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  1. Article: A Compact Imaging Platform for Conducting

    Anupom, Taslim / Vanapalli, Siva A

    Life (Basel, Switzerland)

    2023  Volume 13, Issue 1

    Abstract: The model ... ...

    Abstract The model organism
    Language English
    Publishing date 2023-01-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life13010200
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Microfluidic tapered aspirators for mechanical characterization of microgel beads.

    Uddin, Md Mezbah / Vanapalli, Siva A

    Soft matter

    2023  Volume 19, Issue 35, Page(s) 6641–6651

    Abstract: In this study, we report a microfluidic approach for the measurement of mechanical properties of spherical microgel beads. This technique is analogous to tapered micropipette aspiration, while harnessing the benefits of microfluidics. We fabricate ... ...

    Abstract In this study, we report a microfluidic approach for the measurement of mechanical properties of spherical microgel beads. This technique is analogous to tapered micropipette aspiration, while harnessing the benefits of microfluidics. We fabricate alginate-based microbeads and determine their mechanical properties using the microfluidic tapered aspirators. Individual microgel beads are aspirated and trapped in tapered channels, the deformed equilibrium shape is measured, and a stress balance is used to determine the Young's modulus. We investigate the effect of surface coating, taper angle, and bead diameter and find that the measured modulus is largely insensitive to these parameters. We show that the bead modulus increases with alginate concentration and follows a trend similar to that of the modulus measured using standard uniaxial compression. The critical pressure to squeeze out the beads from the tapered aspirators was found to depend on both the modulus and the bead diameter. Finally, we demonstrate how temporal changes in bead moduli due to enzymatic degradation of the hydrogel could be quantitatively determined. The results from this study highlight that the microfluidic tapered aspirators are a useful tool to measure hydrogel bead mechanics and have the potential to characterize dynamic changes in mechanical properties.
    Language English
    Publishing date 2023-09-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191476-X
    ISSN 1744-6848 ; 1744-683X
    ISSN (online) 1744-6848
    ISSN 1744-683X
    DOI 10.1039/d2sm01357f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Inertial focusing of particles and cells in the microfluidic labyrinth device: Role of sharp turns.

    Gangadhar, Anirudh / Vanapalli, Siva A

    Biomicrofluidics

    2022  Volume 16, Issue 4, Page(s) 44114

    Abstract: Inertial, size-based focusing was investigated in the microfluidic labyrinth device consisting of several U-shaped turns along with circular loops. Turns are associated with tight curvature and, therefore, induce strong Dean forces for separating ... ...

    Abstract Inertial, size-based focusing was investigated in the microfluidic labyrinth device consisting of several U-shaped turns along with circular loops. Turns are associated with tight curvature and, therefore, induce strong Dean forces for separating particles; however, systematic studies exploring this possibility do not exist. We characterized the focusing dynamics of different-sized rigid particles, cancer cells, and white blood cells over a range of fluid Reynolds numbers
    Language English
    Publishing date 2022-08-26
    Publishing country United States
    Document type Journal Article
    ISSN 1932-1058
    ISSN 1932-1058
    DOI 10.1063/5.0101582
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Deep learning assisted holography microscopy for in-flow enumeration of tumor cells in blood.

    Gangadhar, Anirudh / Sari-Sarraf, Hamed / Vanapalli, Siva A

    RSC advances

    2023  Volume 13, Issue 7, Page(s) 4222–4235

    Abstract: Currently, detection of circulating tumor cells (CTCs) in cancer patient blood samples relies on immunostaining, which does not provide access to live CTCs, limiting the breadth of CTC-based applications. Here, we take the first steps to address this ... ...

    Abstract Currently, detection of circulating tumor cells (CTCs) in cancer patient blood samples relies on immunostaining, which does not provide access to live CTCs, limiting the breadth of CTC-based applications. Here, we take the first steps to address this limitation, by demonstrating staining-free enumeration of tumor cells spiked into lysed blood samples using digital holographic microscopy (DHM), microfluidics and machine learning (ML). A 3D-printed module for laser assembly was developed to simplify the optical set up for holographic imaging of cells flowing through a sheath-based microfluidic device. Computational reconstruction of the holograms was performed to localize the cells in 3D and obtain the plane of best focus images to train deep learning models. We developed a custom-designed light-weight shallow Network dubbed s-Net and compared its performance against off-the-shelf CNN models including ResNet-50. The accuracy, sensitivity and specificity of the s-Net model was found to be higher than the off-the-shelf ML models. By applying an optimized decision threshold to mixed samples prepared
    Language English
    Publishing date 2023-02-02
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d2ra07972k
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Capillary imbibition of confined monodisperse emulsions in microfluidic channels.

    Norouzi Darabad, Masoud / Singha, Sagnik / Vanapalli, Siva A / Vaughn, Mark W / Blawzdziewicz, Jerzy

    Soft matter

    2024  

    Abstract: We study imbibition of a monodisperse emulsion into high-aspect ratio microfluidic channels with the ... ...

    Abstract We study imbibition of a monodisperse emulsion into high-aspect ratio microfluidic channels with the height
    Language English
    Publishing date 2024-04-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191476-X
    ISSN 1744-6848 ; 1744-683X
    ISSN (online) 1744-6848
    ISSN 1744-683X
    DOI 10.1039/d4sm00179f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: A nanosheet-based combination emulsifier system for bulk-scale production of emulsions with elongated droplets and long-term stability

    Anjum, Nishat / Chang, Ya-Wen / Vanapalli, Siva A.

    Colloids and surfaces. 2022 May 05, v. 640

    2022  

    Abstract: In this study, we investigate the emulsion type, droplet morphology and emulsion stability that can be achieved using a novel combination emulsifier system which consists of water-soluble α-zirconium phosphate tetrabutyl ammonium (α-ZrP-TBA) nanosheets ... ...

    Abstract In this study, we investigate the emulsion type, droplet morphology and emulsion stability that can be achieved using a novel combination emulsifier system which consists of water-soluble α-zirconium phosphate tetrabutyl ammonium (α-ZrP-TBA) nanosheets as the particulate emulsifier and Span 80 as the oil-soluble emulsifier, with the two emulsifiers present in distinct phases. We find that water-in-oil emulsions were formed for water volume fractions < 0.3, and oil-in-water (O/W) emulsions were formed for water volume fractions > 0.3. This phase inversion point was found to be the same irrespective of whether the emulsifier was only Span 80 or only nanosheet or their combination. Varying the concentrations of either surfactant or the nanosheet did not alter the phase inversion point. The emulsions with the greatest stability against creaming were formed using the combination emulsifier and were found to be the O/W type, that were stable for 3 months. Interestingly, we find that droplets in these stable emulsions were nonspherical, with the aspect ratio of droplets increasing with nanosheet and Span 80 concentration, suggesting a synergistic interaction between the two emulsifiers to create elongated droplets. We perform capillary neck thinning experiments to show that the combination emulsifier system offers unique interfacial properties that are different from either emulsifier alone, suggesting that interfacial jamming could occur to produce elongated droplets. Taken together, our results suggest a novel route to bulk-scale production of O/W emulsions that have long-term emulsion stability with unique droplet morphology.
    Keywords droplets ; emulsifiers ; emulsions ; nanosheets ; neck ; phosphates ; surfactants ; synergism ; tetrabutylammonium compounds ; water solubility
    Language English
    Dates of publication 2022-0505
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1500517-3
    ISSN 0927-7757
    ISSN 0927-7757
    DOI 10.1016/j.colsurfa.2022.128403
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Preface to the Special Topic: Microfluidics, Circulating Biomarkers and Cancer.

    Vanapalli, Siva A / Clair Hur, Soojung

    Biomicrofluidics

    2022  Volume 16, Issue 4, Page(s) 40401

    Language English
    Publishing date 2022-08-16
    Publishing country United States
    Document type Journal Article
    ISSN 1932-1058
    ISSN 1932-1058
    DOI 10.1063/5.0116803
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Comprehensive Profiling of Cancer-Associated Cells in the Blood of Breast Cancer Patients Undergoing Neoadjuvant Chemotherapy to Predict Pathological Complete Response.

    Pore, Adity A / Dhanasekara, Chathurika S / Navaid, Hunaiz Bin / Vanapalli, Siva A / Rahman, Rakhshanda Layeequr

    Bioengineering (Basel, Switzerland)

    2023  Volume 10, Issue 4

    Abstract: Neoadjuvant chemotherapy (NAC) can affect pathological complete response (pCR) in breast cancers; the resection that follows identifies patients with residual disease who are then offered second-line therapies. Circulating tumor cells (CTCs) and cancer- ... ...

    Abstract Neoadjuvant chemotherapy (NAC) can affect pathological complete response (pCR) in breast cancers; the resection that follows identifies patients with residual disease who are then offered second-line therapies. Circulating tumor cells (CTCs) and cancer-associated macrophage-like cells (CAMLs) in the blood can be used as potential biomarkers for predicting pCR before resection. CTCs are of epithelial origin that undergo epithelial-to-mesenchymal transition to become more motile and invasive, thereby leading to invasive mesenchymal cells that seed in distant organs, causing metastasis. Additionally, CAMLs in the blood of cancer patients are reported to either engulf or aid the transport of cancer cells to distant organs. To study these rare cancer-associated cells, we conducted a preliminary study where we collected blood from patients treated with NAC after obtaining their written and informed consent. Blood was collected before, during, and after NAC, and Labyrinth microfluidic technology was used to isolate CTCs and CAMLs. Demographic, tumor marker, and treatment response data were collected. Non-parametric tests were used to compare pCR and non-pCR groups. Univariate and multivariate models were used where CTCs and CAMLs were analyzed for predicting pCR. Sixty-three samples from 21 patients were analyzed. The median(IQR) pre-NAC total and mesenchymal CTC count/5 mL was lower in the pCR vs. non-pCR group [1(3.5) vs. 5(5.75);
    Language English
    Publishing date 2023-04-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2746191-9
    ISSN 2306-5354
    ISSN 2306-5354
    DOI 10.3390/bioengineering10040485
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: FHOD-1/profilin-mediated actin assembly protects sarcomeres against contraction-induced deformation in

    Kimmich, Michael J / Sundaramurthy, Sumana / Geary, Meaghan A / Lesanpezeshki, Leila / Yingling, Curtis V / Vanapalli, Siva A / Littlefield, Ryan S / Pruyne, David

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Formin HOmology Domain 2-containing (FHOD) proteins are a subfamily of actin-organizing formins important for striated muscle development in many animals. We showed previously that absence of the sole FHOD protein, FHOD-1, ... ...

    Abstract Formin HOmology Domain 2-containing (FHOD) proteins are a subfamily of actin-organizing formins important for striated muscle development in many animals. We showed previously that absence of the sole FHOD protein, FHOD-1, from
    Language English
    Publishing date 2024-04-03
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.02.29.582848
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  10. Article: Single-Cell Proliferation Microfluidic Device for High Throughput Investigation of Replicative Potential and Drug Resistance of Cancer Cells.

    Pore, Adity A / Kamyabi, Nabiollah / Bithi, Swastika S / Ahmmed, Shamim M / Vanapalli, Siva A

    Cellular and molecular bioengineering

    2023  Volume 16, Issue 5-6, Page(s) 443–457

    Abstract: Introduction: Cell proliferation represents a major hallmark of cancer biology, and manifests itself in the assessment of tumor growth, drug resistance and metastasis. Tracking cell proliferation or cell fate at the single-cell level can reveal ... ...

    Abstract Introduction: Cell proliferation represents a major hallmark of cancer biology, and manifests itself in the assessment of tumor growth, drug resistance and metastasis. Tracking cell proliferation or cell fate at the single-cell level can reveal phenotypic heterogeneity. However, characterization of cell proliferation is typically done in bulk assays which does not inform on cells that can proliferate under given environmental perturbations. Thus, there is a need for single-cell approaches that allow longitudinal tracking of the fate of a large number of individual cells to reveal diverse phenotypes.
    Methods: We fabricated a new microfluidic architecture for high efficiency capture of single tumor cells, with the capacity to monitor cell divisions across multiple daughter cells. This single-cell proliferation (SCP) device enabled the quantification of the fate of more than 1000 individual cancer cells longitudinally, allowing comprehensive profiling of the phenotypic heterogeneity that would be otherwise masked in standard cell proliferation assays. We characterized the efficiency of single cell capture and demonstrated the utility of the SCP device by exposing MCF-7 breast tumor cells to different doses of the chemotherapeutic agent doxorubicin.
    Results: The single cell trapping efficiency of the SCP device was found to be ~ 85%. At the low doses of doxorubicin (0.01 µM, 0.001 µM, 0.0001 µM), we observed that 50-80% of the drug-treated cells had undergone proliferation, and less than 10% of the cells do not proliferate. Additionally, we demonstrated the potential of the SCP device in circulating tumor cell applications where minimizing target cell loss is critical. We showed selective capture of breast tumor cells from a binary mixture of cells (tumor cells and white blood cells) that was isolated from blood processing. We successfully characterized the proliferation statistics of these captured cells despite their extremely low counts in the original binary suspension.
    Conclusions: The SCP device has significant potential for cancer research with the ability to quantify proliferation statistics of individual tumor cells, opening new avenues of investigation ranging from evaluating drug resistance of anti-cancer compounds to monitoring the replicative potential of patient-derived cells.
    Supplementary information: The online version contains supplementary material available at 10.1007/s12195-023-00773-z.
    Language English
    Publishing date 2023-07-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2416037-4
    ISSN 1865-5033 ; 1865-5025
    ISSN (online) 1865-5033
    ISSN 1865-5025
    DOI 10.1007/s12195-023-00773-z
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