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  1. Article: Cell line-specific accumulation of the baculovirus non-hr origin of DNA replication in infected insect cells.

    Pijlman, Gorben P / Vermeesch, Angela M G / Vlak, Just M

    Journal of invertebrate pathology

    2003  Volume 84, Issue 3, Page(s) 214–219

    Abstract: Successive viral passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in the S. exigua cell line Se301 leads to the rapid accumulation of the non-hr origin of DNA replication (ori) as large concatemers. Passage of SeMNPV in two other S. ...

    Abstract Successive viral passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in the S. exigua cell line Se301 leads to the rapid accumulation of the non-hr origin of DNA replication (ori) as large concatemers. Passage of SeMNPV in two other S. exigua cell lines, SeUCR1 and SeIZD2109, did not show the accumulation of such concatemers. When introduced into SeUCR1 and SeIZD2109 cells, the non-hr ori concatemers generated in Se301 cells were maintained but did not increase. This suggests that the non-hr ori confers a strong selective advantage in Se301 cells, but not or to a lesser extent in the other cell lines. The cell line-specific accumulation of non-hr ori concatemers might be due to a higher intrinsic recombination frequency in Se301 cells and may reflect tissue related differences involving some host cell factor(s). Since non-hr ori concatemers in Se301 cells were more abundant in intracellular than in extracellular viral DNA preparations, episomal replication and the requirement of a minimal DNA size for packaging into nucleocapsids is hypothesized.
    MeSH term(s) Animals ; Blotting, Southern ; Cell Line ; DNA, Concatenated ; DNA, Viral/genetics ; Nucleopolyhedrovirus/genetics ; Replication Origin/genetics ; Spodoptera/virology
    Chemical Substances DNA, Concatenated ; DNA, Viral
    Language English
    Publishing date 2003-11-05
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390885-9
    ISSN 1096-0805 ; 0022-2011
    ISSN (online) 1096-0805
    ISSN 0022-2011
    DOI 10.1016/j.jip.2003.10.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 958: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins.

    van Hulten, Mariëlle C W / Reijns, Martin / Vermeesch, Angela M G / Zandbergen, Fokko / Vlak, Just M

    The Journal of general virology

    2002  Volume 83, Issue Pt 1, Page(s) 257–265

    Abstract: White spot syndrome virus (WSSV) infects penaeid shrimp and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double-stranded DNA genome of 293 kbp. The virion envelope contains two major ... ...

    Abstract White spot syndrome virus (WSSV) infects penaeid shrimp and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double-stranded DNA genome of 293 kbp. The virion envelope contains two major proteins of 28 (VP28) and 19 kDa (VP19) and the nucleocapsid consists of three major proteins of 26 (VP26), 24 (VP24) and 15 kDa (VP15). Study on the morphogenesis of the WSSV particle requires the genomic identification and chemical characterization of these WSSV virion proteins. An internal amino acid sequence of envelope protein VP19 was obtained by amino acid sequencing and used to locate the VP19 open reading frame of this protein on the genome, as WSSV ORF182. VP19 contained two putative transmembrane domains, which may anchor this protein in the WSSV envelope. Similarly, the gene for VP15 was located on the WSSV genome as ORF109. N-terminal amino acid sequencing on VP15 suggested that this protein was expressed from the second ATG of its ORF and the first methionine is lost by N-terminal protein processing. The 15 kDa protein is very basic and is a candidate DNA-binding protein in the WSSV nucleocapsid. None of the five major structural WSSV proteins appear to be glycosylated, which is an unusual feature among enveloped animal viruses.
    MeSH term(s) Amino Acid Sequence ; Animals ; Astacoidea ; Base Sequence ; Cell Line ; DNA Viruses/genetics ; DNA Viruses/metabolism ; DNA, Viral ; Decapoda/virology ; Genes, Viral ; Genome, Viral ; Glycosylation ; Molecular Sequence Data ; Nucleocapsid Proteins/genetics ; Nucleocapsid Proteins/metabolism ; Viral Envelope Proteins/genetics ; Viral Envelope Proteins/metabolism ; Virion
    Chemical Substances DNA, Viral ; Nucleocapsid Proteins ; VP15 protein, white spot syndrome virus ; VP19 protein, white spot syndrome virus ; Viral Envelope Proteins
    Language English
    Publishing date 2002-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 219316-4
    ISSN 1465-2099 ; 0022-1317
    ISSN (online) 1465-2099
    ISSN 0022-1317
    DOI 10.1099/0022-1317-83-1-257
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 610: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Pivotal role of the non-hr origin of DNA replication in the genesis of defective interfering baculoviruses.

    Pijlman, Gorben P / Dortmans, Jos C F M / Vermeesch, Angela M G / Yang, Kai / Martens, Dirk E / Goldbach, Rob W / Vlak, Just M

    Journal of virology

    2002  Volume 76, Issue 11, Page(s) 5605–5611

    Abstract: The generation of deletion mutants, including defective interfering viruses, upon serial passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in insect cell culture has been studied. Sequences containing the non-homologous region origin ...

    Abstract The generation of deletion mutants, including defective interfering viruses, upon serial passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in insect cell culture has been studied. Sequences containing the non-homologous region origin of DNA replication (non-hr ori) became hypermolar in intracellular viral DNA within 10 passages in Se301 insect cells, concurrent with a dramatic drop in budded virus and polyhedron production. These predominant non-hr ori-containing sequences accumulated in larger concatenated forms and were generated de novo as demonstrated by their appearance and accumulation upon infection with a genetically homogeneous bacterial clone of SeMNPV (bacmid). Sequences were identified at the junctions of the non-hr ori units within the concatemers, which may be potentially involved in recombination events. Deletion of the SeMNPV non-hr ori using RecE/RecT-mediated homologous ET recombination in Escherichia coli resulted in a recombinant bacmid with strongly enhanced stability of virus and polyhedron production upon serial passage in insect cells. This suggests that the accumulation of non-hr oris upon passage is due to the replication advantage of these sequences. The non-hr ori deletion mutant SeMNPV bacmid can be exploited as a stable eukaryotic heterologous protein expression vector in insect cells.
    MeSH term(s) Animals ; Cell Line ; DNA Replication ; DNA, Viral/physiology ; Defective Viruses/genetics ; Defective Viruses/physiology ; Nucleopolyhedroviruses/genetics ; Nucleopolyhedroviruses/physiology ; Replication Origin/physiology ; Spodoptera/virology ; Virus Replication/physiology
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2002-03-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.76.11.5605-5611.2002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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