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Article ; Online: Cytokine Profile in Early Infection by

Bavia, Lorena / de Castro, Íris A / Amano, Mariane Tami / da Silva, Ana Maria Gonçalves / Vasconcellos, Silvio Arruda / Isaac, Lourdes

Journal of immunology research

2019 Volume 2019, Page(s) 1892508

Abstract: Leptospirosis is considered a neglected disease with an estimated more than one million cases every year. Since rodents are at the same time the main reservoir and generally asymptomatic ... ...

Abstract	Leptospirosis is considered a neglected disease with an estimated more than one million cases every year. Since rodents are at the same time the main reservoir and generally asymptomatic to
MeSH term(s)	Animals ; Biomarkers ; Biopsy ; Cytokines/blood ; Cytokines/metabolism ; Disease Models, Animal ; Host-Pathogen Interactions ; Leptospira interrogans ; Leptospirosis/blood ; Leptospirosis/metabolism ; Leptospirosis/microbiology ; Leptospirosis/pathology ; Leukocyte Count ; Liver/metabolism ; Liver/microbiology ; Liver/pathology ; Mice ; Organ Specificity ; Time Factors
Chemical Substances	Biomarkers ; Cytokines
Language	English
Publishing date	2019-10-07
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2817541-4
ISSN	2314-7156 ; 2314-8861
ISSN (online)	2314-7156
ISSN	2314-8861
DOI	10.1155/2019/1892508
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Article ; Online: Binding of human complement C1 sterase inhibitor to Leptospira spp.

Breda, Leandro Carvalho Dantas / Vasconcellos, Silvio Arruda / de Moraes Vasconcelos, Dewton / Isaac, Lourdes

Immunobiology

2017 Volume 223, Issue 2, Page(s) 183–190

Abstract: Leptospirosis is an important zoonosis of global importance caused by bacteria Leptospira spp. Pathogenic Leptospira is resistant to Complement System killing while non-pathogenic Leptospira is rapidly killed by exposure to normal human serum (NHS). ... ...

Abstract	Leptospirosis is an important zoonosis of global importance caused by bacteria Leptospira spp. Pathogenic Leptospira is resistant to Complement System killing while non-pathogenic Leptospira is rapidly killed by exposure to normal human serum (NHS). Pathogenic Leptospira interact with Complement Regulators such as Factor H, C4b binding protein and Vitronectin avoiding Complement activation and killing by Alternative and Classical Pathways. One important regulator is C1-inhibitor (C1INH) that interacts with C1s or MASPs controlling the cleavage of C4 and C2 molecules, thereby inhibiting the activation of the Classical and Lectin Pathways. In this study, we demonstrate that attenuated, saprophytic and pathogenic Leptospira interact with C1INH that maintain its regulatory capacity of interaction with C1s preventing the activation of Complement system. Although the interaction with C1INH is not crucial for pathogenic Leptospira survival, it seems to be important for the survival of attenuated and saprophytic Leptospira in normal human serum.
MeSH term(s)	Animals ; Complement Activation ; Complement C1/metabolism ; Complement C1 Inhibitor Protein/genetics ; Complement C1 Inhibitor Protein/metabolism ; Complement C4b-Binding Protein/metabolism ; Complement Factor H/metabolism ; Food Chain ; Humans ; Immune Evasion ; Leptospiraceae/immunology ; Leptospiraceae/pathogenicity ; Leptospirosis/immunology ; Vaccines, Attenuated ; Virulence ; Vitronectin/metabolism ; Zoonoses
Chemical Substances	CFH protein, human ; Complement C1 ; Complement C1 Inhibitor Protein ; Complement C4b-Binding Protein ; SERPING1 protein, human ; Vaccines, Attenuated ; Vitronectin ; Complement Factor H (80295-65-4)
Language	English
Publishing date	2017-10-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	563292-4
ISSN	1878-3279 ; 0171-2985
ISSN (online)	1878-3279
ISSN	0171-2985
DOI	10.1016/j.imbio.2017.10.027
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Article: Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt.

Moreno, Luisa Zanolli / Miraglia, Fabiana / de Oliveira, Carolina Helena / Vasconcellos, Silvio Arruda / Heinemann, Marcos Bryan / Moreno, Andrea Micke

Genome announcements

2018 Volume 6, Issue 23

Abstract: Leptospira ... ...

Abstract	Leptospira interrogans
Language	English
Publishing date	2018-06-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2704277-7
ISSN	2169-8287
ISSN	2169-8287
DOI	10.1128/genomeA.00491-18
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Article ; Online: Serological survey and DNA screening of Leptospira spp. in free-living adult tufted capuchin monkeys (Cebus apella nigritus) in a forest reserve Southeast São Paulo State, Brazil.

Girio, Raul José Silva / de Andrade-Cruvinel, Tatiana Morosini / Vasconcellos, Silvio Arruda / Repetti, Cláudia Sampaio Fonseca / Friolani, Milena / Bueno, Patrícia Cincotto Dos Santos / Felix, Marcílio / Teixeira, Daniel De Bortoli

Journal of medical primatology

2020 Volume 50, Issue 1, Page(s) 3–8

Abstract: Background: Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti-Leptospira antibodies and detection of Leptospira spp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a ... ...

Abstract	Background: Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti-Leptospira antibodies and detection of Leptospira spp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a forest reserve from Southeast São Paulo State, Brazil. Methods: Blood samples were obtained from 50 adult tufted capuchin monkeys (Cebus apella nigritus). Urine samples were obtained only from male primates. The screening for antibodies against Leptospira spp was evaluated by microscopic agglutination test (MAT). Leptospira DNA in the urine was evaluated by polymerase chain reaction (PCR) considering the target gene LipL32. Biochemical profile was evaluated by using a spectrophotometer. Results: The MAT results included 39 (78%) serum reactive animals with the proportions of 28/39 males and 11/39 females. The most frequent reactive serogroups were Icterohemorrhagiae, Canicola, and Autumnalis. All urine samples were negative for leptospiral DNA. There were no significant differences between sexes for aspartate aminotransferase (AST) and alkaline phosphatase values, but alanine aminotransferase (ALT), creatinine, glucose, and urea were significantly higher in males. Conclusions: Tufted capuchin monkeys were sera reactive against leptospirosis. Prevalence was similar for the 2 sexes. Leptospiral DNA was not detected in the urine of sera reactive primates tested by the MAT method. ALT, creatinine, glucose, and urea values were higher in male animals.
MeSH term(s)	Animals ; Antibodies, Bacterial/blood ; Brazil/epidemiology ; Cebinae ; DNA, Bacterial/urine ; Kidney/microbiology ; Kidney/pathology ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Liver/microbiology ; Liver/pathology ; Male ; Monkey Diseases/epidemiology ; Monkey Diseases/microbiology
Chemical Substances	Antibodies, Bacterial ; DNA, Bacterial
Language	English
Publishing date	2020-09-02
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	121206-0
ISSN	1600-0684 ; 0047-2565
ISSN (online)	1600-0684
ISSN	0047-2565
DOI	10.1111/jmp.12493
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Article ; Online: Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and β2 integrin.

Cosate, Maria Raquel / Siqueira, Gabriela Hase / de Souza, Gisele Oliveira / Vasconcellos, Silvio Arruda / Nascimento, Ana Lucia T O

Microbiology and immunology

2016 Volume 60, Issue 9, Page(s) 586–598

Abstract: A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and ... ...

Abstract	A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and invasion through the hosts. It has been shown that Mce of pathogenic Leptospira spp. is an RGD (Arg-Gly-Asp)-motif-dependent virulence factor, responsible for infection of cells and animals. In the present article, we decided to further study the repertoire of the Mce activities in leptospiral biological properties. We report that the recombinant Mce is a broad-spectrum ECM-binding protein, capable of interacting with laminin, cellular and plasma fibronectin and collagen IV. Dose--r-esponse interaction was observed for all the components, fulfilling ligand--receptor requirements. Mce is a PLG binding protein capable to recruit this component from NHS, generating PLA in the presence of PLG activator. Binding of Mce was also observed with the leukocyte cell receptors αLβ2 [(CD11a/CD18)-LFA-1] and αMβ2 [(CD11b/CD18)-Mac-1], suggesting the involvement of this protein in the host immune response. Indeed, virulent Leptospira L1-130 was capable of binding both integrins, whereas culture-attenuated M-20 strain only bind to αMβ2 [(CD11b/CD18)-Mac-1]. To the best of our knowledge, this is the first work to describe that Mce surface protein could mediate the attachment of Leptospira interrogans to human cell receptors αLβ2(CD11a/CD18) and αMβ2(CD11b/CD18).
MeSH term(s)	Amino Acid Sequence ; Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; CD18 Antigens/metabolism ; Enzyme Activation ; Extracellular Matrix/metabolism ; Fibrinolysin/metabolism ; Host-Pathogen Interactions ; Humans ; Leptospira interrogans/physiology ; Leptospirosis/metabolism ; Leptospirosis/microbiology ; Ligands ; Lymphocyte Function-Associated Antigen-1/metabolism ; Macrophage-1 Antigen/metabolism ; Open Reading Frames ; Plasminogen/metabolism ; Protein Binding ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
Chemical Substances	Bacterial Proteins ; CD18 Antigens ; Ligands ; Lymphocyte Function-Associated Antigen-1 ; Macrophage-1 Antigen ; Recombinant Proteins ; Plasminogen (9001-91-6) ; Fibrinolysin (EC 3.4.21.7)
Language	English
Publishing date	2016-09
Publishing country	Australia
Document type	Journal Article
ZDB-ID	224792-6
ISSN	1348-0421 ; 0385-5600
ISSN (online)	1348-0421
ISSN	0385-5600
DOI	10.1111/1348-0421.12406
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Article: ANTIBODIES AGAINST BRUCELLA ABORTUS AND LEPTOSPIRA SPP. IN CAPTIVE MAMMALS IN THE STATES OF PARá AND RIO GRANDE DO NORTE, BRAZIL.

Minervino, Antonio Humberto Hamad / Soares, Herbert Sousa / Barrêto-Júnior, Raimundo Alves / Neves, Kedson Alessandri Lobo / Morini, Adriana Caroprezo / Ortolani, Enrico Lippi / Vasconcellos, Silvio Arruda / Soares, Rodrigo Martins / Gennari, Solange Maria

Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians

2018 Volume 49, Issue 2, Page(s) 355–360

Abstract: Brazil has a large variety of wild animal species, but limited data are available on the occurrence of Brucella abortus and Leptospira spp. antibodies in these animals. Sera from 141 captive mammals belonging to 11 different species from the Northern and ...

Abstract	Brazil has a large variety of wild animal species, but limited data are available on the occurrence of Brucella abortus and Leptospira spp. antibodies in these animals. Sera from 141 captive mammals belonging to 11 different species from the Northern and Northeastern regions of Brazil were screened. Antibodies against B. abortus and Leptospira spp. (24 live serovars) were investigated using the Rose Bengal plate and microscopic agglutination tests, respectively. Associations between the age, gender, and place of captivity were analyzed using the Pearson chi-square or the Fisher exact test. None of the animals were antibody positive for B. abortus. Among the animals tested, 11 (7.8%) were seropositive for Leptospira spp. These included one red brocket deer ( Mazama americana), two tufted capuchin ( Sapajus apella), seven agoutis ( Dasyprocta aguti), and one lowland paca ( Cuniculus paca). No association was observed between sex, age, and the occurrence of Leptospira spp. antibodies ( P > 0.05). However, an association was observed according to the place of captivity ( P = 0.046). From these 11 positive animals, six (54.5%) reacted to the serovars from the Icterohaemorraghiae serogroup, which is mainly responsible for the clinical cases of human leptospirosis in Brazil. To our knowledge, this is the first report of Leptospira spp. antibodies in M. americana and C. paca.
MeSH term(s)	Animals ; Animals, Zoo ; Antibodies, Bacterial/blood ; Brazil/epidemiology ; Brucella abortus/isolation & purification ; Brucellosis/epidemiology ; Brucellosis/microbiology ; Brucellosis/veterinary ; Cebinae ; Cuniculidae ; Dasyproctidae ; Deer ; Female ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Male ; Monkey Diseases/epidemiology ; Monkey Diseases/microbiology ; Prevalence ; Rodent Diseases/epidemiology ; Rodent Diseases/microbiology ; Seroepidemiologic Studies ; Serogroup
Chemical Substances	Antibodies, Bacterial
Language	English
Publishing date	2018-06-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2174930-9
ISSN	1937-2825 ; 1042-7260
ISSN (online)	1937-2825
ISSN	1042-7260
DOI	10.1638/2015-0246.1
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Article ; Online: Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry.

Karcher, Daniel / Grenfell, Rafaella C / Moreno, Andrea Micke / Moreno, Luisa Zanolli / Vasconcellos, Silvio Arruda / Heinemann, Marcos B / Junior, Joao N de Almeida / Juliano, Luiz / Juliano, Maria A

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

2018 Volume 49, Issue 4, Page(s) 900–908

Abstract: Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight ... ...

Abstract	Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools™ software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri.
MeSH term(s)	Bacterial Typing Techniques/methods ; Brazil ; DNA, Bacterial/genetics ; Humans ; Leptospira/chemistry ; Leptospira/classification ; Leptospira/genetics ; Leptospira/isolation & purification ; Leptospirosis/microbiology ; RNA, Ribosomal, 16S/genetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Tandem Mass Spectrometry/methods
Chemical Substances	DNA, Bacterial ; RNA, Ribosomal, 16S
Language	English
Publishing date	2018-04-13
Publishing country	Brazil
Document type	Evaluation Studies ; Journal Article
ZDB-ID	2017175-4
ISSN	1678-4405 ; 1517-8382
ISSN (online)	1678-4405
ISSN	1517-8382
DOI	10.1016/j.bjm.2018.03.005
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Article: Efficacy of leptospiral commercial vaccines on the protection against an autochtonous strain recovered in Brazil

Sonada, Rafael Bazaglia / Azevedo, Sérgio Santos de / Soto, Francisco Rafael Martins / Costa, Diego Figueiredo da / Morais, Zenaide Maria de / Souza, Gisele Oliveira de / Gonçales, Amane Paldês / Miraglia, Fabiana / Vasconcellos, Sílvio Arruda

Sociedade Brasileira de Microbiologia Brazilian Journal of Microbiology. 2017,

2017

Abstract: In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five ... ...

Abstract	In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in Brazil for the immunization of swine or bovines failed the test of the efficacy to protect the vaccinated animals following challenge with an autochthonous Leptospira strain, Canicola serovar. The two vaccines provided protection against the renal carrier state in the surviving animals. The criteria used to produce leptospirosis bacterins sold commercially in Brazil must be reviewed. The industry should support researches on leptospiral vaccinology to improve the quality of the present vaccines and discover new immunogenic strains, because it is known that vaccination is one of the most important tools to increase the reproduction rates in livestock.
Keywords	Leptospira ; carrier state ; cattle ; industry ; leptospirosis ; microbiology ; polymerase chain reaction ; reproduction ; serotypes ; swine ; vaccination ; vaccine development ; vaccines ; Brazil
Language	English
Publishing place	Elsevier Editora Ltda.
Document type	Article
Note	Pre-press version
ZDB-ID	2042078-X
ISSN	1517-8382
ISSN	1517-8382
DOI	10.1016/j.bjm.2017.06.008
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Article ; Online: Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis.

do Rosário, Tatiana Reis / Dib, Cristina Corsi / Roxo, Eliana / Pinheiro, Sônia Regina / Vasconcellos, Silvio Arruda / Benites, Nilson Roberti

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

2014 Volume 45, Issue 1, Page(s) 225–230

Abstract: The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of ... ...

Abstract	The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12(nd) and 25(th) day of culture).
MeSH term(s)	Abattoirs ; Animals ; Bacteriological Techniques/methods ; Cattle ; Meat/microbiology ; Microscopy ; Molecular Diagnostic Techniques/methods ; Mycobacterium bovis/isolation & purification ; Polymerase Chain Reaction/methods ; Staining and Labeling ; Time Factors ; Tuberculosis, Bovine/diagnosis
Language	English
Publishing date	2014-05-02
Publishing country	Brazil
Document type	Comparative Study ; Evaluation Study ; Journal Article
ZDB-ID	2017175-4
ISSN	1678-4405 ; 1517-8382
ISSN (online)	1678-4405
ISSN	1517-8382
DOI	10.1590/S1517-83822014005000038
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Article ; Online: Cross-protection between experimental anti-leptospirosis bacterins.

Dib, Cristina Corsi / Gonçales, Amane Paldês / de Morais, Zenaide Maria / de Souza, Gisele Oliveira / Miraglia, Fabiana / Abreu, Patricia Antonia Estima / Vasconcellos, Silvio Arruda

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

2014 Volume 45, Issue 3, Page(s) 1083–1088

Abstract: We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and ... ...

Abstract	We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil.
MeSH term(s)	Adjuvants, Immunologic/administration & dosage ; Aluminum Hydroxide/administration & dosage ; Animals ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/immunology ; Carrier State/microbiology ; Carrier State/prevention & control ; Cricetinae ; Cross Protection ; Kidney/microbiology ; Leptospira/isolation & purification ; Leptospirosis/immunology ; Leptospirosis/prevention & control ; Treatment Outcome
Chemical Substances	Adjuvants, Immunologic ; Bacterial Vaccines ; Aluminum Hydroxide (5QB0T2IUN0)
Language	English
Publishing date	2014-10-09
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2017175-4
ISSN	1678-4405 ; 1517-8382
ISSN (online)	1678-4405
ISSN	1517-8382
DOI	10.1590/s1517-83822014000300042
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