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  1. Article ; Online: Carcinocythemia in metastatic breast cancer detected by high fluorescent signals on an automated Sysmex XN-9100 hematological cell counter.

    Vanrenterghem, Marthe / Mylemans, Marnix / Tousseyn, Thomas / Boeckx, Nancy

    International journal of laboratory hematology

    2023  Volume 45, Issue 5, Page(s) 625–627

    MeSH term(s) Humans ; Female ; Breast Neoplasms/diagnosis ; Automation, Laboratory ; Hematology
    Language English
    Publishing date 2023-06-05
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.14112
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Laboratory workflow for optimizing leukocyte count and differential in synovial fluids on Sysmex XN-1000.

    Vanrenterghem, Marthe / Dom, Julie / Boeckx, Nancy / Depypere, Melissa / Frans, Glynis / Vles, Georges / Van Laer, Christine

    International journal of laboratory hematology

    2024  

    Abstract: Introduction: Falsely elevated synovial white blood cell (WBC) counts using automated hematology analyzers have been reported particularly in the setting of joint arthroplasty. We evaluated the implementation of a laboratory workflow based on Sysmex XN- ... ...

    Abstract Introduction: Falsely elevated synovial white blood cell (WBC) counts using automated hematology analyzers have been reported particularly in the setting of joint arthroplasty. We evaluated the implementation of a laboratory workflow based on Sysmex XN-1000-automated cell counting and scattergram interpretation.
    Methods: WBC and differential were measured for 76 synovial fluid samples (29 native joints and 47 with joint arthroplasties) with Sysmex XN-1000 and manual methods. All scattergrams were evaluated for possible incorrect WBC and/or differential according to our implemented workflow. A specific finding was the "banana-shape" scattergram, which indicates possible interferences. The European Bone & Joint Infection Society (EBJIS) criteria were applied to identify possible prosthetic joint infections (PJIs) in patients with joint arthroplasties.
    Results: Correlation between automated and manual WBC counts, calculated for samples with WBC count <50 000/μL, was higher for native joints (r = 0.938) compared with patients known with arthroplasty (r = 0.906). Scattergrams classified as OK showed overall a higher correlation compared with scattergrams, which were interpreted as NOT OK. "Banana-shape" scattergrams (n = 19) showed falsely elevated automated WBC count, and the patterns were mainly seen in prosthesis patients (17/19 [89%]). Six of 47 (13%) patients with joint arthroplasties were reclassified from "confirmed" to "unlikely" PJI according to the EBJIS criteria.
    Conclusion: Our workflow based on scattergram interpretation resulted in accurate WBC counts in synovial fluid using automated/and or manual methods. It is important to identify the presence of "banana-shape" scattergrams to avoid overestimated automated WBC counts. Overall, automated synovial WBC count can be used, even for patients with arthroplasty, but after visual inspection of the scattergram to exclude possible interferences.
    Language English
    Publishing date 2024-03-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.14274
    Database MEDical Literature Analysis and Retrieval System OnLINE

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