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  1. Article ; Online: Changes in physical activity and alimentation habits during the initial phase of the covid-19 pandemic

    Daiana Carvalho Borges / Vanusa Pousada da Hora / Rodrigo Dalke Meucci / Carlos Castilho de Barros / Ines Claudia Schadock

    Revista Brasileira de Prescrição e Fisiologia do Exercício, Vol 16, Iss 103, Pp 287-

    a descriptive transversal study

    2023  Volume 295

    Abstract: Background: Physical exercise and healthy alimentation are considered the two basic columns for a healthy life. This study, objected to describe the impact of physical distancing in the initial phase of the COVID-19 pandemic on these habits in an adult ... ...

    Abstract Background: Physical exercise and healthy alimentation are considered the two basic columns for a healthy life. This study, objected to describe the impact of physical distancing in the initial phase of the COVID-19 pandemic on these habits in an adult population well known for its high prevalence of overweight and its associated chronic diseases in Brazil. Materials and Method: From May to July 2020, a digital survey was released, questioning about general health, diet and physical activity before and during decreed pandemic restrictions. Results: The majority (56%) of the 1739 respondents fitted the WHO classification criteria for overweight and obesity, 42% reported chronic morbidities and increased body weight since March 2020 (48%). The number of people not practicing any physical exercise had doubled in only three months (from 25% to 48% after March 2020) and time spend sedentary at work (38%) as well as in work-free times had increased (76%). Alimentation routines adapted partly worrisome habits like introducing more vespertine snacks between main meals but also positive changes like cooking at home (53%), and higher consumption of natural products (34%). Conclusion: Considering the age distribution of the participants (67% < 40 years of age), access to digital media, high educational level (77% with university degree), good to excellent basic sanitation (97%), the observed habit changes in only three months are concerning. It shows that even socially privileged people struggle to adapt healthy routines in times of crisis.
    Keywords sars-cov-2 ; obesity ; physical activity ; sedentary behavior ; nutrition ; Sports medicine ; RC1200-1245
    Subject code 796
    Language Portuguese
    Publishing date 2023-01-01T00:00:00Z
    Publisher Instituto Brasileiro de Pesquisa e Ensino em Fisiologia do Exercício
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Impact of hepatitis C virus eradication with direct-acting antivirals on glycidic metabolism

    Jucéli Márcia Hendges Sparvoli / Antonio Cardoso Sparvoli / Samuel de Carvalho Dumith / Afonso Alexandre Pereira / Ana Luisa Machado de Paula / Laís Garcia / Vanusa Belarmino / Vanusa Pousada da Hora / Ana Maria Barral de Martínez / Carla Vitola Gonçalves

    Archives of Endocrinology and Metabolism, Vol 67, Iss 3, Pp 314-

    2023  Volume 322

    Abstract: ABSTRACT Objective: To compare the glucose metabolism of patients with chronic hepatitis C virus infection treated with direct-acting antivirals (DAAs) in pretreatment and sustained viral response (SVR) periods. Materials and methods: This was an ... ...

    Abstract ABSTRACT Objective: To compare the glucose metabolism of patients with chronic hepatitis C virus infection treated with direct-acting antivirals (DAAs) in pretreatment and sustained viral response (SVR) periods. Materials and methods: This was an intervention pre-post study of 273 patients with chronic hepatitis C virus infection treated with DAAs from March 2018 to December 2019. Glycidic metabolism was evaluated through homeostasis model assessment (HOMA) – insulin resistance (IR) and HOMA-β indices and assessments of insulinemia and HbA1c levels. These parameters were analyzed with a T test by paired comparison of the means of the variables and Wilcoxon's test paired for the median; in the variables with an abnormal distribution, the Z score was generated for the mean in both the pretreatment and SVR periods. Statistical significance was considered at p ≤ 0.05. Results: Among 273 participants, 125 (45.8%) had prediabetes, and 50 (18.3%) had diabetes. In SVR, there was a significant increase in platelets, albumin, alkaline phosphatase, cholesterol and triglycerides and a significant decrease in aspartate aminotransferase, alanine aminotransferase, gamma GT and bilirubin. The HOMA-IR and HOMA-β indices increased in SVR from 1.95 to 2.29 (p = 0.087) and 71.20 to 82.60 (p = 0.001), respectively. Insulinemia increased from 7.60 μU/mL to 8.90 μU/mL (p = 0.011). HbA1c decreased from 5.6 to 5.4 (p < 0.001). Among patients with prediabetes and those with diabetes, the reduction in HbA1c values was significant (p = 0.006 and p = 0.026, respectively). Conclusion: SVR significantly impacts and leads to improvement in glucose metabolism in patients with chronic liver disease induced by hepatitis C virus.
    Keywords Insulin resistance ; direct-acting antivirals ; diabetes mellitus ; hepatitis C virus ; sustained viral response ; Medicine ; R ; Diseases of the endocrine glands. Clinical endocrinology ; RC648-665
    Subject code 616
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher Brazilian Society of Endocrinology and Metabolism
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Laboratory diagnosis for Covid-19

    Juliana Lemos Dal Pizzol / Vanusa Pousada da Hora / Ana Júlia Reis / Júlia Vianna / Ivy Ramis / Andrea von Groll / Pedro Almeida da Silva

    Revista da Sociedade Brasileira de Medicina Tropical, Vol

    A mini-review

    2020  Volume 53

    Abstract: Abstract Coronavirus disease (COVID-19) is a pandemic caused by a new coronavirus, called SARS-CoV-2. This disease was first identified in December 2019 and rapidly developed into a challenge to the public health systems around the world. In the absence ... ...

    Abstract Abstract Coronavirus disease (COVID-19) is a pandemic caused by a new coronavirus, called SARS-CoV-2. This disease was first identified in December 2019 and rapidly developed into a challenge to the public health systems around the world. In the absence of a vaccine and specific therapies, disease control and promotion of patient health are strongly dependent on a rapid and accurate diagnosis. This review describes the main laboratory approaches to making a diagnosis of COVID-19 and identifying those previously infected with SARS-CoV-2.
    Keywords COVID-19 ; Diagnostic ; Serology ; Molecular ; Biomarkers ; Arctic medicine. Tropical medicine ; RC955-962
    Language English
    Publishing date 2020-08-01T00:00:00Z
    Publisher Sociedade Brasileira de Medicina Tropical (SBMT)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Protective effect of the probiotic Lactobacillus acidophilus ATCC 4356 in BALB/c mice infected with Toxocara canis

    Priscila Silva Cadore / Débora Liliane Walcher / Nicholas Frota Gonçalves Correia de Sousa / Lourdes Helena Rodrigues Martins / Vanusa Pousada da Hora / Andrea Von Groll / Micaele Quintana de Moura / Maria Elisabeth Aires Berne / Luciana Farias da Costa de Avila / Carlos James Scaini

    Revista do Instituto de Medicina Tropical de São Paulo, Vol

    2021  Volume 63

    Abstract: ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ ... ...

    Abstract ABSTRACT Human toxocariasis consists of chronic tissue parasitosis that is difficult to treat and control. This study aimed to evaluate the action of the probiotic Lactobacillus acidophilus ATCC 4356 on larvae of Toxocara canis and the effect of IFN-γ cytokine on parasite-host in vivo (1.109 CFU) and in vitro (1.106, 1.107, 1.108, 1.109 CFU) interactions. Four groups of six BALB/c mice were formed: G1 - L. acidophilus supplementation and T. canis infection; G2 - T. canis infection; G3 - L. acidophilus supplementation; and G4 - PBS administration. Mice were intragastrically suplemented with probiotics for 15 days before inoculation and 48 h after inoculation with 100 T. canis eggs. The inoculation of T. canis was also perfomed intragastrically. The recovery of larvae took place through digestion of liver and lung tissues; the evaluation of IFN-γ gene transcription in leukocytes was performed by qPCR. The in vitro test consisted of incubating the probiotic with T. canis larvae. The supplementation of probiotics produced a reduction of 57.7% (p = 0.025) in the intensity of infection of T. canis larvae in mice, whereas in the in vitro test, there was no larvicidal effect. In addition, a decrease in the IFN-γ gene transcription was observed in both, T. canis-infected and uninfected mice, regardless of whether or not they received supplementation. The probiotic L. acidophilus ATCC 4356 reduced T. canis infection intensity in mice, however, the probiotic did not have a direct effect on larvae, demonstrating the need of interaction with the host for the beneficial effect of the probiotic to occur. Yet, the proinflammatory cytokine IFN-γ did not apparently contributed to the observed beneficial effect of probiotics.
    Keywords Toxocariasis ; Probiotic ; Innate immunity ; Cytokine ; INF-γ ; Arctic medicine. Tropical medicine ; RC955-962
    Subject code 570
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Universidade de São Paulo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil

    Ana Paula Neuschrank Albano / Gabriel Baracy Klafke / Tchana Martinez Brandolt / Vanusa Pousada Da Hora / Carlos Eduardo Wayne Nogueira / Melissa Orzechowski Xavier / Mário Carlos Araújo Meireles

    Brazilian Journal of Microbiology, Vol 46, Iss 2, Pp 513-

    2015  Volume 517

    Abstract: Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. ... ...

    Abstract Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.
    Keywords ELISA ; gp-43 ; protein G-peroxidase ; paracoccidioidomycosis ; Microbiology ; QR1-502 ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2015-06-01T00:00:00Z
    Publisher Sociedade Brasileira de Microbiologia
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: PREVALENCE OF HERPES SIMPLEX VIRUS TYPE 2 AND RISK FACTORS ASSOCIATED WITH THIS INFECTION IN WOMEN IN SOUTHERN BRAZIL

    Thais Duquia Moraes Caldeira / Carla Vitola Goncalves / Gisele Rodrigues de Oliveira / Tania Vieira da Fonseca / Regina Goncalves / Clair Teixeira do Amaral / Vanusa Pousada da Hora / Ana Maria Barral de Martinez

    Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 5, Pp 315-

    2013  Volume 321

    Abstract: SUMMARY The herpes simplex virus type 2 (HVS-2) is the most prevalent infection worldwide. It is a cofactor in the acquisition of human immunodeficiency virus (HIV) and the persistence of human papillomavirus (HPV). This study evaluated the prevalence of ...

    Abstract SUMMARY The herpes simplex virus type 2 (HVS-2) is the most prevalent infection worldwide. It is a cofactor in the acquisition of human immunodeficiency virus (HIV) and the persistence of human papillomavirus (HPV). This study evaluated the prevalence of HSV-2, using the polymerase chain reaction (PCR), and associated factors in patients treated at the Federal University of Rio Grande (FURG) and Basic Health Units (BHU) in Rio Grande, Brazil. The observed prevalence of HSV-2 was 15.6%. Among the 302 women studied, 158 had received assistance in BHU and 144 were treated at FURG. The prevalence of HSV-2 in these groups was 10.8% and 20.8%, respectively, RR 1.9 and p = 0.012. Knowledge about the Pap smear, and the presence of lesions showed no association with HSV-2 infection. Multivariate analysis showed that the variable that most influenced the risk of HSV-2 infection was the presence of HIV infection, with a relative risk of 1.9 and p = 0.04. Discussion: Genital ulcers are an important entry point for HIV, and condom use is an important strategy to reduce transmission of HIV and HSV-2.
    Keywords Human Herpesvirus 2 ; Polymerase Chain Reaction ; Human Papillomavirus ; HIV Infections ; Arctic medicine. Tropical medicine ; RC955-962
    Subject code 616
    Language English
    Publishing date 2013-09-01T00:00:00Z
    Publisher Universidade de São Paulo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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