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  1. Article ; Online: ASP-2/Trans-sialidase chimeric protein induces robust protective immunity in experimental models of Chagas' disease.

    Castro, Julia T / Brito, Rory / Hojo-Souza, Natalia S / Azevedo, Bárbara / Salazar, Natalia / Ferreira, Camila P / Junqueira, Caroline / Fernandes, Ana Paula / Vasconcellos, Ronnie / Cardoso, Jamille M / Aguiar-Soares, Rodrigo D O / Vieira, Paula M A / Carneiro, Cláudia M / Valiate, Bruno / Toledo, Cristiane / Salazar, Andres M / Caballero, Otávia / Lannes-Vieira, Joseli / Teixeira, Santuza R /
    Reis, Alexandre B / Gazzinelli, Ricardo T

    NPJ vaccines

    2023  Volume 8, Issue 1, Page(s) 81

    Abstract: Immunization with the Amastigote Surface Protein-2 (ASP-2) and Trans-sialidase (TS) antigens either in the form of recombinant protein, encoded in plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages of Trypanosoma ... ...

    Abstract Immunization with the Amastigote Surface Protein-2 (ASP-2) and Trans-sialidase (TS) antigens either in the form of recombinant protein, encoded in plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages of Trypanosoma cruzi. Herein we generated a chimeric protein containing the most immunogenic regions for T and B cells from TS and ASP-2 (TRASP) and evaluated its immunogenicity in comparison with our standard protocol of heterologous prime-boost using plasmids and hAd5. Mice immunized with TRASP protein associated to Poly-ICLC (Hiltonol) were highly resistant to challenge with T. cruzi, showing a large decrease in tissue parasitism, parasitemia and no lethality. This protection lasted for at least 3 months after the last boost of immunization, being equivalent to the protection induced by DNA/hAd5 protocol. TRASP induced high levels of T. cruzi-specific antibodies and IFNγ-producing T cells and protection was primarily mediated by CD8
    Language English
    Publishing date 2023-05-31
    Publishing country England
    Document type Journal Article
    ISSN 2059-0105
    ISSN (online) 2059-0105
    DOI 10.1038/s41541-023-00676-0
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  2. Article ; Online: Protective Effects of Quercetin on Livers from Mice Exposed to Long-Term Cigarette Smoke.

    Machado-Junior, Pedro A / Araújo, Natália P S / Souza, Ana B F / Castro, Thalles F / Oliveira, Michel / Costa, Guilherme P / Matos, Natália A / Vieira, Paula M A / Talvani, André / Bezerra, Frank S / Cangussú, Sílvia D

    BioMed research international

    2020  Volume 2020, Page(s) 2196207

    Abstract: Cigarette smoke is highly toxic, and it can promote increased production of reactive species and inflammatory response and leads to liver diseases. Quercetin is a flavonoid that displays antioxidant and anti-inflammatory activities in liver diseases. ... ...

    Abstract Cigarette smoke is highly toxic, and it can promote increased production of reactive species and inflammatory response and leads to liver diseases. Quercetin is a flavonoid that displays antioxidant and anti-inflammatory activities in liver diseases. This study aimed at evaluating the protective effects of quercetin on livers from mice exposed to long-term cigarette smoke exposure. Male C57BL/6 mice were divided into five groups: control (CG), vehicle (VG), quercetin (QG), cigarette smoke (CSG), quercetin, and cigarette smoke (QCSG). CSG and QCSG were exposed to cigarette smoke for sixty consecutive days; at the end of the exposures, all animals were euthanized. Mice that received quercetin daily and were exposed to cigarette smoke showed a reduced influx of inflammatory cells, oxidative stress, inflammatory reaction, and histopathological changes in the liver, compared to CSG. These results suggest that quercetin may be an effective adjuvant for treating damage to the liver due to cigarette smoke exposure.
    MeSH term(s) Animals ; Antioxidants/pharmacology ; Biomarkers/metabolism ; Body Weight ; Cigarette Smoking/adverse effects ; Inflammation Mediators/metabolism ; Liver/drug effects ; Liver/pathology ; Liver/physiopathology ; Liver Function Tests ; Male ; Mice, Inbred C57BL ; Oxidative Stress/drug effects ; Protective Agents/pharmacology ; Quercetin/pharmacology
    Chemical Substances Antioxidants ; Biomarkers ; Inflammation Mediators ; Protective Agents ; Quercetin (9IKM0I5T1E)
    Language English
    Publishing date 2020-11-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2698540-8
    ISSN 2314-6141 ; 2314-6133
    ISSN (online) 2314-6141
    ISSN 2314-6133
    DOI 10.1155/2020/2196207
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Preparation of glass-ionomer cement containing ethanolic Brazilian pepper extract (Schinus terebinthifolius Raddi) fruits: chemical and biological assays.

    Pinto, Isabelle C / Seibert, Janaína B / Pinto, Luciano S / Santos, Vagner R / de Sousa, Rafaela F / Sousa, Lucas R D / Amparo, Tatiane R / Dos Santos, Viviane M R / do Nascimento, Andrea M / de Souza, Gustavo Henrique Bianco / Vasconcellos, Walisson A / Vieira, Paula M A / Andrade, Ângela L

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 22312

    Abstract: Plants may contain beneficial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic ... ...

    Abstract Plants may contain beneficial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass-ionomer cement (GIC) and the composite GIC-BPE were characterized by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and thermal analysis. The BPE compounds were identified by UPLC-QTOF-MS/MS. The release profile of flavonoids and the mechanical properties of the GIC-BPE composite were assessed. The flavonoids were released through a linear mechanism governing the diffusion for the first 48 h, as evidenced by the M
    MeSH term(s) Anacardiaceae/chemistry ; Biocompatible Materials/chemistry ; Biocompatible Materials/pharmacology ; Brazil ; Compressive Strength ; Fruit/chemistry ; Glass Ionomer Cements/chemical synthesis ; Glass Ionomer Cements/chemistry ; Glass Ionomer Cements/pharmacology ; Humans ; Materials Testing ; Microscopy, Electron, Scanning ; Mouth/chemistry ; Mouth/drug effects ; Mouth/microbiology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Spectroscopy, Fourier Transform Infrared
    Chemical Substances Biocompatible Materials ; Glass Ionomer Cements ; Plant Extracts
    Language English
    Publishing date 2020-12-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-79257-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Nanostructured Systems Improve the Antimicrobial Potential of the Essential Oil from

    Seibert, Janaína B / Viegas, Juliana S R / Almeida, Tamires C / Amparo, Tatiane R / Rodrigues, Ivanildes V / Lanza, Juliane S / Frézard, Frédéric J G / Soares, Rodrigo D O A / Teixeira, Luiz Fernando M / de Souza, Gustavo H B / Vieira, Paula M A / Barichello, José M / Dos Santos, Orlando D H

    Journal of natural products

    2019  Volume 82, Issue 12, Page(s) 3208–3220

    Abstract: The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems ... ...

    Abstract The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems containing
    MeSH term(s) Animals ; Anti-Infective Agents/chemistry ; Anti-Infective Agents/pharmacology ; Bacteria/drug effects ; Cell Line ; Cell Membrane Permeability/drug effects ; Cymbopogon/chemistry ; Drug Screening Assays, Antitumor ; Fungi/drug effects ; Gas Chromatography-Mass Spectrometry ; Hydrogen-Ion Concentration ; Mice ; Microbial Sensitivity Tests ; Microscopy, Electron, Transmission ; Nanostructures ; Oils, Volatile/chemistry ; Oils, Volatile/pharmacology ; Particle Size ; Plant Leaves/chemistry ; Quantitative Structure-Activity Relationship
    Chemical Substances Anti-Infective Agents ; Oils, Volatile
    Language English
    Publishing date 2019-12-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/acs.jnatprod.8b00870
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1144: Show issues Location:
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  5. Article: Nanostructured Systems Improve the Antimicrobial Potential of the Essential Oil from Cymbopogon densiflorus Leaves

    Seibert, Janaína B / Viegas, Juliana S. R / Almeida, Tamires C / Amparo, Tatiane R / Rodrigues, Ivanildes V / Lanza, Juliane S / Frézard, Frédéric J. G / Soares, Rodrigo D. O. A / Teixeira, Luiz Fernando M / de Souza, Gustavo H. B / Vieira, Paula M. A / Barichello, José M / dos Santos, Orlando D. H

    Journal of natural products. 2019 Dec. 09, v. 82, no. 12

    2019  

    Abstract: The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems containing Cymbopogon densiflorus leaf essential oil and to evaluate ... ...

    Abstract The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems containing Cymbopogon densiflorus leaf essential oil and to evaluate their antimicrobial activity. The essential oil was isolated by hydrodistillation from leaves and analyzed by GC-MS. The main constituents were found to be trans-p-mentha-2,8-dien-1-ol, cis-p-mentha-2,8-dien-1-ol, trans-p-mentha-1(7),8-dien-2-ol, cis-piperitol, and cis-p-mentha-1(7),8-dien-2-ol. In silico prediction analysis suggested that this oil possesses antimicrobial potential and the main mechanism of action might be the peptidoglycan glycosyltransferase inhibition. Nanoemulsions were prepared by the phase inversion method, and liposomes were made by the film hydration method. Qualitative evaluation of the antimicrobial activity was performed by the diffusion disk assay with 24 microorganisms; all of them were found to be sensitive to the essential oil. Subsequently, this property was quantified by the serial microdilution technique, where the nanoformulations demonstrated improved activity in comparison with the free oil. Bactericidal action was tested by the propidium iodide method, which revealed that free essential oil and nanoemulsion increased cytoplasmic membrane permeability, while no difference was observed between negative control and liposome. These results were confirmed by images obtained using transmission electron microscopy. This study has shown an optimization in the antimicrobial activity of C. densiflorus essential oil by a nanoemulsion and a liposomal formulation of the active substances.
    Keywords Cymbopogon ; active ingredients ; antimicrobial properties ; cell membranes ; drug development ; enzyme inhibition ; essential oils ; gas chromatography-mass spectrometry ; hydrodistillation ; leaves ; mechanism of action ; membrane permeability ; microorganisms ; nanoemulsions ; oils ; peptidoglycans ; physicochemical properties ; prediction ; propidium ; transferases ; transmission electron microscopy
    Language English
    Dates of publication 2019-1209
    Size p. 3208-3220.
    Publishing place American Chemical Society and American Society of Pharmacognosy
    Document type Article
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/acs.jnatprod.8b00870
    Database NAL-Catalogue (AGRICOLA)

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    Zs.A 1144: Show issues Location:
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  6. Article: Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope

    Rodrigues, Marcella R / Santos, Lucas M.O / Miyazaki, Carolina K / Martins, Vivian T / Ludolf, Fernanda R / Kursancew, Amanda C / Ramos, Fernanda F / Dias, Daniel S / Oliveira, Jamil S / Vieira, Paula M.A / Roatt, Bruno M / Machado de Ávila, Ricardo A / Gonçalves, Denise U / Menezes-Souza, Daniel / Coelho, Eduardo A.F / Duarte, Mariana C

    Journal of immunological methods. 2019 Aug. 06,

    2019  

    Abstract: In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study ... ...

    Abstract In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study analyzed serum samples from cutaneous (n = 20) or mucosal (n = 39) leishmaniasis patients, and from Chagas disease (CD) patients (n = 8) and non-infected patients (n = 45). For CVL diagnosis, serum samples from asymptomatic (n = 14), symptomatic (n = 71), non-infected (n = 116), and Leish-Tec®-vaccinated (n = 79) dogs were examined, as well as T. cruzi (n = 11) and Ehrlichia canis (n = 10) infected animals. An indirect ELISA method using rProhibitin showed diagnostic sensitivity and specificity values of 91.76% and 89.91%, respectively. L. infantum SLA showed 86.11% and 48.24% of specificity and sensitivity, respectively, for CVL serodiagnosis, and 98.31% and 84.91% sensitivity and specificity, respectively for TL diagnosis. L. braziliensis SLA showed 75.47% and 83.05% of specificity and sensitivity, respectively, for TL diagnosis. The synthetic peptide showed a better result in TL than in CVL diagnosis. In conclusion, preliminary results suggest that the detection of antibodies against the rProhibitin protein and the synthetic peptide improves the serodiagnosis of TL and CVL.
    Keywords B-lymphocytes ; Chagas disease ; Ehrlichia canis ; Leishmania braziliensis ; Leishmania infantum ; antibodies ; blood serum ; diagnostic sensitivity ; dogs ; enzyme-linked immunosorbent assay ; epitopes ; humans ; patients ; serodiagnosis ; synthetic peptides ; visceral leishmaniasis
    Language English
    Dates of publication 2019-0806
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2019.112641
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  7. Article ; Online: Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope.

    Rodrigues, Marcella R / Santos, Lucas M O / Miyazaki, Carolina K / Martins, Vivian T / Ludolf, Fernanda R / Kursancew, Amanda C / Ramos, Fernanda F / Dias, Daniel S / Oliveira, Jamil S / Vieira, Paula M A / Roatt, Bruno M / Machado de Ávila, Ricardo A / Gonçalves, Denise U / Menezes-Souza, Daniel / Coelho, Eduardo A F / Duarte, Mariana C

    Journal of immunological methods

    2019  Volume 474, Page(s) 112641

    Abstract: In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study ... ...

    Abstract In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study analyzed serum samples from cutaneous (n = 20) or mucosal (n = 39) leishmaniasis patients, and from Chagas disease (CD) patients (n = 8) and non-infected patients (n = 45). For CVL diagnosis, serum samples from asymptomatic (n = 14), symptomatic (n = 71), non-infected (n = 116), and Leish-Tec®-vaccinated (n = 79) dogs were examined, as well as T. cruzi (n = 11) and Ehrlichia canis (n = 10) infected animals. An indirect ELISA method using rProhibitin showed diagnostic sensitivity and specificity values of 91.76% and 89.91%, respectively. L. infantum SLA showed 86.11% and 48.24% of specificity and sensitivity, respectively, for CVL serodiagnosis, and 98.31% and 84.91% sensitivity and specificity, respectively for TL diagnosis. L. braziliensis SLA showed 75.47% and 83.05% of specificity and sensitivity, respectively, for TL diagnosis. The synthetic peptide showed a better result in TL than in CVL diagnosis. In conclusion, preliminary results suggest that the detection of antibodies against the rProhibitin protein and the synthetic peptide improves the serodiagnosis of TL and CVL.
    MeSH term(s) Animals ; Antigens, Protozoan/immunology ; Case-Control Studies ; Dog Diseases/blood ; Dog Diseases/diagnosis ; Dog Diseases/immunology ; Dogs ; Enzyme-Linked Immunosorbent Assay/veterinary ; Epitopes, B-Lymphocyte ; Humans ; Leishmania donovani/immunology ; Leishmania infantum/immunology ; Leishmaniasis, Visceral/blood ; Leishmaniasis, Visceral/diagnosis ; Leishmaniasis, Visceral/immunology ; Leishmaniasis, Visceral/veterinary ; Predictive Value of Tests ; Repressor Proteins/immunology ; Reproducibility of Results ; Serologic Tests/veterinary
    Chemical Substances Antigens, Protozoan ; Epitopes, B-Lymphocyte ; Repressor Proteins ; prohibitin
    Language English
    Publishing date 2019-08-07
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2019.112641
    Database MEDical Literature Analysis and Retrieval System OnLINE

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