Article: Quality assessment of a serum and xenofree medium for the expansion of human GMP-grade mesenchymal stromal cells.
2022 Volume 10, Page(s) e13391
Abstract: Background: Cell-based therapies are emerging as a viable modality to treat challenging diseases, resulting in an increasing demand for their large-scale, high-quality production. Production facilities face the issue of batch-to-batch consistency while ... ...
| Abstract | Background: Cell-based therapies are emerging as a viable modality to treat challenging diseases, resulting in an increasing demand for their large-scale, high-quality production. Production facilities face the issue of batch-to-batch consistency while producing a safe and efficient cell-based product. Controlling culture conditions and particularly media composition is a key factor of success in this challenge. Serum and Xeno-Free Media (SXFM) represent an interesting option to achieve this goal. By reducing batch to batch variability, they increase Good Manufacturing Practices (GMP)-compliance and safety regarding xenogenic transmission, as compared to fetal bovine serum (FBS) supplemented-media or human platelet lysate supplemented medium. Methods: In this study, the isolation, expansion and characteristics including the anti-inflammatory function of human mesenchymal stromal cells (MSC) are compared after culture in MEM Results: We showed that MSC derived from human bone-marrow and adipose tissue can be successfully isolated and expanded in this SXFM. Number and size of Colony-Forming Unit fibroblast (CFU-F) is increased compared to cells cultivated in hCPL medium. All cells retained a CD90 Conclusions: The use of MSC-Brew GMP Medium can therefore be considered for clinical bioprocesses as a safe and efficient substitute for hCPL media. |
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| Language | English |
| Publishing date | 2022-05-30 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2703241-3 |
| ISSN | 2167-8359 |
| ISSN | 2167-8359 |
| DOI | 10.7717/peerj.13391 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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