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  1. Article ; Online: Picture Exchange Communication System (PECS) Implementation Program for children with autism spectrum disorder.

    Tamanaha, Ana Carina / Olivatti, Dayane Oliveira Felicio / Silva, Simoni Camilo da / Vieira, Soraia Cunha Peixoto / Perissinoto, Jacy

    CoDAS

    2023  Volume 35, Issue 4, Page(s) e20210305

    Abstract: Purpose: The aim of this study was to evaluate a program for implementing of the PECS in children with non-verbal ASD or with minimal verbalization in a school clinic belonging to the Unified Health System - SUS.: Methods: This is a longitudinal ... ...

    Title translation Programa de Implementação do Picture Exchange Communication System (PECS) para crianças com Transtorno do Espectro do Autismo.
    Abstract Purpose: The aim of this study was to evaluate a program for implementing of the PECS in children with non-verbal ASD or with minimal verbalization in a school clinic belonging to the Unified Health System - SUS.
    Methods: This is a longitudinal study. The sample consisted of 22 children with nonverbal ASD or with minimal verbalization; 17 boys and 5 girls, aged 6 to 12 years old. The program consisted of 24 sessions of individual speech language therapy with the presence of the family member and followed the six phases originally proposed by the PECS Training Manual.
    Results: All children reached the first three phases. About 82% reached phase IV; 64% phase V and 19% phase VI. Family adherence was 96%.
    Conclusion: It was possible to test a PECS implementation program in 24 sessions and verify that children were able to achieve phases of discrimination and sentence construction, besides demonstrating gain in their lexical repertoire and reduction of non-adaptative behaviors.
    MeSH term(s) Male ; Female ; Humans ; Child ; Autism Spectrum Disorder ; Longitudinal Studies ; Family ; Speech Therapy ; Communication
    Language English
    Publishing date 2023-09-18
    Publishing country Brazil
    Document type Journal Article
    ISSN 2317-1782
    ISSN (online) 2317-1782
    DOI 10.1590/2317-1782/20232021305pt
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  2. Article ; Online: A novel and simple heat-based method eliminates the highly detrimental effect of xylene deparaffinization on acid-fast stains.

    Marinho, Pedro F / Vieira, Soraia L / Carvalho, Tânia G / Peleteiro, Maria C / Hanscheid, Thomas

    American journal of clinical pathology

    2023  Volume 160, Issue 1, Page(s) 81–88

    Abstract: Objectives: Histopathology is an important method for diagnosing extrapulmonary tuberculosis, yet tissue sections are often negative for mycobacteria after use of acid-fast stain (AFS). This study investigated the mechanism of AFS use and the ... ...

    Abstract Objectives: Histopathology is an important method for diagnosing extrapulmonary tuberculosis, yet tissue sections are often negative for mycobacteria after use of acid-fast stain (AFS). This study investigated the mechanism of AFS use and the detrimental effect of histologic processing-in particular, xylene deparaffinization-on AFS and mycobacterial detection.
    Methods: The target of the fluorescent Auramine O (AuO) AFS was investigated using triple staining with DNA- and RNA-specific dyes. The effect of xylene deparaffinization on the acid fastness of mycobacteria in cultures or tissue sections was studied using AuO fluorescence as a quantitative marker. The xylene method was compared with a novel, solvent-free projected-hot-air deparaffinization (PHAD).
    Results: Co-localization of AuO with DNA/RNA stains suggests that intracellular nucleic acids are the true target of AFS, producing highly specific patterns. Xylene reduces mycobacterial fluorescence significantly (P < .0001; moderate effect size, r = 0.33). The PHAD process yielded significantly higher fluorescence than xylene deparaffinization in tissues (P < .0001; large effect size, r = 0.85).
    Conclusions: Auramine O can be applied for nucleic acid staining of mycobacteria in tissues producing typical beaded patterns. Acid-fast staining depends heavily on the integrity of the mycobacterial cell wall, which xylene appears to damage. A solvent-free tissue deparaffinization method has the potential to increase mycobacterial detection significantly.
    MeSH term(s) Humans ; Xylenes ; Benzophenoneidum ; Hot Temperature ; Coloring Agents ; Staining and Labeling ; Mycobacterium ; RNA
    Chemical Substances Xylenes ; Benzophenoneidum (2465-27-2) ; Coloring Agents ; RNA (63231-63-0)
    Language English
    Publishing date 2023-05-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2944-0
    ISSN 1943-7722 ; 0002-9173
    ISSN (online) 1943-7722
    ISSN 0002-9173
    DOI 10.1093/ajcp/aqad016
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  3. Article: Spatial and Temporal Distribution of the Multi-element Signatures of the Estuarine Non-indigenous Bivalve Ruditapes philippinarum

    Vieira, Soraia / Barrulas, Pedro / Chainho, Paula / Dias, Cristina Barrocas / Sroczyńska, Katarzyna / Adão, Helena

    Biological trace element research. 2022 Jan., v. 200, no. 1

    2022  

    Abstract: Filter-feeder bivalves such as non-indigenous Ruditapes philippinarum absorb and accumulate metals, resulting in multi-element profiles. The goal of this study was to analyse spatial and temporal distributions of the multi-element signatures in R. ... ...

    Abstract Filter-feeder bivalves such as non-indigenous Ruditapes philippinarum absorb and accumulate metals, resulting in multi-element profiles. The goal of this study was to analyse spatial and temporal distributions of the multi-element signatures in R. philippinarum populations of the Tagus and Sado estuaries (SW coast, Portugal). The clam and sediment samples were collected at three sampling sites in each estuary, on three sampling occasions, and the analysis were done by ICP-MS (inductively coupled plasma mass spectrometry). The chemical elements were categorized according to estuarine geomorphology sources (Se, Co, Ni and Cu), elements with function in metabolic processes of the clams (Mn, Fe, Zn and Cr) and elements derived from the anthropogenic inputs (As, Pb and Cd). Zinc, Co, Ni and Pb were the main contributors for the chemical signatures of Tagus estuary populations, whilst for the Sado estuary populations were Cu, Fe, Cr, As and Cd. They were representative of all elemental categories and proved to be spatial and temporal habitat discriminators of bivalves’ estuarine populations. The multi-element signatures of R. philippinarum as a natural tag derived from the physical and chemical conditions of its habitat is a potential rapid tool to use in ecological monitoring and habitat assessment.
    Keywords Ruditapes philippinarum ; atomic absorption spectrometry ; biological assessment ; clams ; coasts ; estuaries ; habitats ; research ; sediments ; zinc ; Portugal
    Language English
    Dates of publication 2022-01
    Size p. 385-401.
    Publishing place Springer US
    Document type Article
    ZDB-ID 445336-0
    ISSN 1559-0720 ; 0163-4984
    ISSN (online) 1559-0720
    ISSN 0163-4984
    DOI 10.1007/s12011-021-02629-x
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  4. Article ; Online: Spatial and Temporal Distribution of the Multi-element Signatures of the Estuarine Non-indigenous Bivalve Ruditapes philippinarum.

    Vieira, Soraia / Barrulas, Pedro / Chainho, Paula / Dias, Cristina Barrocas / Sroczyńska, Katarzyna / Adão, Helena

    Biological trace element research

    2021  Volume 200, Issue 1, Page(s) 385–401

    Abstract: Filter-feeder bivalves such as non-indigenous Ruditapes philippinarum absorb and accumulate metals, resulting in multi-element profiles. The goal of this study was to analyse spatial and temporal distributions of the multi-element signatures in R. ... ...

    Abstract Filter-feeder bivalves such as non-indigenous Ruditapes philippinarum absorb and accumulate metals, resulting in multi-element profiles. The goal of this study was to analyse spatial and temporal distributions of the multi-element signatures in R. philippinarum populations of the Tagus and Sado estuaries (SW coast, Portugal). The clam and sediment samples were collected at three sampling sites in each estuary, on three sampling occasions, and the analysis were done by ICP-MS (inductively coupled plasma mass spectrometry). The chemical elements were categorized according to estuarine geomorphology sources (Se, Co, Ni and Cu), elements with function in metabolic processes of the clams (Mn, Fe, Zn and Cr) and elements derived from the anthropogenic inputs (As, Pb and Cd). Zinc, Co, Ni and Pb were the main contributors for the chemical signatures of Tagus estuary populations, whilst for the Sado estuary populations were Cu, Fe, Cr, As and Cd. They were representative of all elemental categories and proved to be spatial and temporal habitat discriminators of bivalves' estuarine populations. The multi-element signatures of R. philippinarum as a natural tag derived from the physical and chemical conditions of its habitat is a potential rapid tool to use in ecological monitoring and habitat assessment.
    MeSH term(s) Animals ; Bivalvia ; Environmental Monitoring ; Estuaries ; Geologic Sediments ; Metals, Heavy/analysis ; Water Pollutants, Chemical/analysis
    Chemical Substances Metals, Heavy ; Water Pollutants, Chemical
    Language English
    Publishing date 2021-02-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 445336-0
    ISSN 1559-0720 ; 0163-4984
    ISSN (online) 1559-0720
    ISSN 0163-4984
    DOI 10.1007/s12011-021-02629-x
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  5. Article ; Online: The fluorescent acid-fast stain, auramine-O, stains schistosome eggs and may be an aid for detection. An old technique with a useful future?

    Hanscheid, Thomas / Vieira, Soraia / Ferreira, Pedro / Carvalho, Tânia / Belo, Silvana / Grobusch, Martin P

    Travel medicine and infectious disease

    2020  Volume 35, Page(s) 101569

    MeSH term(s) Animals ; Benzophenoneidum/chemistry ; Coloring Agents ; Microscopy, Fluorescence/methods ; Ovum ; Schistosoma haematobium ; Schistosoma mansoni ; Schistosomiasis haematobia/diagnosis ; Schistosomiasis mansoni/diagnosis ; Staining and Labeling/methods
    Chemical Substances Coloring Agents ; Benzophenoneidum (2465-27-2)
    Language English
    Publishing date 2020-01-30
    Publishing country Netherlands
    Document type Letter
    ZDB-ID 2170891-5
    ISSN 1873-0442 ; 1477-8939
    ISSN (online) 1873-0442
    ISSN 1477-8939
    DOI 10.1016/j.tmaid.2020.101569
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  6. Article ; Online: Ziehl-Neelsen in Schistosomiasis: Much More Than Staining the Shell and Species Identification.

    Vieira, Soraia / Belo, Silvana / Hänscheid, Thomas

    The American journal of tropical medicine and hygiene

    2016  Volume 94, Issue 4, Page(s) 699–700

    MeSH term(s) Animals ; Coloring Agents/therapeutic use ; Egg Shell/ultrastructure ; Humans ; Microscopy ; Ovum ; Schistosoma mansoni/ultrastructure ; Schistosomiasis/diagnosis ; Schistosomiasis/parasitology ; Schistosomiasis mansoni/diagnosis ; Schistosomiasis mansoni/parasitology
    Chemical Substances Coloring Agents
    Language English
    Publishing date 2016-04-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2942-7
    ISSN 1476-1645 ; 0002-9637
    ISSN (online) 1476-1645
    ISSN 0002-9637
    DOI 10.4269/ajtmh.15-0798
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