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  1. AU="Vasisth, Rashi"
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  1. Article ; Online: ASIP gene polymorphism associated with black coat and skin color in Murrah buffalo

    Kumari, Namita / Vasisth, Rashi / Gurao, Ankita / Mukesh, Manishi / Vohra, Vikas / Kumar, Sanjay / Kataria, Ranjit Singh

    Environmental and Molecular Mutagenesis. 2023 June, v. 64, no. 5, p. 309-314

    2023  , Page(s) 309–314

    Abstract: The melanogenesis pathway regulates pigmentation through the synergic action of various genes. We are interested in analyzing the genetic variations in the ASIP which determine eumelanin production in the dermis layer. In the present study, the ASIP gene ...

    Abstract The melanogenesis pathway regulates pigmentation through the synergic action of various genes. We are interested in analyzing the genetic variations in the ASIP which determine eumelanin production in the dermis layer. In the present study, the ASIP gene was characterized in buffalo and 268 genetically unrelated buffaloes belonging to 10 different populations were genotyped for the non‐synonymous SNP (c.292C>T) identified in the exon 3 region of the gene using Tetra‐ARMS‐PCR. The TT genotype occurred at a higher rate in Murrah, followed by Nili Ravi, Tripura, and Paralakhemundi (42.63%, 19.30%, 3.45%, and 3.33%). These results convey the association of the black coat color of Murrah with the ASIP gene TT genotype and the lighter shades of black coat (brown and grayish‐black) color phenotype in other breeds with the CC genotype.
    Keywords buffaloes ; color ; dermis ; eumelanin ; exons ; genetic polymorphism ; genotype ; genotyping ; melanogenesis ; mutagenesis ; phenotype ; pigmentation ; synergism ; India
    Language English
    Dates of publication 2023-06
    Size p. 309-314
    Publishing place John Wiley & Sons, Inc.
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 639145-x
    ISSN 1098-2280 ; 0893-6692
    ISSN (online) 1098-2280
    ISSN 0893-6692
    DOI 10.1002/em.22554
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2404: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: ASIP gene polymorphism associated with black coat and skin color in Murrah buffalo.

    Kumari, Namita / Vasisth, Rashi / Gurao, Ankita / Mukesh, Manishi / Vohra, Vikas / Kumar, Sanjay / Kataria, Ranjit Singh

    Environmental and molecular mutagenesis

    2023  Volume 64, Issue 5, Page(s) 309–314

    Abstract: The melanogenesis pathway regulates pigmentation through the synergic action of various genes. We are interested in analyzing the genetic variations in the ASIP which determine eumelanin production in the dermis layer. In the present study, the ASIP gene ...

    Abstract The melanogenesis pathway regulates pigmentation through the synergic action of various genes. We are interested in analyzing the genetic variations in the ASIP which determine eumelanin production in the dermis layer. In the present study, the ASIP gene was characterized in buffalo and 268 genetically unrelated buffaloes belonging to 10 different populations were genotyped for the non-synonymous SNP (c.292C>T) identified in the exon 3 region of the gene using Tetra-ARMS-PCR. The TT genotype occurred at a higher rate in Murrah, followed by Nili Ravi, Tripura, and Paralakhemundi (42.63%, 19.30%, 3.45%, and 3.33%). These results convey the association of the black coat color of Murrah with the ASIP gene TT genotype and the lighter shades of black coat (brown and grayish-black) color phenotype in other breeds with the CC genotype.
    MeSH term(s) Animals ; Skin Pigmentation/genetics ; Buffaloes/genetics ; Polymorphism, Genetic ; Genotype ; Phenotype
    Language English
    Publishing date 2023-06-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639145-x
    ISSN 1098-2280 ; 0893-6692
    ISSN (online) 1098-2280
    ISSN 0893-6692
    DOI 10.1002/em.22554
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2404: Show issues Location:
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  3. Article ; Online: Identification of differential methylome signatures of white pigmented skin patches in Nili Ravi buffalo of India

    Gurao, Ankita / Vasisth, Rashi / Singh, Ravinder / Dige, Mahesh S. / Vohra, Vikas / Mukesh, Manishi / Kumar, Sanjay / Kataria, Ranjit S.

    Environmental and Molecular Mutagenesis. 2022 Dec., v. 63, no. 8-9, p. 408-417

    2022  , Page(s) 408–417

    Abstract: The DNA methylation events mark a major epigenetic change in the genome, reflecting non‐genetic disease developments and varied phenotypes. The water buffalo is a dairy production animal with wide agro‐climatic distribution in India. Breed‐wise the coat ... ...

    Abstract The DNA methylation events mark a major epigenetic change in the genome, reflecting non‐genetic disease developments and varied phenotypes. The water buffalo is a dairy production animal with wide agro‐climatic distribution in India. Breed‐wise the coat color of water buffalo varies from ash‐gray to jet black. A typical pigmentation pattern is found in one of the breeds of North India, Nili Ravi, with variedly distributed white patches. The DNA methylation pattern could potentially reveal the epigenetic factors responsible for the pigmentation patterns. To address this question, the DNA isolated from the skin tissues of Nili Ravi with varied white pigmentation and black Murrah buffaloes was subjected to reduced representation bisulfite sequencing. DNA methylation analysis revealed, 68.44%, 63.39%, and 47.94% of the promoter regions were hypermethylated in Nili Ravi over‐white versus Murrah, Nili Ravi under‐white versus Murrah, and Nili Ravi under‐white versus Nili Ravi over‐white, respectively. Major genes identified to be differentially methylated among over‐white and under‐white skin tissues in Nili Ravi included TBX2, SNAI2, HERC2, and CITED1. Overall the results have indicated differential methylation patterns to be potentially involved in hyper or hypopigmentation in Nili Ravi and Murrah buffaloes.
    Keywords DNA ; DNA methylation ; bisulfites ; buffaloes ; color ; epigenetics ; genome ; milk production ; mutagenesis ; pigmentation ; India
    Language English
    Dates of publication 2022-12
    Size p. 408-417
    Publishing place John Wiley & Sons, Inc.
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 639145-x
    ISSN 1098-2280 ; 0893-6692
    ISSN (online) 1098-2280
    ISSN 0893-6692
    DOI 10.1002/em.22511
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2404: Show issues Location:
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  4. Article ; Online: Genome-wide 5'-C-phosphate-G-3' methylation patterns reveal the effect of heat stress on the altered semen quality in Bubalus bubalis.

    Kumar, Gautam / Gurao, Ankita / Vasisth, Rashi / Chitkara, Meenakshi / Singh, Ravinder / Ranganatha Sriranga, Karpenahalli / Shivanand Dige, Mahesh / Mukesh, Manishi / Singh, Pawan / Singh Kataria, Ranjit

    Gene

    2024  Volume 906, Page(s) 148233

    Abstract: Semen production and quality are closely correlated with different environmental factors in bovines, particularly for the buffalo (Bubalus bubalis) bulls reared under tropical and sub-tropical conditions. Factors including DNA methylation patterns, an ... ...

    Abstract Semen production and quality are closely correlated with different environmental factors in bovines, particularly for the buffalo (Bubalus bubalis) bulls reared under tropical and sub-tropical conditions. Factors including DNA methylation patterns, an intricate process in sperm cells, have an impact on the production of quality semen in buffalo bulls under abiotic stress conditions. The present study was conducted to identify DNA methylome signatures for semen quality in Murrah buffalo bulls, acclaimed as a major dairy breed globally, under summer heat stress. Based on semen quality parameters that significantly varied between the two groups over the seasons, the breeding bulls were classified into seasonally affected (SA = 6) and seasonally non-affected (SNA = 6) categories. DNA was isolated from purified sperm cells and sequenced using the RRBS (Reduced Representation Bisulfite Sequencing) technique for genome-wide methylome data generation. During the hot summer months, the physiological parameters such as scrotal surface temperature, rectal temperature, and respiration rate for both the SA and SNA bulls were significantly higher in the afternoon than in the morning. Whereas, the global CpG% of SA bulls was positively correlated with the afternoon's scrotal surface and rectal temperature. The RRBS results conveyed differentially methylated cytosines in the promoter region of the genes encoding the channels responsible for Ca
    MeSH term(s) Animals ; Male ; Cattle/genetics ; Semen Analysis ; Semen/physiology ; Buffaloes/genetics ; Phosphates ; Spermatozoa ; DNA Methylation ; Heat-Shock Response/genetics ; Sperm Motility
    Chemical Substances Phosphates
    Language English
    Publishing date 2024-02-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/j.gene.2024.148233
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1357: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Selection of reference genes for normalizing gene expression data across seasons in spermatozoa of water buffalo (Bubalus bubalis).

    Vasisth, Rashi / Gurao, Ankita / Chitkara, Meenakshi / Kumar, Gautam / Sriranga, Karpenahalli Ranganatha / Mukesh, Manishi / Dige, Mahesh Shivanand / Singh, Pawan / Aggarwal, Rajeev Anand Kumar / Kataria, Ranjit Singh

    International journal of biometeorology

    2024  

    Abstract: Selection of the most stably expressed reference genes is key to monitoring accurate target gene expression across any tissue or cell type. The mRNA in spermatozoa stores valuable information related to changes in spermatogenesis due to variations in ... ...

    Abstract Selection of the most stably expressed reference genes is key to monitoring accurate target gene expression across any tissue or cell type. The mRNA in spermatozoa stores valuable information related to changes in spermatogenesis due to variations in environmental conditions, especially during heat stress, which affects various sperm functions. Semen quality in buffalo bulls is significantly influenced by the seasons. In the study, a panel of nine genes was evaluated to identify the most stably expressed internal control gene (ICG) for the normalization of real-time gene expression data generated across various seasons for Murrah buffalo bulls' spermatozoa. Sperm cells were purified from the semen samples collected during different seasons, with temperature-humidity index (THI) ranging from 80.80 ± 1.47 (hot summer) to 55.88 ± 1.98 (winter), using the BoviPure™ gradient purification method. The RNA isolated from the purified spermatozoa fraction was quality checked prior to reverse transcription and subjected to qPCR (quantitative real-time PCR) based expression analysis. An automated 'endoGene' pipeline was employed to apply the geNorm, NormFinder, and BestKeeper algorithms for data analysis. The result indicated that GAPDH and PP1A were the most stably expressed among the gene panel, whereas ATPSF1 and ACTB were the two least stable expressed reference genes. Further, the most suitable ICGs identified were validated by normalization of real time expression data of heat stress and sperm quality genes, HSFY2 and AKAP4, respectively. The genes identified would help in generating the most reliable results for the expression profiling of the genes dictating sperm quality and heat stress cope-up mechanism in buffalo spermatozoa, collected during different seasons.
    Language English
    Publishing date 2024-04-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 280324-0
    ISSN 1432-1254 ; 0020-7128
    ISSN (online) 1432-1254
    ISSN 0020-7128
    DOI 10.1007/s00484-024-02675-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 998: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Identification of differential methylome signatures of white pigmented skin patches in Nili Ravi buffalo of India.

    Gurao, Ankita / Vasisth, Rashi / Singh, Ravinder / Dige, Mahesh S / Vohra, Vikas / Mukesh, Manishi / Kumar, Sanjay / Kataria, Ranjit S

    Environmental and molecular mutagenesis

    2022  

    Abstract: The DNA methylation events mark a major epigenetic change in the genome, reflecting non-genetic disease developments and varied phenotypes. The water buffalo is a dairy production animal with wide agro-climatic distribution in India. Breed-wise the coat ... ...

    Abstract The DNA methylation events mark a major epigenetic change in the genome, reflecting non-genetic disease developments and varied phenotypes. The water buffalo is a dairy production animal with wide agro-climatic distribution in India. Breed-wise the coat color of water buffalo varies from ash-gray to jet black. A typical pigmentation pattern is found in one of the breeds of North India, Nili Ravi, with variedly distributed white patches. The DNA methylation pattern could potentially reveal the epigenetic factors responsible for the pigmentation patterns. To address this question, the DNA isolated from the skin tissues of Nili Ravi with varied white pigmentation and black Murrah buffaloes was subjected to reduced representation bisulfite sequencing. DNA methylation analysis revealed, 68.44%, 63.39%, and 47.94% of the promoter regions were hypermethylated in Nili Ravi over-white versus Murrah, Nili Ravi under-white versus Murrah, and Nili Ravi under-white versus Nili Ravi over-white, respectively. Major genes identified to be differentially methylated among over-white and under-white skin tissues in Nili Ravi included TBX2, SNAI2, HERC2, and CITED1. Overall the results have indicated differential methylation patterns to be potentially involved in hyper or hypopigmentation in Nili Ravi and Murrah buffaloes.
    Language English
    Publishing date 2022-10-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639145-x
    ISSN 1098-2280 ; 0893-6692
    ISSN (online) 1098-2280
    ISSN 0893-6692
    DOI 10.1002/em.22511
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2404: Show issues Location:
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  7. Article ; Online: Development and validation of most efficient RNA isolation method from buffalo bull spermatozoa.

    Vasisth, Rashi / Gurao, Ankita / Kumari, Namita / Kumar, Gautam / Kumar, Anurag / Sriranga, Karpenahalli Ranganatha / Dige, Mahesh Shivanand / Mukesh, Manishi / Aggarwal, Rajeev Anand Kumar / Singh, Pawan / Kataria, Ranjit Singh

    Molecular biology reports

    2023  Volume 50, Issue 8, Page(s) 6717–6727

    Abstract: Background: Being highly fragmented and low in concentration, isolation of good quality RNA from sperm cells is a big challenge. Attempts have been made to evaluate various sperm RNA isolation methods from purified buffalo bull sperm cells.: Methods: ...

    Abstract Background: Being highly fragmented and low in concentration, isolation of good quality RNA from sperm cells is a big challenge. Attempts have been made to evaluate various sperm RNA isolation methods from purified buffalo bull sperm cells.
    Methods: Both, non-membrane and membrane-based methods have been evaluated for isolating RNA from Murrah buffalo sperms and compared for their respective efficacies. The traditional TRIzol, TRIzol-heat lysed (H-TRIzol) and cocktail of TCEP-RLT lysis buffer (Qiagen RNeasy mini kit)-TRIzol (C-TRIzol) based isopropanol isolation methods have been evaluated.
    Results: H-TRIzol yielded best results among conventional methods. The combined T-RLT RNA isolation protocol yielded best quality and quantity compared to other membrane-based methods, due to high lytic property of cocktail of lysis reagents, necessary for complete breakdown of sperm membrane and RNA binding membrane for RNA isolation. Combined lysis performed by treatment with RLT-T and T-RLT differing in order of reagents used were also evaluated. T-RLT combination giving better results compared to RLT-T due to high gDNA contamination and membrane clogging in later protocol steps.
    Conclusion: Overall, in terms of total RNA quantity and quality per million spermatozoa, the heat-lysed TRIzol method (H-TRIzol) performs best among RNA separation techniques employed and is also quite easy to perform. This comparative evaluation of sperm RNA isolation protocols can be useful in deciding the best protocol for isolation of good quality and high concentration sperm RNA from buffalo semen, for transcriptome and other downstream studies.
    MeSH term(s) Animals ; Male ; RNA/metabolism ; Buffaloes/genetics ; Buffaloes/metabolism ; Semen/metabolism ; Spermatozoa/metabolism ; Semen Preservation/methods ; Cryopreservation/methods
    Chemical Substances trizol ; RNA (63231-63-0)
    Language English
    Publishing date 2023-06-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-023-08593-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1140: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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