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  1. Article ; Online: Bivalent omicron (BA.1) booster vaccination against SARS-CoV-2.

    Vasin, Andrey V / Stukova, Marina A

    The Lancet. Infectious diseases

    2023  Volume 23, Issue 8, Page(s) 880–881

    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19/prevention & control ; Immunization, Secondary ; Vaccination
    Language English
    Publishing date 2023-04-21
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 2061641-7
    ISSN 1474-4457 ; 1473-3099
    ISSN (online) 1474-4457
    ISSN 1473-3099
    DOI 10.1016/S1473-3099(23)00189-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Exosomes as Natural Nanocarriers for RNA-Based Therapy and Prophylaxis.

    Gorshkov, Andrey / Purvinsh, Lada / Brodskaia, Alexandra / Vasin, Andrey

    Nanomaterials (Basel, Switzerland)

    2022  Volume 12, Issue 3

    Abstract: Exosomes are natural nanocontainers actively secreted by the body's cells and transmitting molecular signals of various types to recipient cells. Cellular mechanisms of exosomes' biogenesis involve specific sorting of RNA for incorporation into them. As ... ...

    Abstract Exosomes are natural nanocontainers actively secreted by the body's cells and transmitting molecular signals of various types to recipient cells. Cellular mechanisms of exosomes' biogenesis involve specific sorting of RNA for incorporation into them. As a result, the molecular composition of exosomes is closely related to the donor cell's functional state, and this makes exosomes an important diagnostic and prognostic marker in a number of diseases (primarily oncological). The ability of exosomes to transport biologically active molecules and to protect the cargo from degradation makes them nearly ideal candidates as delivery carriers of RNA in therapeutic or prophylactic regimes. Potential of exosomal surface functionalization enables improved targeting to specific organs, tissues and cells. However, the development of an effective technology for RNA's loading into exosomes cannot be considered resolved. This review is focused on experimental data on the use of exosomes as vehicles for the delivery of therapeutic and prophylactic RNAs. We briefly consider the biogenesis and functions of exosomes, focusing on those biological properties that make them formidable candidates in the race to develop effective delivery carriers. Furthermore, we describe various techniques of cargo loading into exosomes. Prospects of exosomes application as therapeutic delivery system for siRNAs, miRNAs, and long RNAs are considered.
    Language English
    Publishing date 2022-02-02
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano12030524
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Extracellular Vesicles in Viral Pathogenesis: A Case of Dr. Jekyll and Mr. Hyde.

    Purvinsh, Lada / Gorshkov, Andrey / Brodskaia, Aleksandra / Vasin, Andrey

    Life (Basel, Switzerland)

    2021  Volume 11, Issue 1

    Abstract: Secretion of extracellular vesicles (EVs) is a fundamental property of living cells. EVs are known to transfer biological signals between cells and thus regulate the functional state of recipient cells. Such vesicles mediate the intercellular transport ... ...

    Abstract Secretion of extracellular vesicles (EVs) is a fundamental property of living cells. EVs are known to transfer biological signals between cells and thus regulate the functional state of recipient cells. Such vesicles mediate the intercellular transport of many biologically active molecules (proteins, nucleic acids, specific lipids) and participate in regulation of key physiological processes. In addition, EVs are involved in the pathogenesis of multiple diseases: infectious, neurodegenerative, and oncological. The current EV classification into microvesicles, apoptotic bodies, and exosomes is based on their size, pathways of cellular biogenesis, and molecular composition. This review is focused on analysis of the role of EVs (mainly exosomes) in the pathogenesis of viral infection. We briefly characterize the biogenesis and molecular composition of various EV types. Then, we consider EV-mediated pro- and anti-viral mechanisms. EV secretion by infected cells can be an important factor of virus spread in target cell populations, or a protective factor limiting viral invasion. The data discussed in this review, on the effect of EV secretion by infected cells on processes in neighboring cells and on immune cells, are of high significance in the search for new therapeutic approaches and for design of new generations of vaccines.
    Language English
    Publishing date 2021-01-13
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life11010045
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Influenza B: Prospects for the Development of Cross-Protective Vaccines.

    Tsybalova, Liudmila M / Stepanova, Liudmila A / Ramsay, Edward S / Vasin, Andrey V

    Viruses

    2022  Volume 14, Issue 6

    Abstract: In this review, we analyze the epidemiological and ecological features of influenza B, one of the most common and severe respiratory infections. The review presents various strategies for cross-protective influenza B vaccine development, including ... ...

    Abstract In this review, we analyze the epidemiological and ecological features of influenza B, one of the most common and severe respiratory infections. The review presents various strategies for cross-protective influenza B vaccine development, including recombinant viruses, virus-like particles, and recombinant proteins. We provide an overview of viral proteins as cross-protective vaccine targets, along with other updated broadly protective vaccine strategies. The importance of developing such vaccines lies not only in influenza B prevention, but also in the very attractive prospect of eradicating the influenza B virus in the human population.
    MeSH term(s) Antibodies, Viral ; Cross Protection ; Hemagglutinin Glycoproteins, Influenza Virus ; Humans ; Influenza Vaccines ; Influenza, Human/epidemiology ; Influenza, Human/prevention & control ; Orthomyxoviridae Infections
    Chemical Substances Antibodies, Viral ; Hemagglutinin Glycoproteins, Influenza Virus ; Influenza Vaccines
    Language English
    Publishing date 2022-06-17
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061323
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Uncertainty Estimation for Quantitative Agarose Gel Electrophoresis of Nucleic Acids.

    Semenov, Konstantin / Taraskin, Aleksandr / Yurchenko, Alexandra / Baranovskaya, Irina / Purvinsh, Lada / Gyulikhandanova, Natalia / Vasin, Andrey

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 4

    Abstract: This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory ... ...

    Abstract This paper considers the evaluation of uncertainty of quantitative gel electrophoresis. To date, such uncertainty estimation presented in the literature are based on the multiple measurements performed for assessing the intra- and interlaboratory reproducibility using standard samples. This paper shows how to estimate the uncertainty in cases where we cannot study scattering components of the results. The first point is dedicated to a case where we have standard samples (the direct expressions are shown). The second point considers the situation when standard samples are absent (the algorithm for estimating the lower bound for uncertainty is discussed). The role of the data processing algorithm is demonstrated.
    MeSH term(s) Nucleic Acids ; Reproducibility of Results ; Uncertainty ; Electrophoresis, Agar Gel ; Algorithms
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2023-02-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23041999
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  6. Article: Influenza B: Prospects for the Development of Cross-Protective Vaccines

    Tsybalova, Liudmila M. / Stepanova, Liudmila A. / Ramsay, Edward S. / Vasin, Andrey V.

    Viruses. 2022 June 17, v. 14, no. 6

    2022  

    Abstract: In this review, we analyze the epidemiological and ecological features of influenza B, one of the most common and severe respiratory infections. The review presents various strategies for cross-protective influenza B vaccine development, including ... ...

    Abstract In this review, we analyze the epidemiological and ecological features of influenza B, one of the most common and severe respiratory infections. The review presents various strategies for cross-protective influenza B vaccine development, including recombinant viruses, virus-like particles, and recombinant proteins. We provide an overview of viral proteins as cross-protective vaccine targets, along with other updated broadly protective vaccine strategies. The importance of developing such vaccines lies not only in influenza B prevention, but also in the very attractive prospect of eradicating the influenza B virus in the human population.
    Keywords Influenza B virus ; human population ; influenza ; vaccine development ; vaccines
    Language English
    Dates of publication 2022-0617
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061323
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.

    Nikolaev, Dmitrii M / Mironov, Vladimir N / Shtyrov, Andrey A / Kvashnin, Iaroslav D / Mereshchenko, Andrey S / Vasin, Andrey V / Panov, Maxim S / Ryazantsev, Mikhail N

    International journal of molecular sciences

    2023  Volume 24, Issue 3

    Abstract: Membrane potential is a fundamental property of biological cells. Changes in membrane potential characterize a vast number of vital biological processes, such as the activity of neurons and cardiomyocytes, tumorogenesis, cell-cycle progression, etc. A ... ...

    Abstract Membrane potential is a fundamental property of biological cells. Changes in membrane potential characterize a vast number of vital biological processes, such as the activity of neurons and cardiomyocytes, tumorogenesis, cell-cycle progression, etc. A common strategy to record membrane potential changes that occur in the process of interest is to utilize organic dyes or genetically-encoded voltage indicators with voltage-dependent fluorescence. Sensors are introduced into target cells, and alterations of fluorescence intensity are recorded with optical methods. Techniques that allow recording relative changes of membrane potential and do not take into account fluorescence alterations due to factors other than membrane voltage are already widely used in modern biological and biomedical studies. Such techniques have been reviewed previously in many works. However, in order to investigate a number of processes, especially long-term processes, the measured signal must be corrected to exclude the contribution from voltage-independent factors or even absolute values of cell membrane potential have to be evaluated. Techniques that enable such measurements are the subject of this review.
    MeSH term(s) Membrane Potentials/physiology ; Cell Membrane/metabolism ; Fluorescent Dyes/metabolism ; Neurons/metabolism ; Optical Imaging
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2023-01-26
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24032435
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  8. Article ; Online: Fluorescence of the Retinal Chromophore in Microbial and Animal Rhodopsins.

    Nikolaev, Dmitrii M / Shtyrov, Andrey A / Vyazmin, Sergey Yu / Vasin, Andrey V / Panov, Maxim S / Ryazantsev, Mikhail N

    International journal of molecular sciences

    2023  Volume 24, Issue 24

    Abstract: Fluorescence of the vast majority of natural opsin-based photoactive proteins is extremely low, in accordance with their functions that depend on efficient transduction of absorbed light energy. However, several recently proposed classes of engineered ... ...

    Abstract Fluorescence of the vast majority of natural opsin-based photoactive proteins is extremely low, in accordance with their functions that depend on efficient transduction of absorbed light energy. However, several recently proposed classes of engineered rhodopsins with enhanced fluorescence, along with the discovery of a new natural highly fluorescent rhodopsin, NeoR, opened a way to exploit these transmembrane proteins as fluorescent sensors and draw more attention to studies on this untypical rhodopsin property. Here, we review the available data on the fluorescence of the retinal chromophore in microbial and animal rhodopsins and their photocycle intermediates, as well as different isomers of the protonated retinal Schiff base in various solvents and the gas phase.
    MeSH term(s) Animals ; Rhodopsin/metabolism ; Fluorescence ; Retina/metabolism
    Chemical Substances Rhodopsin (9009-81-8)
    Language English
    Publishing date 2023-12-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms242417269
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  9. Article: Kinetics of interferon-λ and receptor expression in response to in vitro respiratory viral infection.

    Lozhkov, Alexey A / Yolshin, Nikita D / Baranovskaya, Irina L / Plotnikova, Marina A / Sergeeva, Maria V / Gyulikhandanova, Natalia E / Klotchenko, Sergey A / Vasin, Andrey V

    Acta virologica

    2023  Volume 67, Issue 1, Page(s) 99–108

    Abstract: The major protective immune response against viruses is the production of type I and III interferons (IFNs). IFNs induce the expression of hundreds of IFN-stimulated genes (ISGs) that block viral replication and further viral spread. In this report, we ... ...

    Abstract The major protective immune response against viruses is the production of type I and III interferons (IFNs). IFNs induce the expression of hundreds of IFN-stimulated genes (ISGs) that block viral replication and further viral spread. In this report, we analyzed the expression of IFNs and some ISGs (MxA, PKR, OAS-1, IFIT-1, RIG-1, MDA5, SOCS-1) in alveolar epithelial cells (A549) in response to infection with influenza A viruses (A/California/07/09 (H1N1pdm); A/Texas/50/12 (H3N2)); influenza B virus (B/Phuket/3073/13); adenovirus type 5 and 6; or respiratory syncytial virus (strain A2). Influenza B virus had the ability to most rapidly induce IFNs and ISGs as well as to stimulate excessive IFN-α, IFN-β and IFN-λ secretion. It seems curious that IAV H1N1pdm did not induce IFN-λ secretion, but enhanced type I IFN and interleukin (IL)-6 production. We emphasized the importance of the negative regulation of virus-triggered signaling and cellular IFN response. We showed a decrease in IFNLR1 mRNA in the case of IBV infection. The attenuation of SOCS-1 expression in IAV H1N1pdm can be considered as the inability of the system to restore the immune status. Presumably, the lack of negative feedback loop regulation of proinflammatory immune response may be a factor contributing to the particular pathogenicity of several strains of influenza. Keywords: lambda interferons; MxA; influenza; respiratory syncytial virus; A549 cells.
    MeSH term(s) Humans ; Interferon Lambda ; Influenza, Human/genetics ; Influenza A Virus, H3N2 Subtype ; Interferons/genetics ; Interferons/pharmacology ; Interferon-alpha/genetics ; Gene Expression
    Chemical Substances Interferon Lambda ; Interferons (9008-11-1) ; Interferon-alpha
    Language English
    Publishing date 2023-03-23
    Publishing country Slovakia
    Document type Journal Article
    ZDB-ID 210452-0
    ISSN 1336-2305 ; 0001-723X
    ISSN (online) 1336-2305
    ISSN 0001-723X
    DOI 10.4149/av_2023_110
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  10. Article ; Online: Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro.

    Vysochinskaya, Vera / Zabrodskaya, Yana / Dovbysh, Olesya / Emelyanov, Anton / Klimenko, Vladimir / Knyazev, Nikolay / Terterov, Ivan / Egorova, Marya / Bogdanov, Alexey / Maslov, Michael / Vasin, Andrey / Dubina, Michael

    Biochimie

    2024  Volume 221, Page(s) 1–12

    Abstract: Gene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional ... ...

    Abstract Gene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional chronic myeloid leukemia (CML) tyrosine kinase inhibitor (TKIs) therapies, given the prevalence of clinical TKI resistance. The main challenge for such approaches remains the development of the effective delivery system for siRNA tailored to the specific disease model. The purpose of this study was to examine and compare the efficiency of endosomolytic cell penetrating peptide (CPP) EB1 and PEG
    Language English
    Publishing date 2024-01-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 120345-9
    ISSN 1638-6183 ; 0300-9084
    ISSN (online) 1638-6183
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2024.01.006
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