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  1. Article ; Online: Depletion of skeletal muscle satellite cells attenuates pathology in muscular dystrophy

    Justin G. Boyer / Jiuzhou Huo / Sarah Han / Julian R. Havens / Vikram Prasad / Brian L. Lin / David A. Kass / Taejeong Song / Sakthivel Sadayappan / Ramzi J. Khairallah / Christopher W. Ward / Jeffery D. Molkentin

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 10

    Abstract: Boyer et al. created genetic mouse models of muscular dystrophy in which satellite cells were selectively depleted. The depletion of satellite cells at select times was protective. Myofibers no longer had plasma membrane instability leading to tissue ... ...

    Abstract Boyer et al. created genetic mouse models of muscular dystrophy in which satellite cells were selectively depleted. The depletion of satellite cells at select times was protective. Myofibers no longer had plasma membrane instability leading to tissue wasting in the muscular dystrophies.
    Keywords Science ; Q
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Knowledge, Attitude and Practice with Regard to Gram stain, as a Preliminary Test for Clinical Diagnosis among the Internees and Postgraduates, in a Tertiary Care Teaching Hospital

    Raja Vikram Prasad / Mohammad Abdul Mushtaq Pasha / Afsar Fatima / Deepa Latha Ciddhavaduta / Veeresappa Doddi Kummari

    Journal of Evidence Based Medicine and Healthcare, Vol 7, Iss 50, Pp 2987-

    2020  Volume 2991

    Abstract: BACKGROUND Hans Christian Gram, a Danish pathologist originally devised the Gram staining method in 1882 for identification of Gram positive and Gram-negative bacteria and is the gold standard method for preliminary detection before inoculating for ... ...

    Abstract BACKGROUND Hans Christian Gram, a Danish pathologist originally devised the Gram staining method in 1882 for identification of Gram positive and Gram-negative bacteria and is the gold standard method for preliminary detection before inoculating for culture and sensitivity for better treatment outcome. In the present era, use of irrational antimicrobials has led to antimicrobial resistance which can be resolved by routine usage of Gram stain. METHODS This is a cross sectional study of 2 months duration (May 2020 - June 2020) with 172 subjects. Institutional ethical clearance and pharmaco-vigilance program of India permission was obtained. All interns and post-graduates who were available at the time of interview, and who gave written informed consent were included in the study. Those who did not give written informed consent and those who were absent or unavailable were excluded. Pre tested questionnaire was validated using Cronbach’s alpha for internal consistency and was filled voluntarily. Data was collected, compiled and analysed for results. RESULTS Among 172 subjects who participated in the study, 99 (57 %) are females and 73 (43 %) are males. 88 (51 %) are post-graduates and 84 (49 %) are interns. 100 % had knowledge about Gram stain, 47 (27 %) participants knew about procedure timing as 5 minutes for Gram staining and 26 (15 %) participants thought that test can be done at bedside. 90 (52 %) knew that test can be done for all specimens. 169 (98 %) had knowledge on the Gram stain which could help for provisional diagnosis. 119 (69 %) agreed that test was very cost effective. 28 (16 %) agreed that Gram stain was a better test for provisional diagnosis. 63 (36.6 %) were practicing regularly and 10 (6 %) were practicing Gram stain for appropriate antimicrobial use. CONCLUSIONS The present study concluded 100 % of the study participants had knowledge with regard to Gram staining, 27 % knew that the time taken was 5 minutes to perform test. 15 % knew that it can be done at bedside. 63 (36.6 %) were practicing regularly and 10 (6 %) were practicing as initial step for all clinical confirmations for appropriate antimicrobial treatment for better outcome.
    Keywords gram stain ; preliminary test ; antimicrobial stewardship ; evidence based medicine ; General works ; R5-130.5
    Subject code 150
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Level Up Business Center
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains

    Alyssa A. W. Cramer / Vikram Prasad / Einar Eftestøl / Taejeong Song / Kenth-Arne Hansson / Hannah F. Dugdale / Sakthivel Sadayappan / Julien Ochala / Kristian Gundersen / Douglas P. Millay

    Nature Communications, Vol 11, Iss 1, Pp 1-

    2020  Volume 14

    Abstract: Skeletal muscle is composed of syncytial myofibres, each containing hundreds of nuclei. Through genetic reduction of the number of nuclei per myofibre, the authors confirm that more nuclei produce larger cells but myofibres with fewer nuclei adaptively ... ...

    Abstract Skeletal muscle is composed of syncytial myofibres, each containing hundreds of nuclei. Through genetic reduction of the number of nuclei per myofibre, the authors confirm that more nuclei produce larger cells but myofibres with fewer nuclei adaptively compensate leading to larger and functional myonuclear domains.
    Keywords Science ; Q
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development

    Malgorzata E. Quinn / Qingnian Goh / Mitsutoshi Kurosaka / Dilani G. Gamage / Michael J. Petrany / Vikram Prasad / Douglas P. Millay

    Nature Communications, Vol 8, Iss 1, Pp 1-

    2017  Volume 9

    Abstract: Cellular fusion is fundamental for skeletal muscle development. Here the authors show that myomerger is expressed in myoblasts, is essential for myoblast fusion in mice, and in co-operation with myomaker confers fusogenic ability to non-fusogenic cells. ...

    Abstract Cellular fusion is fundamental for skeletal muscle development. Here the authors show that myomerger is expressed in myoblasts, is essential for myoblast fusion in mice, and in co-operation with myomaker confers fusogenic ability to non-fusogenic cells.
    Keywords Science ; Q
    Language English
    Publishing date 2017-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Mitsugumin 29 regulates t-tubule architecture in the failing heart

    Robert N. Correll / Jeffrey M. Lynch / Tobias G. Schips / Vikram Prasad / Allen J. York / Michelle A. Sargent / Didier X. P. Brochet / Jianjie Ma / Jeffery D. Molkentin

    Scientific Reports, Vol 7, Iss 1, Pp 1-

    2017  Volume 10

    Abstract: Abstract Transverse tubules (t-tubules) are uniquely-adapted membrane invaginations in cardiac myocytes that facilitate the synchronous release of Ca2+ from internal stores and subsequent myofilament contraction, although these structures become ... ...

    Abstract Abstract Transverse tubules (t-tubules) are uniquely-adapted membrane invaginations in cardiac myocytes that facilitate the synchronous release of Ca2+ from internal stores and subsequent myofilament contraction, although these structures become disorganized and rarefied in heart failure. We previously observed that mitsugumin 29 (Mg29), an important t-tubule organizing protein in skeletal muscle, was induced in the mouse heart for the first time during dilated cardiomyopathy with heart failure. Here we generated cardiac-specific transgenic mice expressing Mg29 to model this observed induction in the failing heart. Interestingly, expression of Mg29 in the hearts of Csrp3 null mice (encoding muscle LIM protein, MLP) partially restored t-tubule structure and preserved cardiac function as measured by invasive hemodynamics, without altering Ca2+ spark frequency. Conversely, gene-deleted mice lacking both Mg29 and MLP protein showed a further reduction in t-tubule organization and accelerated heart failure. Thus, induction of Mg29 in the failing heart is a compensatory response that directly counteracts the well-characterized loss of t-tubule complexity and reduced expression of anchoring proteins such as junctophilin-2 (Jph2) that normally occur in this disease. Moreover, preservation of t-tubule structure by Mg29 induction significantly increases the function of the failing heart.
    Keywords Medicine ; R ; Science ; Q
    Subject code 572
    Language English
    Publishing date 2017-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: SERCA2 Haploinsufficiency in a Mouse Model of Darier Disease Causes a Selective Predisposition to Heart Failure

    Vikram Prasad / John N. Lorenz / Valerie M. Lasko / Michelle L. Nieman / Wei Huang / Yigang Wang / David W. Wieczorek / Gary E. Shull

    BioMed Research International, Vol

    2015  Volume 2015

    Abstract: Null mutations in one copy of ATP2A2, the gene encoding sarco/endoplasmic reticulum Ca2+-ATPase isoform 2 (SERCA2), cause Darier disease in humans, a skin condition involving keratinocytes. Cardiac function appears to be unimpaired in Darier disease ... ...

    Abstract Null mutations in one copy of ATP2A2, the gene encoding sarco/endoplasmic reticulum Ca2+-ATPase isoform 2 (SERCA2), cause Darier disease in humans, a skin condition involving keratinocytes. Cardiac function appears to be unimpaired in Darier disease patients, with no evidence that SERCA2 haploinsufficiency itself causes heart disease. However, SERCA2 deficiency is widely considered a contributing factor in heart failure. We therefore analyzed Atp2a2 heterozygous mice to determine whether SERCA2 haploinsufficiency can exacerbate specific heart disease conditions. Despite reduced SERCA2a levels in heart, Atp2a2 heterozygous mice resembled humans in exhibiting normal cardiac physiology. When subjected to hypothyroidism or crossed with a transgenic model of reduced myofibrillar Ca2+-sensitivity, SERCA2 deficiency caused no enhancement of the disease state. However, when combined with a transgenic model of increased myofibrillar Ca2+-sensitivity, SERCA2 haploinsufficiency caused rapid onset of hypertrophy, decompensation, and death. These effects were associated with reduced expression of the antiapoptotic Hax1, increased levels of the proapoptotic genes Chop and Casp12, and evidence of perturbations in energy metabolism. These data reveal myofibrillar Ca2+-sensitivity to be an important determinant of the cardiac effects of SERCA2 haploinsufficiency and raise the possibility that Darier disease patients are more susceptible to heart failure under certain conditions.
    Keywords Medicine ; R
    Subject code 610
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article: Efficient Intracellular Delivery of Native Proteins

    D’Astolfo, Diego S / Romina J. Pagliero / Anita Pras / Wouter R. Karthaus / Hans Clevers / Vikram Prasad / Robert Jan Lebbink / Holger Rehmann / Niels Geijsen

    Cell. 2015 Apr. 23, v. 161

    2015  

    Abstract: Modulation of protein function is used to intervene in cellular processes but is often done indirectly by means of introducing DNA or mRNA encoding the effector protein. Thus far, direct intracellular delivery of proteins has remained challenging. We ... ...

    Abstract Modulation of protein function is used to intervene in cellular processes but is often done indirectly by means of introducing DNA or mRNA encoding the effector protein. Thus far, direct intracellular delivery of proteins has remained challenging. We developed a method termed iTOP, for induced transduction by osmocytosis and propanebetaine, in which a combination of NaCl hypertonicity-induced macropinocytosis and a transduction compound (propanebetaine) induces the highly efficient transduction of proteins into a wide variety of primary cells. We demonstrate that iTOP is a useful tool in systems in which transient cell manipulation drives permanent cellular changes. As an example, we demonstrate that iTOP can mediate the delivery of recombinant Cas9 protein and short guide RNA, driving efficient gene targeting in a non-integrative manner.
    Keywords DNA ; gene targeting ; messenger RNA ; proteins ; sodium chloride
    Language English
    Dates of publication 2015-0423
    Size p. 674-690.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2015.03.028
    Database NAL-Catalogue (AGRICOLA)

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