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Article ; Online: Editorial: Parasite Polyamines.

Vannier-Santos, Marcos A

2017 Volume 23, Issue 23, Page(s) 3323–3324

Language	English
Publishing date	2017
Publishing country	Netherlands
Document type	Editorial
ZDB-ID	1304236-1
ISSN	1873-4286 ; 1381-6128
ISSN (online)	1873-4286
ISSN	1381-6128
DOI	10.2174/138161282323170927122509
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Article ; Online: Combination Therapy of Curcumin and Disulfiram Synergistically Inhibits the Growth of B16-F10 Melanoma Cells by Inducing Oxidative Stress.

Fontes, Sheila S / Nogueira, Mateus L / Dias, Rosane B / Rocha, Clarissa A Gurgel / Soares, Milena B P / Vannier-Santos, Marcos A / Bezerra, Daniel P

Biomolecules

2022 Volume 12, Issue 11

Abstract: Oxidative stress plays a central role in the pathophysiology of melanoma. Curcumin (CUR) is a polyphenolic phytochemical that stimulates reactive oxygen species (ROS) production, while disulfiram (DSS) is a US FDA-approved drug for the treatment of ... ...

Abstract	Oxidative stress plays a central role in the pathophysiology of melanoma. Curcumin (CUR) is a polyphenolic phytochemical that stimulates reactive oxygen species (ROS) production, while disulfiram (DSS) is a US FDA-approved drug for the treatment of alcoholism that can act by inhibiting the intracellular antioxidant system. Therefore, we hypothesized that they act synergistically against melanoma cells. Herein, we aimed to study the antitumor potential of the combination of CUR with DSS in B16-F10 melanoma cells using in vitro and in vivo models. The cytotoxic effects of different combination ratios of CUR and DSS were evaluated using the Alamar Blue method, allowing the production of isobolograms. Apoptosis detection, DNA fragmentation, cell cycle distribution, and mitochondrial superoxide levels were quantified by flow cytometry. Tumor development in vivo was evaluated using C57BL/6 mice bearing B16-F10 cells. The combinations ratios of 1:2, 1:3, and 2:3 showed synergic effects. B16-F10 cells treated with these combinations showed improved apoptotic cell death and DNA fragmentation. Enhanced mitochondrial superoxide levels were observed at combination ratios of 1:2 and 1:3, indicating increased oxidative stress. In vivo tumor growth inhibition for CUR (20 mg/kg), DSS (60 mg/kg), and their combination were 17.0%, 19.8%, and 28.8%, respectively. This study provided data on the potential cytotoxic activity of the combination of CUR with DSS and may provide a useful tool for the development of a therapeutic combination against melanoma.
MeSH term(s)	Mice ; Animals ; Curcumin/pharmacology ; Curcumin/therapeutic use ; Disulfiram/pharmacology ; Cell Line, Tumor ; Superoxides/metabolism ; Mice, Inbred C57BL ; Melanoma, Experimental/metabolism ; Apoptosis ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Oxidative Stress
Chemical Substances	Curcumin (IT942ZTH98) ; Disulfiram (TR3MLJ1UAI) ; Superoxides (11062-77-4) ; Antineoplastic Agents
Language	English
Publishing date	2022-10-31
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom12111600
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Role of Polyamines in Parasite Cell Architecture and Function.

Vannier-Santos, Marcos A / Suarez-Fontes, Ana M

Current pharmaceutical design

2017 Volume 23, Issue 23, Page(s) 3342–3358

Abstract: In the absence of accessible, effective vaccines, the fight against parasitic disease relies mostly on chemotherapy. Nevertheless, the considerable side effects, high costs and growing number of refractory cases comprise substantial drawbacks. Thus, the ... ...

Abstract	In the absence of accessible, effective vaccines, the fight against parasitic disease relies mostly on chemotherapy. Nevertheless, the considerable side effects, high costs and growing number of refractory cases comprise substantial drawbacks. Thus, the search for new antiparasitic compounds remains a high priority. The polyamine biosynthesis, conversion and transport pathways offer different targets for selective chemotherapy. Polyamine analogues and other antagonists may provide tools in the search for new lead compounds. Light and electron microscopy techniques may encompass valuable approaches to elucidate the possible mechanisms of action of different antiparasitic compounds, allowing the identification of subcellular target compartments, presumably establishing the basis for a more rational drug design and/or planning of therapeutic strategies.
Language	English
Publishing date	2017
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1304236-1
ISSN	1873-4286 ; 1381-6128
ISSN (online)	1873-4286
ISSN	1381-6128
DOI	10.2174/1381612823666170703163458
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Article ; Online: Cytochrome

Mesquita, Rafael D / Gaviraghi, Alessandro / Gonçalves, Renata L S / Vannier-Santos, Marcos A / Mignaco, Julio A / Fontes, Carlos Frederico L / Machado, Luciana E S F / Oliveira, Marcus F

Cells

2021 Volume 10, Issue 2

Abstract: Flight dispersal represents a key aspect of the evolutionary and ecological success of insects, allowing escape from predators, mating, and colonization of new niches. The huge energy demand posed by flight activity is essentially met by oxidative ... ...

Abstract	Flight dispersal represents a key aspect of the evolutionary and ecological success of insects, allowing escape from predators, mating, and colonization of new niches. The huge energy demand posed by flight activity is essentially met by oxidative phosphorylation (OXPHOS) in flight muscle mitochondria. In insects, mitochondrial ATP supply and oxidant production are regulated by several factors, including the energy demand exerted by changes in adenylate balance. Indeed, adenylate directly regulates OXPHOS by targeting both chemiosmotic ATP production and the activities of specific mitochondrial enzymes. In several organisms, cytochrome
MeSH term(s)	Animals ; Electron Transport Complex IV/metabolism ; Insecta ; Oxidation-Reduction ; Oxidative Phosphorylation
Chemical Substances	Electron Transport Complex IV (EC 1.9.3.1)
Language	English
Publishing date	2021-02-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells10020470
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Article ; Online: Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis.

Gomes-Junior, Rafael Araújo / da Silva, Roberto Santana / de Lima, Renata Galvão / Vannier-Santos, Marcos A

FEMS microbiology letters

2017 Volume 364, Issue 9

Abstract: Candidiasis, a major opportunistic mycosis caused by Candida sp., may comprise life-threatening systemic infections. The incidence of non-albicans species is rising, particularly in South America and they are frequently drug resistant, causing ... ...

Abstract	Candidiasis, a major opportunistic mycosis caused by Candida sp., may comprise life-threatening systemic infections. The incidence of non-albicans species is rising, particularly in South America and they are frequently drug resistant, causing unresponsive cases. Thus, novel antimycotic agents are required. Here we tested the antifungal activity of [RuIII(NH3)4catechol]+ complex (RuCat), approaching possible action mechanisms on fluconazole-resistant Candida tropicalis. RuCat significantly (P < 0.05) inhibited the growth and viability of C. tropicalis dose-dependently (IC50 20.3 μM). Cytotoxicity of RuCat upon murine splenocytes was lower (Selectivity Index = 16). Scanning electron microscopy analysis showed pseudohyphae formation, yeast aggregation and surface damage. RuCat-treated samples investigated by transmission electron microscopy showed melanin granule trafficking to cell surfaces and extracellular milieu. Surface-adherent membrane fragments and extracellular debris were also observed. RuCat treatment produced intense H2DCFDA labeling, indicating reactive oxygen species (ROS) production which caused increased lipoperoxidation. ROS are involved in the fungicidal effect as N-acetyl-L-cysteine completely restored cell viability. Calcofluor White chitin staining suggests that 70 or 140 μM RuCat treatment for 2 h affected cell-wall structure. PI labeling indicated necrotic cell death. The present data indicate that RuCat triggers ROS production, lipoperoxidation and cell surface damage, culminating in selective necrotic death of drug-resistant C. tropicalis.
Language	English
Publishing date	2017-05-01
Publishing country	England
Document type	Journal Article
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1093/femsle/fnx073
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Zs.A 1372: Show issues

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Article: Modulation of Human Immune Response by Fungal Biocontrol Agents.

Konstantinovas, Cibele / de Oliveira Mendes, Tiago A / Vannier-Santos, Marcos A / Lima-Santos, Jane

Frontiers in microbiology

2017 Volume 8, Page(s) 39

Abstract: Although the vast majority of biological control agents is generally regarded as safe for humans and environment, the increased exposure of agriculture workers, and consumer population to fungal substances may affect the immune system. Those compounds ... ...

Abstract	Although the vast majority of biological control agents is generally regarded as safe for humans and environment, the increased exposure of agriculture workers, and consumer population to fungal substances may affect the immune system. Those compounds may be associated with both intense stimulation, resulting in IgE-mediated allergy and immune downmodulation induced by molecules such as cyclosporin A and mycotoxins. This review discusses the potential effects of biocontrol fungal components on human immune responses, possibly associated to infectious, inflammatory diseases, and defective defenses.
Language	English
Publishing date	2017-02-03
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2017.00039
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Article ; Online: Transmission electron microscopy revealing the mechanism of action of photodynamic therapy on Trichomonas vaginalis.

Fonseca, Thaisa H S / Gomes, Jessica M S / Alacoque, Marina / Vannier-Santos, Marcos A / Gomes, Maria A / Busatti, Haendel G N O

Acta tropica

2018 Volume 190, Page(s) 112–118

Abstract: Trichomonas vaginalis is an amitochondrial parasite that causes human trichomoniasis. Despite metronidazole effectiveness, resistant cases are becoming more frequent. This scenario reveals the need to develop new therapeutic options. Photodynamic Therapy ...

Abstract	Trichomonas vaginalis is an amitochondrial parasite that causes human trichomoniasis. Despite metronidazole effectiveness, resistant cases are becoming more frequent. This scenario reveals the need to develop new therapeutic options. Photodynamic Therapy (PDT) is an experimental treatment that involves the activation of photosensitive substances and the generation of cytotoxic oxygen species and free radicals to promote the selective destruction of target tissues. In previous work, we identified an excellent in vitro PDT activity using methylene blue and light emitting diode against metronidazole sensitive and resistant strains of T. vaginalis. Here, we evaluated the efficacy of PDT in vivo and its high trichomonicidal activity was assessed through transmission electron microscopy. Female Balb/c mice were infected intravaginally with T. vaginalis trophozoites. On the third day of infection, methylene blue was introduced into the vaginal canal, which then received 68.1 J/cm
MeSH term(s)	Animals ; Female ; Humans ; Metronidazole/therapeutic use ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron, Transmission/methods ; Photochemotherapy ; Trichomonas Vaginitis/drug therapy ; Trichomonas vaginalis/drug effects ; Trichomonas vaginalis/ultrastructure
Chemical Substances	Metronidazole (140QMO216E)
Language	English
Publishing date	2018-11-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	210415-5
ISSN	1873-6254 ; 0001-706X
ISSN (online)	1873-6254
ISSN	0001-706X
DOI	10.1016/j.actatropica.2018.11.005
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Article ; Online: Parasites or cohabitants: cruel omnipresent usurpers or creative "éminences grises"?

Vannier-Santos, Marcos A / Lenzi, Henrique L

Journal of parasitology research

2011 Volume 2011, Page(s) 214174

Abstract: This paper presents many types of interplays between parasites and the host, showing the history of parasites, the effects of parasites on the outcome of wars, invasions, migrations, and on the development of numerous regions of the globe, and the impact ...

Abstract	This paper presents many types of interplays between parasites and the host, showing the history of parasites, the effects of parasites on the outcome of wars, invasions, migrations, and on the development of numerous regions of the globe, and the impact of parasitic diseases on the society and on the course of human evolution. It also emphasizes the pressing need to change the look at the parasitism phenomenon, proposing that the term "cohabitant" is more accurate than parasite, because every living being, from bacteria to mammals, is a consortium of living beings in the pangenome. Even the term parasitology should be replaced by cohabitology because there is no parasite alone and host alone: both together compose a new adaptive system: the parasitized-host or the cohabitant-cohabited being. It also suggests switching the old paradigm based on attrition and destruction, to a new one founded on adaptation and living together.
Keywords	covid19
Language	English
Publishing date	2011-07-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2563542-6
ISSN	2090-0031 ; 2090-0023
ISSN (online)	2090-0031
ISSN	2090-0023
DOI	10.1155/2011/214174
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Article ; Online: Cyperus articulatus

Nogueira, Mateus L / Lima, Emilly J S P de / Adrião, Asenate A X / Fontes, Sheila S / Silva, Valdenizia R / Santos, Luciano de S / Soares, Milena B P / Dias, Rosane B / Rocha, Clarissa A Gurgel / Costa, Emmanoel V / Silva, Felipe M A da / Vannier-Santos, Marcos A / Cardozo, Nállarett M D / Koolen, Hector H F / Bezerra, Daniel P

Molecules (Basel, Switzerland)

2020 Volume 25, Issue 11

Abstract: ... Cyperus ... ...

Abstract	Cyperus articulatus
MeSH term(s)	Animals ; Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis ; Breast Neoplasms/drug therapy ; Breast Neoplasms/pathology ; Cell Cycle Checkpoints/drug effects ; Cell Movement ; Cell Proliferation ; Cyperus/chemistry ; Female ; Humans ; Mice ; Mice, SCID ; Oils, Volatile/pharmacology ; Plant Extracts/pharmacology ; Plant Leaves/chemistry ; Rhizome/chemistry ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays
Chemical Substances	Antineoplastic Agents, Phytogenic ; Oils, Volatile ; Plant Extracts
Language	English
Publishing date	2020-06-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules25112687
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Article ; Online: Cardiac effect induced by Crotalus durissus cascavella venom: Morphofunctional evidence and mechanism of action.

Simões, Letícia O / Alves, Quiara L / Camargo, Samuel B / Araújo, Fênix A / Hora, Viviane R S / Jesus, Rafael L C / Barreto, Breno C / Macambira, Simone G / Soares, Milena B P / Meira, Cássio S / Aguiar, Márcio C / Couto, Ricardo D / Lomonte, Bruno / Menezes-Filho, José Evaldo / Cruz, Jader S / Vannier-Santos, Marcos A / Casais-E-Silva, Luciana L / Silva, Darizy F

Toxicology letters

2020 Volume 337, Page(s) 121–133

Abstract: Envenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. ...

Abstract	Envenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. To investigate the inotropic and chronotropic effects induced by Cdcas, studies were performed on the left and right atria. A series of tests were conducted to investigate whether the negative inotropic effect, induced by Cdcas, was related to cardiac damage. Cdcas venom (0.1-30 μg/mL) elicited a significant negative inotropic effect. The addition of Cdcas crude venom (7.5, 15 and 30 μg/mL) did not induce significant alterations in cell proliferation, nor in the enzymatic activity of total-CK and CKMB. Ultrastructural evaluation demonstrated that cardiac cells from isoproterenol and Cdcas groups revealed discreet swelling and displaced intermyofibrillar mitochondria with disorganization of the cristae. No change was observed in cardiac electrical activity in perfused isolated rat hearts with Cdcas. In addition, Cdcas reduced contractility in isolated cardiomyocytes from the rat left ventricle. The negative inotropic effect of Cdcas was reduced by l-NAME (100 μM), PTIO (100 μM), ODQ (10 μM) and KT5823 (1 μM), suggesting the participation of NO/cGMP/PKG pathway due to Cdcas. In non-anesthetized rats, Cdcas induced hypotension followed by bradycardia, the latter was also observed by ECG (anesthetized animals). Our results suggest that the negative inotropic effect induced by Cdcas venom is unrelated to cardiac toxicity, at least, at the concentrations tested; and occurs through of NO/cGMP/PKG pathway, likely leading to hypotension and bradycardia when administered in vivo.
MeSH term(s)	Animals ; Arterial Pressure/drug effects ; Cardiotonic Agents/toxicity ; Cell Proliferation/drug effects ; Creatine Kinase/drug effects ; Creatine Kinase/metabolism ; Crotalid Venoms/antagonists & inhibitors ; Crotalid Venoms/chemistry ; Crotalid Venoms/toxicity ; Crotalus ; Heart/drug effects ; Heart Atria/drug effects ; Heart Rate/drug effects ; Heart Ventricles/cytology ; Heart Ventricles/drug effects ; In Vitro Techniques ; Male ; Mitochondria, Heart/drug effects ; Myocardial Contraction/drug effects ; Myocardium/enzymology ; Myocardium/pathology ; Myocardium/ultrastructure ; Myocytes, Cardiac/drug effects ; Rats ; Rats, Wistar ; Snake Bites
Chemical Substances	Cardiotonic Agents ; Crotalid Venoms ; Creatine Kinase (EC 2.7.3.2)
Language	English
Publishing date	2020-11-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	433788-8
ISSN	1879-3169 ; 0378-4274
ISSN (online)	1879-3169
ISSN	0378-4274
DOI	10.1016/j.toxlet.2020.11.019
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