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  1. Article ; Online: The parabasal filaments of

    Fiama das Neves Ortiz, Sharmila / Verdan, Raphael / Rocha, Gustavo Miranda / Miranda, Kildare / Benchimol, Marlene

    Journal of structural biology: X

    2024  Volume 9, Page(s) 100099

    Abstract: Trichomonas ... ...

    Abstract Trichomonas vaginalis
    Language English
    Publishing date 2024-03-06
    Publishing country United States
    Document type Journal Article
    ISSN 2590-1524
    ISSN (online) 2590-1524
    DOI 10.1016/j.yjsbx.2024.100099
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Trichomonas vaginalis

    Ortiz, Sharmila Fiama das Neves / Verdan, Raphael / Fortes, Fabio da Silva de Azevedo / Benchimol, Marlene

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 12

    Abstract: Trichomonas ... ...

    Abstract Trichomonas vaginalis
    Language English
    Publishing date 2023-11-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12121381
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Characterization of a new extra-axonemal structure in the Giardia intestinalis flagella.

    Verdan, Raphael / Patricio, Beatriz / Weismuller, Gilberto / Miranda, Kildare / de Souza, Wanderley / Benchimol, Marlene / Gadelha, Ana Paula

    Journal of structural biology

    2024  Volume 216, Issue 1, Page(s) 108064

    Abstract: The inner structure of the flagella of Giardia intestinalis is similar to that of other organisms, consisting of nine pairs of outer microtubules and a central pair containing radial spokes. Although the 9+2 axonemal structure is conserved, it is not ... ...

    Abstract The inner structure of the flagella of Giardia intestinalis is similar to that of other organisms, consisting of nine pairs of outer microtubules and a central pair containing radial spokes. Although the 9+2 axonemal structure is conserved, it is not clear whether subregions, including the transition zone, are present in the flagella of this parasite. Giardia axonemes originate from basal bodies and have a lengthy cytosolic portion before becoming active flagella. The region of the emergence of the flagellum is not accompanied by any membrane specialization, as seen in other protozoa. Although Giardia is an intriguing model of study, few works focused on the ultrastructural analysis of the flagella of this parasite. Here, we analyzed the externalization region of the G. intestinalis flagella using ultra-high resolution scanning microscopy (with electrons and ions), atomic force microscopy in liquid medium, freeze fracture, and electron tomography. Our data show that this region possesses a distinctive morphological feature - it extends outward and takes on a ring-like shape. When the plasma membrane is removed, a structure surrounding the axoneme becomes visible in this region. This new extra-axonemal structure is observed in all pairs of flagella of trophozoites and remains attached to the axoneme even when the interconnections between the axonemal microtubules are disrupted. High-resolution scanning electron microscopy provided insights into the arrangement of this structure, contributing to the characterization of the externalization region of the flagella of this parasite.
    MeSH term(s) Axoneme ; Giardia lamblia/ultrastructure ; Microtubules/metabolism ; Flagella/metabolism ; Microscopy, Electron, Scanning
    Language English
    Publishing date 2024-01-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1032718-6
    ISSN 1095-8657 ; 1047-8477
    ISSN (online) 1095-8657
    ISSN 1047-8477
    DOI 10.1016/j.jsb.2024.108064
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1428: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Effect of graphene oxide on bacteria and peripheral blood mononuclear cells.

    Campos-Delgado, Jessica / Castro, Kelly L S / Munguia-Lopez, Jose G / González, Ana K / Mendoza, Martin E / Fragneaud, Benjamin / Verdan, Raphael / Araujo, Joyce R / González, Francisco J / Navarro-Contreras, Hugo / Pérez-Maldonado, Ivan N / León-Rodríguez, Antonio de / Achete, Carlos A

    Journal of applied biomaterials & functional materials

    2016  Volume 14, Issue 4, Page(s) e423–e430

    Abstract: Background: Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far ... ...

    Abstract Background: Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far have only been investigated separately: GO size and exposure methodology. Our study took into account both parameters. We carefully characterized the samples to catalog sizes and structural properties, and tested different exposure methodologies: exposure in saline solution and in the presence of growth media. Furthermore, we performed experiments with peripheral blood mononuclear cells exposed to our GO materials.
    Methods: Atomic force microscopy, scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and transmission electron microscopy were used to characterize the morphology and composition of different samples of GO: GO-H2O, GO-PBS and GO-MG. Our samples had 2D sizes of ~100 nm (GO-H2O and GO-PBS) and >2 µm (GO-MG). We tested antibacterial activity and cytotoxicity toward peripheral blood mononuclear cells of 3 different GO samples.
    Results: A size-dependent growth inhibition of Escherichia coli (DH5 α) in suspension was found, which proved that this effect depends strongly on the protocol followed for exposure. Hemocompatibility was confirmed by exposing peripheral blood mononuclear cells to materials for 24 hours; viability and apoptosis tests were also carried out.
    Conclusions: Our experiments provide vital information for future applications of GO in suspension. If its antibacterial properties are to be potentiated, care should be taken to select 2D sizes in the micrometer range, and exposure should not be carried out in the presence of grow media.
    MeSH term(s) Apoptosis/drug effects ; Cell Survival/drug effects ; Escherichia coli/growth & development ; Escherichia coli/ultrastructure ; Graphite/pharmacology ; Humans ; Leukocytes, Mononuclear/metabolism ; Leukocytes, Mononuclear/ultrastructure ; Microbial Viability/drug effects
    Chemical Substances Graphite (7782-42-5)
    Language English
    Publishing date 2016-11-02
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2673624-X
    ISSN 2280-8000 ; 1722-6899
    ISSN (online) 2280-8000
    ISSN 1722-6899
    DOI 10.5301/jabfm.5000325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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