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Article: A Persistent Additional Fraction on Capillary Zone Electrophoresis with Negative Immunofixation Electrophoresis: Paraproteinemia or Pseudoparaproteinemia?

Eeckhout, Katrien / Van Cotthem, Karin / Van Hoof, Viviane / Guerti, Khadija

The journal of applied laboratory medicine

2020 Volume 6, Issue 3, Page(s) 790–794

MeSH term(s)	Electrophoresis, Capillary ; Humans ; Immunoelectrophoresis ; Paraproteinemias/diagnosis
Language	English
Publishing date	2020-09-18
Publishing country	England
Document type	Journal Article
ISSN	2576-9456
ISSN	2576-9456
DOI	10.1093/jalm/jfaa085
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Article ; Online: Skeletal muscle biomarkers in heart disease.

Van Hoof, Viviane

Biomarkers in medicine

2012 Volume 6, Issue 1, Page(s) 5–8

MeSH term(s)	Biomarkers/metabolism ; Cell Membrane/metabolism ; Clinical Chemistry Tests/methods ; Clinical Chemistry Tests/standards ; Heart Diseases/metabolism ; Heart Diseases/pathology ; Humans ; Hypoxia/metabolism ; Muscle, Skeletal/metabolism ; Necrosis ; Reference Values ; Time Factors ; Troponin/metabolism
Chemical Substances	Biomarkers ; Troponin
Language	English
Publishing date	2012-02
Publishing country	England
Document type	Editorial
ZDB-ID	2481014-9
ISSN	1752-0371 ; 1752-0363
ISSN (online)	1752-0371
ISSN	1752-0363
DOI	10.2217/bmm.11.103
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Article ; Online: Failure Mode and Effects Analysis (FMEA) at the preanalytical phase for POCT blood gas analysis: proposal for a shared proactive risk analysis model.

Van Hoof, Viviane / Bench, Suzanne / Soto, Antonio Buño / Luppa, Peter P / Malpass, Anthony / Schilling, Ulf Martin / Rooney, Kevin D / Stretton, Adam / Tintu, Andrei N

Clinical chemistry and laboratory medicine

2022 Volume 60, Issue 8, Page(s) 1186–1201

Abstract: Objectives: Proposal of a risk analysis model to diminish negative impact on patient care by preanalytical errors in blood gas analysis (BGA).: Methods: Here we designed a Failure Mode and Effects Analysis (FMEA) risk assessment template for BGA, ... ...

Abstract	Objectives: Proposal of a risk analysis model to diminish negative impact on patient care by preanalytical errors in blood gas analysis (BGA). Methods: Here we designed a Failure Mode and Effects Analysis (FMEA) risk assessment template for BGA, based on literature references and expertise of an international team of laboratory and clinical health care professionals. Results: The FMEA identifies pre-analytical process steps, errors that may occur whilst performing BGA (potential failure mode), possible consequences (potential failure effect) and preventive/corrective actions (current controls). Probability of failure occurrence (OCC), severity of failure (SEV) and probability of failure detection (DET) are scored per potential failure mode. OCC and DET depend on test setting and patient population e.g., they differ in primary community health centres as compared to secondary community hospitals and third line university or specialized hospitals. OCC and DET also differ between stand-alone and networked instruments, manual and automated patient identification, and whether results are automatically transmitted to the patient's electronic health record. The risk priority number (RPN = SEV × OCC × DET) can be applied to determine the sequence in which risks are addressed. RPN can be recalculated after implementing changes to decrease OCC and/or increase DET. Key performance indicators are also proposed to evaluate changes. Conclusions: This FMEA model will help health care professionals manage and minimize the risk of preanalytical errors in BGA.
MeSH term(s)	DEET ; Healthcare Failure Mode and Effect Analysis ; Humans ; Pre-Analytical Phase ; Probability ; Risk Assessment
Chemical Substances	DEET (134-62-3)
Language	English
Publishing date	2022-05-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2022-0319
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Article ; Online: Accuracy, user-friendliness and usefulness of the Cobas h232 point-of-care test for NT-proBNP in primary care.

Hex, Chiel / Smeets, Miek / Penders, Joris / Van Hoof, Viviane / Verbakel, Jan / Buntinx, Frank / Vaes, Bert

Journal of clinical pathology

2018 Volume 71, Issue 6, Page(s) 539–545

Abstract: Aims: N-terminal pro-B-type natriuretic peptide (NT-proBNP) has been shown to be useful for ruling out heart failure in primary care. In this study, we examined the accuracy of the Cobas h232 point-of-care (POC) instrument in primary care compared with ... ...

Abstract	Aims: N-terminal pro-B-type natriuretic peptide (NT-proBNP) has been shown to be useful for ruling out heart failure in primary care. In this study, we examined the accuracy of the Cobas h232 point-of-care (POC) instrument in primary care compared with an in-hospital measurement. Furthermore, we investigated the user-friendliness and usefulness of the POC device. Methods: Five general practitioner (GP) groups were asked to evaluate adult patients who were suspected of having heart failure and to test NT-proBNP with the Cobas h232. The samples were subsequently delivered to and analysed at a central hospital laboratory by the Cobas e602 using conventional transport and storage. Difference between the paired measurements was analysed using a percentage difference plot, and correlation was assessed using Passing-Bablok linear regression analysis. User-friendliness and usefulness were assessed using semistructured questionnaires. Results: Nineteen GPs studied 94 patients. Passing-Bablok analysis showed a slope of 1.05 (95% CI 1.00 to 1.11) (R Conclusions: The Cobas h232 NT-proBNP POC test proved to be an accurate, user-friendly and useful test in primary care. Nearly all participating GPs were convinced that the test could benefit clinical decision making.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Attitude of Health Personnel ; Belgium ; Biomarkers/blood ; Clinical Laboratory Techniques ; Female ; General Practitioners/psychology ; Health Knowledge, Attitudes, Practice ; Heart Failure/blood ; Heart Failure/diagnosis ; Heart Function Tests/methods ; Humans ; Male ; Middle Aged ; Natriuretic Peptide, Brain/blood ; Peptide Fragments/blood ; Pilot Projects ; Point-of-Care Testing ; Predictive Value of Tests ; Primary Health Care/methods ; Reproducibility of Results ; Surveys and Questionnaires
Chemical Substances	Biomarkers ; Peptide Fragments ; pro-brain natriuretic peptide (1-76) ; Natriuretic Peptide, Brain (114471-18-0)
Language	English
Publishing date	2018-06
Publishing country	England
Document type	Comparative Study ; Evaluation Studies ; Journal Article ; Multicenter Study
ZDB-ID	80261-x
ISSN	1472-4146 ; 0021-9746
ISSN (online)	1472-4146
ISSN	0021-9746
DOI	10.1136/jclinpath-2017-204746
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Article ; Online: Organisation and quality monitoring for point-of-care testing (POCT) in Belgium: proposal for an expansion of the legal framework for POCT into primary health care.

Van Hoof, Viviane / Barglazan, Dragos / Blairon, Laurent / Braekevelt, Bob / Debois, Regis / De Vos, Nathalie Véronique J / Gruson, Damien / Jonckheere, Jef / Lanckmans, Katrien / Moens, Marc / Peeters, Bart / Penders, Joris / Roman, Alain / Van Hoovels, Lieve / Vanstapel, Florent / Verbakel, Jan Y / Verdonck, Ann / Verstraete, Alain G

Acta clinica Belgica

2021 Volume 77, Issue 2, Page(s) 329–336

Abstract: Background: There is a trend towards decentralisation of laboratory tests by means of Point-of-Care testing (POCT). Within hospitals, Belgian law requires a POCT policy, coordinated by the clinical laboratory. There is however no legal framework for ... ...

Abstract	Background: There is a trend towards decentralisation of laboratory tests by means of Point-of-Care testing (POCT). Within hospitals, Belgian law requires a POCT policy, coordinated by the clinical laboratory. There is however no legal framework for POCT performed outside the hospital: no reimbursement, no compulsory quality monitoring and no limits nor control on the prices charged to the patient. Uncontrolled use of POCT can have negative consequences for individual and public health. Proposal: We propose that POCT outside hospitals would only be reimbursed for tests carried out within a legal framework, requiring evidence-based testing and collaboration with a clinical laboratory, because clinical laboratories have procedures for test validation and quality monitoring, are equipped for electronic data transfer, are familiar with logistical processes, can provide support when technical issues arise and can organise and certify training. Under these conditions the government investment will be offset by health benefits, e.g. fall in antibiotic consumption with POCT for CRP in primary care, quick response to SARS-CoV2-positive cases in COVID-19 triage centres. Priorities: 1° extension of the Belgian decree on certification of clinical laboratories to decentralised tests in primary care; 2° introduction of a separate reimbursement category for POCT; 3° introduction of reimbursement for a limited number of specified POCT; 4° setup of a Multidisciplinary POCT Advisory Council, the purpose of which is to draw up a model for reimbursement of POCT, to select tests eligible for reimbursement and to make proposals to the National Institute for Health and Disability Insurance (
MeSH term(s)	Belgium ; COVID-19/diagnosis ; COVID-19/epidemiology ; Humans ; Point-of-Care Systems ; Point-of-Care Testing ; Primary Health Care ; RNA, Viral ; SARS-CoV-2
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-01-06
Publishing country	England
Document type	Journal Article
ZDB-ID	390201-8
ISSN	2295-3337 ; 0001-5512 ; 1784-3286
ISSN (online)	2295-3337
ISSN	0001-5512 ; 1784-3286
DOI	10.1080/17843286.2020.1868906
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Article ; Online: Efficient voltammetric discrimination of free bilirubin from uric acid and ascorbic acid by a CVD nanographite-based microelectrode.

Taurino, Irene / Van Hoof, Viviane / Magrez, Arnaud / Forró, László / De Micheli, Giovanni / Carrara, Sandro

Talanta

2014 Volume 130, Page(s) 423–426

Abstract: We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the ... ...

Abstract	We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the bilirubin therefore reducing the concentration of the free electroactive metabolite in human fluids. In addition, the proposed device permits the discrimination of free bilirubin from two interferents, uric acid and ascorbic acid, by the separation of their oxidation peaks in voltammetry. Preliminary measurements in human serum prove that the proposed nanostructured platform can be used to detect bilirubin.
MeSH term(s)	Ascorbic Acid/analysis ; Ascorbic Acid/chemistry ; Bilirubin/analysis ; Bilirubin/chemistry ; Biosensing Techniques/methods ; Electrochemistry/methods ; Graphite/chemistry ; Humans ; Microelectrodes ; Oxidation-Reduction ; Platinum/chemistry ; Serum Albumin/chemistry ; Uric Acid/analysis ; Uric Acid/chemistry
Chemical Substances	Serum Albumin ; Uric Acid (268B43MJ25) ; Platinum (49DFR088MY) ; Graphite (7782-42-5) ; Ascorbic Acid (PQ6CK8PD0R) ; Bilirubin (RFM9X3LJ49)
Language	English
Publishing date	2014-12
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2014.07.009
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Article ; Online: Biomarkers and Donor Selection in Heart Transplantation.

Vorlat, Anne / De Hous, Nicolas / Vervaecke, Alexander J / Vermeulen, Tom / Van Craenenbroeck, Emeline / Heidbuchel, Hein / Rodrigus, Inez / Van Donink, Walter / Ancion, Arnaud / Van Cleemput, Johan / Van Hoof, Viviane O / Claeys, Marc J

Transplantation proceedings

2019 Volume 51, Issue 6, Page(s) 1673–1678

Abstract: Background: Previously, we showed that B-type natriuretic peptide (BNP) measured in the donor was related to cardiac performance after cardiac transplantation. The present study assesses the value of 3 biomarkers in the selection of donor hearts in a ... ...

Abstract	Background: Previously, we showed that B-type natriuretic peptide (BNP) measured in the donor was related to cardiac performance after cardiac transplantation. The present study assesses the value of 3 biomarkers in the selection of donor hearts in a larger cohort. Methods: Blood samples were prospectively obtained in 105 brain-dead patients scheduled for heart donation. BNP, soluble suppressor of tumorigenicity 2 (ST2), and troponin of heart donors were correlated with hemodynamic parameters early after transplantation as well as with the mortality of the recipients. Results: A significant inverse relationship was found between donor BNP measured at the time of donation and recipient cardiac index and cardiac output at day 13 post-transplantation (r = -0.31, P = .005, and r = -0.34, P = .0016, respectively). Logistic regression analysis-including BNP, ST2, and troponin-showed that donor BNP was a predictor of a poor cardiac index (< 2.2 L/min/m Conclusion: Donor BNP, but not donor ST2 or high-sensitivity troponin, provides information on the donor heart and early post-transplant performance, including 1-month mortality.
MeSH term(s)	Adult ; Biomarkers/blood ; Brain Death/blood ; Cardiac Output ; Donor Selection/methods ; Female ; Heart/physiopathology ; Heart Transplantation ; Hemodynamics ; Humans ; Interleukin-1 Receptor-Like 1 Protein/blood ; Male ; Middle Aged ; Natriuretic Peptide, Brain/blood ; Prospective Studies ; Sensitivity and Specificity ; Tissue Donors ; Transplants/physiopathology ; Treatment Outcome ; Troponin/blood
Chemical Substances	Biomarkers ; IL1RL1 protein, human ; Interleukin-1 Receptor-Like 1 Protein ; Troponin ; Natriuretic Peptide, Brain (114471-18-0)
Language	English
Publishing date	2019-07-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	82046-5
ISSN	1873-2623 ; 0041-1345
ISSN (online)	1873-2623
ISSN	0041-1345
DOI	10.1016/j.transproceed.2019.04.041
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Article ; Online: Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice.

Grootaert, Mandy O J / Schrijvers, Dorien M / Hermans, Marthe / Van Hoof, Viviane O / De Meyer, Guido R Y / Martinet, Wim

Oxidative medicine and cellular longevity

2016 Volume 2016, Page(s) 3087469

Abstract: Apoptosis of macrophages and vascular smooth muscle cells (VSMCs) in advanced atherosclerotic plaques contributes to plaque progression and instability. Caspase-3, a key executioner protease in the apoptotic pathway, has been identified in human and ... ...

Abstract	Apoptosis of macrophages and vascular smooth muscle cells (VSMCs) in advanced atherosclerotic plaques contributes to plaque progression and instability. Caspase-3, a key executioner protease in the apoptotic pathway, has been identified in human and mouse atherosclerotic plaques but its role in atherogenesis is not fully explored. We therefore investigated the impact of caspase-3 deletion on atherosclerosis by crossbreeding caspase-3 knockout (Casp3
MeSH term(s)	Animals ; Apolipoproteins E/deficiency ; Apoptosis ; Caspase 3/metabolism ; Mice ; Mice, Knockout ; Necrosis ; Plaque, Atherosclerotic/metabolism
Chemical Substances	Apolipoproteins E ; Caspase 3 (EC 3.4.22.-)
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article
ISSN	1942-0994
ISSN (online)	1942-0994
DOI	10.1155/2016/3087469
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Article ; Online: Belgian rare diseases plan in clinical pathology: identification of key biochemical diagnostic tests and establishment of reference laboratories and financing conditions.

Orphanet journal of rare diseases

2021 Volume 16, Issue 1, Page(s) 89

Abstract: Background: One objective of the Belgian Rare Diseases plan is to improve patients' management using phenotypic tests and, more specifically, the access to those tests by identifying the biochemical analyses used for rare diseases, developing new ... ...

Abstract	Background: One objective of the Belgian Rare Diseases plan is to improve patients' management using phenotypic tests and, more specifically, the access to those tests by identifying the biochemical analyses used for rare diseases, developing new financing conditions and establishing reference laboratories. Methods: A feasibility study was performed from May 2015 until August 2016 in order to select the financeable biochemical analyses, and, among them, those that should be performed by reference laboratories. This selection was based on an inventory of analyses used for rare diseases and a survey addressed to the Belgian laboratories of clinical pathology (investigating the annual analytical costs, volumes, turnaround times and the tests unavailable in Belgium and outsourced abroad). A proposal of financeable analyses, financing modalities, reference laboratories' scope and budget estimation was developed and submitted to the Belgian healthcare authorities. After its approval in December 2016, the implementation phase took place from January 2017 until December 2019. Results: In 2019, new reimbursement conditions have been published for 46 analyses and eighteen reference laboratories have been recognized. Collaborations have also been developed with 5 foreign laboratories in order to organize the outsourcing and financing of 9 analyses unavailable in Belgium. Conclusions: In the context of clinical pathology and rare diseases, this initiative enabled to identify unreimbursed analyses and to meet the most crucial financial needs. It also contributed to improve patients' management by establishing Belgian reference laboratories and foreign referral laboratories for highly-specific analyses and a permanent surveillance, quality and financing framework for those tests.
MeSH term(s)	Belgium ; Budgets ; Diagnostic Tests, Routine ; Humans ; Laboratories ; Rare Diseases/diagnosis
Language	English
Publishing date	2021-02-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1750-1172
ISSN (online)	1750-1172
DOI	10.1186/s13023-021-01728-1
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Article ; Online: Analytical performance of a platform for point-of-care CRP testing in adults consulting for lower respiratory tract infection in primary care.

Matheeussen, Veerle / Van Hoof, Viviane / Loens, Katherine / Lammens, Christine / Vanderstraeten, Anouk / Coenen, Samuel / Butler, Chris C / Little, Paul / Verheij, Theo J M / Goossens, Herman / Ieven, Margareta

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

2018 Volume 37, Issue 7, Page(s) 1319–1323

Abstract: C-reactive protein (CRP) is a biomarker widely used for disease severity assessment and treatment of inflammatory conditions. Point-of-care testing (POCT) devices should ideally be rapid and provide similar results to standard tests done in laboratories. ...

Abstract	C-reactive protein (CRP) is a biomarker widely used for disease severity assessment and treatment of inflammatory conditions. Point-of-care testing (POCT) devices should ideally be rapid and provide similar results to standard tests done in laboratories. Two thousand nine hundred twenty-two serum samples were obtained from adult patients presenting to primary care with symptoms of lower respiratory infection in a European diagnostic study. The analytic performance of the CRP QuikRead POCT device (Orion Diagnostica) was evaluated by comparing results with a central laboratory method (Dimension Vista, Siemens), with both tests performed in a laboratory setting. For a CRP cut-off concentration of ≥ 30 mg/L, the QuikRead test had a sensitivity of 92.2%, and specificity of 99.4%. The mean difference between the QuikRead and the central lab test was 0.4 mg/L. The slope of the Passing-Bablok regression was 0.94 (95% CI 0.93-0.95) indicating an underestimation of CRP levels of 6% by QuikRead. CRP estimates obtained from the QuikRead test correlate well with a central laboratory assay and the measurement displays low inter-assay variation. Therefore, the QuikRead test is a good candidate for CRP testing in primary care.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers/analysis ; C-Reactive Protein/analysis ; Diagnostic Tests, Routine/standards ; Female ; Humans ; Male ; Middle Aged ; Point-of-Care Testing/standards ; Respiratory Tract Infections/diagnosis ; Sensitivity and Specificity ; Young Adult
Chemical Substances	Biomarkers ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2018-05-10
Publishing country	Germany
Document type	Comparative Study ; Evaluation Studies ; Journal Article
ZDB-ID	603155-9
ISSN	1435-4373 ; 0934-9723 ; 0722-2211
ISSN (online)	1435-4373
ISSN	0934-9723 ; 0722-2211
DOI	10.1007/s10096-018-3253-3
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