bacoside A in Brahmi Ghrita by HPTLC method

Jyoti S Gubbannavar / H M Chandola / Renuka Kalyani / V J Shukla

International Journal of Green Pharmacy, Vol 6, Iss 3, Pp 184-

2012 Volume 186

Abstract: Context: Brahmi (Bacopa monnieri Linn. Pennel), the main ingredient of Brahmi Ghrita is a popular Medhya drug referred in Ayurvedic classics. Recent advances have established the action of bacoside, one of the constituents of Brahmi in various psycho- ... ...

Abstract	Context: Brahmi (Bacopa monnieri Linn. Pennel), the main ingredient of Brahmi Ghrita is a popular Medhya drug referred in Ayurvedic classics. Recent advances have established the action of bacoside, one of the constituents of Brahmi in various psycho-neurological disorders. Aim: Present study was planned to detect bacoside A and to do quantitative and qualitative analysis of bacoside A in Brahmi Ghrita using standard bacoside A sample through HPTLC method. Materials and Methods: Stationary phase was precoated silica gel GF 254 and mobile phase was dichloromethane: methanol: water (4.5:1.0:0.1 v/v/v). The plate was scanned and quantified at 254 nm for bacoside A. Result: The correlation co-efficient of bacoside A was found to be 0.9989 or 20% in 5 μl Brahmi Ghrita sample. Conclusion: The study confirms the presence of bacoside A in Brahmi Ghrita. The correlation coefficient of bacoside A was found to be 0.9989 or 20% in 5 μl Brahmi Ghrita sample. The simple, accurate and cost-effective HPTLC method can be utilized for the routine analysis of quantitative determination of bacoside A in Brahmi Ghrita.
Keywords	Bacoside A ; Brahmi Ghrita ; HPTLC ; Therapeutics. Pharmacology ; RM1-950 ; Medicine ; R
Language	English
Publishing date	2012-01-01T00:00:00Z
Publisher	Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparison of gastroprotective effects of triphala formulations on stress-induced ulcer in rats

M B Nariya / V J Shukla / B Ravishankar / S M Jain

Indian Journal of Pharmaceutical Sciences, Vol 73, Iss 6, Pp 682-

2011 Volume 687

Abstract: Triphala is categorized as rejuvenator and traditionally been used in various gastric disorders including intestinal inflammation. The aim of present study was to examine the comparative gastroprotective effects of Triphala formulations against ... ...

Abstract	Triphala is categorized as rejuvenator and traditionally been used in various gastric disorders including intestinal inflammation. The aim of present study was to examine the comparative gastroprotective effects of Triphala formulations against experimental gastric ulcer in rats to substantiate its traditional claim. Gastric ulcer was induced by water immersion plus stress-induced ulcers in rats. The drug effects were assessed by studying macroscopic gross injury and stomach tissue biochemical parameters. Triphala unequal formulation and Chinnodbhavadi kwath showed significant antiulcer activity and this is evident from reduction of ulcer index, lipid peroxidation and hydroxyl radical levels and concomitantly raised levels of catalase and superoxide dismutase. Though similar kind of activity was observed in Triphala equal formulation the magnitude was much less. Further, Chinnodbhavadi kwath significantly increased the glutathione and ATPase level but Triphala equal formulation significantly increased glutathione level only. Based on the data generated, it is suggested that among the three formulations studied, Chinnodbhavadi kwath and Triphala unequal formulations provides significant protection in gastric ulcer as compared to Triphala equal formulation.
Keywords	Chinnodbhavadi kwath ; gastroprotection ; rejuvenator ; Triphala ; Pharmacy and materia medica ; RS1-441 ; Medicine ; R
Subject code	571
Language	English
Publishing date	2011-01-01T00:00:00Z
Publisher	Medknow Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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