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Article ; Online: Glyphosate modifies the gene expression and migration of trophoblastic cells without altering the process of angiogenesis or the implantation of blastocysts in vitro.

Oddi, Sofía / Altamirano, Gabriela A / Zenclussen, María L / Abud, Julián E / Vaira, Stella / Gomez, Ayelen L / Schierano-Marotti, Gonzalo / Muñoz-de-Toro, Mónica / Kass, Laura

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2024 , Page(s) 114748

Abstract: Adverse pregnancy outcomes have been associated with the presence of glyphosate (G) in umbilical cord, serum, and urine samples from pregnant women. Our aim was to study the effect of G on blastocyst implantation using an in vitro mouse model, and the ... ...

Abstract	Adverse pregnancy outcomes have been associated with the presence of glyphosate (G) in umbilical cord, serum, and urine samples from pregnant women. Our aim was to study the effect of G on blastocyst implantation using an in vitro mouse model, and the migration and acquisition of endothelial phenotype of the human trophoblastic HTR8/SVneo (H8) cells. In mouse blastocysts, no differences in attachment time and implantation outgrowth area were observed after G exposure. H8 cell migration was stimulated by 0.625 μM G without cytotoxicity. After 6 h, the mRNA expression of vascular endothelial growth factor (VEGF) and C-C motif chemokine ligand 2 (CCL2) was upregulated in H8 cells exposed to 1.25 μM G when compared vehicle-treated cells (p ≤0.05). No differences were observed in interleukin 11, VEGF receptor 1, and coagulation factor II thrombin receptor in H8 cells exposed to different concentrations of G for 6 h compared to the vehicle. Interestingly, exposure to G did not alter angiogenesis as measured by a tube formation assay. Taken all together, these results suggest that G exposure may contribute as a risk factor during pregnancy, due to its ability to alter trophoblast migration and gene expression.
Language	English
Publishing date	2024-05-17
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2024.114748
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Article: Adulteration of Argentinean milk fats with animal fats: Detection by fatty acids analysis and multivariate regression techniques.

Rebechi, S R / Vélez, M A / Vaira, S / Perotti, M C

Food chemistry

2016 Volume 192, Page(s) 1025–1032

Abstract: The aims of the present study were to test the accuracy of the fatty acid ratios established by the Argentinean Legislation to detect adulterations of milk fat with animal fats and to propose a regression model suitable to evaluate these adulterations. ... ...

Abstract	The aims of the present study were to test the accuracy of the fatty acid ratios established by the Argentinean Legislation to detect adulterations of milk fat with animal fats and to propose a regression model suitable to evaluate these adulterations. For this purpose, 70 milk fat, 10 tallow and 7 lard fat samples were collected and analyzed by gas chromatography. Data was utilized to simulate arithmetically adulterated milk fat samples at 0%, 2%, 5%, 10% and 15%, for both animal fats. The fatty acids ratios failed to distinguish adulterated milk fats containing less than 15% of tallow or lard. For each adulterant, Multiple Linear Regression (MLR) was applied, and a model was chosen and validated. For that, calibration and validation matrices were constructed employing genuine and adulterated milk fat samples. The models were able to detect adulterations of milk fat at levels greater than 10% for tallow and 5% for lard.
MeSH term(s)	Animals ; Argentina ; Chromatography, Gas/methods ; Dietary Fats/analysis ; Fats/analysis ; Fatty Acids/analysis ; Food Contamination/analysis ; Milk/chemistry ; Multivariate Analysis ; Regression Analysis ; Reproducibility of Results
Chemical Substances	Dietary Fats ; Fats ; Fatty Acids ; tallow (98HPY76U4W) ; lard (SI6O3IW77Z)
Language	English
Publishing date	2016-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2015.07.107
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Article ; Online: Correction to: Response to comments on: Perinatal exposure to a glyphosate-based herbicide impairs female reproductive outcomes and induces second-generation adverse effects in Wistar rats.

Milesi, María M / Lorenz, Virginia / Beldomenico, Pablo M / Vaira, Stella / Varayoud, Jorgelina / Luque, Enrique H

Archives of toxicology

2020 Volume 94, Issue 8, Page(s) 2897–2898

Abstract: We report a mistake in the tables published in Milesi et al. (2019). ...

Abstract	We report a mistake in the tables published in Milesi et al. (2019).
Language	English
Publishing date	2020-06-24
Publishing country	Germany
Document type	Journal Article ; Published Erratum
ZDB-ID	124992-7
ISSN	1432-0738 ; 0340-5761
ISSN (online)	1432-0738
ISSN	0340-5761
DOI	10.1007/s00204-020-02814-2
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Article ; Online: Response to comments on: Perinatal exposure to a glyphosate-based herbicide impairs female reproductive outcomes and induces second-generation adverse effects in Wistar rats.

Milesi, María M / Lorenz, Virginia / Beldomenico, Pablo M / Vaira, Stella / Varayoud, Jorgelina / Luque, Enrique H

Archives of toxicology

2019 Volume 93, Issue 12, Page(s) 3635–3638

MeSH term(s)	Animals ; Female ; Glycine/analogs & derivatives ; Herbicides ; Pregnancy ; Rats ; Rats, Wistar ; Reproduction ; Glyphosate
Chemical Substances	Herbicides ; Glycine (TE7660XO1C)
Language	English
Publishing date	2019-11-13
Publishing country	Germany
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	124992-7
ISSN	1432-0738 ; 0340-5761
ISSN (online)	1432-0738
ISSN	0340-5761
DOI	10.1007/s00204-019-02609-0
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Article ; Online: Wnt/β-catenin signaling pathway and thioredoxin-interacting protein (TXNIP) mediate the "glucose sensor" mechanism in metastatic breast cancer-derived cells MDA-MB-231.

Vaira, Sergio / Friday, Ellen / Scott, Keith / Conrad, Steven / Turturro, Francesco

Journal of cellular physiology

2012 Volume 227, Issue 2, Page(s) 578–586

Abstract: In this study we investigated the effect of glucose on GSK3β and β-catenin expression and the involvement of the N-linked glycosylation and hexosamine pathways in the Wnt canonical pathway in response to in vitro conditions resembling normoglycemia (5 ... ...

Abstract	In this study we investigated the effect of glucose on GSK3β and β-catenin expression and the involvement of the N-linked glycosylation and hexosamine pathways in the Wnt canonical pathway in response to in vitro conditions resembling normoglycemia (5 mmol) and hyperglycemia (20 mmol) in the metastatic breast cancer-derived cell line MDA-MB-231. We also investigated the relationship between this circuitry and the thioredoxin-interacting protein (TXNIP) regulation that seems to be related. MDA-MB-231 cells were grown either in 5 or 20 mM glucose chronically prior to plating. For glucose shift (5/20), cells were plated in 5 mM glucose and shifted to 20 mM at time 0. Both protein and mRNA levels for GSK3β but only the protein expression for β-catenin, were increased in response to high glucose. Furthermore, we assessed the response of GSK3β, β-catenin, and TXNIP to inhibition of the N-linked glycosylation, hexosamine, and Wnt pathways. Wnt signaling pathway activation was validated by specific reporter assay. We show that high levels of glucose regulate mRNA and protein expression of GSK3β, and consequently higher levels of activated β-catenin protein, which locates to the nucleus and is associated with increased levels of cyclin D1 expression. This event coincides with increased level of N-terminal Ser 9 phosphorylation of GSK3β protein. The inhibition of both the hexosamine pathway and N-linked glycosylation along with Wnt signaling pathway by sFRP1 and DKK1 is associated with significant decrease of the protein levels of GSK3β, β-catenin, and TXNIP RNA. Our work illuminates a novel and never described before function of this signaling pathway that relates glucose metabolism with redox regulation mechanism.
MeSH term(s)	Breast Neoplasms/metabolism ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cell Line, Tumor ; Cyclin D1/genetics ; Cyclin D1/metabolism ; Female ; Gene Expression Regulation, Neoplastic/physiology ; Glucose/metabolism ; Glycogen Synthase Kinase 3/genetics ; Glycogen Synthase Kinase 3/metabolism ; Glycogen Synthase Kinase 3 beta ; Humans ; Signal Transduction/physiology ; Wnt Proteins/genetics ; Wnt Proteins/metabolism ; beta Catenin/genetics ; beta Catenin/metabolism
Chemical Substances	Carrier Proteins ; TXNIP protein, human ; Wnt Proteins ; beta Catenin ; Cyclin D1 (136601-57-5) ; GSK3B protein, human (EC 2.7.11.1) ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; Glycogen Synthase Kinase 3 (EC 2.7.11.26) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2012-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.22757
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Article: Acción de distintos coagulantes para la eliminación de Cryptosporidium spp. en el proceso de potabilización del agua.

Abramovich, B / Lura, M C / Carrera, E / Gilli, M I / Haye, M A / Vaira, S

Revista Argentina de microbiologia

2004 Volume 36, Issue 2, Page(s) 92–96

Abstract: Cryptosporidium is one of the microorganisms of main concern from the point of view of Public Health, being a priority problem for water treatment plants and water regulatory institutions. Due to its small size and resistance to chlorination, ... ...

Title translation	The action of different coagulants to remove Cryptosporidium during the process of water treatment.
Abstract	Cryptosporidium is one of the microorganisms of main concern from the point of view of Public Health, being a priority problem for water treatment plants and water regulatory institutions. Due to its small size and resistance to chlorination, Cryptosporidium removal during the process of drinking water treatment is a hard task. The effectiveness of different coagulants commonly used in the process of removal of oocysts was analyzed. The technique used was the Jar Test. It was found that: 1) coagulants with the addition of polimeric coadjuvants produce over 2 logs of oocyst removal; 2) a low value in turbidity does not necessarily mean optimal parasite removal, and 3) the addition of polyelectrolites to ferric chloride diminishes variability, both in final turbidity and Cryptosporidium removal.
MeSH term(s)	Animals ; Cryptosporidiosis/prevention & control ; Cryptosporidiosis/transmission ; Cryptosporidium ; Humans ; Oocysts ; Water Microbiology ; Water Purification/methods
Language	Spanish
Publishing date	2004-04
Publishing country	Argentina
Document type	English Abstract ; Journal Article
ZDB-ID	731952-6
ISSN	0325-7541
ISSN	0325-7541
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Article: Overexpression of twisted gastrulation inhibits bone morphogenetic protein action and prevents osteoblast cell differentiation in vitro.

Gazzerro, Elisabetta / Deregowski, Valerie / Vaira, Sergio / Canalis, Ernesto

Endocrinology

2005 Volume 146, Issue 9, Page(s) 3875–3882

Abstract: Twisted gastrulation (Tsg) is a secreted glycoprotein that binds bone morphogenetic protein-2 (BMP-2) and BMP-4 and can display both BMP agonist and antagonist functions. Tsg acts as a BMP agonist in chondrocytes, but its expression and actions on the ... ...

Abstract	Twisted gastrulation (Tsg) is a secreted glycoprotein that binds bone morphogenetic protein-2 (BMP-2) and BMP-4 and can display both BMP agonist and antagonist functions. Tsg acts as a BMP agonist in chondrocytes, but its expression and actions on the differentiation of cells of the osteoblastic lineage are not known. We investigated the effects of Tsg overexpression by transducing murine ST-2 stromal and MC3T3 cells with a retroviral vector where Tsg is under control of the cytomegalovirus promoter and compared them to cells transduced with the parental vector alone. ST-2 cells were cultured in osteoblastic differentiating conditions in the presence or absence of BMP-2. Tsg overexpression precluded the appearance of mineralized nodules induced by BMP-2, led to a delay in the expression of osteoblastic gene markers, and decreased the responsiveness of ST-2 differentiating cells to PTH. BMP-2 induced the phosphorylation of signaling mothers against decapentaplegic-1/5/8, but not ERK, c-Jun N-terminal kinase, and p38. ST-2 cells overexpressing Tsg displayed an inhibition of BMP/signaling mother against decapentaplegic signaling. Tsg action was specific to BMP, because Tsg overexpression did not affect TGF-beta or Wnt/beta-catenin signaling pathways. Tsg also opposed MC3T3 cell differentiation and the expression of a mature osteoblast phenotype. In conclusion, Tsg overexpression inhibits BMP action in stromal and preosteoblastic cells and, accordingly, arrests their differentiation toward the osteoblastic pathway.
MeSH term(s)	Animals ; Bone Marrow Cells/cytology ; Bone Marrow Cells/physiology ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins/metabolism ; Cell Differentiation/physiology ; Cells, Cultured ; Cytomegalovirus/genetics ; Gene Expression ; Genetic Vectors ; In Vitro Techniques ; Mice ; Osteoblasts/cytology ; Osteoblasts/physiology ; Phenotype ; Promoter Regions, Genetic ; Proteins/genetics ; Retroviridae/genetics ; Stromal Cells/cytology ; Stromal Cells/physiology ; Transforming Growth Factor beta/metabolism
Chemical Substances	Bmp2 protein, mouse ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins ; Proteins ; Transforming Growth Factor beta ; twisted gastrulation protein, mouse
Language	English
Publishing date	2005-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	427856-2
ISSN	1945-7170 ; 0013-7227
ISSN (online)	1945-7170
ISSN	0013-7227
DOI	10.1210/en.2005-0053
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Article ; Online: Creation and preliminary characterization of a leptin knockout rat.

Vaira, Sergio / Yang, Chang / McCoy, Aaron / Keys, Kelly / Xue, Shurong / Weinstein, Edward J / Novack, Deborah V / Cui, Xiaoxia

Endocrinology

2012 Volume 153, Issue 11, Page(s) 5622–5628

Abstract: Leptin, a cytokine-like hormone secreted mainly by adipocytes, regulates various pathways centered on food intake and energy expenditure, including insulin sensitivity, fertility, immune system, and bone metabolism. Here, using zinc finger nuclease ... ...

Abstract	Leptin, a cytokine-like hormone secreted mainly by adipocytes, regulates various pathways centered on food intake and energy expenditure, including insulin sensitivity, fertility, immune system, and bone metabolism. Here, using zinc finger nuclease technology, we created the first leptin knockout rat. Homozygous leptin null rats are obese with significantly higher serum cholesterol, triglyceride, and insulin levels than wild-type controls. Neither gender produced offspring despite of repeated attempts. The leptin knockout rats also have depressed immune system. In addition, examination by microcomputed tomography of the femurs of the leptin null rats shows a significant increase in both trabecular bone mineral density and bone volume of the femur compared with wild-type littermates. Our model should be useful for many different fields of studies, such as obesity, diabetes, and bone metabolism-related illnesses.
MeSH term(s)	Animals ; Body Weight/genetics ; Bone Density/genetics ; Cholesterol/blood ; Eating/genetics ; Energy Metabolism/genetics ; Femur/metabolism ; Insulin/blood ; Leptin/genetics ; Leptin/metabolism ; Obesity/genetics ; Obesity/metabolism ; Phenotype ; Rats ; Rats, Transgenic ; Triglycerides/blood ; Zinc Fingers
Chemical Substances	Insulin ; Leptin ; Triglycerides ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2012-09-04
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	427856-2
ISSN	1945-7170 ; 0013-7227
ISSN (online)	1945-7170
ISSN	0013-7227
DOI	10.1210/en.2012-1462
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Article: RelA/p65 promotes osteoclast differentiation by blocking a RANKL-induced apoptotic JNK pathway in mice.

Vaira, Sergio / Alhawagri, Muhammad / Anwisye, Imani / Kitaura, Hideki / Faccio, Roberta / Novack, Deborah Veis

The Journal of clinical investigation

2008 Volume 118, Issue 6, Page(s) 2088–2097

Abstract: Osteoclasts (OCs) function to reabsorb bone and are responsible for the bone loss associated with inflammatory arthritis and osteoporosis. OC numbers are elevated in most disorders of accelerated bone destruction, reflecting altered rates of precursor ... ...

Abstract	Osteoclasts (OCs) function to reabsorb bone and are responsible for the bone loss associated with inflammatory arthritis and osteoporosis. OC numbers are elevated in most disorders of accelerated bone destruction, reflecting altered rates of precursor differentiation and apoptosis. Both of these processes are regulated by the JNK family of MAP kinases. In this study, we have demonstrated that the NF-kappaB subunit RelA/p65 inhibits JNK-mediated apoptosis during a critical period of commitment to the OC phenotype in response to the cytokine RANKL. This RelA/p65-mediated arrest of cell death led to enhanced OC differentiation. Hence, Rela-/- OC precursors displayed prolonged JNK activation in response to RANKL, and this was accompanied by an increase in cell death that prevented efficient differentiation. Although complete blockade of JNK activity inhibits osteoclastogenesis, both short-term blockade in RelA-deficient cultures and suppression of the downstream mediator, Bid rescued apoptosis and differentiation. These antiapoptotic effects were RelA specific, as overexpression of RelA, but not RelB, blocked apoptosis and rescued differentiation in Rela-/- precursors. Thus, RelA blocks a RANKL-induced, apoptotic JNK-Bid pathway, thereby promoting OC differentiation. Consistent with this, mice lacking RelA/p65 in the hematopoietic compartment were shown to have a deficient osteoclastogenic response to RANKL and were protected from arthritis-induced osteolysis.
MeSH term(s)	Animals ; Apoptosis ; BH3 Interacting Domain Death Agonist Protein/metabolism ; Bone Marrow Cells/metabolism ; Caspases/metabolism ; Cell Differentiation ; Gene Expression Regulation ; JNK Mitogen-Activated Protein Kinases/metabolism ; MAP Kinase Kinase 4/metabolism ; Mice ; Mice, Transgenic ; Models, Biological ; Osteoclasts/metabolism ; RANK Ligand/metabolism ; Transcription Factor RelA/biosynthesis ; Transcription Factor RelA/physiology
Chemical Substances	BH3 Interacting Domain Death Agonist Protein ; Bid protein, mouse ; RANK Ligand ; Rela protein, mouse ; Transcription Factor RelA ; JNK Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; MAP Kinase Kinase 4 (EC 2.7.12.2) ; Caspases (EC 3.4.22.-)
Language	English
Publishing date	2008-05-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	3067-3
ISSN	1558-8238 ; 0021-9738
ISSN (online)	1558-8238
ISSN	0021-9738
DOI	10.1172/JCI33392
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Article: HGF and M-CSF modulate adhesion of MDA-231 breast cancer cell by increasing osteopontin secretion.

Grano, M / Mori, G / Minielli, V / Colucci, S / Vaira, S / Giannelli, G / Martemucci, S / Giorgino, F / Zallone, A Z

Journal of biological regulators and homeostatic agents

2002 Volume 16, Issue 3, Page(s) 190–195

Abstract: Recent studies reported an increased expression of osteopontin (OPN) in metastatic breast cancer cells, but the mechanisms modulating OPN production and the interaction of the cells with the secreted protein are far from clear. In this work, we utilized ... ...

Abstract	Recent studies reported an increased expression of osteopontin (OPN) in metastatic breast cancer cells, but the mechanisms modulating OPN production and the interaction of the cells with the secreted protein are far from clear. In this work, we utilized as an experimental system the cell line MDA-231 and we showed that HGF and M-CSF significantly enhance their adhesion onto OPN. Furthermore, in the presence of HGF and M-CSF, MDA-231 cells can adhere when plated onto BSA via increased OPN secretion. Moreover HGF and M-CSF induce de novo synthesis of OPN. In conclusion, these data suggest that HGF and M-CSF stimulate OPN production by MDA-231 cells, and that OPN is subsequently used as a substrate for cell adhesion.
MeSH term(s)	Blotting, Western ; Bone Matrix/metabolism ; Cell Adhesion ; Hepatocyte Growth Factor/physiology ; Humans ; Macrophage Colony-Stimulating Factor/physiology ; Osteopontin ; RNA, Messenger/metabolism ; Recombinant Proteins/metabolism ; Sialoglycoproteins/secretion ; Time Factors ; Tumor Cells, Cultured
Chemical Substances	RNA, Messenger ; Recombinant Proteins ; SPP1 protein, human ; Sialoglycoproteins ; Osteopontin (106441-73-0) ; Hepatocyte Growth Factor (67256-21-7) ; Macrophage Colony-Stimulating Factor (81627-83-0)
Language	English
Publishing date	2002-07
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639196-5
ISSN	1724-6083 ; 0393-974X
ISSN (online)	1724-6083
ISSN	0393-974X
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