LIVIVO - Search results -

Search results

Result 1 - 10 of total 10

Search options

Article ; Online: A regulatory circuit controlled by extranuclear and nuclear retinoic acid receptor α determines T cell activation and function.

Larange, Alexandre / Takazawa, Ikuo / Kakugawa, Kiyokazu / Thiault, Nicolas / Ngoi, SooMun / Olive, Meagan E / Iwaya, Hitoshi / Seguin, Laetitia / Vicente-Suarez, Ildefonso / Becart, Stephane / Verstichel, Greet / Balancio, Ann / Altman, Amnon / Chang, John T / Taniuchi, Ichiro / Lillemeier, Bjorn / Kronenberg, Mitchell / Myers, Samuel A / Cheroutre, Hilde

Immunity

2023 Volume 56, Issue 9, Page(s) 2054–2069.e10

Abstract: Ligation of retinoic acid receptor alpha (RARα) by RA promotes varied transcriptional programs associated with immune activation and tolerance, but genetic deletion approaches suggest the impact of RARα on TCR signaling. Here, we examined whether RARα ... ...

Abstract	Ligation of retinoic acid receptor alpha (RARα) by RA promotes varied transcriptional programs associated with immune activation and tolerance, but genetic deletion approaches suggest the impact of RARα on TCR signaling. Here, we examined whether RARα would exert roles beyond transcriptional regulation. Specific deletion of the nuclear isoform of RARα revealed an RARα isoform in the cytoplasm of T cells. Extranuclear RARα was rapidly phosphorylated upon TCR stimulation and recruited to the TCR signalosome. RA interfered with extranuclear RARα signaling, causing suboptimal TCR activation while enhancing FOXP3
MeSH term(s)	Humans ; Retinoic Acid Receptor alpha/genetics ; Lymphocyte Activation ; Autoimmune Diseases ; Cell Membrane ; Receptors, Antigen, T-Cell
Chemical Substances	Retinoic Acid Receptor alpha ; Receptors, Antigen, T-Cell
Language	English
Publishing date	2023-08-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	1217235-2
ISSN	1097-4180 ; 1074-7613
ISSN (online)	1097-4180
ISSN	1074-7613
DOI	10.1016/j.immuni.2023.07.017
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 4227: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 69: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: CD4

Dicker, Martina / Li, Yingcong / Giles, Daniel A / Verstichel, Greet / Castelan, Viankail Cedillo / Ascui-Gac, Gabriel / Chou, Ting-Fang / Perez-Jeldres, Tamara / Cheroutre, Hilde / Kronenberg, Mitchell

Frontiers in immunology

2022 Volume 13, Page(s) 1034648

Abstract: Colitis is characterized by an exacerbated intestinal immune response, but the genetic and other mechanisms regulating immune activation remain incompletely understood. In order to identify new pathways leading to colitis, we sought to identify genes ... ...

Abstract	Colitis is characterized by an exacerbated intestinal immune response, but the genetic and other mechanisms regulating immune activation remain incompletely understood. In order to identify new pathways leading to colitis, we sought to identify genes with increased expression in the colons of patients that also are near loci identified by genome wide association studies (GWAS) associated with IBD risk. One such SNP, rs9557195 was of particular interest because it is within an intron of
MeSH term(s)	Mice ; Humans ; Animals ; Dextran Sulfate/adverse effects ; Genome-Wide Association Study ; Mice, Inbred C57BL ; Colitis/chemically induced ; Colitis/genetics ; Disease Models, Animal ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; CD4-Positive T-Lymphocytes/metabolism
Chemical Substances	Dextran Sulfate (9042-14-2) ; Receptors, G-Protein-Coupled ; GPR183 protein, human ; GPR18 protein, human ; Gpr183 protein, mouse ; GPR18 protein, mouse
Language	English
Publishing date	2022-10-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1034648
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Transcriptomes and metabolism define mouse and human MAIT cell populations.

Chandra, Shilpi / Ascui, Gabriel / Riffelmacher, Thomas / Chawla, Ashu / Ramírez-Suástegui, Ciro / Castelan, Viankail C / Seumois, Gregory / Simon, Hayley / Murray, Mallory P / Seo, Goo-Young / Premlal, Ashmitaa L R / Schmiedel, Benjamin / Verstichel, Greet / Li, Yingcong / Lin, Chia-Hao / Greenbaum, Jason / Lamberti, John / Murthy, Raghav / Nigro, John /

Cheroutre, Hilde / Ottensmeier, Christian H / Hedrick, Stephen M / Lu, Li-Fan / Vijayanand, Pandurangan / Kronenberg, Mitchell

Science immunology

2023 Volume 8, Issue 89, Page(s) eabn8531

Abstract: Mucosal-associated invariant T (MAIT) cells are a subset of T lymphocytes that respond to microbial metabolites. We defined MAIT cell populations in different organs and characterized the developmental pathway of mouse and human MAIT cells in the thymus ... ...

Abstract	Mucosal-associated invariant T (MAIT) cells are a subset of T lymphocytes that respond to microbial metabolites. We defined MAIT cell populations in different organs and characterized the developmental pathway of mouse and human MAIT cells in the thymus using single-cell RNA sequencing and phenotypic and metabolic analyses. We showed that the predominant mouse subset, which produced IL-17 (MAIT17), and the subset that produced IFN-γ (MAIT1) had not only greatly different transcriptomes but also different metabolic states. MAIT17 cells in different organs exhibited increased lipid uptake, lipid storage, and mitochondrial potential compared with MAIT1 cells. All these properties were similar in the thymus and likely acquired there. Human MAIT cells in lung and blood were more homogeneous but still differed between tissues. Human MAIT cells had increased fatty acid uptake and lipid storage in blood and lung, similar to human CD8 T resident memory cells, but unlike mouse MAIT17 cells, they lacked increased mitochondrial potential. Although mouse and human MAIT cell transcriptomes showed similarities for immature cells in the thymus, they diverged more strikingly in the periphery. Analysis of pet store mice demonstrated decreased lung MAIT17 cells in these so-called "dirty" mice, indicative of an environmental influence on MAIT cell subsets and function.
MeSH term(s)	Humans ; Mucosal-Associated Invariant T Cells ; Transcriptome ; CD8-Positive T-Lymphocytes ; Thymus Gland ; Lipids
Chemical Substances	Lipids
Language	English
Publishing date	2023-11-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	2470-9468
ISSN (online)	2470-9468
DOI	10.1126/sciimmunol.abn8531
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Van Caeneghem, Yasmine / De Munter, Stijn / Tieppo, Paola / Goetgeluk, Glenn / Weening, Karin / Verstichel, Greet / Bonte, Sarah / Taghon, Tom / Leclercq, Georges / Kerre, Tessa / Debets, Reno / Vermijlen, David / Abken, Hinrich / Vandekerckhove, Bart

Oncoimmunology

2017 Volume 6, Issue 3, Page(s) e1283460

Abstract: Recent clinical studies indicate that adoptive T-cell therapy and especially chimeric antigen receptor (CAR) T-cell therapy is a very potent and potentially curative treatment for B-lineage hematologic malignancies. Currently, autologous peripheral blood ...

Abstract	Recent clinical studies indicate that adoptive T-cell therapy and especially chimeric antigen receptor (CAR) T-cell therapy is a very potent and potentially curative treatment for B-lineage hematologic malignancies. Currently, autologous peripheral blood T cells are used for adoptive T-cell therapy. Adoptive T cells derived from healthy allogeneic donors may have several advantages; however, the expected occurrence of graft versus host disease (GvHD) as a consequence of the diverse allogeneic T-cell receptor (TCR) repertoire expressed by these cells compromises this approach. Here, we generated T cells from cord blood hematopoietic progenitor cells (HPCs) that were transduced to express an antigen receptor (AR): either a CAR or a TCR with or without built-in CD28 co-stimulatory domains. These AR-transgenic HPCs were culture-expanded on an OP9-DL1 feeder layer and subsequently differentiated to CD5
Language	English
Publishing date	2017-01-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2645309-5
ISSN	2162-402X ; 2162-4011
ISSN (online)	2162-402X
ISSN	2162-4011
DOI	10.1080/2162402X.2017.1283460
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Notch induces human T-cell receptor γδ+ thymocytes to differentiate along a parallel, highly proliferative and bipotent CD4 CD8 double-positive pathway.

Van Coppernolle, S / Vanhee, S / Verstichel, G / Snauwaert, S / van der Spek, A / Velghe, I / Sinnesael, M / Heemskerk, M H / Taghon, T / Leclercq, G / Plum, J / Langerak, A W / Kerre, T / Vandekerckhove, B

Leukemia

2012 Volume 26, Issue 1, Page(s) 127–138

Abstract: In wild-type mice, T-cell receptor (TCR) γδ(+) cells differentiate along a CD4 CD8 double-negative (DN) pathway whereas TCRαβ(+) cells differentiate along the double-positive (DP) pathway. In the human postnatal thymus (PNT), DN, DP and single-positive ( ... ...

Abstract	In wild-type mice, T-cell receptor (TCR) γδ(+) cells differentiate along a CD4 CD8 double-negative (DN) pathway whereas TCRαβ(+) cells differentiate along the double-positive (DP) pathway. In the human postnatal thymus (PNT), DN, DP and single-positive (SP) TCRγδ(+) populations are present. Here, the precursor-progeny relationship of the various PNT TCRγδ(+) populations was studied and the role of the DP TCRγδ(+) population during T-cell differentiation was elucidated. We demonstrate that human TCRγδ(+) cells differentiate along two pathways downstream from an immature CD1(+) DN TCRγδ(+) precursor: a Notch-independent DN pathway generating mature DN and CD8αα SP TCRγδ(+) cells, and a Notch-dependent, highly proliferative DP pathway generating immature CD4 SP and subsequently DP TCRγδ(+) populations. DP TCRγδ(+) cells are actively rearranging the TCRα locus, and differentiate to TCR(-) DP cells, to CD8αβ SP TCRγδ(+) cells and to TCRαβ(+) cells. Finally, we show that the γδ subset of T-cell acute lymphoblastic leukemias (T-ALL) consists mainly of CD4 SP or DP phenotypes carrying significantly more activating Notch mutations than DN T-ALL. The latter suggests that activating Notch mutations in TCRγδ(+) thymocytes induce proliferation and differentiation along the DP pathway in vivo.
MeSH term(s)	Base Sequence ; CD4 Antigens/immunology ; CD8 Antigens/immunology ; Cell Differentiation ; Cell Proliferation ; Coculture Techniques ; DNA Primers ; Humans ; Real-Time Polymerase Chain Reaction ; Receptors, Antigen, T-Cell, gamma-delta/immunology ; Receptors, Notch/physiology ; Thymocytes/cytology ; Thymocytes/immunology
Chemical Substances	CD4 Antigens ; CD8 Antigens ; DNA Primers ; Receptors, Antigen, T-Cell, gamma-delta ; Receptors, Notch
Language	English
Publishing date	2012-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	807030-1
ISSN	1476-5551 ; 0887-6924
ISSN (online)	1476-5551
ISSN	0887-6924
DOI	10.1038/leu.2011.324
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2295: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: In vitro generation of mature, naive antigen-specific CD8(+) T cells with a single T-cell receptor by agonist selection.

Snauwaert, S / Verstichel, G / Bonte, S / Goetgeluk, G / Vanhee, S / Van Caeneghem, Y / De Mulder, K / Heirman, C / Stauss, H / Heemskerk, M H M / Taghon, T / Leclercq, G / Plum, J / Langerak, A W / Thielemans, K / Kerre, T / Vandekerckhove, B

Leukemia

2013 Volume 28, Issue 4, Page(s) 830–841

Abstract: Peripheral blood T cells transduced with a tumor-specific T-cell receptor (TCR) face problems of auto-reactivity and lack of efficacy caused by cross-pairing of exogenous and endogenous TCR chains, as well as short term in vivo survival due to activation ...

Abstract	Peripheral blood T cells transduced with a tumor-specific T-cell receptor (TCR) face problems of auto-reactivity and lack of efficacy caused by cross-pairing of exogenous and endogenous TCR chains, as well as short term in vivo survival due to activation and growth factor-induced differentiation. We here studied an alternative strategy for the efficient generation of naive CD8(+) T cells with a single TCR. TCR-transduced human postnatal thymus-derived and adult mobilized blood-derived hematopoietic progenitor cells (HPCs) were differentiated to CD4(+)CD8(+) double-positive T cells using OP9-Delta-like 1 (OP9-DL1) cultures. Addition of the agonist peptide induced double positive cells to cross-present the peptide, leading, in the absence of co-stimulation, to cell cycle arrest and differentiation into mature CD8(+) T cells. Comprehensive phenotypic, molecular and functional analysis revealed the generation of naive and resting CD8(+) T cells through a process similar to thymic positive selection. These mature T cells show a near complete inhibition of endogenous TCRA and TCRB rearrangements and express high levels of the introduced multimer-reactive TCR. Upon activation, specific cytokine production and efficient killing of tumor cells were induced. Using this strategy, large numbers of high-avidity tumor-specific naive T cells can be generated from readily available HPCs without TCR chain cross-pairing.
MeSH term(s)	Adult ; CD8-Positive T-Lymphocytes/immunology ; Cell Differentiation ; Cell Line, Tumor ; Child ; Child, Preschool ; Gene Rearrangement, T-Lymphocyte ; Humans ; Immunotherapy, Adoptive ; Infant ; Infant, Newborn ; Receptors, Antigen, T-Cell/agonists ; Receptors, Antigen, T-Cell/physiology
Chemical Substances	Receptors, Antigen, T-Cell
Language	English
Publishing date	2013-10-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	807030-1
ISSN	1476-5551 ; 0887-6924
ISSN (online)	1476-5551
ISSN	0887-6924
DOI	10.1038/leu.2013.285
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2295: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The checkpoint for agonist selection precedes conventional selection in human thymus.

Verstichel, Greet / Vermijlen, David / Martens, Liesbet / Goetgeluk, Glenn / Brouwer, Margreet / Thiault, Nicolas / Van Caeneghem, Yasmine / De Munter, Stijn / Weening, Karin / Bonte, Sarah / Leclercq, Georges / Taghon, Tom / Kerre, Tessa / Saeys, Yvan / Van Dorpe, Jo / Cheroutre, Hilde / Vandekerckhove, Bart

Science immunology

2017 Volume 2, Issue 8

Abstract: The thymus plays a central role in self-tolerance, partly by eliminating precursors with a T cell receptor (TCR) that binds strongly to self-antigens. However, the generation of self-agonist-selected lineages also relies on strong TCR signaling. How ... ...

Abstract	The thymus plays a central role in self-tolerance, partly by eliminating precursors with a T cell receptor (TCR) that binds strongly to self-antigens. However, the generation of self-agonist-selected lineages also relies on strong TCR signaling. How thymocytes discriminate between these opposite outcomes remains elusive. Here, we identified a human agonist-selected PD-1
Language	English
Publishing date	2017-02-24
Publishing country	United States
Document type	Journal Article
ISSN	2470-9468
ISSN (online)	2470-9468
DOI	10.1126/sciimmunol.aah4232
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: In vitro human embryonic stem cell hematopoiesis mimics MYB-independent yolk sac hematopoiesis.

Vanhee, Stijn / De Mulder, Katrien / Van Caeneghem, Yasmine / Verstichel, Greet / Van Roy, Nadine / Menten, Björn / Velghe, Imke / Philippé, Jan / De Bleser, Dominique / Lambrecht, Bart N / Taghon, Tom / Leclercq, Georges / Kerre, Tessa / Vandekerckhove, Bart

Haematologica

2015 Volume 100, Issue 2, Page(s) 157–166

Abstract: Although hematopoietic precursor activity can be generated in vitro from human embryonic stem cells, there is no solid evidence for the appearance of multipotent, self-renewing and transplantable hematopoietic stem cells. This could be due to short half- ... ...

Abstract	Although hematopoietic precursor activity can be generated in vitro from human embryonic stem cells, there is no solid evidence for the appearance of multipotent, self-renewing and transplantable hematopoietic stem cells. This could be due to short half-life of hematopoietic stem cells in culture or, alternatively, human embryonic stem cell-initiated hematopoiesis may be hematopoietic stem cell-independent, similar to yolk sac hematopoiesis, generating multipotent progenitors with limited expansion capacity. Since a MYB was reported to be an excellent marker for hematopoietic stem cell-dependent hematopoiesis, we generated a MYB-eGFP reporter human embryonic stem cell line to study formation of hematopoietic progenitor cells in vitro. We found CD34(+) hemogenic endothelial cells rounding up and developing into CD43(+) hematopoietic cells without expression of MYB-eGFP. MYB-eGFP(+) cells appeared relatively late in embryoid body cultures as CD34(+)CD43(+)CD45(-/lo) cells. These MYB-eGFP(+) cells were CD33 positive, proliferated in IL-3 containing media and hematopoietic differentiation was restricted to the granulocytic lineage. In agreement with data obtained on murine Myb(-/-) embryonic stem cells, bright eGFP expression was observed in a subpopulation of cells, during directed myeloid differentiation, which again belonged to the granulocytic lineage. In contrast, CD14(+) macrophage cells were consistently eGFP(-) and were derived from eGFP-precursors only. In summary, no evidence was obtained for in vitro generation of MYB(+) hematopoietic stem cells during embryoid body cultures. The observed MYB expression appeared late in culture and was confined to the granulocytic lineage.
MeSH term(s)	Cell Differentiation ; Cell Lineage ; Cells, Cultured ; Embryoid Bodies ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Endothelium, Vascular/cytology ; Endothelium, Vascular/metabolism ; Flow Cytometry ; Granulocytes/cytology ; Granulocytes/metabolism ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Hematopoiesis/physiology ; Hematopoietic Stem Cells/cytology ; Hematopoietic Stem Cells/metabolism ; Humans ; In Vitro Techniques ; Macrophages/cytology ; Macrophages/metabolism ; Proto-Oncogene Proteins c-myb/genetics ; Proto-Oncogene Proteins c-myb/metabolism ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Transgenes/physiology ; Yolk Sac/cytology ; Yolk Sac/metabolism
Chemical Substances	Proto-Oncogene Proteins c-myb ; RNA, Messenger ; enhanced green fluorescent protein ; Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2015-02
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2333-4
ISSN	1592-8721 ; 0017-6567 ; 0390-6078
ISSN (online)	1592-8721
ISSN	0017-6567 ; 0390-6078
DOI	10.3324/haematol.2014.112144
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

In stock of ZB MED Cologne/Königswinter

Uh III Zs.76: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: RHAMM/HMMR (CD168) is not an ideal target antigen for immunotherapy of acute myeloid leukemia.

Snauwaert, Sylvia / Vanhee, Stijn / Goetgeluk, Glenn / Verstichel, Greet / Van Caeneghem, Yasmine / Velghe, Imke / Philippé, Jan / Berneman, Zwi N / Plum, Jean / Taghon, Tom / Leclercq, Georges / Thielemans, Kris / Kerre, Tessa / Vandekerckhove, Bart

Haematologica

2012 Volume 97, Issue 10, Page(s) 1539–1547

Abstract: Background: Criteria for good candidate antigens for immunotherapy of acute myeloid leukemia are high expression on leukemic stem cells in the majority of patients with acute myeloid leukemia and low or no expression in vital tissues. It was shown in ... ...

Abstract	Background: Criteria for good candidate antigens for immunotherapy of acute myeloid leukemia are high expression on leukemic stem cells in the majority of patients with acute myeloid leukemia and low or no expression in vital tissues. It was shown in vaccination trials that Receptor for Hyaluronic Acid Mediated Motility (RHAMM/HMMR) generates cellular immune responses in patients with acute myeloid leukemia and that these responses correlate with clinical benefit. It is not clear however whether this response actually targets the leukemic stem cell, especially since it was reported that RHAMM is expressed maximally during the G2/M phase of the cell cycle. In addition, tumor specificity of RHAMM expression remains relatively unexplored. Design and methods: Blood, leukapheresis and bone marrow samples were collected from both acute myeloid leukemia patients and healthy controls. RHAMM expression was assessed at protein and mRNA levels on various sorted populations, either fresh or after manipulation. Results: High levels of RHAMM were expressed by CD34(+)CD38(+) and CD34(-) acute myeloid leukemia blasts. However, only baseline expression of RHAMM was measured in CD34(+)CD38(-) leukemic stem cells, and was not different from that in CD34(+)CD38(-) hematopoietic stem cells from healthy controls. RHAMM was significantly up-regulated in CD34(+) cells from healthy donors during in vitro expansion and during in vivo engraftment. Finally, we demonstrated an explicit increase in the expression level of RHAMM after in vitro activation of T cells. Conclusions: RHAMM does not fulfill the criteria of an ideal target antigen for immunotherapy of acute myeloid leukemia. RHAMM expression in leukemic stem cells does not differ significantly from the expression in hematopoietic stem cells from healthy controls. RHAMM expression in proliferating CD34+ cells of healthy donors and activated T cells further compromises RHAMM-specific T-cell-mediated immunotherapy.
MeSH term(s)	Adult ; Aged ; Animals ; Antigens, CD34/metabolism ; Cell Cycle ; Cell Line, Tumor ; Disease Models, Animal ; Extracellular Matrix Proteins/genetics ; Extracellular Matrix Proteins/immunology ; Extracellular Matrix Proteins/metabolism ; Female ; Gene Expression Regulation, Leukemic ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells/immunology ; Hematopoietic Stem Cells/metabolism ; Humans ; Hyaluronan Receptors/genetics ; Hyaluronan Receptors/immunology ; Hyaluronan Receptors/metabolism ; Immunophenotyping ; Immunotherapy ; Leukemia, Myeloid, Acute/immunology ; Leukemia, Myeloid, Acute/therapy ; Lymphocyte Activation/immunology ; Male ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Middle Aged ; Neoplastic Stem Cells/immunology ; Neoplastic Stem Cells/metabolism ; T-Lymphocytes/immunology ; T-Lymphocytes/metabolism ; Young Adult
Chemical Substances	Antigens, CD34 ; Extracellular Matrix Proteins ; Hyaluronan Receptors ; hyaluronan-mediated motility receptor
Language	English
Publishing date	2012-04-24
Publishing country	Italy
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2333-4
ISSN	1592-8721 ; 0017-6567 ; 0390-6078
ISSN (online)	1592-8721
ISSN	0017-6567 ; 0390-6078
DOI	10.3324/haematol.2012.065581
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uh III Zs.76: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Functionally mature CD4 and CD8 TCRalphabeta cells are generated in OP9-DL1 cultures from human CD34+ hematopoietic cells.

Van Coppernolle, Stefanie / Verstichel, Greet / Timmermans, Frank / Velghe, Imke / Vermijlen, David / De Smedt, Magda / Leclercq, Georges / Plum, Jean / Taghon, Tom / Vandekerckhove, Bart / Kerre, Tessa

Journal of immunology (Baltimore, Md. : 1950)

2009 Volume 183, Issue 8, Page(s) 4859–4870

Abstract: Human CD34(+) hematopoietic precursor cells cultured on delta-like ligand 1 expressing OP9 (OP9-DL1) stromal cells differentiate to T lineage cells. The nature of the T cells generated in these cultures has not been studied in detail. Since these ... ...

Abstract	Human CD34(+) hematopoietic precursor cells cultured on delta-like ligand 1 expressing OP9 (OP9-DL1) stromal cells differentiate to T lineage cells. The nature of the T cells generated in these cultures has not been studied in detail. Since these cultures do not contain thymic epithelial cells which are the main cell type mediating positive selection in vivo, generation of conventional helper CD4(+) and cytotoxic CD8(+) TCRalphabeta cells is not expected. Phenotypically mature CD27(+)CD1(-) TCRgammadelta as well as TCRalphabeta cells were generated in OP9-DL1 cultures. CD8 and few mature CD4 single-positive TCRalphabeta cells were observed. Mature CD8 single-positive cells consisted of two subpopulations: one expressing mainly CD8alphabeta and one expressing CD8alphaalpha dimers. TCRalphabeta CD8alphaalpha and TCRgammadelta cells both expressed the IL2Rbeta receptor constitutively and proliferated on IL-15, a characteristic of unconventional T cells. CD8alphabeta(+) and CD4(+) TCRalphabeta cells were unresponsive to IL-15, but could be expanded upon TCR stimulation as mature CD8alphabeta(+) and CD4(+) T cells. These T cells had the characteristics of conventional T cells: CD4(+) cells expressed ThPOK, CD40L, and high levels of IL-2 and IL-4; CD8(+) cells expressed Eomes, Runx3, and high levels of granzyme, perforin, and IFN-gamma. Induction of murine or human MHC class I expression on OP9-DL1 cells had no influence on the differentiation of mature CD8(+) cells. Similarly, the presence of dendritic cells was not required for the generation of mature CD4(+) or CD8(+) T cells. These data suggest that positive selection of these cells is induced by interaction between T precursor cells.
MeSH term(s)	CD4-Positive T-Lymphocytes/drug effects ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; CD40 Ligand/immunology ; CD40 Ligand/metabolism ; CD8-Positive T-Lymphocytes/drug effects ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Cell Differentiation/immunology ; Cell Line ; Coculture Techniques ; DNA-Binding Proteins/immunology ; DNA-Binding Proteins/metabolism ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Hematopoietic Stem Cells/drug effects ; Hematopoietic Stem Cells/immunology ; Hematopoietic Stem Cells/metabolism ; Histocompatibility Antigens Class I/immunology ; Histocompatibility Antigens Class I/metabolism ; Humans ; Interleukin-15/pharmacology ; Interleukin-2/immunology ; Interleukin-2/metabolism ; Interleukin-4/immunology ; Interleukin-4/metabolism ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Receptors, Antigen, T-Cell, alpha-beta/metabolism ; Receptors, Interleukin-2/immunology ; Receptors, Interleukin-2/metabolism ; Transcription Factors/immunology ; Transcription Factors/metabolism
Chemical Substances	DNA-Binding Proteins ; Histocompatibility Antigens Class I ; IL2 protein, human ; IL4 protein, human ; Interleukin-15 ; Interleukin-2 ; Receptors, Antigen, T-Cell, alpha-beta ; Receptors, Interleukin-2 ; Transcription Factors ; ZBTB7B protein, human ; CD40 Ligand (147205-72-9) ; Interleukin-4 (207137-56-2)
Language	English
Publishing date	2009-10-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.0900714
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.37: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 28: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito