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  1. Article ; Online: Correlation of dynamic impact testing, histopathology and visual macroscopic assessment in human osteoarthritic cartilage.

    Kos, Petr / Varga, Ferdinand / Handl, Milan / Kautzner, Jakub / Chudáček, Václav / Držík, Milan / Povýšil, Ctibor / Trč, Tomáš / Amler, Evžen / Hanus, Martin

    International orthopaedics

    2011  Volume 35, Issue 11, Page(s) 1733–1739

    Abstract: Objective: Improved staging of cartilage degeneration is required, particularly during the early stages. We correlated mechanical properties with histological and macroscopic findings.: Methods: One hundred and twenty cartilage samples were obtained ... ...

    Abstract Objective: Improved staging of cartilage degeneration is required, particularly during the early stages. We correlated mechanical properties with histological and macroscopic findings.
    Methods: One hundred and twenty cartilage samples were obtained during total knee arthroplasty. Two adjacent plugs were harvested--one for histological classification and one for macroscopic and biomechanical purposes. Dynamic impact testing was performed; normal stress, dissipated energy (∆E), tangent modulus and stiffness were evaluated.
    Results: Samples were classified according to six categories of the ICRS histological scale. Mechanical characteristics revealing significant differences between the groups (p < 0.01) were specific damping and related absolute ∆E. A significant correlation was found between the macroscopic score and specific damping, as well as absolute and relative ∆E (p < 0.01). A strong relation was revealed between relative ∆E and cartilage thickness (p < 0.001; R (2) = 0.69).
    Conclusions: Only ∆E correlated with the condition of the cartilage--the value increased with decreasing quality-and is the most suitable characteristic. This change appears substantial in initial stages of cartilage deterioration.
    MeSH term(s) Aged ; Aged, 80 and over ; Biomechanical Phenomena ; Cartilage, Articular/pathology ; Compressive Strength ; Elasticity ; Female ; Humans ; Male ; Middle Aged ; Osteoarthritis, Knee/pathology ; Osteoarthritis, Knee/physiopathology ; Osteoarthritis, Knee/surgery ; Stress, Mechanical
    Language English
    Publishing date 2011-01-18
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80384-4
    ISSN 1432-5195 ; 0341-2695
    ISSN (online) 1432-5195
    ISSN 0341-2695
    DOI 10.1007/s00264-010-1195-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1324: Show issues Location:
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  2. Article ; Online: Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee.

    Filová, Eva / Jelínek, Frantisek / Handl, Milan / Lytvynets, Andriy / Rampichová, Michala / Varga, Ferdinand / Cinátl, Jaroslav / Soukup, Tomás / Trc, Tomás / Amler, Evzen

    Journal of biomedical materials research. Part B, Applied biomaterials

    2008  Volume 87, Issue 2, Page(s) 415–424

    Abstract: A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea. The biophysical properties of the composite scaffold were similar to native ... ...

    Abstract A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea. The biophysical properties of the composite scaffold were similar to native cartilage. The macroscopic, histological, and immunohistochemical analysis of the regenerated tissue from cell-seeded scaffolds was performed 6 weeks after the implantation and predominantly showed formation of hyaline cartilage accompanied by production of glycosaminoglycans and type II collagen with minor fibro-cartilage production. Implanted scaffolds without cells healed predominantly as fibro-cartilage, although glycosaminoglycans and type II collagen, which form hyaline cartilage, were also observed. On the other hand, fibro-cartilage or fibrous tissue or both were only formed in the defects without scaffold. The new composite scaffold containing collagen type I, hyaluronan, and fibrin, seeded with autologous chondrocytes and implanted into rabbit femoral trochlea, was found to be highly effective in cartilage repair after only 6 weeks. The new composite scaffold can therefore enhance cartilage regeneration of osteochondral defects, by the supporting of the hyaline cartilage formation.
    MeSH term(s) Animals ; Biomechanical Phenomena ; Cell Survival ; Cells, Cultured ; Chondrocytes/cytology ; Collagen Type I ; Fibrin ; Hyaluronic Acid ; Knee Injuries/surgery ; Male ; Materials Testing ; Microscopy, Electron ; Prostheses and Implants ; Rabbits
    Chemical Substances Collagen Type I ; Fibrin (9001-31-4) ; Hyaluronic Acid (9004-61-9)
    Language English
    Publishing date 2008-04-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2099992-6
    ISSN 1552-4981 ; 1552-4973 ; 0021-9304
    ISSN (online) 1552-4981
    ISSN 1552-4973 ; 0021-9304
    DOI 10.1002/jbm.b.31119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 6196: Show issues Location:
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  3. Article: Reconstruction of the anterior cruciate ligament: dynamic strain evaluation of the graft.

    Handl, Milan / Drzík, Milan / Cerulli, Giuliano / Povýsil, Ctibor / Chlpík, Juraj / Varga, Ferdinand / Amler, Evzen / Trc, Tomás

    Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA

    2007  Volume 15, Issue 3, Page(s) 233–241

    Abstract: The study is focused on the biomechanical aspects of the anterior cruciate ligament (ACL) reconstruction procedures with an emphasis on evaluating the dynamic strain of materials commonly used for this purpose. Separate and multiple, equally tensioned ... ...

    Abstract The study is focused on the biomechanical aspects of the anterior cruciate ligament (ACL) reconstruction procedures with an emphasis on evaluating the dynamic strain of materials commonly used for this purpose. Separate and multiple, equally tensioned strands of hamstring grafts used for the reconstruction of the ACL were biomechanically tested and compared to original ACL and bone-patellar tendon-bone (BPTB) grafts, using tissue samples from cadavers. The study was focused on measuring such material properties as the strength, stiffness, maximum load, and elongation at maximum load of the original ACL, BPTB graft, and single tendon hamstring (gracilis and semitendinosus) grafts, continued by double strands and finally by four-strand graft (STG) evaluation. Fresh-frozen cadaveric knees were used, which had been clamped and tensioned equally. The measurement was performed by drop-weight testing, using a Laser Doppler Vibrometer as a basic sensor of the dynamic movements of the gripping clamps, with parallel correlation by a piezoelectric transducer. The grafts for experiments were obtained from 21-paired knees. The measurement was performed at room temperature (21 degrees C) after 24 h of thawing at 4 degrees C. All the specimens were measured for their response to the dynamic tensile load. The maximum strength values were obtained and calculated for the appropriate section area of the specimen. The tensioned strands of the original ACL showed a maximum average load of 1,246 +/- 243 N in the section area of about 30 mm(2) (max. stress 41.3 MPa); the strands of BPTB grafts showed values of 3,855 +/- 550 N in the section area of 80 mm(2) (max. stress 40.6 MPa); the gracilis tendons showed 925 +/- 127 N in the section area of 10 mm(2) (max. stress 95.1 MPa) and the semitendinosuss yielded a result of 2,050 +/- 159 N in the area of 20 mm(2) (max. stress 88.7 MPa). Of all the materials, the original ACL have the lowest strength and stiffness in respect of their biomechanical properties. BPTB grafts showed a slightly higher value of maximum stress, while both the gracilis and semitendinosus tendons showed double the value of maximum load per section area-tensile stress. Two- and four- combined hamstring strands clamped together and equally tensioned with a drop-weight had the combined tensile strength properties of the individual strands within the estimated range of measurement errors. No significant changes in maximum loads/stresses were observed under impact loading conditions. The results of this study demonstrate that equally tensioned four-strand hamstring-tendon grafts have higher initial tensile properties than those in other varieties of samples. From a biomechanical point of view, they seem to be a reasonable alternative procedure for ACL reconstruction.
    MeSH term(s) Aged ; Aged, 80 and over ; Anterior Cruciate Ligament/physiology ; Anterior Cruciate Ligament/surgery ; Bone-Patellar Tendon-Bone Grafting ; Cadaver ; Humans ; Middle Aged ; Stress, Mechanical ; Tendons/transplantation ; Tensile Strength ; Transducers
    Language English
    Publishing date 2007-03
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1159064-6
    ISSN 1433-7347 ; 0942-2056
    ISSN (online) 1433-7347
    ISSN 0942-2056
    DOI 10.1007/s00167-006-0175-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3742: Show issues Location:
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  4. Article ; Online: Fibrin/hyaluronic acid composite hydrogels as appropriate scaffolds for in vivo artificial cartilage implantation.

    Rampichová, Michala / Filová, Eva / Varga, Ferdinand / Lytvynets, Andriy / Prosecká, Eva / Koláčná, Lucie / Motlík, Jan / Nečas, Alois / Vajner, Luděk / Uhlík, Jiří / Amler, Evžen

    ASAIO journal (American Society for Artificial Internal Organs : 1992)

    2010  Volume 56, Issue 6, Page(s) 563–568

    Abstract: Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation ... ...

    Abstract Hydrogels prepared from a mixture of fibrin and high-molecular weight (MW) hyaluronic acid (HA) were found to be suitable scaffolds for chondrocyte seeding and pig knee cartilage regeneration. Collagen in the hydrogels is not necessary for the formation of biomechanically stable tissue. Regenerated cartilage showed very good biomechanical and histological properties only 6 months after implantation. Notably, the quality of the healing process was dependent on the initial chondrocyte concentration of the scaffolds. These experiments were performed according to good laboratory practice (GLP).
    MeSH term(s) Animals ; Biocompatible Materials/chemistry ; Biomechanical Phenomena ; Cartilage/physiology ; Cartilage/surgery ; Chondrocytes/cytology ; Chondrocytes/physiology ; Chondrogenesis ; Fibrin/chemistry ; Hyaluronic Acid/chemistry ; Hydrogels ; Male ; Materials Testing ; Prostheses and Implants ; Regeneration ; Swine ; Swine, Miniature ; Tissue Engineering ; Tissue Scaffolds/chemistry
    Chemical Substances Biocompatible Materials ; Hydrogels ; Fibrin (9001-31-4) ; Hyaluronic Acid (9004-61-9)
    Language English
    Publishing date 2010-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 759982-1
    ISSN 1538-943X ; 0162-1432 ; 1058-2916
    ISSN (online) 1538-943X
    ISSN 0162-1432 ; 1058-2916
    DOI 10.1097/MAT.0b013e3181fcbe24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Uk I Zs.199: Show issues Location:
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    Zs.B 2435: Show issues Location:
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