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  1. Article ; Online: O

    Vigneshwari, Loganathan / Balasubramaniam, Boopathi / Sethupathy, Sivasamy / Pandian, Shunmugiah Karutha / Balamurugan, Krishnaswamy

    RSC advances

    2018  Volume 8, Issue 41, Page(s) 23089–23100

    Abstract: Glycosylation is one of the most prevalent post-translational modifications in biological systems. ... ...

    Abstract Glycosylation is one of the most prevalent post-translational modifications in biological systems. In
    Language English
    Publishing date 2018-06-26
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/c8ra00279g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: l-Ascorbyl 2,6-dipalmitate inhibits biofilm formation and virulence in methicillin-resistant Staphylococcus aureus and prevents triacylglyceride accumulation in Caenorhabditis elegans

    Sethupathy, Sivasamy / Vigneshwari, Loganathan / Valliammai, Alaguvel / Balamurugan, Krishnaswamy / Pandian, Shunmugiah Karutha

    RSC advances. 2017 May 02, v. 7, no. 38

    2017  

    Abstract: In the present study, the antibiofilm, antipathogenic and anticarotenogenic potential of l-ascorbyl 2,6-dipalmitate (ADP) against methicillin-resistant Staphylococcus aureus (MRSA) has been evaluated. ADP inhibited biofilm formation by MRSA in a ... ...

    Abstract In the present study, the antibiofilm, antipathogenic and anticarotenogenic potential of l-ascorbyl 2,6-dipalmitate (ADP) against methicillin-resistant Staphylococcus aureus (MRSA) has been evaluated. ADP inhibited biofilm formation by MRSA in a concentration-dependent manner. Light and confocal laser scanning microscopic analyses further confirmed the potent antibiofilm activity of ADP. Furthermore, ADP treatment inhibited virulence factors without any influence on the growth/metabolic activity of MRSA. ADP treatment also affected the survival of MRSA in the presence of hydrogen peroxide, methylene blue and whole blood, and modulated the expression of genes involved in biofilm formation and virulence. The combination of ADP with antibiotics efficiently protects Caenorhabditis elegans from MRSA infection. Compounds that inhibit staphyloxanthin synthesis are known to inhibit triglyceride accumulation in eukaryotes. Hence in the current study, the anti-obesity potential of ADP was also evaluated using the model nematode C. elegans. The results revealed the ability of ADP to mitigate triacylglyceride accumulation without affecting food consumption or reproduction. FTIR analysis also confirmed the reduction of fat accumulation. qPCR analysis revealed the ability of ADP to interfere with the expression of genes involved in fatty acid synthesis and insulin signalling. In addition, molecular docking analysis predicted the ability of ADP to interact with proteins involved in staphyloxanthin and oleic acid biosynthesis and stearoyl-coenzyme A desaturase-1 in MRSA, C. elegans and humans, respectively. The results obtained in the present study suggest that ADP could be utilized as a potent antipathogenic and anti-obesity agent.
    Keywords Caenorhabditis elegans ; Fourier transform infrared spectroscopy ; anti-obesity agents ; antibiotic resistance ; antimicrobial properties ; biochemical pathways ; biofilm ; biosynthesis ; blood ; computer simulation ; eukaryotic cells ; food consumption ; gene expression ; humans ; hydrogen peroxide ; insulin ; methicillin ; methicillin-resistant Staphylococcus aureus ; methylene blue ; models ; oleic acid ; proteins ; quantitative polymerase chain reaction ; reproduction ; triacylglycerols ; virulence
    Language English
    Dates of publication 2017-0502
    Size p. 23392-23406.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/c7ra02934a
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: A MAP Kinase pathway in Caenorhabditis elegans is required for defense against infection by opportunistic Proteus species.

    JebaMercy, Gnanasekaran / Vigneshwari, Loganathan / Balamurugan, Krishnaswamy

    Microbes and infection

    2013  Volume 15, Issue 8-9, Page(s) 550–568

    Abstract: Caenorhabditis elegans innate immunity requires a conserved mitogen activated protein kinase (MAPK) pathway that regulates the basal and pathogen-induced expression of immune effectors. Being in the group of opportunistic pathogens, Proteus spp. cause ... ...

    Abstract Caenorhabditis elegans innate immunity requires a conserved mitogen activated protein kinase (MAPK) pathway that regulates the basal and pathogen-induced expression of immune effectors. Being in the group of opportunistic pathogens, Proteus spp. cause large number of nosocomial infections. Since, Proteus spp. do not cause death in wild type C. elegans, to understand the role and contribution of MAP Kinase pathway, the mutants (sek-1 and pmk-1) of this pathway were employed. Physiological experiments revealed that the Proteus spp. were able to kill MAP Kinase pathway mutant's C. elegans significantly. To understand the involvement of innate immune pathways specific players at the mRNA level, the regulation of few candidate antimicrobial genes were kinetically investigated during Proteus spp. infections. Real-time PCR analysis indicated a regulation of few candidate immune regulatory genes (F08G5.6, lys-7, nlp-29, ATF-7 and daf-16) during the course of Proteus spp. infections. In addition, the lipopolysaccharides (LPS) isolated from Proteus mirabilis upon exposure to mutant C. elegans showed modifications at their functional regions suggesting that the pathogen modifies its internal machinery according to the specific host for effective pathogenesis.
    MeSH term(s) Animals ; Caenorhabditis elegans/immunology ; Caenorhabditis elegans/microbiology ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Gene Expression Profiling ; Gene Knockout Techniques ; Immunity, Innate ; MAP Kinase Kinase 4/genetics ; MAP Kinase Kinase 4/metabolism ; Mitogen-Activated Protein Kinases/genetics ; Mitogen-Activated Protein Kinases/metabolism ; Opportunistic Infections/immunology ; Proteus Infections/immunology ; Proteus mirabilis/immunology ; Signal Transduction ; Survival Analysis
    Chemical Substances Caenorhabditis elegans Proteins ; Mitogen-Activated Protein Kinases (EC 2.7.11.24) ; Pmk-1 protein, C elegans (EC 2.7.11.24) ; MAP Kinase Kinase 4 (EC 2.7.12.2) ; sek-1 protein, C elegans (EC 2.7.12.2)
    Language English
    Publishing date 2013-04-15
    Publishing country France
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1465093-9
    ISSN 1769-714X ; 1286-4579
    ISSN (online) 1769-714X
    ISSN 1286-4579
    DOI 10.1016/j.micinf.2013.03.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5014: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article: A MAP Kinase pathway in Caenorhabditis elegans is required for defense against infection by opportunistic Proteus species

    JebaMercy, Gnanasekaran / Vigneshwari, Loganathan / Balamurugan, Krishnaswamy

    Microbes and Infection. 2013 , v. 15, no. 8-9

    2013  

    Abstract: Caenorhabditis elegans innate immunity requires a conserved mitogen activated protein kinase (MAPK) pathway that regulates the basal and pathogen-induced expression of immune effectors. Being in the group of opportunistic pathogens, Proteus spp. cause ... ...

    Abstract Caenorhabditis elegans innate immunity requires a conserved mitogen activated protein kinase (MAPK) pathway that regulates the basal and pathogen-induced expression of immune effectors. Being in the group of opportunistic pathogens, Proteus spp. cause large number of nosocomial infections. Since, Proteus spp. do not cause death in wild type C. elegans, to understand the role and contribution of MAP Kinase pathway, the mutants (sek-1 and pmk-1) of this pathway were employed. Physiological experiments revealed that the Proteus spp. were able to kill MAP Kinase pathway mutant's C. elegans significantly. To understand the involvement of innate immune pathways specific players at the mRNA level, the regulation of few candidate antimicrobial genes were kinetically investigated during Proteus spp. infections. Real-time PCR analysis indicated a regulation of few candidate immune regulatory genes (F08G5.6, lys-7, nlp-29, ATF-7 and daf-16) during the course of Proteus spp. infections. In addition, the lipopolysaccharides (LPS) isolated from Proteus mirabilis upon exposure to mutant C. elegans showed modifications at their functional regions suggesting that the pathogen modifies its internal machinery according to the specific host for effective pathogenesis.
    Keywords Caenorhabditis elegans ; Proteus mirabilis ; death ; gene expression ; innate immunity ; lipopolysaccharides ; messenger RNA ; mitogen-activated protein kinase ; mutants ; pathogenesis ; pathogens ; quantitative polymerase chain reaction ; regulator genes
    Language English
    Dates of publication 2013-07
    Size p. 550-568.
    Publishing place Elsevier Masson SAS
    Document type Article
    ZDB-ID 1465093-9
    ISSN 1769-714X ; 1286-4579
    ISSN (online) 1769-714X
    ISSN 1286-4579
    DOI 10.1016/j.micinf.2013.03.009
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 99/701: Show issues

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  5. Article ; Online: Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins.

    Sethupathy, Sivasamy / Ananthi, Sivagnanam / Selvaraj, Anthonymuthu / Shanmuganathan, Balakrishnan / Vigneshwari, Loganathan / Balamurugan, Krishnaswamy / Mahalingam, Sundarasamy / Pandian, Shunmugiah Karutha

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 16328

    Abstract: Serratia marcescens is one of the important nosocomial pathogens which rely on quorum sensing (QS) to regulate the production of biofilm and several virulence factors. Hence, blocking of QS has become a promising approach to quench the virulence of S. ... ...

    Abstract Serratia marcescens is one of the important nosocomial pathogens which rely on quorum sensing (QS) to regulate the production of biofilm and several virulence factors. Hence, blocking of QS has become a promising approach to quench the virulence of S. marcescens. For the first time, QS inhibitory (QSI) and antibiofilm potential of Actinidia deliciosa have been explored against S. marcescens clinical isolate (CI). A. deliciosa pulp extract significantly inhibited the virulence and biofilm production without any deleterious effect on the growth. Vanillic acid was identified as an active lead responsible for the QSI activity. Addition of vanillic acid to the growth medium significantly affected the QS regulated production of biofilm and virulence factors in a concentration dependent mode in S. marcescens CI, ATCC 14756 and MG1. Furthermore vanillic acid increased the survival of Caenorhabditis elegans upon S. marcescens infection. Proteomic analysis and mass spectrometric identification of differentially expressed proteins revealed the ability of vanillic acid to modulate the expression of proteins involved in S-layers, histidine, flagellin and fatty acid production. QSI potential of the vanillic acid observed in the current study paves the way for exploring it as a potential therapeutic candidate to treat S. marcescens infections.
    MeSH term(s) Actinidia/chemistry ; Animals ; Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/metabolism ; Biofilms/drug effects ; Caenorhabditis elegans/microbiology ; Chromatography, Liquid ; Dose-Response Relationship, Drug ; Fatty Acids/biosynthesis ; Flagellin/metabolism ; Mass Spectrometry ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Proteome ; Proteomics/methods ; Quorum Sensing/drug effects ; Serratia marcescens/drug effects ; Serratia marcescens/pathogenicity ; Serratia marcescens/physiology ; Vanillic Acid/chemistry ; Vanillic Acid/pharmacology ; Virulence/drug effects ; Virulence Factors
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; Fatty Acids ; Plant Extracts ; Proteome ; Virulence Factors ; Flagellin (12777-81-0) ; Vanillic Acid (GM8Q3JM2Y8)
    Language English
    Publishing date 2017-11-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-16507-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Biogenic synthesis of silver nanoparticles using Piper betle aqueous extract and evaluation of its anti-quorum sensing and antibiofilm potential against uropathogens with cytotoxic effects: an in vitro and in vivo approach.

    Srinivasan, Ramanathan / Vigneshwari, Loganathan / Rajavel, Tamilselvam / Durgadevi, Ravindran / Kannappan, Arunachalam / Balamurugan, Krishnaswamy / Pandima Devi, Kasi / Veera Ravi, Arumugam

    Environmental science and pollution research international

    2017  Volume 25, Issue 11, Page(s) 10538–10554

    Abstract: Urinary tract infections are the utmost common bacterial infections caused by Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens. These uropathogens resist the action of several antibiotics due to their ability to form ... ...

    Abstract Urinary tract infections are the utmost common bacterial infections caused by Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Serratia marcescens. These uropathogens resist the action of several antibiotics due to their ability to form biofilms. Most of these bacterial pathogens use the quorum sensing (QS) machinery to co-ordinate their cells and regulate several virulence factors and biofilm formation. On the other hand, the anti-quorum sensing (anti-QS) and antibiofilm potential of silver nanoparticles have been well reported against certain bacterial pathogens, but to the best of our knowledge, no report is available against the pathogenicity of uropathogens in particular S. marcescens and P. mirabilis. Therefore, the present study is primarily focused on the anti-QS and antibiofilm potential of Piper betle-based synthesized silver nanoparticles (PbAgNPs) against S. marcescens and P. mirabilis. Initially, the silver nanoparticles were synthesized by the aqueous extract of P. betle and characterized by UV-absorbance spectroscopy, XRD, FT-IR, SEM, TEM, and DLS. The synthesized silver nanoparticles were assessed for their anti-QS activity and the obtained results revealed that the PbAgNPs inhibited the QS-mediated virulence factors such as prodigiosin, protease, biofilm formation, exopolysaccharides and hydrophobicity productions in uropathogens. The gene expression analysis divulged the downregulation of fimA, fimC, flhD, and bsmB genes in S. marcescens and flhB, flhD, and rsbA genes in P. mirabilis, respectively. The in vivo Caenorhabditis elegans assays revealed the non-toxic and anti-adherence efficiency of PbAgNPs. Furthermore, the non-toxic effect of PbAgNPs was also confirmed through peripheral blood mononuclear cells and normal lung epithelial cells. Therefore, the contemporary study demonstrates the use of PbAgNPs as a possible alternative toward conventional antibiotics in controlling QS and biofilm-related uropathogen infections.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Biofilms/drug effects ; Escherichia coli/drug effects ; Leukocytes, Mononuclear/drug effects ; Metal Nanoparticles/chemistry ; Piper betle ; Prodigiosin/chemistry ; Proteus mirabilis/drug effects ; Pseudomonas aeruginosa/drug effects ; Quorum Sensing/drug effects ; Serratia marcescens/drug effects ; Silver/chemistry ; Spectroscopy, Fourier Transform Infrared ; Urinary Tract Infections/microbiology ; Virulence/drug effects ; Virulence Factors/chemistry
    Chemical Substances Anti-Bacterial Agents ; Virulence Factors ; Silver (3M4G523W1G) ; Prodigiosin (OL369FU7CJ)
    Language English
    Publishing date 2017-12-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-017-1049-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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