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Article ; Online: Maternal plasma markers in intrauterine growth restriction and small for gestational age complicated pregnancy: the role of sFlt-1/PlGF.

Libretti, Alessandro / Valsecchi, Luca / Zerbini, Gianpaolo / Remorgida, Valentino / Candiani, Massimo

2023 Volume 75, Issue 6, Page(s) 588–589

MeSH term(s)	Pregnancy ; Infant, Newborn ; Female ; Humans ; Fetal Growth Retardation/diagnosis ; Gestational Age ; Placenta Growth Factor ; Infant, Small for Gestational Age ; Vascular Endothelial Growth Factor Receptor-1
Chemical Substances	Placenta Growth Factor (144589-93-5) ; Vascular Endothelial Growth Factor Receptor-1 (EC 2.7.10.1)
Language	English
Publishing date	2023-08-04
Publishing country	Italy
Document type	Journal Article ; Comment
ZDB-ID	3062815-5
ISSN	2724-6450
ISSN (online)	2724-6450
DOI	10.23736/S2724-606X.23.05384-8
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Article: Methylmercury determination in freshwater biota and sediments: Static headspace GC-MS compared to direct mercury analyzer.

Valsecchi, Lucia / Roscioli, Claudio / Schiavon, Alfredo / Marziali, Laura

MethodsX

2021 Volume 8, Page(s) 101581

Abstract: We developed and compared two analytical methods for determination of MeHg in freshwater biota and sediments, by: I) simplified static headspace GC-MS using internal standard (IS) isotope dilution quantification, after microwave acid digestion and ... ...

Abstract	We developed and compared two analytical methods for determination of MeHg in freshwater biota and sediments, by: I) simplified static headspace GC-MS using internal standard (IS) isotope dilution quantification, after microwave acid digestion and aqueous phase NaBEt
Language	English
Publishing date	2021-11-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2021.101581
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Article ; Online: Mercury Bioaccumulation in Benthic Invertebrates: From Riverine Sediments to Higher Trophic Levels.

Marziali, Laura / Roscioli, Claudio / Valsecchi, Lucia

Toxics

2021 Volume 9, Issue 9

Abstract: Riverine sediments are important sites of mercury methylation and benthic invertebrates may be indicators of Hg exposure to higher organisms. From 2014 to 2018, sediments and invertebrates were collected along a mercury gradient in the Toce River ( ... ...

Abstract	Riverine sediments are important sites of mercury methylation and benthic invertebrates may be indicators of Hg exposure to higher organisms. From 2014 to 2018, sediments and invertebrates were collected along a mercury gradient in the Toce River (Northern Italy) and analyzed for THg and MeHg. Concentrations in invertebrates, separated according to taxon and to Functional Feeding Group, ranged from 20 to 253 µg kg
Language	English
Publishing date	2021-08-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2733883-6
ISSN	2305-6304 ; 2305-6304
ISSN (online)	2305-6304
ISSN	2305-6304
DOI	10.3390/toxics9090197
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Article ; Online: Methylmercury determination in freshwater biota and sediments: Static headspace GC-MS compared to direct mercury analyzer

Valsecchi, Lucia / Roscioli, Claudio / Schiavon, Alfredo / Marziali, Laura

MethodsX. 2021, v. 8 p.101581-

2021

Abstract	We developed and compared two analytical methods for determination of MeHg in freshwater biota and sediments, by: I) simplified static headspace GC-MS using internal standard (IS) isotope dilution quantification, after microwave acid digestion and aqueous phase NaBEt₄ ethylation; II) Automated Mercury Analyzer, after double toluene extraction followed by back-extraction with L-cystein. The performance was evaluated by analysis of certified reference materials. For biota, mean recovery was 100 ± 2% and relative standard deviation (RSD) ≤ 6.8% for method I, and mean recovery was 98 ± 7% and RSD ≤13% for method II. For sediments, recovery of 94.5% and RSD of 8.8% were obtained with method I, and recovery of 90.3% and RSD of 9.4% with method II. Limits of detection (LOD) were 0.7 µg kg⁻¹ and 6 µg kg⁻¹, respectively. Both techniques were tested for MeHg analysis in freshwater invertebrates, fish and sediments, covering a large range of MeHg values (1.9–670 µg kg⁻¹ d.w.). • Both protocols proved to be suitable for MeHg analysis in complex environmental matrices, even if, for method II, interferences in the extraction phase and limited sensitivity may hinder sediment analysis. • Passing-Bablock regression revealed a slight disproportion between methods, with line slope = 1.058 (95% CI ranging from 1.001 to 1.090).
Keywords	ethylation ; fish ; freshwater ; headspace analysis ; isotope dilution technique ; mercury ; methylmercury compounds ; sediments ; standard deviation ; toluene ; wet digestion method ; Methylmercury in freshwater biota and sediments by GC-MS or automatic mercury analyzer ; Organometallic compounds ; Methylmercury determination ; Benthic invertebrates
Language	English
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2021.101581
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Article ; Online: Vertical profiles of trace elements in a sediment core from the Lambro River (northern Italy): Historical trends and pollutant transport to the Adriatic Sea.

Marziali, Laura / Valsecchi, Lucia / Schiavon, Alfredo / Mastroianni, Domenico / Viganò, Luigi

The Science of the total environment

2021 Volume 782, Page(s) 146766

Abstract: River sediments generally act as a sink for trace elements but, when resuspended, they contribute to long-term downstream transport of contamination, which may finally reach the marine environment. This study analyzed these processes in a complex aquatic ...

Abstract	River sediments generally act as a sink for trace elements but, when resuspended, they contribute to long-term downstream transport of contamination, which may finally reach the marine environment. This study analyzed these processes in a complex aquatic system that includes a contaminated tributary, the Lambro River (Northern Italy) and its recipient and main Italian watercourse, the Po River, with the prodelta in the Adriatic Sea. The study was conducted from a historical perspective which, covering the last 50 years, examined the main driving events such as the inputs of contaminants, the construction of WWTPs and the evolution of environmental legislation. The time trend of trace element contamination was analyzed in a sediment core collected in the Lambro River and dated 1962-2011. The highest enrichments were found for Hg, Zn, Cu, Pb and Cd, which showed similar trends, with EF maxima in the '60s-'90s (172, 56, 40, 28 and 21, respectively), following industrial and urban development, and a general decreasing pattern after the late '90s. Only in the 2000s the ecological risk associated with metal contamination showed mean PEC Quotients stably below 1. The results of a literature survey on sedimentary trace elements in the Po River and the prodelta for the last 50 years were then compared to the Lambro sediment core. A significant contribution to Cu, Zn, Pb, Hg and Cd contamination was proved to derive from Lambro sediment transport. In the prodelta, increasing Ni and Cr concentrations were also evidenced, likely as a result of enhanced soil erosion in the Po basin. This study highlights the key role of WWTPs, of lower-impact industrial processes and of environmental legislation in reducing contaminant inputs. It also emphasizes the active contribution of riverine sediment-bound contamination to long-distance marine sediment quality.
Language	English
Publishing date	2021-03-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2021.146766
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Article ; Online: Phosphate adsorption by riverborne clay sediments in a southern-Italy Mediterranean reservoir: Insights from a "natural geo-engineering" experiment.

Copetti, Diego / Valsecchi, Lucia / Tartari, Gianni / Mingazzini, Marina / Palumbo, Maria Teresa

The Science of the total environment

2022 Volume 856, Issue Pt 2, Page(s) 159225

Abstract: This paper reports data from a southern-Italy reservoir (Lake Occhito) characterized by a strong riverborne sediment transport. Main hydrochemical, trophic, and nutrient variables were measured (over a twelve-month period) in both lake and tributaries. ... ...

Abstract	This paper reports data from a southern-Italy reservoir (Lake Occhito) characterized by a strong riverborne sediment transport. Main hydrochemical, trophic, and nutrient variables were measured (over a twelve-month period) in both lake and tributaries. Lacustrine sediments were subjected to mineralogical characterization and to phosphorus fractioning, while a 6-day long batch experiment was carried out to evaluate the lake sediment orthophosphate adsorption capacity. A set of algal growth potential tests was also undertaken on the lake and its tributaries. Results highlight the presence of a strong gradient in nutrient availability among the inflows. Most of the nutrient loads were from the main tributary (20.3 t P a
MeSH term(s)	Clay ; Geologic Sediments/chemistry ; Phosphates ; Adsorption ; Ecosystem ; Lakes/chemistry ; Phosphorus/chemistry ; Water Pollutants, Chemical/analysis ; Eutrophication
Chemical Substances	Clay (T1FAD4SS2M) ; Phosphates ; Phosphorus (27YLU75U4W) ; Water Pollutants, Chemical
Language	English
Publishing date	2022-10-05
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2022.159225
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Article ; Online: Effects of flexible sacrum position at birth on maternal and neonatal outcomes: A retrospective cohort study.

Caglioni, Martina / Cantatore, Francesco / Valsecchi, Luca / Miglioli, Cesare / Dumont, Roxane / Rinaldi, Stefania / Candiani, Massimo / Salvatore, Stefano

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics

2023 Volume 163, Issue 3, Page(s) 911–919

Abstract: Objective: To examine the differences in both maternal and neonatal outcomes between flexible and non-flexible sacrum positions at birth.: Methods: A descriptive, cross-sectional, retrospective study was carried out on a sample of low-risk pregnant ... ...

Abstract	Objective: To examine the differences in both maternal and neonatal outcomes between flexible and non-flexible sacrum positions at birth. Methods: A descriptive, cross-sectional, retrospective study was carried out on a sample of low-risk pregnant women. Univariate and multivariate logistic regressions and multivariate linear regressions were conducted to estimate the association between our discrete or continuous variables of interest. Maternal outcomes were perineal tear, maternal blood loss, second stage length; neonatal outcomes were Apgar scores and neonatal asphyxia. Results were adjusted for maternal age, neonatal birth weight, and epidural analgesia. Results: We considered for final analysis 2198 women. In primiparous women, women giving birth in the all-fours position were significantly more likely to have an intact perineum (P = 0.011) and a shorter length of the second stage of labor (P = 0.022). Maternal age (P = 0.005) and neonatal weight (P = 0.013) significantly increased perineal tearing; maternal age (P = 0.004) and neonatal birth weight (P < 0.001) were significantly associated with a higher amount of blood loss. Maternal age (P = 0.002) and neonatal weight (P < 0.001) significantly increased the length of the second stage of labor. For multiparous women, the side-lying position was significantly correlated with an intact perineum (P = 0.031); maternal age and intact perineum were statistically inversely associated. Epidural analgesia significantly increased the length of the second stage of labor in both nulliparous (P < 0.001) and pluriparous women (P < 0.001). No significant differences were found in neonatal outcomes. Conclusion: Women with a low-risk labor should be free to choose their birth position as flexible sacrum positions are shown to increase maternal well-being and do not affect neonatal health.
MeSH term(s)	Infant, Newborn ; Pregnancy ; Female ; Humans ; Retrospective Studies ; Sacrum ; Birth Weight ; Cross-Sectional Studies ; Labor, Obstetric
Language	English
Publishing date	2023-06-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	80149-5
ISSN	1879-3479 ; 0020-7292
ISSN (online)	1879-3479
ISSN	0020-7292
DOI	10.1002/ijgo.14897
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Article ; Online: Direct measurement of nutrient concentrations in freshwaters with a miniaturized analytical probe: evaluation and validation.

Copetti, D / Valsecchi, L / Capodaglio, A G / Tartari, G

Environmental monitoring and assessment

2017 Volume 189, Issue 4, Page(s) 144

Abstract: This work deals with the evaluation of the aqueous concentrations of dissolved reactive phosphorus (DRP), total phosphorus (TP), and ammonium nitrogen (N- ... ...

Abstract	This work deals with the evaluation of the aqueous concentrations of dissolved reactive phosphorus (DRP), total phosphorus (TP), and ammonium nitrogen (N-NH
MeSH term(s)	Environmental Monitoring ; Fresh Water/analysis ; Fresh Water/chemistry ; Nitrogen/analysis ; Phosphorus/analysis ; Rain ; Rivers ; Sewage/analysis ; Water/analysis ; Water Pollutants, Chemical/analysis
Chemical Substances	Sewage ; Water Pollutants, Chemical ; Water (059QF0KO0R) ; Phosphorus (27YLU75U4W) ; Nitrogen (N762921K75)
Language	English
Publishing date	2017-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/s10661-017-5847-0
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Article ; Online: High dose antithrombin supplementation in early preeclampsia: A randomized, double blind, placebo-controlled study.

D'Angelo, Armando / Valsecchi, Luca

Thrombosis research

2016 Volume 140, Page(s) 7–13

Abstract: Introduction: Antithrombin levels are often reduced in preeclampsia and infusion of antithrombin concentrates has been reported to prolong gestation in severe preeclampsia. We aimed to evaluate efficacy and safety of high-dose antithrombin (ATIII) ... ...

Abstract	Introduction: Antithrombin levels are often reduced in preeclampsia and infusion of antithrombin concentrates has been reported to prolong gestation in severe preeclampsia. We aimed to evaluate efficacy and safety of high-dose antithrombin (ATIII) supplementation in patients with single pregnancies and preeclampsia occurring before 30 weeks of gestation. Materials and methods: In November 2004 a double-blind, placebo-controlled trial (code KB033) was started in 13 Italian centers. The planned sample size was of 240 patients (intention-to-treat, ITT population) to detect a 30% relative risk reduction of the primary endpoint, composite perinatal morbidity. Eligible patients were randomized to high dose AT (3000 IU/daily, ATIII Kedrion S.p.A., Italy), or placebo (1% glycine) for 7 days or less until delivery, whichever came first. The per-protocol (PP) population was restricted to patients receiving at least two days of treatment. Results: The study was terminated by the sponsor in October 2007 after the enrolment of 38 evaluable patients - 20 randomized to high dose AT and 18 to placebo, 27 treated for 2 days or more - out of 164 screened patients. Enrolment failures were mainly represented by requirement for immediate delivery and consent refusal (91 patients). The primary endpoint occurred in 15 of 38 patients (39.5%), with a relative risk in the AT arm of 0.85 (95% CI 0.42-1.75) and 0.79 (95% CI 0.30-2.11) in the ITT and PP populations, respectively. Living neonates in the two arms had similar weight at birth, Apgar scores, and duration of hospitalization in neonatal ICU. In mothers, AT supplementation was associated with reduced blood loss at delivery and with surrogate laboratory markers (LDH, d-dimer). Conclusions: The results of this markedly underpowered trial, albeit suggestive of a potential maternal benefit, cannot support high-dose AT supplementation to improve fetal/neonatal outcomes in early preeclampsia.
MeSH term(s)	Adult ; Antithrombin III/administration & dosage ; Antithrombin III/therapeutic use ; Antithrombins/administration & dosage ; Antithrombins/therapeutic use ; Double-Blind Method ; Female ; Fibrin Fibrinogen Degradation Products/analysis ; Humans ; Infant, Newborn ; Placebo Effect ; Pre-Eclampsia/blood ; Pre-Eclampsia/drug therapy ; Pregnancy ; Treatment Outcome
Chemical Substances	Antithrombins ; Fibrin Fibrinogen Degradation Products ; fibrin fragment D ; Antithrombin III (9000-94-6)
Language	English
Publishing date	2016-04
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	121852-9
ISSN	1879-2472 ; 0049-3848
ISSN (online)	1879-2472
ISSN	0049-3848
DOI	10.1016/j.thromres.2016.01.024
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Article ; Online: Toxicity risk assessment of mercury, DDT and arsenic legacy pollution in sediments: A triad approach under low concentration conditions.

Marziali, L / Rosignoli, F / Drago, A / Pascariello, S / Valsecchi, L / Rossaro, B / Guzzella, L

The Science of the total environment

2017 Volume 593-594, Page(s) 809–821

Abstract: The determination of sediment toxicity is challenging due to site-specific factors affecting pollutants distribution and bioavailability, especially when contamination levels are close to expected non-effect concentrations. Different lines of evidence ... ...

Abstract	The determination of sediment toxicity is challenging due to site-specific factors affecting pollutants distribution and bioavailability, especially when contamination levels are close to expected non-effect concentrations. Different lines of evidence and sensitive tools are necessary for a proper toxicity risk assessment. We examined the case study of the Toce River (Northern Italy), where past industrial activities determined Hg, DDT and As enrichment in sediments. A triad approach comprising chemical, ecotoxicological and ecological analyses (benthic invertebrates) was carried out for risk assessment of residual contamination in river sediments. A "blank" site upstream from the industrial site was selected to compare the other sites downstream. Sediment, water and benthic invertebrate samplings were carried out following standard protocols. Results emphasized that despite the emissions of the industrial site ceased about 20years ago, sediments in the downstream section of the river remain contaminated by Hg, DDT and As with concentrations exceeding Threshold Effect Concentrations. A chronic whole-sediment test with Chironomus riparius showed decreased development rate and a lower number of eggs per mass in the contaminated sediments. Benthic community was analyzed with the calculation of integrated (STAR_ICMi) and stressor-specific metrics (SPEAR
MeSH term(s)	Animals ; Arsenic/toxicity ; Chironomidae ; DDT/toxicity ; Ecotoxicology ; Environmental Monitoring ; Geologic Sediments/chemistry ; Invertebrates ; Italy ; Mercury/toxicity ; Risk Assessment ; Rivers/chemistry ; Water Pollutants, Chemical/toxicity
Chemical Substances	Water Pollutants, Chemical ; DDT (CIW5S16655) ; Mercury (FXS1BY2PGL) ; Arsenic (N712M78A8G)
Language	English
Publishing date	2017-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2017.03.219
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