Article: Viwithan, a Standardized
2018 Volume 13, Issue Suppl 4, Page(s) S801–S806
Abstract: Background: Withania somnifera: Objective: In the present study, we have evaluated the antiproliferative activity of a standardized : Materials and methods: The cytotoxicity of Viwithan was determined using thiazolyl blue tetrazolium blue assay ... ...
| Abstract | Background: Withania somnifera Objective: In the present study, we have evaluated the antiproliferative activity of a standardized Materials and methods: The cytotoxicity of Viwithan was determined using thiazolyl blue tetrazolium blue assay and crystal violet staining. The apoptotic changes in B16F1 cells following treatment with Viwithan were observed by acridine orange/ethidium bromide (AO/EB) staining and DNA fragmentation assay. The binding affinity of withanolides in Viwithan with antiapoptotic proteins B-cell lymphoma 2, B-cell lymphoma-extra large, and myeloid cell leukemia 1 (MCL-1) were studied using Results: The half maximal inhibitory concentration (IC50) values of Viwithan against liver hepatocellular carcinoma, Henrietta Lacks cervical carcinoma cells, human colorectal carcinoma cell line, and Ehrlich ascites carcinoma cells were 1830, 968, 2715, and 633 μg/ml, respectively. Interestingly, Viwithan was highly effective against B16F1 cells with an IC50 value of 220 μg/ml after 24 h treatment. The morphological alterations of apoptotic cell death were clearly observed in the AO/EB-stained cells after treatment with Viwithan. Viwithan induced late apoptotic changes in treated B16F1 cells as evident by the ladder formation of fragmented DNA in a time-dependent manner. The findings of molecular docking showed that withanolides present in Viwithan have a more binding affinity with the antiapoptotic proteins, particularly MCL-1. Conclusion: We have reported for the first time that Viwithan with 5% withanolides has a potent cytotoxic effect, particularly against B16F1 murine melanoma cells among the different cancer cell lines tested. Summary: The present study reports for the first time that Viwithan, a standardized 5% |
|---|---|
| Language | English |
| Publishing date | 2018-01-31 |
| Publishing country | India |
| Document type | Journal Article |
| ZDB-ID | 2274976-7 |
| ISSN | 0976-4062 ; 0973-1296 |
| ISSN (online) | 0976-4062 |
| ISSN | 0973-1296 |
| DOI | 10.4103/pm.pm_121_17 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.