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Article: Carbonated Irrigation Assessment of Grapevine Growth, Nutrient Absorption, and Sugar Accumulation in a Tempranillo (Vitis vinifera L.) Vineyard

Lampreave, Miriam / Mateos, Assumpta / Valls, Josep / Nadal, Montserrat / Sánchez-Ortiz, Antoni

Agriculture (Basel). 2022 May 30, v. 12, no. 6

2022

Abstract: Iron chlorosis is common in vineyards grown on calcareous soils, and consists of a chlorophyll deficiency caused by a disorder in iron nutrition. It is manifested by interveinal yellowing of the youngest leaves and decreased growth and production. Most ... ...

Abstract	Iron chlorosis is common in vineyards grown on calcareous soils, and consists of a chlorophyll deficiency caused by a disorder in iron nutrition. It is manifested by interveinal yellowing of the youngest leaves and decreased growth and production. Most of the vineyards in Spain are planted on calcareous soils, so iron chlorosis is frequent, even in tolerant rootstocks. In the case of Spain, Tempranillo accounts for 20.8% of the total Spanish wine area, with this red variety being the most planted. The study of iron chlorosis and the availability of nutrients is essential for improving the qualitative balance of the wine, as it is one of the most representative in our country. The aim of this work was to evaluate how carbonated irrigation modifies soil pH and increases the absorption of nutrients, as well as to assess the impact of chlorosis on the physiology of the variety and the composition of the grape. In the first instance, a test was carried out on bare soil of calcareous composition, evaluating three different levels of carbonated water, and seeing what response it gave to the pH level. The result showed that the dose of 400 ppm of CO₂ provided the optimal pH. Subsequently, in a potted soil, the dose of 400 ppm of CO₂ was evaluated on the variety cv. Tempranillo, in three different compositions of calcareous soil. Results showed that carbonated irrigation increased the levels of chlorophyll impacting on primary metabolism (acids and sugar), plant growth, and higher crop yield, improving the optimal grape ripeness. Given that irrigation with 400 ppm CO₂ increased the ability to control ferric chlorosis, this strategy could be an easy-to-use alternative to iron-based chelates for preventing Fe deficiency in the grapevine as well as moderating the different levels of iron chlorosis. This strategy could be an alternative to the use of synthetic Fe chelates as EDTA or o,o-EDDHA for preventing Fe chlorosis in susceptible Tempranillo (Vitis vinifera L.) vineyard in calcareous soils, with less of a risk to the environment.
Keywords	Vitis vinifera ; absorption ; agriculture ; calcareous soils ; carbon dioxide ; chlorophyll ; chlorosis ; crop yield ; grapes ; irrigation ; metabolism ; nutrient deficiencies ; nutrient uptake ; plant growth ; risk ; soil pH ; sugars ; vineyards ; wines ; Spain
Language	English
Dates of publication	2022-0530
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2651678-0
ISSN	2077-0472
ISSN	2077-0472
DOI	10.3390/agriculture12060792
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Elicitor-Induced VOC Emission by Grapevine Leaves: Characterisation in the Vineyard.

Lemaitre-Guillier, Christelle / Chartier, Agnès / Dufresne, Christelle / Douillet, Antonin / Cluzet, Stéphanie / Valls, Josep / Aveline, Nicolas / Daire, Xavier / Adrian, Marielle

Molecules (Basel, Switzerland)

2022 Volume 27, Issue 18

Abstract: The present study is aimed at determining whether leaf volatile organic compounds (VOCs) are good markers of the grapevine response to defence elicitors in the field. It was carried out in two distinct French vineyards (Burgundy and Bordeaux) over 3 ... ...

Abstract	The present study is aimed at determining whether leaf volatile organic compounds (VOCs) are good markers of the grapevine response to defence elicitors in the field. It was carried out in two distinct French vineyards (Burgundy and Bordeaux) over 3 years. The commercial elicitor Bastid
MeSH term(s)	Acetates ; Copper Sulfate ; Cyclopentanes ; Farms ; Oxylipins ; Plant Leaves ; Stilbenes ; Terpenes ; Volatile Organic Compounds
Chemical Substances	Acetates ; Cyclopentanes ; Oxylipins ; Stilbenes ; Terpenes ; Volatile Organic Compounds ; methyl jasmonate (900N171A0F) ; Copper Sulfate (LRX7AJ16DT)
Language	English
Publishing date	2022-09-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27186028
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Article: Phenolic content, antioxidant and anti-inflammatory activities of some Algerian olive stone extracts obtained by conventional solvent and microwave-assisted extractions under optimized conditions

Djemaa-Landri, Kenza / Hamri-Zeghichi, Sabrina / Belkhiri-Beder, Wassila / Krisa, Stéphanie / Cluzet, Stéphanie / Richard, Tristan / Valls, Josep / Kadri, Nabil / Madani, Khodir

Journal of food measurement & characterization. 2021 Oct., v. 15, no. 5

2021

Abstract: The recovery of food waste is a stimulating opportunity for and competitive development of agroindustries. In this context, the objective of this study was to report the antioxidant potential and phenolic compounds content of olive stones (OS) of six ... ...

Abstract	The recovery of food waste is a stimulating opportunity for and competitive development of agroindustries. In this context, the objective of this study was to report the antioxidant potential and phenolic compounds content of olive stones (OS) of six Algerian cultivars. Two extraction methods were used: microwave assisted extraction (MAE) and conventional solvent extraction (CSE) in order to improve the recovery of total phenolic content (TPC) and the maximization of the two extraction processes by the Box-Behnken design (BBD) from olive stone of Olea Europaea L. cv Ayemele. The optimal processing parameters obtained were (i) for MAE: 22% (v/v) ethanol, 40 s, 500 W, and ratio 65:1 (mL/g) and (ii) for CSE: 33% (v/v) ethanol, 24 min, 71 °C, and ratio 60:1 (mL/g). Phenolic compounds were then extracted from olive stones of six Algerian cultivars with MAE best extraction conditions, as it was the best strategy in term of TPC recovery. The phenolic content was different according to the olive variety. The stones of Chemlale were the richest in TPC (7.23 mg GAE/g) followed by Azeboudj (6.33 mg GAE/g), Atefah (6.04 mg GAE/g), Agraraz (5.63 mg GAE/g), Azeradj (5.35 mg GAE/g) and Ayemel (5.14 mg GAE/g). A similar profile was observed with total flavonoids content and antioxidant activities. The anti-inflammatory effects of the optimized extracts by MAE were assessed regarding their ability to prevent lipopolysaccharide-induced of reactive oxygen species and nitric oxide production in a macrophage cell line (RAW 264.7). Olive stones that are issued from the production of olive oil, and table olives can therefore be considered as renewable by-products thanks to their compounds with high added value. These bioactive compounds can target the food natural additives markets, pharmaceutical and cosmetic industries, all of which are currently very receptive such natural products.
Keywords	Olea europaea ; agricultural industry ; antioxidant activity ; cell lines ; cultivars ; experimental design ; flavonoids ; food waste ; macrophages ; measurement ; microwave treatment ; nitric oxide ; olive oil ; olives ; reactive oxygen species ; solvents
Language	English
Dates of publication	2021-10
Size	p. 4166-4180.
Publishing place	Springer US
Document type	Article
ZDB-ID	2754612-3
ISSN	2193-4134 ; 2193-4126
ISSN (online)	2193-4134
ISSN	2193-4126
DOI	10.1007/s11694-021-00992-w
Database	NAL-Catalogue (AGRICOLA)

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Article: Volatile profiles of 47 monovarietal cloudy apple juices from commercial, old, red-fleshed and scab-resistant apple cultivars

Chitarrini, Giulia / Lazazzara, Valentina / Lubes, Giuseppe / Agnolet, Sara / Valls, Josep / von Lutz, Hildegard / Brunner, Karin / Lozano, Lidia / Guerra, Walter / Ciesa, Flavio / Robatscher, Peter / Oberhuber, Michael

European food research & technology. 2021 Nov., v. 247, no. 11

2021

Abstract: This study provides cultivar-specific metabolic fingerprints of volatile organic compounds in the headspace of cloudy apple juices. Cloudy juices from 47 “true to type” apple cultivars grown under identical agricultural and climatic conditions in South ... ...

Abstract	This study provides cultivar-specific metabolic fingerprints of volatile organic compounds in the headspace of cloudy apple juices. Cloudy juices from 47 “true to type” apple cultivars grown under identical agricultural and climatic conditions in South Tyrol, Italy, have been analysed through headspace solid-phase microextraction gas chromatography coupled with high-resolution time-of-flight mass spectrometry (HS–SPME/GC–HRTOF–MS), yielding cultivar-specific volatile organic compound (VOC) profiles. Apple cultivars included 10 old cultivars with local relevance, 18 commercial cultivars, dominating the current international market, 10 scab-resistant and 9 red-fleshed ones. The 28 most abundant VOCs were found in the whole dataset, indicating a remarkable chemodiversity within juices. The main discriminant metabolites were butyl acetate; pentyl acetate; 2-methylbutyl acetate; pentan-1-ol; hexan-1-ol; n-hexanal and (E)-2-hexenal. A principal component analysis (PCA) and a hierarchical cluster analysis (HCA) have not identified a clear separation of the analysed cultivar classes. Nevertheless, a series of scab-resistant and red-fleshed cultivars with VOC profiles similar to established commercial cultivars were identified; these data could help substituting current cultivars with more sustainable ones. On a more general basis, our study supports a knowledge-driven selection of apple cultivars for specific consumer preferences and helps appreciating the biodiversity of apples across old local and more recent (scab-resistant and red-fleshed) cultivars.
Keywords	acetates ; apples ; biodiversity ; cluster analysis ; cultivars ; data collection ; food research ; gas chromatography ; headspace analysis ; mass spectrometry ; metabolites ; principal component analysis ; solid phase microextraction ; volatile organic compounds ; world markets ; Italy
Language	English
Dates of publication	2021-11
Size	p. 2739-2749.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1359456-4
ISSN	1431-4630 ; 1438-2377
ISSN	1431-4630 ; 1438-2377
DOI	10.1007/s00217-021-03826-7
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Circulating Human Metabolites Resulting from TOTUM-070 Absorption (a Plant-Based, Polyphenol-Rich Ingredient) Improve Lipid Metabolism in Human Hepatocytes: Lessons from an Original Ex Vivo Clinical Trial.

Wauquier, Fabien / Boutin-Wittrant, Line / Krisa, Stéphanie / Valls, Josep / Langhi, Cedric / Otero, Yolanda F / Sirvent, Pascal / Peltier, Sébastien / Bargetto, Maxime / Cazaubiel, Murielle / Sapone, Véronique / Bouchard-Mercier, Annie / Roux, Véronique / Macian, Nicolas / Pickering, Gisèle / Wittrant, Yohann

Nutrients

2023 Volume 15, Issue 8

Abstract: TOTUM-070 is a patented polyphenol-rich blend of five different plant extracts showing separately a latent effect on lipid metabolism and potential synergistic properties. In this study, we investigated the health benefit of such a formula. Using a ... ...

Abstract	TOTUM-070 is a patented polyphenol-rich blend of five different plant extracts showing separately a latent effect on lipid metabolism and potential synergistic properties. In this study, we investigated the health benefit of such a formula. Using a preclinical model of high fat diet, TOTUM-070 (3 g/kg of body weight) limited the HFD-induced hyperlipemia with a reduction in triglyceride (-32% after 6 weeks; -20.3% after 12 weeks) and non-HDL cholesterol levels (-21% after 6 weeks; -38.4% after 12 weeks). To further investigate such a benefit and its underlying mechanisms in humans, we designed an ex vivo clinical approach to collect the circulating bioactives resulting from TOTUM-070 ingestion and to determine their biological activities on human hepatocytes. Human serum was obtained from healthy subjects before and after intake of TOTUM-070 (4995 mg). The presence of circulating metabolites was assessed by UPLC-MS/MS. Serum containing metabolites was further incubated with hepatocytes cultured in a lipotoxic environment (palmitate, 250 µM). RNA sequencing analyses show that lipid metabolism was one of the most impacted processes. Using histologic, proteomic, and enzymatic assays, the effects of human TOTUM-070 bioactives on hepatocyte metabolism were characterized by (1) the inhibition of lipid storage, including both (2) triglycerides (-41%,
MeSH term(s)	Humans ; Lipid Metabolism ; Polyphenols/pharmacology ; Polyphenols/metabolism ; Chromatography, Liquid ; Proteomics ; Tandem Mass Spectrometry ; Hepatocytes ; Cholesterol ; Triglycerides ; Diet, High-Fat ; Liver/metabolism
Chemical Substances	Polyphenols ; Cholesterol (97C5T2UQ7J) ; Triglycerides
Language	English
Publishing date	2023-04-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu15081903
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Verification: model-free phasing with enhanced predicted models in ARCIMBOLDO_SHREDDER.

Medina, Ana / Jiménez, Elisabet / Caballero, Iracema / Castellví, Albert / Triviño Valls, Josep / Alcorlo, Martin / Molina, Rafael / Hermoso, Juan A / Sammito, Massimo D / Borges, Rafael / Usón, Isabel

Acta crystallographica. Section D, Structural biology

2022 Volume 78, Issue Pt 11, Page(s) 1283–1293

Abstract: Structure predictions have matched the accuracy of experimental structures from close homologues, providing suitable models for molecular replacement phasing. Even in predictions that present large differences due to the relative movement of domains or ... ...

Abstract	Structure predictions have matched the accuracy of experimental structures from close homologues, providing suitable models for molecular replacement phasing. Even in predictions that present large differences due to the relative movement of domains or poorly predicted areas, very accurate regions tend to be present. These are suitable for successful fragment-based phasing as implemented in ARCIMBOLDO. The particularities of predicted models are inherently addressed in the new predicted_model mode, rendering preliminary treatment superfluous but also harmless. B-value conversion from predicted LDDT or error estimates, the removal of unstructured polypeptide, hierarchical decomposition of structural units from domains to local folds and systematically probing the model against the experimental data will ensure the optimal use of the model in phasing. Concomitantly, the exhaustive use of models and stereochemistry in phasing, refinement and validation raises the concern of crystallographic model bias and the need to critically establish the information contributed by the experiment. Therefore, in its predicted_model mode ARCIMBOLDO_SHREDDER will first determine whether the input model already constitutes a solution or provides a straightforward solution with Phaser. If not, extracted fragments will be located. If the landscape of solutions reveals numerous, clearly discriminated and consistent probes or if the input model already constitutes a solution, model-free verification will be activated. Expansions with SHELXE will omit the partial solution seeding phases and all traces outside their respective masks will be combined in ALIXE, as far as consistent. This procedure completely eliminates the molecular replacement search model in favour of the inferences derived from this model. In the case of fragments, an incorrect starting hypothesis impedes expansion. The predicted_model mode has been tested in different scenarios.
MeSH term(s)	Crystallography, X-Ray ; Models, Molecular ; Peptides
Chemical Substances	Peptides
Language	English
Publishing date	2022-10-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2968623-4
ISSN	2059-7983 ; 0907-4449
ISSN (online)	2059-7983
ISSN	0907-4449
DOI	10.1107/S2059798322009706
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Article: Attractiveness analysis of European tourist cities

Valls, Josep-Francesc / Sureda, Joan / Valls-Tuñon, Gerard

Journal of travel and tourism marketing Vol. 31, No. 1/2 , p. 178-194

2014 Volume 31, Issue 1, Page(s) 178–194

Author's details	Josep-Francesc Valls; Joan Sureda; Gerard Valls-Tuñon
Keywords	City competitiveness ; city marketing ; city break ; urban tourism ; European lifestyles
Language	English
Size	graph. Darst.
Publisher	Haworth Press
Publishing place	Binghamton, NY
Document type	Article
ZDB-ID	1408990-7
ISSN	1054-8408
Database	ECONomics Information System

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Article: Phenolic content and antioxidant activities of Vitis vinifera L. leaf extracts obtained by conventional solvent and microwave-assisted extractions

Djemaa-Landri, Kenza / Hamri-Zeghichi, Sabrina / Valls, Josep / Cluzet, Stéphanie / Tristan, Richard / Boulahbal, Nawel / Kadri, Nabil / Madani, Khodir

Journal of food measurement & characterization. 2020 Dec., v. 14, no. 6

2020

Abstract: Grapevine leaves are used in the cuisines in the countries surrounding the Mediterranean and in folk medicine. They contain a huge amount of phenolic constituents. To optimize the extraction of bioactive compounds present in grapevine leaves, two methods ...

Abstract	Grapevine leaves are used in the cuisines in the countries surrounding the Mediterranean and in folk medicine. They contain a huge amount of phenolic constituents. To optimize the extraction of bioactive compounds present in grapevine leaves, two methods of extraction were compared: conventional solvent extraction (CSE) and microwave-assisted extraction (MAE). The optimal extraction conditions in term of total phenolic content (TPC) were determined using a Box–Behnken design (BBD) from leaves of Vitis vinifera L. cv. Le tizourine Bou Afraraet. Optimal extraction conditions for CSE was 29% concentration of ethanol (v/v), 30.96 min extraction time, and 72:1 liquid-to-solid ratio (mL:g), at 37.5 °C. For MAE it was 34% concentration of ethanol(v/v), 474 W microwave power, 47 s irradiation time and 40:1 liquid-to-solid ratio (mL:g). Both extracts obtained by optimized MAE and CSE processes were compared in terms of total flavonoid content (TFC) and antioxidant activities (DPPH, ORAC, PFRAP and ICA). The MAE extract exhibited highest phenolic content and antioxidant activities. Moreover, phenolic compounds from leaves of Vitis vinifera L. cv. Ahmar-Bou-Amar were extracted following MAE optimal conditions and compared to Le tizourine Bou Afraraet extract. In addition to TPC and antioxidant activities, phenolic constituents were determined using HPLC–DAD-IT-MS and UPLC-QqQ-MS/MS. A total of sixteen phenolic compounds were identified and quantified including two phenolic acids, seven flavonols, three flavanols and four stilbenes. The obtained data revealed that the leaves of two native Algerian grapevines are rich in bioactive constituents, and can constitute by-products with added-value for food supplements.
Keywords	Vitis vinifera ; antioxidants ; ethanol ; experimental design ; flavanols ; flavonols ; irradiation ; leaves ; measurement ; microwave treatment ; solvents ; stilbenes ; traditional medicine
Language	English
Dates of publication	2020-12
Size	p. 3551-3564.
Publishing place	Springer US
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2754612-3
ISSN	2193-4134 ; 2193-4126
ISSN (online)	2193-4134
ISSN	2193-4126
DOI	10.1007/s11694-020-00596-w
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine.

Lemaitre-Guillier, Christelle / Dufresne, Christelle / Chartier, Agnès / Cluzet, Stéphanie / Valls, Josep / Jacquens, Lucile / Douillet, Antonin / Aveline, Nicolas / Adrian, Marielle / Daire, Xavier

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 14

Abstract: Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine ...

Abstract	Grapevine is susceptible to fungal diseases generally controlled by numerous chemical fungicides. Elicitors of plant defence are a way of reducing the use of these chemicals, but still provide inconsistent efficiency. Easy-to-analyse markers of grapevine responses to elicitors are needed to determine the best conditions for their efficiency and position them in protection strategies. We previously reported that the elicitor sulphated laminarin induced the emission of volatile organic compounds (VOCs) by grapevine leaves. The present study was conducted to characterise and compare VOC emissions in response to other elicitors. Bastid
MeSH term(s)	Fungicides, Industrial/analysis ; Gas Chromatography-Mass Spectrometry ; Plant Diseases/microbiology ; Plant Leaves/chemistry ; Plant Leaves/microbiology ; Sesquiterpenes/analysis ; Vitis/chemistry ; Vitis/microbiology ; Volatile Organic Compounds/analysis
Chemical Substances	Fungicides, Industrial ; Sesquiterpenes ; Volatile Organic Compounds ; alpha-farnesene (502-61-4)
Language	English
Publishing date	2021-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26144258
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Article ; Online: Wood and roots of major grapevine cultivars and rootstocks: A comparative analysis of stilbenes by UHPLC-DAD-MS/MS and NMR.

Gabaston, Julien / Leborgne, Cécile / Waffo-Teguo, Pierre / Valls, Josep / Palos Pinto, Antonio / Richard, Tristan / Cluzet, Stéphanie / Mérillon, Jean-Michel

Phytochemical analysis : PCA

2019 Volume 30, Issue 3, Page(s) 320–331

Abstract: Introduction: Grapevine wood and roots are by-products obtained during vineyard management. This plentiful biomass is known to be rich in stilbenes and can be used as a source of high-value compounds as well as active natural extracts. However, the ... ...

Abstract	Introduction: Grapevine wood and roots are by-products obtained during vineyard management. This plentiful biomass is known to be rich in stilbenes and can be used as a source of high-value compounds as well as active natural extracts. However, the stilbenes in grapevine wood and roots from different cultivars and rootstocks remain to be characterized. Objective: The present study investigated the stilbene content of eight major Vitis vinifera cultivars and six different rootstocks. In addition, the distribution of stilbenes was established for each of seven parts into which the plants were sub-divided. Methodology: For stilbene characterization and quantification purposes, an ultra-high performance liquid chromatography-diode array detector-mass spectrometry (UHPLC-DAD-MS/MS) analysis of different samples was carried out. Moreover, structural data of stilbenes was unambiguously studied by nuclear magnetic resonance (NMR) spectra. Results: Whatever the cultivar/rootstock combination, stilbenes were found to be oligomerized from the aerial part to the root system. Furthermore, stilbene content varied widely depending on the cultivars and rootstocks. For instance, the cultivars Merlot, Tannat and Gamay noir were the richest in stilbenes while the rootstocks Gravesac, Fercal and 3390C contained the highest amounts. Conclusion: These findings provide insight into the knowledge that major grapevine cultivars and rootstocks can be used as a potential source of complex stilbenes.
MeSH term(s)	Chromatography, High Pressure Liquid/methods ; Plant Extracts/analysis ; Plant Extracts/chemistry ; Plant Roots/chemistry ; Polymerization ; Proton Magnetic Resonance Spectroscopy/methods ; Stilbenes/analysis ; Tandem Mass Spectrometry/methods ; Vitis/chemistry ; Wood
Chemical Substances	Plant Extracts ; Stilbenes
Language	English
Publishing date	2019-01-15
Publishing country	England
Document type	Comparative Study ; Journal Article
ZDB-ID	1073576-8
ISSN	1099-1565 ; 0958-0344
ISSN (online)	1099-1565
ISSN	0958-0344
DOI	10.1002/pca.2815
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