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Article ; Online: ViphyllinTM, a Standardized Black Pepper Seed Extract Exerts Antinociceptive Effects in Murine Pain Models via Activation of Cannabinoid Receptor CB2, Peroxisome Proliferator-Activated Receptor-Alpha and TRPV1 Ion Channels

Venkatakrishna K / Sundeep K / Sudeep HV / Gouthamchandra K / Shyamprasad K

Journal of Pain Research, Vol Volume 15, Pp 355-

2022 Volume 366

Abstract: Karempudi Venkatakrishna, Kuppam Sundeep, Heggar Venkataramana Sudeep, Kuluvar Gouthamchandra, Kodimule Shyamprasad R&D Center for Excellence, Vidya Herbs Pvt Ltd, Bangalore, 560 105, Karnataka, IndiaCorrespondence: Heggar Venkataramana Sudeep, Tel +91 ... ...

Abstract	Karempudi Venkatakrishna, Kuppam Sundeep, Heggar Venkataramana Sudeep, Kuluvar Gouthamchandra, Kodimule Shyamprasad R&D Center for Excellence, Vidya Herbs Pvt Ltd, Bangalore, 560 105, Karnataka, IndiaCorrespondence: Heggar Venkataramana Sudeep, Tel +91 80-42094158, Email research@vidyaherbs.comPurpose: Plant-based natural products as anti-nociceptors have enormous potential as safer alternatives to conventional opiates and NSAIDS. Piper nigrum (black pepper) is one of the major culinary spices with medicinal attributes.Methods: In the present study, the antinociceptive activity of a standardized black pepper seed extract (Viphyllin) containing not less than 30% β-caryophyllene (BCP) was evaluated using pain models in mice, namely acetic acid-induced writhing test, formalin-induced paw licking test, hot plate test and tail flick test. Further, the antagonists SR141716A (0.1 mg/kg i.p.), AM630 (5 mg/kg i.p.), capsazepine (0.1 mg/kg body weight i.p.), and GW6471 (1 mg/kg i.p.) were used to evaluate the involvement of cannabinoid receptors CB1 and CB2, TRPV1 ion channel and PPARα receptor, respectively. Molecular docking (AutoDock 4.2) was used to study the interaction of BCP with the agonist-binding sites of the selected pain receptors.Results: Viphyllin at 10 mg, 25 mg and 50 mg/kg (i.p.) significantly inhibited the writhings in mice as compared to untreated control group (p < 0.001). Further, Viphyllin at 50 mg/kg showed strong antinociceptive effect in formalin-induced paw licking test (p < 0.05). Pretreatment of mice with AM630 significantly reversed the antinociceptive activity of Viphyllin in both early and late phases of formalin test (p < 0.05). Administration of Viphyllin markedly increased the latency time of mice in hot plate test (p < 0.001). Further, Viphyllin markedly increased the latency time of tail flick compared to control group from 30 min to 90 min after treatment. AM630, Capsazepine, and GW6471 abolished the analgesic effect of Viphyllin. These findings clearly suggest the ...
Keywords	piper nigrum ; β-caryophyllene ; pain ; nociceptive receptors ; Medicine (General) ; R5-920
Subject code	616
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	Dove Medical Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Combined Posterior and Convergent Elbow Dislocation in Adult: A Rare Case Report.

Mane, Akash Nagnath / Sourav, Neel Kamal / Panchnadikar, Vijay / Bhandari, Abhijit / Venkatakrishna, Karthic

Journal of orthopaedic case reports

2023 Volume 13, Issue 5, Page(s) 49–54

Abstract: Introduction: Elbow dislocations are commonly encountered by orthopedic surgeons all over. Management is straightforward and the result is generally satisfactory. However, in a small subset, associated injuries like convergent or divergent dislocations ... ...

Abstract	Introduction: Elbow dislocations are commonly encountered by orthopedic surgeons all over. Management is straightforward and the result is generally satisfactory. However, in a small subset, associated injuries like convergent or divergent dislocations at superior radioulnar joints are present. These associated injuries are often missed initially leading to their delayed treatment and suboptimal elbow function. We report one such rare case of Convergent dislocation at the elbow. Although reported from other parts of the world till date, there has been none reported from India. It is extremely rare in adults. Twenty-three cases in children and only two in adults stand reported so far in world literature. Thus, our reporting it could be the first report of convergent dislocation at the elbow from India. To the best of our knowledge, it is only the third reported adult case in world literature. Case report: We describe a 68-year-old male visiting a local hospital with a history of fall from a two-wheeler. An initial radiograph revealed an obvious posterior dislocation of the elbow. Closed reduction was performed, and posterior above elbow slab was given. Patient presented to us 10 days later with persistent pain. Evaluation with radiographs confirmed reduction of ulno-humeral joint but translocation of the radius and ulna which is combined posterior and convergent elbow dislocation. Conclusion: Combined posterior and convergent elbow dislocation is a rare scenario. The rarity of these associated injuries is the reason for lack of awareness. We suggest careful scrutiny of pre-reduction anteroposterior radiographic view or 3D CT in suspected cases as well as X-ray/Carm anteroposterior view in extended position of elbow on the operation table immediately after reduction before slab application in every case of elbow dislocation. Significant difficulty was encountered in open reduction in our case. This could be attributed to buttonholing of the radial head through insertion of brachialis fibers. The reporting of this surgical difficulty is important because it will help the surgeon know it beforehand and the awareness would make the surgeon take appropriate measures well in time.
Language	English
Publishing date	2023-05-31
Publishing country	India
Document type	Case Reports
ZDB-ID	2658169-3
ISSN	2250-0685
ISSN	2250-0685
DOI	10.13107/jocr.2023.v13.i05.3642
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Article ; Online: A standardized black pepper seed extract containing β-caryophyllene improves cognitive function in scopolamine-induced amnesia model mice via regulation of brain-derived neurotrophic factor and MAPK proteins.

Sudeep, H V / Venkatakrishna, K / Amritharaj / Gouthamchandra, K / Reethi, B / Naveen, P / Lingaraju, H B / Shyamprasad, K

Journal of food biochemistry

2021 Volume 45, Issue 12, Page(s) e13994

Abstract: β-caryophyllene (BCP), a natural sesquiterpene present in plants, is a selective agonist of cannabinoid receptor type-2 (CB2) of the endocannabinoid system. In this study, we have prepared an extract from Piper nigrum (black pepper) seeds using ... ...

Abstract	β-caryophyllene (BCP), a natural sesquiterpene present in plants, is a selective agonist of cannabinoid receptor type-2 (CB2) of the endocannabinoid system. In this study, we have prepared an extract from Piper nigrum (black pepper) seeds using supercritical fluid extraction, standardized to contain 30% BCP (Viphyllin
MeSH term(s)	Amnesia/chemically induced ; Amnesia/drug therapy ; Animals ; Brain-Derived Neurotrophic Factor ; Cognition ; Male ; Mice ; Piper nigrum ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Polycyclic Sesquiterpenes ; Scopolamine/toxicity
Chemical Substances	Brain-Derived Neurotrophic Factor ; Plant Extracts ; Polycyclic Sesquiterpenes ; caryophyllene (BHW853AU9H) ; Scopolamine (DL48G20X8X)
Language	English
Publishing date	2021-11-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	433846-7
ISSN	1745-4514 ; 0145-8884
ISSN (online)	1745-4514
ISSN	0145-8884
DOI	10.1111/jfbc.13994
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Article: Viphyllin

Venkatakrishna, Karempudi / Sundeep, Kuppam / Sudeep, Heggar Venkataramana / Gouthamchandra, Kuluvar / Shyamprasad, Kodimule

Journal of pain research

2022 Volume 15, Page(s) 355–366

Abstract: Purpose: Plant-based natural products as anti-nociceptors have enormous potential as safer alternatives to conventional opiates and NSAIDS. : Methods: In the present study, the antinociceptive activity of a standardized black pepper seed extract ( ... ...

Abstract	Purpose: Plant-based natural products as anti-nociceptors have enormous potential as safer alternatives to conventional opiates and NSAIDS. Methods: In the present study, the antinociceptive activity of a standardized black pepper seed extract (Viphyllin) containing not less than 30% β-caryophyllene (BCP) was evaluated using pain models in mice, namely acetic acid-induced writhing test, formalin-induced paw licking test, hot plate test and tail flick test. Further, the antagonists SR141716A (0.1 mg/kg i.p.), AM630 (5 mg/kg i.p.), capsazepine (0.1 mg/kg body weight i.p.), and GW6471 (1 mg/kg i.p.) were used to evaluate the involvement of cannabinoid receptors CB1 and CB2, TRPV1 ion channel and PPARα receptor, respectively. Molecular docking (AutoDock 4.2) was used to study the interaction of BCP with the agonist-binding sites of the selected pain receptors. Results: Viphyllin at 10 mg, 25 mg and 50 mg/kg (i.p.) significantly inhibited the writhings in mice as compared to untreated control group ( Conclusion: In conclusion, Viphyllin could be a natural pain-relieving agent involving safer pain signaling mechanisms, unlike conventional opiates and NSAIDs.
Language	English
Publishing date	2022-02-05
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2495284-9
ISSN	1178-7090
ISSN	1178-7090
DOI	10.2147/JPR.S351513
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Article ; Online: Viphyllin™, a standardized extract from black pepper seeds, mitigates intestinal inflammation, oxidative stress, and anxiety-like behavior in DSS-induced colitis mice.

Sudeep, Heggar Venkataramana / Venkatakrishna, Karempudi / Raj, Amritha / Reethi, Budanuru / Shyamprasad, Kodimule

Journal of food biochemistry

2022 Volume 46, Issue 10, Page(s) e14306

Abstract: Inflammatory bowel diseases (IBD) are the common health concern in populations across the world. Clinical evidence suggests that IBD, characterized by intestinal inflammation, is associated with neuronal manifestations to a greater extent. In this study, ...

Abstract	Inflammatory bowel diseases (IBD) are the common health concern in populations across the world. Clinical evidence suggests that IBD, characterized by intestinal inflammation, is associated with neuronal manifestations to a greater extent. In this study, we have investigated the protective effects of Viphyllin™, a standardized black pepper (Piper nigrum) seed extract containing 30% β-caryophyllene against dextran sodium sulfate (DSS)-induced colitis in mice. Oral pretreatment of Viphyllin at the 50 mg and 100 mg/kg doses significantly reversed the clinical symptoms of colitis in mice. Viphyllin markedly inhibited NLRP3 inflammasome activation and improved barrier function in colon tissue. Viphyllin further mitigated the DSS-induced anxiety-like behavior in mice. Interestingly, Viphyllin improved brain antioxidant status and promoted neuronal cell survival in colitis model mice. In conclusion, our findings strongly support the health claims of Viphyllin as a functional ingredient to deal with IBD and related neuronal symptoms. PRACTICAL APPLICATIONS: Prevalence of inflammatory bowel diseases is not uncommon in the modern lifestyle. Gut health is associated with neurological disorders that contribute substantially to the deterioration of quality of life and socioeconomic development. In this research work, the protective action of a black pepper seed extract standardized to 30% β-caryophyllene (Viphyllin) is evaluated against Dextran sodium sulfate-induced experimental colitis model. Here we have demonstrated the beneficial role of Viphyllin in mitigating intestinal inflammation as a function of NLRP3 inflammasome inhibition. Further, the extract improves intestinal barrier function. In an important aspect of the study, we have provided the data on the effect of Viphyllin on neurological symptoms and brain health in colitis model mice.
MeSH term(s)	Animals ; Antioxidants ; Anxiety ; Colitis/chemically induced ; Colitis/drug therapy ; Dextran Sulfate/adverse effects ; Inflammasomes ; Inflammation/drug therapy ; Inflammatory Bowel Diseases/drug therapy ; Mice ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nigella sativa/metabolism ; Oxidative Stress ; Piper nigrum/metabolism ; Plant Extracts ; Polycyclic Sesquiterpenes ; Quality of Life ; Seeds/metabolism ; Sulfates
Chemical Substances	Antioxidants ; Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; Plant Extracts ; Polycyclic Sesquiterpenes ; Sulfates ; sodium sulfate (0YPR65R21J) ; Dextran Sulfate (9042-14-2) ; caryophyllene (BHW853AU9H)
Language	English
Publishing date	2022-06-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	433846-7
ISSN	1745-4514 ; 0145-8884
ISSN (online)	1745-4514
ISSN	0145-8884
DOI	10.1111/jfbc.14306
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Article ; Online: A phytosterol-enriched saw palmetto supercritical CO

Sudeep, Heggar V / Venkatakrishna, Karempudi / Amrutharaj, Ballal / Anitha / Shyamprasad, Kodimule

BMC complementary and alternative medicine

2019 Volume 19, Issue 1, Page(s) 270

Abstract: Background: Benign prostatic hyperplasia (BPH) is a pathological condition affecting older men. BPH complications often lead to deterioration in the quality of life. Serenoa repens (Saw Palmetto) is used for treating lower urinary tract infections in ... ...

Abstract	Background: Benign prostatic hyperplasia (BPH) is a pathological condition affecting older men. BPH complications often lead to deterioration in the quality of life. Serenoa repens (Saw Palmetto) is used for treating lower urinary tract infections in traditional medicine. Methods: This study was performed to compare the efficacy of β-sitosterol enriched saw palmetto oil (VISPO) and conventional saw palmetto oil (SPO) extracted using supercritical fluid extraction, in alleviating the BPH complications using testosterone-induced BPH model rats. The animals received testosterone (5 mg/kg s.c.) with or without SPO and VISPO (200 and 400 mg/kg b.w.) or Finasteride (1 mg/kg b.w.) p.o. for 28 days. At the end of the experiment, overnight fasted animals were euthanized, blood samples collected for serum analysis of testosterone. Prostate tissue histomorphology was examined by hematoxylin and eosin (H&E) staining. Western blot analysis was performed using prostate tissue homogenates. Results: VISPO exhibited superior efficacy compared to SPO as evident from the significant decrease in prostate weight to body weight ratio, serum testosterone level and increase in growth inhibition of prostate tissue compared to BPH group (p < 0.001). Histological examination of prostate tissue samples showed that VISPO treatment was comparatively better than SPO in improving the hyperplastic patterns. Further, VISPO significantly regulated the expression of inflammatory and apoptotic marker proteins in BPH rats. Conclusion: Our data provide experimental evidence that β-sitosterol enriched saw palmetto oil could be higher efficacious in treating the BPH complications compared to the conventional saw palmetto oil preparations.
MeSH term(s)	Animals ; Chromatography, Supercritical Fluid ; Humans ; Male ; Phytosterols/administration & dosage ; Phytosterols/isolation & purification ; Phytotherapy ; Plant Extracts/administration & dosage ; Plant Extracts/isolation & purification ; Prostate/drug effects ; Prostate/immunology ; Prostatic Hyperplasia/blood ; Prostatic Hyperplasia/chemically induced ; Prostatic Hyperplasia/drug therapy ; Prostatic Hyperplasia/immunology ; Proto-Oncogene Proteins c-bcl-2/genetics ; Proto-Oncogene Proteins c-bcl-2/immunology ; Rats ; Rats, Wistar ; Serenoa/chemistry ; Sitosterols/administration & dosage ; Sitosterols/isolation & purification ; Testosterone/adverse effects ; Testosterone/blood ; bcl-2-Associated X Protein/genetics ; bcl-2-Associated X Protein/immunology
Chemical Substances	Phytosterols ; Plant Extracts ; Proto-Oncogene Proteins c-bcl-2 ; Sitosterols ; bcl-2-Associated X Protein ; Testosterone (3XMK78S47O) ; gamma-sitosterol (5LI01C78DD) ; saw palmetto extract (J7WWH9M8QS)
Language	English
Publishing date	2019-10-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/s12906-019-2697-z
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Article: A standardized black pepper seed extract containing β‐caryophyllene improves cognitive function in scopolamine‐induced amnesia model mice via regulation of brain‐derived neurotrophic factor and MAPK proteins

Sudeep, H. V. / Venkatakrishna, K. / Amritharaj / Gouthamchandra, K. / Reethi, B. / Naveen, P. / Lingaraju, H. B. / Shyamprasad, K.

Journal of food biochemistry. 2021 Dec., v. 45, no. 12

2021

Abstract: β‐caryophyllene (BCP), a natural sesquiterpene present in plants, is a selective agonist of cannabinoid receptor type‐2 (CB2) of the endocannabinoid system. In this study, we have prepared an extract from Piper nigrum (black pepper) seeds using ... ...

Abstract	β‐caryophyllene (BCP), a natural sesquiterpene present in plants, is a selective agonist of cannabinoid receptor type‐2 (CB2) of the endocannabinoid system. In this study, we have prepared an extract from Piper nigrum (black pepper) seeds using supercritical fluid extraction, standardized to contain 30% BCP (Viphyllinᵀᴹ). The beneficial effects of prophylactic treatment with Viphyllin on cognitive functions were demonstrated in Scopolamine‐induced dementia model mice. Male Swiss albino mice (25–30 g) were administered with Viphyllin (50 mg and 100 mg/kg body weight p.o.) or donepezil (1.60 mg/kg) for 14 days. Subsequently, cognitive deficits were induced by treating the animals intraperitoneally with Scopolamine (0.75 mg/kg). The cognitive behavior of mice was evaluated using a novel object recognition test (NORT) and Morris water maze (MWM) test. The brain homogenates were studied for biochemical parameters including cholinesterase activities and antioxidant status. Western blot analysis was performed to investigate the mechanism of action. Viphyllin dose dependently improved the recognition and spatial memory and cholinergic functions in Scop‐treated mice. The extract was found protective against Scop‐induced oxidative damage and histopathologic changes in the brain. At 100 mg/kg Viphyllin markedly reduced the proBDNF/mBDNF ratio (p < .05) and augmented the TrkB expression (p < .01). Viphyllin (100 mg/kg) was found to be neuroprotective by reducing the Scop‐induced upregulation of p‐JNK and p‐p38 MAPK proteins, Bax/Bcl‐2 ratio, and caspase activation in the brain. Viphyllin also exerted anti‐inflammatory effects by downregulating Cox‐2, TNF‐α, and NOS‐2 in Scop‐induced mice (p < .05). To summarize, our data encourage Viphyllin as a functional ingredient/dietary supplement for brain health and cognition. PRACTICAL APPLICATIONS: Black pepper is a culinary spice having several medicinal attributes. Essential oils in the seeds of the plant give aroma and flavor to it. Here we have prepared an extract from the seeds of black pepper using supercritical fluid extraction, characterized for the presence of β‐caryophyllene (not <30%). This research work further validates the neuroprotective mechanism of the extract in Scopolamine‐induced cognitive impairment model mice. The findings from this study strongly suggest the beneficial neuroactive properties of black pepper seed extract having the presence of BCP, a CB2 receptor agonist. It can thus be used potentially as a functional food ingredient for cognition and brain function.
Keywords	Piper nigrum ; Western blotting ; agonists ; albino ; amnesia ; antioxidants ; black pepper ; body weight ; brain ; cannabinoid receptors ; cannabinoids ; caspases ; cholinesterase ; cognition ; cognitive disorders ; dementia ; dietary supplements ; disease prevention ; flavor ; food composition ; functional foods ; histopathology ; ingredients ; males ; mechanism of action ; odors ; scopolamine ; seed extracts ; spatial memory ; supercritical fluid extraction
Language	English
Dates of publication	2021-12
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	433846-7
ISSN	1745-4514 ; 0145-8884
ISSN (online)	1745-4514
ISSN	0145-8884
DOI	10.1111/jfbc.13994
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Article: Biomechanism of chlorogenic acid complex mediated plasma free fatty acid metabolism in rat liver

H.V., Sudeep / Venkatakrishna K / Dipak Patel / Shyamprasad K

BMC complementary and alternative medicine. 2016 Dec., v. 16, no. 1

2016

Abstract: BACKGROUND: Plasma free fatty acids (FFA) are involved in blood lipid metabolism as well as many health complications. The present study was conducted to evaluate the potential role of chlorogenic acid complex from green coffee bean (CGA7) on FFA ... ...

Abstract	BACKGROUND: Plasma free fatty acids (FFA) are involved in blood lipid metabolism as well as many health complications. The present study was conducted to evaluate the potential role of chlorogenic acid complex from green coffee bean (CGA7) on FFA metabolism in high fat diet fed rats. METHODS: Hyperlipidemia was induced in Wistar rats using high-fat diet. The animals were given CGA7/orlistat concurrently for 42Â days. The parameters analysed during the study include plasma and liver total cholesterol (TC), Triglycerides (TG) and FFA. AMPK activation in the liver was analysed through ELISA. The multiple factors involved in AMPK mediated FFA metabolism were analysed using western blotting. RESULTS: CGA7 (50, 100, 150Â mg/kg BW) decreased triglycerides (TG) and FFA levels in plasma and liver. CGA7 administration led to the activation of AMP-activated protein kinase (AMPK) and a subsequent increase in the levels of carnitine palmitoyltransferase 1 (CPT-1). There was a decrease in acetyl-CoA carboxylase (ACC) activity as evident by the increase in its phosphorylation level. CONCLUSION: Chlorogenic acids improved the blood lipid metabolism in rats by alleviating the levels of FFA and TG, modulating the multiple factors in liver through AMPK pathway. The study concludes that CGA7 complex can be promoted as an active ingredient in nutrition for obesity management.
Keywords	AMP-activated protein kinase ; Western blotting ; acetyl-CoA carboxylase ; active ingredients ; alternative medicine ; blood lipids ; carnitine palmitoyltransferase ; chlorogenic acid ; cholesterol ; coffee beans ; enzyme-linked immunosorbent assay ; fatty acid metabolism ; free fatty acids ; high fat diet ; hyperlipidemia ; liver ; obesity ; phosphorylation ; rats ; triacylglycerols
Language	English
Dates of publication	2016-12
Size	p. 274.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2050429-9
ISSN	1472-6882
ISSN	1472-6882
DOI	10.1186/s12906-016-1258-y
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Article: Discrimination of normal, inflammatory, premalignant, and malignant oral tissue: a Raman spectroscopy study.

Malini, R / Venkatakrishna, K / Kurien, J / Pai, Keerthilatha M / Rao, Lakshmi / Kartha, V B / Krishna, C Murali

Biopolymers

2006 Volume 81, Issue 3, Page(s) 179–193

Abstract: Optical spectroscopy methods are fast emerging as potential alternatives for early diagnosis of cancer. A Raman spectroscopy method for discrimination of normal and malignant oral tissues has been developed by us earlier. It is necessary to evaluate and ... ...

Abstract	Optical spectroscopy methods are fast emerging as potential alternatives for early diagnosis of cancer. A Raman spectroscopy method for discrimination of normal and malignant oral tissues has been developed by us earlier. It is necessary to evaluate and establish the validity of the approach before it can be routinely used. In the present study, our Raman spectroscopy investigations are extended further to evaluate the efficacy of the technique to discriminate between normal, inflammatory, premalignant, and malignant conditions in oral tissue. Spectral profiles of normal, malignant, premalignant, and inflammatory conditions show pronounced differences between one another. Spectra of normal tissues can be attributed mainly to lipids whereas pathological tissue spectra are dominated by proteins. Principal components analysis (PCA) of the spectral data sets belonging to the four different categories showed that scores of factors differentiated between normal and all pathological conditions but gave only poor discrimination among the three pathological states. PCA combined with multiparameter limit tests allow match/mismatch criteria to be applied to test samples when pathologically certified calibration sets are available in each class. It is shown that by this method all the four tissue types could be discriminated and diagnosed correctly. The biochemical differences between normal and pathological conditions of oral tissue are also discussed from spectral differences of the different classes of spectra.
MeSH term(s)	Calibration ; Diagnosis, Differential ; Humans ; Mouth Mucosa/chemistry ; Mouth Mucosa/immunology ; Mouth Mucosa/pathology ; Mouth Neoplasms/diagnosis ; Mouth Neoplasms/pathology ; Precancerous Conditions/diagnosis ; Precancerous Conditions/pathology ; Principal Component Analysis ; Reference Values ; Spectrum Analysis, Raman ; Stomatitis/diagnosis ; Stomatitis/pathology
Language	English
Publishing date	2006-02-15
Publishing country	United States
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1123-x
ISSN	1097-0282 ; 0006-3525
ISSN (online)	1097-0282
ISSN	0006-3525
DOI	10.1002/bip.20398
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Article: Autofluorescence of oral tissue for optical pathology in oral malignancy.

Manjunath, B K / Kurein, J / Rao, L / Krishna, C Murali / Chidananda, M S / Venkatakrishna, K / Kartha, V B

Journal of photochemistry and photobiology. B, Biology

2003 Volume 73, Issue 1-2, Page(s) 49–58

Abstract: Pulsed laser-induced-fluorescence studies of pathologically certified oral tissues are carried out at different excitations and time delays. Among the several excitations used, 325 nm produced noticeably different spectral profile for normal and ... ...

Abstract	Pulsed laser-induced-fluorescence studies of pathologically certified oral tissues are carried out at different excitations and time delays. Among the several excitations used, 325 nm produced noticeably different spectral profile for normal and malignant tissues. Extensive curve analysis was carried out in order to understand changes in biochemical composition of tissue based on spectral profiles. Curve resolution and principal component analysis (PCA) show that the fluorescence intensity changes from normal to malignant tissue samples are not completely explained in terms of simple collagen and NAD(P)H intensity changes. The spectra require at least five components to be fully accounted for. Several discrimination methodologies based on PCA and intensity differences between different emission peaks (resultant peaks of curve analysis) were also evaluated. The results obtained indicate PCA using Mahalanobis distance and spectral residual as discrimination parameters provides best discrimination and can be used for matching unknown samples to standard calibration sets. Intensity ratio of bound NAD(P)H to collagen seems to be more suitable for discrimination between normal and malignant oral tissue, compared to ratio of collagen to total intensity of all the other components together.
MeSH term(s)	Fluorescence ; Humans ; Mouth Neoplasms/pathology
Language	English
Publishing date	2003-11-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	623022-2
ISSN	1873-2682 ; 1011-1344
ISSN (online)	1873-2682
ISSN	1011-1344
DOI	10.1016/j.jphotobiol.2003.09.004
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