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Article ; Online: Ocular Tuberculosis in Pediatrics: A Case Report.

Vieira, Paula Manuel / Zilhão, Carla / Miranda, Vasco

2023 Volume 36, Issue 10, Page(s) 683–686

Abstract: A 17-year-old male was taken to the emergency department for decreased left visual acuity and floaters beginning that same day. There was a history of exposure to pulmonary tuberculosis five years before (mother as index case) followed by a four-month ... ...

Abstract	A 17-year-old male was taken to the emergency department for decreased left visual acuity and floaters beginning that same day. There was a history of exposure to pulmonary tuberculosis five years before (mother as index case) followed by a four-month period of isoniazid prophylaxis. The ophthalmic examination showed posterior and intermediate uveitis in the left eye. Laboratory tests were normal; IgG for herpes simplex 1 was positive and both the varicella-zoster virus and remaining serologic tests were negative. Chest radiography was normal. Two weeks later, an epiretinal membrane with risk of tractional retinal detachment was observed. The Mantoux tuberculin skin test showed an induration of 15 mm and the IGRA test was positive. Sputum and vitreous humor samples were collected. Quadruple therapy and prednisolone were started. Ten days later, a posterior vitreous detachment with underlying vitreous hematoma was observed. Posterior vitrectomy and peripheral endolaser were performed without complications. One month later, the microbiological results became available, with the identification of Mycobacterium tuberculosis. Corticosteroids were weaned progressively. Antituberculous drugs were maintained for six months. The patient made a full recovery.
MeSH term(s)	Male ; Humans ; Child ; Adolescent ; Tuberculosis, Ocular/diagnosis ; Tuberculosis, Ocular/drug therapy ; Tuberculosis, Ocular/surgery ; Vitreous Body/microbiology ; Vitreous Body/surgery ; Vitrectomy ; Eye Diseases/surgery ; Mycobacterium tuberculosis
Language	English
Publishing date	2023-04-19
Publishing country	Portugal
Document type	Case Reports ; Journal Article
ZDB-ID	603078-6
ISSN	1646-0758 ; 0870-399X
ISSN (online)	1646-0758
ISSN	0870-399X
DOI	10.20344/amp.19245
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Article ; Online: Vulvar necrotic lesion. An uncommon diagnosis.

Vieira, Paula Manuel / da Silva Cardoso, Juliana / Teixeira, Carla / Guerra, Isabel Couto

Anales de pediatria

2024

Language	English
Publishing date	2024-04-05
Publishing country	Spain
Document type	Journal Article
ZDB-ID	2830901-7
ISSN	2341-2879 ; 2341-2879
ISSN (online)	2341-2879
ISSN	2341-2879
DOI	10.1016/j.anpede.2024.03.020
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Article: Gold-Coated Superparamagnetic Iron Oxide Nanoparticles Functionalized to EGF and Ce6 Complexes for Breast Cancer Diagnoses and Therapy.

Cândido, Marcela / Vieira, Paula / Campos, Andrea / Soares, Cristina / Raniero, Leandro

Pharmaceutics

2022 Volume 15, Issue 1

Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) have some limitations in the physiological environment, however, a modification on their surface, such as a core-shell structure with gold (SPIONs@Au), can enhance their applicability. In this study, ... ...

Abstract	Superparamagnetic iron oxide nanoparticles (SPIONs) have some limitations in the physiological environment, however, a modification on their surface, such as a core-shell structure with gold (SPIONs@Au), can enhance their applicability. In this study, SPIONs were synthesized by the chemical coprecipitation method, stabilized by sodium citrate, and followed by the gold-coating process. SPIONs@Au were functionalized with EGF-α-lipoic acid and chlorin e6 (Ce6)-cysteamine complexes, composing a Theranostic Nanoprobe (TP). The outcomes showed that the SPIONs@Au had changed in color to red and had an absorption band centered at 530 nm. The coating was verified in the TEM micrographs in bright and dark fields by EDS mapping, which indicated the presence of Au and Fe. The Ce6-cysteamine complex had a resonant band at 670 nm that enabled the diagnosis of biological samples using fluorescence analysis. In the measure of TNBC cell uptake, the maximum value of TP fluorescence intensity was obtained within 4 h of internalization. At 2 h, the incorporation of the TP in the cytoplasm as well as in the nuclei was observed, suggesting that it could be employed as a diagnostic marker. The PTT results showed significant percentages of apoptosis in the TNBC cell line, which confirms the efficacy of the TP.
Language	English
Publishing date	2022-12-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15010100
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Article ; Online: Medullary Compression by a Cervical Osteochondroma in a Patient with Multiple Hereditary Exostoses: A Case Report.

Lixa, João / Pinho, André / Vieira, Paula / Vieira da Silva, Nuno / Silva, Miguel Relvas / Marques, Ana / Veludo, Vitorino

JBJS case connector

2024 Volume 14, Issue 1

Abstract: Case: A 19-year-old man with Multiple Hereditary Exostoses presented with cervical pain without neurological symptoms and/or signs. Magnetic resonance revealed a large C2 osteochondroma, occupying a part of the medullary canal. He was submitted to an en ...

Abstract	Case: A 19-year-old man with Multiple Hereditary Exostoses presented with cervical pain without neurological symptoms and/or signs. Magnetic resonance revealed a large C2 osteochondroma, occupying a part of the medullary canal. He was submitted to an en bloc resection with hemilaminectomy without fusion. At the 1-year follow-up, he presented resolution of pain and no neurological symptoms or signs, without cervical instability or radiological signs of disease recurrence. Conclusion: Cervical osteochondroma is usually asymptomatic. Neurological compression and differentiation to chondrosarcoma are the main concerns. Surgical excision allows the local cure of the disease and is usually performed without fusion.
MeSH term(s)	Humans ; Male ; Young Adult ; Exostoses, Multiple Hereditary/complications ; Exostoses, Multiple Hereditary/diagnostic imaging ; Exostoses, Multiple Hereditary/surgery ; Neoplasm Recurrence, Local ; Osteochondroma/complications ; Osteochondroma/diagnostic imaging ; Osteochondroma/surgery ; Spinal Neoplasms/complications ; Spinal Neoplasms/diagnostic imaging ; Spinal Neoplasms/surgery ; Treatment Outcome
Language	English
Publishing date	2024-01-11
Publishing country	United States
Document type	Case Reports ; Journal Article
ISSN	2160-3251
ISSN (online)	2160-3251
DOI	e23.00429
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Article ; Online: Ulnar-Sided Upper Extremity Traumatic Wounds: What Should We Expect to Find?

Costa, João Q / Leite, Maria J / Relvas, Miguel / Vieira, Paula / Negrão, Pedro / Vidinha, Vitor

The journal of hand surgery Asian-Pacific volume

2023 Volume 28, Issue 4, Page(s) 435–440

Abstract: Background: ...

Abstract	Background:
Language	English
Publishing date	2023-09-25
Publishing country	Singapore
Document type	Journal Article
ZDB-ID	2848651-1
ISSN	2424-8363 ; 2424-8355
ISSN (online)	2424-8363
ISSN	2424-8355
DOI	10.1142/S2424835523500480
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Article ; Online: Quality of life in adolescents with narcolepsy type 1- a transversal study in a tertiary hospital.

Salazar, Luís / Vieira, Paula Manuel / Cascais, Inês / Figueiroa, Sónia / Rios, Marta

Sleep medicine

2023 Volume 113, Page(s) 215–219

Abstract: Purpose: - Narcolepsy type 1 (NT1) is a rare chronic sleep disorder, usually arising by adolescence that negatively impacts quality of life. It is characterized by excessive daytime sleepiness (EDS), cataplexy, hypnagogic/hypnopompic hallucinations, ... ...

Abstract	Purpose: - Narcolepsy type 1 (NT1) is a rare chronic sleep disorder, usually arising by adolescence that negatively impacts quality of life. It is characterized by excessive daytime sleepiness (EDS), cataplexy, hypnagogic/hypnopompic hallucinations, sleep paralysis and sleep fragmentation. The goals of this work were to characterize NT1 adolescents regarding sleep characteristics, health-related quality of life (HRQoL) and future life perspectives and later to compare this group with a control group of healthy adolescents (HA). Methods: - Transversal descriptive/analytical study including NT1 patients followed in a sleep center of a tertiary hospital and 23 HA. Data were collected through an online survey, fulfilled by the participants, including four sections: demographics; questionnaire evaluating sleep and EDS; questionnaire evaluating HRQoL; inquiry regarding future perspectives. An extra section for the NT1 group only, comprising questions about the characterization of narcolepsy, was included. Results: 22 NT1 adolescents were included, with a median age of 15.0 years-old. Beyond EDS, all had presented cataplexy - 19 still reported it. Twenty patients took psychostimulants regularly for EDS, while 13 patients took venlafaxine or fluoxetine for cataplexy. Nineteen adolescents took regular naps and 19 maintained psychological appointments. Self-reported sleep quality was similar between groups (p = 0.112). EDS was identified in seven NT1 patients and none in the control group. HRQOL was significantly lower in NT1 patients only for the physical well-being domain (p = 0.001). Regarding future perspectives, results were similar, except for a lower probability of getting a driver's license in NT1 patients, despite no statistical significance (p = 0.104). Discussion: Daytime sleepiness is difficult to control in NT1, despite specialized treatment. HRQoL was similar between groups in all domains except for the physical well-being. Despite good adherence to pharmacological and non-pharmacological treatments (namely psychological therapy) that account for these good results, the physical well-being domain is difficult to manage.
MeSH term(s)	Humans ; Adolescent ; Cataplexy/drug therapy ; Quality of Life ; Tertiary Care Centers ; Narcolepsy/drug therapy ; Disorders of Excessive Somnolence
Language	English
Publishing date	2023-11-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2012041-2
ISSN	1878-5506 ; 1389-9457
ISSN (online)	1878-5506
ISSN	1389-9457
DOI	10.1016/j.sleep.2023.11.030
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Zs.A 5315: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma.

Vieira, Paula / Jesus, Viviane / Cândido, Marcela Aparecida / Pacheco-Soares, Cristina / Castilho, Maiara / Raniero, Leandro

Photodiagnosis and photodynamic therapy

2022 Volume 39, Page(s) 102997

Abstract: Background: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor ... ...

Abstract	Background: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nanomaterials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the importance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker molecule METHODS: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitro RESULTS: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression properties CONCLUSION: The findings point to ideal properties for detecting EGFR receptor overexpressed cells.
MeSH term(s)	Breast Neoplasms ; Cell Line, Tumor ; Epidermal Growth Factor/metabolism ; ErbB Receptors ; Female ; Glioblastoma/metabolism ; Gold ; Humans ; Metal Nanoparticles ; Photochemotherapy/methods ; Triple Negative Breast Neoplasms
Chemical Substances	Epidermal Growth Factor (62229-50-9) ; Gold (7440-57-5) ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1)
Language	English
Publishing date	2022-07-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2022.102997
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Article ; Online: Transitioning from static to suspension culture system for large-scale production of xeno-free extracellular vesicles derived from mesenchymal stromal cells.

Dos Santos, Natália Cristine Dias / Bruzadelle-Vieira, Paula / de Cássia Noronha, Nádia / Mizukami-Martins, Amanda / Orellana, Maristela Delgado / Bentley, Maria Vitória L B / Covas, Dimas Tadeu / Swiech, Kamilla / Malmegrim, Kelen Cristina Ribeiro

Biotechnology progress

2024 , Page(s) e3419

Abstract: Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) have shown increasing therapeutic potential in the last years. However, large production of EV is required for therapeutic purposes. Thereby, scaling up MSC cultivation in ... ...

Abstract	Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) have shown increasing therapeutic potential in the last years. However, large production of EV is required for therapeutic purposes. Thereby, scaling up MSC cultivation in bioreactors is essential to allow culture parameters monitoring. In this study, we reported the establishment of a scalable bioprocess to produce MSC-EV in suspension cultures using spinner flasks and human collagen-coated microcarriers (3D culture system). We compared the EV production in this 3D culture system with the standard static culture using T-flasks (2D culture system). The EV produced in both systems were characterized and quantify by western blotting and nanoparticle tracking analysis. The presence of the typical protein markers CD9, CD63, and CD81 was confirmed by western blotting analyses for EV produced in both culture systems. The cell fold-increase was 5.7-fold for the 3D culture system and 4.6-fold for the 2D culture system, signifying a fold-change of 1.2 (calculated as the ratio of fold-increase 3D to fold-increase 2D). Furthermore, it should be noted that the total cell production in the spinner flask cultures was 4.8 times higher than that in T-flask cultures. The total cell production in the spinner flask cultures was 5.2-fold higher than that in T-flask cultures. While the EV specific production (particles/cell) in T-flask cultures (4.40 ± 1.21 × 10
Language	English
Publishing date	2024-01-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	165657-0
ISSN	1520-6033 ; 8756-7938
ISSN (online)	1520-6033
ISSN	8756-7938
DOI	10.1002/btpr.3419
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Article ; Online: Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer.

Castilho, Maiara L / Jesus, Viviane P S / Vieira, Paula F A / Hewitt, Kevin C / Raniero, L

Photodiagnosis and photodynamic therapy

2021 Volume 33, Page(s) 102186

Abstract: To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold ... ...

Abstract	To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 μg/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency.
MeSH term(s)	Animals ; Cell Line, Tumor ; Epidermal Growth Factor ; Gold ; Humans ; Metal Nanoparticles ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nanomedicine ; Nanoparticles ; Photochemotherapy/methods ; Photosensitizing Agents/pharmacology ; Photosensitizing Agents/therapeutic use ; Porphyrins/pharmacology ; Triple Negative Breast Neoplasms/drug therapy
Chemical Substances	Photosensitizing Agents ; Porphyrins ; phytochlorin (5S2CCF3T1Z) ; Epidermal Growth Factor (62229-50-9) ; Gold (7440-57-5)
Language	English
Publishing date	2021-01-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2149918-4
ISSN	1873-1597 ; 1572-1000
ISSN (online)	1873-1597
ISSN	1572-1000
DOI	10.1016/j.pdpdt.2021.102186
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Article ; Online: Novel Fe

Valverde, Thalita Marcolan / Dos Santos, Viviane Martins Rebello / Viana, Pedro Igor Macário / Costa, Guilherme Mattos Jardim / de Goes, Alfredo Miranda / Sousa, Lucas Resende Dutra / Xavier, Viviane Flores / Vieira, Paula Melo de Abreu / de Lima Silva, Daniel / Domingues, Rosana Zacarias / Ferreira, José Maria da Fonte / Andrade, Ângela Leão

International journal of molecular sciences

2024 Volume 25, Issue 8

Abstract: Immune response to biomaterials, which is intimately related to their surface properties, can produce chronic inflammation and fibrosis, leading to implant failure. This study investigated the development of magnetic nanoparticles coated with silica and ... ...

Abstract	Immune response to biomaterials, which is intimately related to their surface properties, can produce chronic inflammation and fibrosis, leading to implant failure. This study investigated the development of magnetic nanoparticles coated with silica and incorporating the anti-inflammatory drug naproxen, aimed at multifunctional biomedical applications. The synthesized nanoparticles were characterized using various techniques that confirmed the presence of magnetite and the formation of a silica-rich bioactive glass (BG) layer. In vitro studies demonstrated that the nanoparticles exhibited bioactive properties, forming an apatite surface layer when immersed in simulated body fluid, and biocompatibility with bone cells, with good viability and alkaline phosphatase activity. Naproxen, either free or encapsulated, reduced nitric oxide production, an inflammatory marker, while the BG coating alone did not show anti-inflammatory effects in this study. Overall, the magnetic nanoparticles coated with BG and naproxen showed promise for biomedical applications, especially anti-inflammatory activity in macrophages and in the bone field, due to their biocompatibility, bioactivity, and osteogenic potential.
MeSH term(s)	Naproxen/pharmacology ; Naproxen/chemistry ; Glass/chemistry ; Coated Materials, Biocompatible/chemistry ; Coated Materials, Biocompatible/pharmacology ; Magnetite Nanoparticles/chemistry ; Animals ; Mice ; Humans ; Nitric Oxide/metabolism ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Silicon Dioxide/chemistry ; Cell Survival/drug effects ; RAW 264.7 Cells ; Osteogenesis/drug effects
Chemical Substances	Naproxen (57Y76R9ATQ) ; Coated Materials, Biocompatible ; Magnetite Nanoparticles ; Nitric Oxide (31C4KY9ESH) ; Biocompatible Materials ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2024-04-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms25084270
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