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  1. Article ; Online: Intracellular activity and

    Mascary, Jean-Baptiste / Bordeau, Valérie / Nicolas, Irène / Verdier, Marie-Clémence / Rocheteau, Pierre / Cattoir, Vincent

    JAC-antimicrobial resistance

    2024  Volume 6, Issue 1, Page(s) dlae025

    Abstract: Objectives: Assessing the therapeutic potential of a novel antimicrobial pseudopeptide, Pep16, both : Methods: Seven clinical isolates of : Results: MICs of Pep16 were consistently at 8 mg/L for all clinical isolates of : Conclusions: Pep16 is ... ...

    Abstract Objectives: Assessing the therapeutic potential of a novel antimicrobial pseudopeptide, Pep16, both
    Methods: Seven clinical isolates of
    Results: MICs of Pep16 were consistently at 8 mg/L for all clinical isolates of
    Conclusions: Pep16 is a promising option for the treatment of septic arthritis due to
    Language English
    Publishing date 2024-02-26
    Publishing country England
    Document type Journal Article
    ISSN 2632-1823
    ISSN (online) 2632-1823
    DOI 10.1093/jacamr/dlae025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Transient Lactic Acidosis and Elevation of Transaminases after the Introduction of Remdesivir in a Patient with Acute Kidney Injury.

    André, Elise / Lemaitre, Florian / Verdier, Marie-Clémence / Haufroid, Vincent / Pereira, João Pinto / Hantson, Philippe

    Case reports in critical care

    2024  Volume 2024, Page(s) 6631866

    Abstract: A 56-year-old woman was transferred to the intensive care unit (ICU) two days after an allogeneic stem cell transplantation (ASCT) when she presented acute respiratory distress due to the relapse of a SARS-CoV-2 infection. Following that, she received ... ...

    Abstract A 56-year-old woman was transferred to the intensive care unit (ICU) two days after an allogeneic stem cell transplantation (ASCT) when she presented acute respiratory distress due to the relapse of a SARS-CoV-2 infection. Following that, she received two intravenous doses of 100 mg remdesivir. Subsequently, the patient developed multiple instances of diarrhea, progressing to oliguria and acute kidney injury, necessitating continuous venovenous hemofiltration (CVVH). Despite the absence of signs of hypoxemia or cardiocirculatory failure requiring vasopressor intervention, a progressive lactic acidosis emerged. Two days after the onset of lactic acidosis, a significant rise in aminotransferases and lactate dehydrogenase occurred, in the absence of encephalopathy and coagulation disorders. Remdesivir therapy had been interrupted upon the initial signs of lactic acidosis. Despite an improvement in liver function tests and lactic acidosis, the patient's condition deteriorated, ultimately leading to her demise on day 29 due to newly arising hematological complications.
    Language English
    Publishing date 2024-02-22
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2927720-6
    ISSN 2090-6439 ; 2090-6420
    ISSN (online) 2090-6439
    ISSN 2090-6420
    DOI 10.1155/2024/6631866
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: First Experience of Optimization of Tacrolimus Therapeutic Drug Monitoring in a Patient Cotreated With Nirmatrelvir/Ritonavir: How Microsampling Approach Changes Everything.

    Golbin, Léonard / Tron, Camille / Franck, Bénédicte / Vigneau, Cécile / Verdier, Marie-Clémence / Lemaitre, Florian

    Transplantation

    2023  Volume 107, Issue 2, Page(s) e68–e69

    MeSH term(s) Humans ; Ritonavir/therapeutic use ; Tacrolimus/adverse effects ; Drug Monitoring
    Chemical Substances Ritonavir (O3J8G9O825) ; nirmatrelvir (7R9A5P7H32) ; Tacrolimus (WM0HAQ4WNM)
    Language English
    Publishing date 2023-01-26
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 208424-7
    ISSN 1534-6080 ; 0041-1337
    ISSN (online) 1534-6080
    ISSN 0041-1337
    DOI 10.1097/TP.0000000000004430
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Differential response to antibiotic therapy in staphylococcal infective endocarditis: contribution of an ex vivo model.

    Lalanne, Sébastien / Cattoir, Vincent / Guerin, François / Verdier, Marie-Clémence / Revest, Matthieu

    The Journal of antimicrobial chemotherapy

    2023  Volume 78, Issue 7, Page(s) 1689–1693

    Abstract: Objectives: Staphylococcal infective endocarditis (IE) remains a hard-to-treat infection with high mortality. Both the evaluation of new innovative therapies and research on alternative models mimicking human IE are therefore urgently needed to improve ... ...

    Abstract Objectives: Staphylococcal infective endocarditis (IE) remains a hard-to-treat infection with high mortality. Both the evaluation of new innovative therapies and research on alternative models mimicking human IE are therefore urgently needed to improve the prognosis of patients with diagnosed IE. Dalbavancin is a novel anti-staphylococcal lipoglycopeptide but there are limited data supporting its efficacy on biofilm infections. This antibiotic could be an alternative to current therapies for the medical treatment of IE but it needs to be further evaluated.
    Methods: Here we developed an original ex vivo model of Staphylococcus aureus IE on human heart valves and assessed biofilm formation on them. After validating the model, the efficacy of two antistaphylococcal antibiotics, vancomycin and dalbavancin, was compared by measuring and visualizing their respective ability to inhibit and eradicate late-formed biofilm.
    Results: Determination of the minimum biofilm inhibitory (MbIC) and eradicating (MbEC) concentrations in our ex vivo model identified dalbavancin as a promising drug with much lower MbIC and MBEC than vancomycin (respectively <0.01 versus 28 mg/L and 0.03 versus 32 mg/L).
    Conclusions: These data highlight a strong bactericidal effect of dalbavancin, particularly on an infected heart valve compared with vancomycin. Dalbavancin could be a realistic alternative treatment for the management of staphylococcal IE.
    MeSH term(s) Humans ; Vancomycin/pharmacology ; Vancomycin/therapeutic use ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Staphylococcal Infections/drug therapy ; Endocarditis, Bacterial/drug therapy ; Microbial Sensitivity Tests ; Endocarditis/drug therapy
    Chemical Substances Vancomycin (6Q205EH1VU) ; methyl 2-(-5-fluoro-2-hydroxyphenyl)-1 H-benzo(d)imidazole-5-carboxylate ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-05-29
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkad155
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Lactic acidosis after allogeneic haematopoietic stem cell transplantation potentially related to letermovir.

    Manczak, Bérénice / Verdier, Marie-Clémence / Dewulf, Joseph P / Lemaitre, Florian / Haufroid, Vincent / Hantson, Philippe

    British journal of clinical pharmacology

    2023  Volume 89, Issue 5, Page(s) 1686–1689

    Abstract: A 53-year-old woman with a history of acute myeloid leukaemia received a second allogeneic haematopoietic stem cell transplant and was prescribed, among other medications, acyclovir and letermovir (480-mg daily oral dose) for prophylaxis of, respectively, ...

    Abstract A 53-year-old woman with a history of acute myeloid leukaemia received a second allogeneic haematopoietic stem cell transplant and was prescribed, among other medications, acyclovir and letermovir (480-mg daily oral dose) for prophylaxis of, respectively, herpes simplex and cytomegalovirus infection. The patient was admitted in the intensive care unit for dyspnoea and oliguria. Laboratory investigations revealed acute kidney injury but also a severe and progressive lactic acidosis. Liver function tests were within normal range. The combination of lactic acidosis, hypoglycaemia and acylcarnitine profile in plasma raised the suspicion of mitochondrial toxicity. Letermovir therapy was interrupted, and determination of plasma letermovir pharmacokinetics revealed a prolonged terminal half-life (38.7 h) that was not significantly influenced by continuous venovenous haemofiltration. Exploration for genetic polymorphisms revealed that the patient was SLCO1B1*5/*15 (c.521T>C homozygous carrier and c.388A>G heterozygous carrier) with a predicted nonfunctional organic anion transporting polypeptide 1B1 protein. The relationship between letermovir accumulation and development of lactic acidosis requires further observations.
    MeSH term(s) Female ; Humans ; Middle Aged ; Acidosis, Lactic/therapy ; Acidosis, Lactic/drug therapy ; Antiviral Agents/therapeutic use ; Cytomegalovirus Infections/drug therapy ; Acetates/pharmacokinetics ; Hematopoietic Stem Cell Transplantation/adverse effects ; Liver-Specific Organic Anion Transporter 1
    Chemical Substances letermovir (1H09Y5WO1F) ; Antiviral Agents ; Acetates ; SLCO1B1 protein, human ; Liver-Specific Organic Anion Transporter 1
    Language English
    Publishing date 2023-02-19
    Publishing country England
    Document type Case Reports
    ZDB-ID 188974-6
    ISSN 1365-2125 ; 0306-5251 ; 0264-3774
    ISSN (online) 1365-2125
    ISSN 0306-5251 ; 0264-3774
    DOI 10.1111/bcp.15686
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Clinical trials during pandemics and beyond: time for a more efficient pharmacological strategy.

    Lemaitre, Florian / Locher, Clara / Verdier, Marie-Clémence / Naudet, Florian

    The Journal of antimicrobial chemotherapy

    2021  Volume 76, Issue 9, Page(s) 2234–2236

    Abstract: During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, clinical trials on antiviral or symptomatic drugs have been conducted very rapidly even for drugs with a poor pharmacological rationale for efficacy on SARS-CoV-2. Despite ... ...

    Abstract During the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, clinical trials on antiviral or symptomatic drugs have been conducted very rapidly even for drugs with a poor pharmacological rationale for efficacy on SARS-CoV-2. Despite lacking basic pharmacological information, most of these clinical trials were also extremely redundant. Applying simple rules, (such as identifying a mechanistic rationale, confirming the ability to reach exposure targets at therapeutic dosage and ensuring tests show drug efficacy in appropriate in vitro and animal models before entering clinical trials) might have saved considerable amounts of time and money, and might have avoided useless research. Moreover, combining these simple rules with the implementation of a relevant policy at both an international and a national level, by limiting studies with a poor methodological/scientific approach and aggregating studies with similar design into single clinical trials, is potentially a far more-efficient strategy.
    MeSH term(s) Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; COVID-19 ; Clinical Trials as Topic ; Humans ; Pandemics ; SARS-CoV-2
    Chemical Substances Antiviral Agents
    Language English
    Publishing date 2021-06-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkab190
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Amoxicillin-Induced Neurotoxicity: Contribution of a Healthcare Data Warehouse to the Determination of a Toxic Concentration Threshold.

    Lalanne, Sébastien / Bouzillé, Guillaume / Tron, Camille / Revest, Matthieu / Polard, Elisabeth / Bellissant, Eric / Verdier, Marie-Clémence / Lemaitre, Florian

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 4

    Abstract: Background: Amoxicillin (AMX)-induced neurotoxicity is well described and may be associated with AMX overexposure. No neurotoxic concentration threshold has been determined thus far. A better knowledge of maximum tolerable AMX concentrations is of ... ...

    Abstract Background: Amoxicillin (AMX)-induced neurotoxicity is well described and may be associated with AMX overexposure. No neurotoxic concentration threshold has been determined thus far. A better knowledge of maximum tolerable AMX concentrations is of importance to improve the safety of high doses of AMX.
    Methods: We conducted a retrospective study using the local hospital data warehouse EhOP
    Results: The query identified 101 patients among 2054 patients benefiting from AMX TDM. Patients received a median daily dose of 9 g AMX, with a median creatinine clearance of 51 mL/min. A total of 17 of the 101 patients exhibited neurotoxicity attributed to AMX. The mean Css was higher for patients with neurotoxicity attributed to AMX (118 ± 62 mg/L) than those without 74 ± 48 mg/L (
    Conclusions: This study identified, for the first time, an AMX Css threshold of 109.7 mg/L associated with an excess risk of neurotoxicity. This approach needs to be confirmed by a prospective study with systematic neurological evaluation and TDM.
    Language English
    Publishing date 2023-03-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12040680
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Amoxicillin therapeutic drug monitoring for endocarditis: A comparative study (EI-STAB).

    Dorel, Marie / Albert, Robin / Le Bot, Audrey / Caillault, Leila / Lalanne, Sébastien / Tattevin, Pierre / Verdier, Marie-Clémence / Lemaignen, Adrien / Revest, Matthieu

    International journal of antimicrobial agents

    2023  Volume 62, Issue 1, Page(s) 106821

    Abstract: Introduction: International guidelines recommend high doses of β-lactams for most cases of infective endocarditis (IE). Therapeutic drug monitoring (TDM) is increasingly used to adjust β-lactam dose based on plasma concentrations, although there are no ... ...

    Abstract Introduction: International guidelines recommend high doses of β-lactams for most cases of infective endocarditis (IE). Therapeutic drug monitoring (TDM) is increasingly used to adjust β-lactam dose based on plasma concentrations, although there are no comparative studies to support this practice. The benefit of amoxicillin TDM during IE was evaluated.
    Methods: An observational, retrospective, cohort study of adults treated with high-dose amoxicillin for enterococcal or streptococcal IE was conducted in two referral centers. Patients with, or without TDM were compared. The primary outcome was mean daily amoxicillin dose.
    Results: A total of 206 cases of streptococcal (n=140, 68%) or enterococcal (n=66, 32%) IE were included. IE occurred on prosthetic valves in 77 (37%) cases, and on intracardiac devices in 28 (14%) cases. Aortic valve was involved in 136 (66%) cases. There were 154 men (75%), mean age was 70 ± 14 years, valve surgery was performed in 81/206 (39%) patients, and in-hospital mortality was 8% (17/206). All patients in the TDM group and most patients in the group without TDM received amoxicillin as continuous infusion. Amoxicillin TDM was performed for 114 patients (55.3%), with a mean of 4.7 ± 2.3 measures per patient, a mean plasma steady-state concentration of 41.2 ± 19 mg/L, most (82/114, 72%) being within the therapeutic target (20-80 mg/L). Mean amoxicillin dose was lower in patients with TDM (10.0 ± 3.3 g/day) than those without TDM (11.3 ± 2.0 g/day) (P=0.003).
    Conclusion: Amoxicillin TDM was associated with a reduction in daily doses, with no impact on adverse events and prognosis. Individualized treatment of IE through TDM may contribute to decreased use of antibiotics.
    MeSH term(s) Adult ; Male ; Humans ; Middle Aged ; Aged ; Aged, 80 and over ; Amoxicillin/therapeutic use ; Cohort Studies ; Retrospective Studies ; Drug Monitoring ; Anti-Bacterial Agents/therapeutic use ; Endocarditis/drug therapy ; Endocarditis, Bacterial/drug therapy ; Streptococcus ; beta-Lactams/therapeutic use ; Enterococcus
    Chemical Substances Amoxicillin (804826J2HU) ; Anti-Bacterial Agents ; beta-Lactams
    Language English
    Publishing date 2023-04-21
    Publishing country Netherlands
    Document type Observational Study ; Journal Article
    ZDB-ID 1093977-5
    ISSN 1872-7913 ; 0924-8579
    ISSN (online) 1872-7913
    ISSN 0924-8579
    DOI 10.1016/j.ijantimicag.2023.106821
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Amoxicillin therapeutic drug monitoring for endocarditis: A comparative study (EI-STAB)

    Dorel, Marie / Albert, Robin / Le Bot, Audrey / Caillault, Leila / Lalanne, Sébastien / Tattevin, Pierre / Verdier, Marie-Clémence / Lemaignen, Adrien / Revest, Matthieu

    International Journal of Antimicrobial Agents. 2023 July, v. 62, no. 1 p.106821-

    2023  

    Abstract: International guidelines recommend high doses of β-lactams for most cases of infective endocarditis (IE). Therapeutic drug monitoring (TDM) is increasingly used to adjust β-lactam dose based on plasma concentrations, although there are no comparative ... ...

    Abstract International guidelines recommend high doses of β-lactams for most cases of infective endocarditis (IE). Therapeutic drug monitoring (TDM) is increasingly used to adjust β-lactam dose based on plasma concentrations, although there are no comparative studies to support this practice. The benefit of amoxicillin TDM during IE was evaluated. An observational, retrospective, cohort study of adults treated with high-dose amoxicillin for enterococcal or streptococcal IE was conducted in two referral centers. Patients with, or without TDM were compared. The primary outcome was mean daily amoxicillin dose. A total of 206 cases of streptococcal (n=140, 68%) or enterococcal (n=66, 32%) IE were included. IE occurred on prosthetic valves in 77 (37%) cases, and on intracardiac devices in 28 (14%) cases. Aortic valve was involved in 136 (66%) cases. There were 154 men (75%), mean age was 70 ± 14 years, valve surgery was performed in 81/206 (39%) patients, and in-hospital mortality was 8% (17/206). All patients in the TDM group and most patients in the group without TDM received amoxicillin as continuous infusion. Amoxicillin TDM was performed for 114 patients (55.3%), with a mean of 4.7 ± 2.3 measures per patient, a mean plasma steady-state concentration of 41.2 ± 19 mg/L, most (82/114, 72%) being within the therapeutic target (20–80 mg/L). Mean amoxicillin dose was lower in patients with TDM (10.0 ± 3.3 g/day) than those without TDM (11.3 ± 2.0 g/day) (P=0.003). Amoxicillin TDM was associated with a reduction in daily doses, with no impact on adverse events and prognosis. Individualized treatment of IE through TDM may contribute to decreased use of antibiotics.
    Keywords amoxicillin ; cohort studies ; comparative study ; endocarditis ; mortality ; patients ; prognosis ; prostheses ; surgery ; Infective endocarditis ; Therapeutic drug monitoring ; Streptococcus ; Enterocococcus
    Language English
    Dates of publication 2023-07
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 1093977-5
    ISSN 1872-7913 ; 0924-8579
    ISSN (online) 1872-7913
    ISSN 0924-8579
    DOI 10.1016/j.ijantimicag.2023.106821
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: A Robust and Fast/Multiplex Pharmacogenetics Assay to Simultaneously Analyze 17 Clinically Relevant Genetic Polymorphisms in

    Tron, Camille / Bouvet, Régis / Verdier, Marie-Clémence / Lamoureux, Fabien / Hennart, Benjamin / Dubourg, Christèle / Bellissant, Eric / Galibert, Marie-Dominique

    Pharmaceuticals (Basel, Switzerland)

    2022  Volume 15, Issue 5

    Abstract: In the field of pharmacogenetics, the trend is to analyze a panel of several actionable genetic polymorphisms. It may require the use of high-throughput sequencing which demands expensive reagents/instruments and specific skills to interpret results. As ... ...

    Abstract In the field of pharmacogenetics, the trend is to analyze a panel of several actionable genetic polymorphisms. It may require the use of high-throughput sequencing which demands expensive reagents/instruments and specific skills to interpret results. As an alternative, the aim of this work was to validate an easy, fast, and inexpensive multiplex pharmacogenetics assay to simultaneously genotype a panel of 17 clinically actionable variants involved in drug pharmacokinetics/pharmacodynamics. We designed primers to perform a multiplex PCR assay using a single mix. Primers were labeled by two fluorescent dye markers to discriminate alleles, while the size of the PCR fragments analyzed by electrophoresis allowed identifying amplicon. Polymorphisms of interest were CYP3A4*22, CYP3A5*3, CYP1A2*1F, CYP2C9*2-*3, CYP2C19*2-*3-*17, VKORC1-1639G > A, ABCB1 rs1045642-rs1128503-rs2229109-rs2032582, and CYP2D6*3-*4-*6-*9. The assay was repeatable and a minimum quantity of 10 ng of DNA/ sample was needed to obtain accurate results. The method was applied to a validation cohort of 121 samples and genotyping results were consistent with those obtained with reference methods. The assay was fast and cost-effective with results being available within one working-day. This robust assay can easily be implemented in laboratories as an alternative to cumbersome simplex assays or expensive multiplex approaches. Together it should widespread access to pharmacogenetics in clinical routine practice.
    Language English
    Publishing date 2022-05-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph15050637
    Database MEDical Literature Analysis and Retrieval System OnLINE

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