Article ; Online: RNA-seq reveals more consistent reference genes for gene expression studies in human non-melanoma skin cancers
PeerJ, Vol 5, p e
2017 Volume 3631
Abstract: Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, we have utilised next generation RNA sequencing (RNA-seq) to analyse the transcriptome of a ... ...
| Abstract | Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, we have utilised next generation RNA sequencing (RNA-seq) to analyse the transcriptome of a panel of non-melanoma skin cancer lesions, identifying genes that are consistently expressed across all samples. Genes encoding ribosomal proteins were amongst the most stable in this dataset. Validation of this RNA-seq data was examined using qPCR to confirm the suitability of a set of highly stable genes for use as qPCR RGs. These genes will provide a valuable resource for the normalisation of qPCR data for the analysis of non-melanoma skin cancer. |
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| Keywords | RNA-seq ; Reference gene ; qPCR ; Non-melanoma skin cancer ; Medicine ; R ; Biology (General) ; QH301-705.5 |
| Language | English |
| Publishing date | 2017-08-01T00:00:00Z |
| Publisher | PeerJ Inc. |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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