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Article ; Online: Virulence-Associated Genes and Antimicrobial Resistance of Aeromonas hydrophila Isolates from Animal, Food, and Human Sources in Brazil

Emily Moraes Roges / Verônica Dias Gonçalves / Maira Duarte Cardoso / Marcia Lima Festivo / Salvatore Siciliano / Lucia Helena Berto / Virginia Leo de Almeida Pereira / Dalia dos Prazeres Rodrigues / Maria Helena Cosendey de Aquino

BioMed Research International, Vol

2020 Volume 2020

Abstract: Aeromonads are natural inhabitants of aquatic environments and may be associated with various human or animal diseases. Its pathogenicity is complex and multifactorial and is associated with many virulence factors. In this study, 110 selected Aeromonas ... ...

Abstract	Aeromonads are natural inhabitants of aquatic environments and may be associated with various human or animal diseases. Its pathogenicity is complex and multifactorial and is associated with many virulence factors. In this study, 110 selected Aeromonas hydrophila isolates isolated from food, animals, and human clinical material from 2010 to 2015 were analyzed. Antimicrobial susceptibility testing was performed by the disk diffusion method, and polymerase chain reaction was conducted to investigate the virulence genes hemolysin (hlyA), cytotoxic enterotoxin (act), heat-labile cytotonic enterotoxin (alt), aerolysin (aerA), and DNase-nuclease (exu). At least 92.7% of the isolates had one of the investigated virulence genes. Twenty different virulence profiles among the isolates were recognized, and the five investigated virulence genes were observed in four isolates. Human source isolates showed greater diversity than food and animal sources. Antimicrobial resistance was observed in 46.4% of the isolates, and multidrug resistance was detected in 3.6% of the isolates. Among the 120 isolates, 45% were resistant to cefoxitin; 23.5% to nalidixic acid; 16.6% to tetracycline; 13.7% to cefotaxime and imipenem; 11.8% to ceftazidime; 5.9% to amikacin, gentamicin, and sulfamethoxazole-trimethoprim; and 3.9% to ciprofloxacin and nitrofurantoin. Overall, the findings of our study indicated the presence of virulence genes and that antimicrobial resistance in A. hydrophila isolates in this study is compatible with potentially pathogenic bacteria. This information will allow us to recognize the potential risk through circulating isolates in animal health and public health and the spread through the food chain offering subsidies for appropriate sanitary actions.
Keywords	Medicine ; R
Subject code	630
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Performance, serologic and tracheal responses of laying hens exposed to F strain of Mycoplasma gallisepticum

Leandro dos Santos Machado / Dayse Lima da Costa Abreu / Mosar Lemos / Rogério Tortelly / Jorge Couto Pimentel / Luis Sesti / Virginia Léo de Almeida Pereira / Elmiro Rosendo do Nascimento

Arquivos do Instituto Biológico, Vol 84, Iss

2018

Abstract: ABSTRACT: The F strain of Mycoplasma gallisepticum (MG-F) protects chickens against mycoplasma infections, in which monitoring is made by serology and histopathology of trachea. This trial used 90 chickens, being 30 unvaccinated (G1 group), 30 eye-drop ... ...

Abstract	ABSTRACT: The F strain of Mycoplasma gallisepticum (MG-F) protects chickens against mycoplasma infections, in which monitoring is made by serology and histopathology of trachea. This trial used 90 chickens, being 30 unvaccinated (G1 group), 30 eye-drop vaccinated at 8 weeks of age with MG-F (Ceva Animal Health, São Paulo, SP, Brazil) (G2), and 30 immunized at 8 and 11 weeks of age (G3). Samples were obtained from chickens on the 8, 12, 15, 18, 20 and 24th weeks of age for the enzyme-linked immunosorbent assay (ELISA) test. Tracheal fragments were collected after necropsies on the 15 and 24th weeks of age. Up to 12 weeks, the ELISA reactions in optical density (OD) were 0.165 (G1), 0.151 (G2) and 0.151(G3), all below 0.20 and with no significant difference among groups (p > 0.05). After the 15th week, the ELISA reactions rose, yielding the following group averages by collecting dates: G1 (0.18, 0.19, 0.18, and 0.16), G2 (0.36, 0.49, 0.47, and 0.44) and G3 (0.41, 0.52, 0.59, 0.60), being the means in G2 and G3 not significantly different between than, but significantly different from G1. The initial weight (592.71, 621.33, and 594.40), the final weight (1,932.58, 1,987.59, and 1,875.20) and the weekly weight gain (11.65, 11.90, and 11.14) were not significantly different among groups. At necropsy the gross tracheal score means by group and dates were: 15th week (0.25, 0.61, and 0.54) and 24th week (0.54, 0.58, and 0.67), being these difference not significantly (p > 0.05). On microscopy, the tracheal score averages by groups G1, G2 and G3, respectively, were: 15th week (0.25, 0.32, and 0.47) and 24th week (0.07, 0.75, and 0.08). G2 yielded higher score average than G1 and G3 on the 24th week. Higher tracheal changes for G2 and G3 as compared to G1 could be ascribed to MG-F infection. There were no evident prejudicial effects on live weight, weight gain and tissue changes by applying one or two vaccination doses.
Keywords	Mycoplasma gallisepticum ; layers ; serology ; body weight ; histophatology ; Agriculture (General) ; S1-972
Subject code	630
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Instituto Biológico
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Use of RT-PCR and elisa techniques for the diagnostic of infectious bronchitis virus in broilers at slaughter

Davi de Oliveira Almeida / Rogerio Tortely / Elmiro Rosendo do Nascimento / Virginia Léo de Almeida Pereira / Sankhiros Babapoor / Mazhar Khan

Brazilian Journal of Veterinary Medicine, Vol 37, Iss 1, Pp 55-

2015 Volume 59

Abstract: ABSTRACT. Almeida D.O., Tortely R., Nascimento E.R., Khan M., Pereira V.L.A & Babapoor S. [Use of RT-PCR and elisa techniques for the diagnostic of infectious bronchitis virus in broilers at slaughter.] Uso das técnicas de RT-PCR e elisa no diagnóstico ... ...

Abstract	ABSTRACT. Almeida D.O., Tortely R., Nascimento E.R., Khan M., Pereira V.L.A & Babapoor S. [Use of RT-PCR and elisa techniques for the diagnostic of infectious bronchitis virus in broilers at slaughter.] Uso das técnicas de RT-PCR e elisa no diagnóstico da bronquite infecciosa em frangos de corte ao abate. Revista Brasileira de Medicina Veterinária, 37(1):55-59, 2015. Departamento de Saúde Coletiva Veterinária e Saúde Pública, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Santa Rosa, Niterói, RJ 24230-340, Brasil. E-mail: elmiro@vm.uff.br Avian infectious bronchitis (IB) is a viral, acute and highly contagious disease caused by infectious bronchitis virus (IBV). The disease affects broilers improvement and performance causing major economic losses in the world poultry industry. Generally, IBV infections can be diagnosed by detection of IBV itself or the specific antibody response. This study aimed to detect IBV in broilers under Sanitary Inspection by RT-PCR and ELISA, comparing the results and relating them with the average weight of the flock. Samples were collected from 40 vaccinated broiler flocks under Sanitary Inspection at slaughter. Ten birds from every flock were randomly selected and blood samples were collected for ELISA as well three birds had their trachea and caecal tonsils collected for RT- -PCR test. From 40 flocks, 30 were IBV positive by ELISA and 26 by RT-PCR, in which 15 were detected simultaneously in trachea and caecal tonsil and 5 in each sample. There was no agreement between ELISA and RT-PCR results regarding IBV diagnosis as well positivity in both tests was not statistically significant with the average weight of the flock. There was an improvement on IBV diagnosis when caecal tonsils and tracheas were used instead of just one of them. Considering the only vaccine serotype allowed by Brazilian government is the Mass serotype and its persistence in broilers would only be detected up to 28 days, possibly the studied broilers were challenge with a field strain.
Keywords	Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2015-03-01T00:00:00Z
Publisher	Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
Document type	Article ; Online
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Article ; Online: Mycoplasma spp. in captive snakes (Boa constrictor and Bothrops atrox) from Brazil

Barbara Souza Neil Magalhães / Leandro dos Santos Machado / Arthur de Almeida Figueira / Thomas Salles Dias / Thiago de Almeida Feijó / Maria Lucia Barreto / Giulia de Almeida Tuffanelli / Nathalie Costa da Cunha / Elmiro Rosendo do Nascimento / Virginia Léo de Almeida Pereira / Nádia Regina Pereira Almosny

Ciência Rural, Vol 51, Iss

2020 Volume 1

Abstract: ABSTRACT: Although rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, in the order Squamata, three reports of these microorganisms causing diseases in pythons have already been reported. This study aimed to evaluate ... ...

Abstract	ABSTRACT: Although rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, in the order Squamata, three reports of these microorganisms causing diseases in pythons have already been reported. This study aimed to evaluate the occurrence of Mycoplasma species in captive snakes. A total of 26 snakes of the families Pythonidae (13), Boidae (7), Viperidae (5) and Colubridae (1) from RioZoo, Brazil, were evaluated. Animals were examined to determine clinical signs consistent with any infectious disease. Tracheal swab samples from snakes were collected in Frey medium and analyzed for the presence of Mycoplasma spp.by isolation and a genus-specific PCR. DNA sequencing analyses of six positive samples by PCR were carried out to identify the species. Using isolation 19.23% (5/26) was positive, while 65.38% (17/26) of the animals were positive by PCR. Based on the analyses of the six sequences obtained, there was similarity with a Mycoplasma spp. previously described in a phyton and, M. agassizii and M. testudineum reported in chelonians. This is the first report of Mycoplasma spp. in animals of the families Boidae and Viperidae. Mycoplasma spp. were detected in snakes with and without clinical signs. The mycoplasmas reported resented identity (range, 95% to 100%) to others already described in reptiles. There was no relationship between the presence of Mycoplasma spp. and clinical signs.
Keywords	Mycoplasmosis ; Mollicutes ; Order Squamata ; Boidae ; Viperidae ; Agriculture ; S ; Agriculture (General) ; S1-972
Subject code	630
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Universidade Federal de Santa Maria
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Prevalence and fluoroquinolones resistance of Campylobacter and Salmonella isolates from poultry carcasses in Rio de Janeiro, Brazil

Panzenhagen, Pedro Henrique Nunes / Beatriz da Silva Frasão / Dalia dos Prazeres Rodrigues / Dayse Lima da Costa Abreu / Elmiro Rosendo do Nascimento / Maria Helena Cosendey de Aquino / Virginia Léo de Almeida Pereira / Waldemir Silva Aguiar

Food control. 2016 Mar., v. 61

2016

Abstract: To investigate the prevalence of Campylobacter and Salmonella in poultry carcasses in state of Rio de Janeiro, Brazil, 60 samples from 6 slaughterhouses were collected over a period of 6 months. A total of 82 Campylobacter isolates were obtained from ... ...

Abstract	To investigate the prevalence of Campylobacter and Salmonella in poultry carcasses in state of Rio de Janeiro, Brazil, 60 samples from 6 slaughterhouses were collected over a period of 6 months. A total of 82 Campylobacter isolates were obtained from twenty seven (45%) positive chicken carcasses, including 44 isolates (53.66%) of Campylobacter jejuni and 38 (46.34%) of Campylobacter coli. The identification of all strains was confirmed by PCR. Salmonella was isolated from 4 (6.67%) carcasses by conventional method and was detected in 5 (8.33%) of 60 chicken carcasses by PCR. Two Salmonella Albany and two Salmonella Typhimurium were identified. Antimicrobial susceptibility testing was primarily done by the disk diffusion method and later by assessing minimum inhibitory concentrations (MICs) against all the isolates. All the Campylobacter isolates were resistant to ciprofloxacin and enrofloxacin. It was observed high MIC values for enrofloxacin (64 μg/mL) in one C. jejuni and two C. coli strains, and for ciprofloxacin (≥128 μg/mL) in one C. jejuni and three C. coli strains. No Salmonella isolate was resistant to these antibiotics by both methods. These findings reveal a broad extent of fluoroquinolone resistance in Campylobacter isolates from chicken carcasses in Brazil and underline the need for prudent use of these antibiotics in poultry production to minimize the spread of fluoroquinolone resistant Campylobacter.
Keywords	antibiotic resistance ; antibiotics ; Campylobacter coli ; Campylobacter jejuni ; chicken carcasses ; ciprofloxacin ; enrofloxacin ; minimum inhibitory concentration ; polymerase chain reaction ; poultry production ; Salmonella Albany ; Salmonella Typhimurium ; slaughterhouses ; Brazil
Language	English
Dates of publication	2016-03
Size	p. 243-247.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1027805-9
ISSN	0956-7135
ISSN	0956-7135
DOI	10.1016/j.foodcont.2015.10.002
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Pathogenic potential and septic character of the Escherichia coli by identification of virulence factors iss and felA from cellulitis and offal of broiler by Sanitary Inspection

Thaís Badini Vieira / Virginia Leo de Almeida Pereira / Robson Maia Franco / Elmiro Rosendo do Nascimento / Rita de Cássia Figueira Silva / Rogério Tortelly

Brazilian Journal of Veterinary Medicine, Vol 36, Iss 2, Pp 144-

2014 Volume 152

Abstract: ABSTRACT. Vieira T.B., Pereira V.L.A., Franco R.M., do Nascimento E.R., Silva R. de C.F. & Tortelly R. [Pathogenic potential and septic character of the Escherichia coli by identification of virulence factors iss and felA from cellulitis and offal of ... ...

Abstract	ABSTRACT. Vieira T.B., Pereira V.L.A., Franco R.M., do Nascimento E.R., Silva R. de C.F. & Tortelly R. [Pathogenic potential and septic character of the Escherichia coli by identification of virulence factors iss and felA from cellulitis and offal of broiler by Sanitary Inspection.] Potencial patogênico e caráter séptico de Escherichia coli pela identificação dos fatores de virulência iss e felA em celulite e miúdos de frangos sob Inspeção Sanitária. Revista Brasileira de Medicina Veterinária, 36(2):144-152, 2014. Departamento de Medicina Veterinária. Universidade Federal do Mato Grosso, Campus Universitário de Sinop, Avenida Alexandre Ferronato, 1200, Setor Industrial, Sinop, MT 78557367, Brasil. E-mail: thais.badini@hotmail.com Brazil is the third largest chicken producer and first in exports of broiler meat. Skin lesions such as avian cellulitis are becoming increasingly frequent in large-scale production due to premises type and management of broilers, leading to total or partial condemnation at slaughter throughout the world. In this study, we used 51 broilers with typical lesions of cellulitis, diagnosed by Sanitary Inspection. Recovery of Escherichia coli strains from cellulitis lesions and offal (liver and heart) from studied broilers established the of septic characteristic of the isolates. These E. coli isolates were tested by Polymerase Chain Reaction (PCR) for the genes of the virulence factors responsible for adhesion (F11 fimbria-felA) and serum resistance (iss). The cellulitis at inspection was characterized as skin ulcer, being eight with skin thickening, color changes tending to reddish-yellow, and irregular skin surface. At cutting, gelatinous fluid and yellowish patches were seen, in some cases, with involvement of the adjacent musculature. E. coli was isolated from 50 broilers, being 19, from cellulitis, 11 from cellulitis and liver, five from cellulitis and heart, 14, from cellulitis, liver and heart, and one only from liver and heart. E. coli isolates were recovered from 96% of ...
Keywords	Veterinary medicine ; SF600-1100
Subject code	571
Language	English
Publishing date	2014-06-01T00:00:00Z
Publisher	Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
Document type	Article ; Online
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Article ; Online: Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection

Kênia de Fátima Carrijo / Elmiro Rosendo do Nascimento / Virginia Léo de Almeida Pereira / Nelson Morés / Catia Silene Klein / Leonardo Muliterno Domingues / Rogerio Tortelly

Brazilian Journal of Veterinary Medicine, Vol 36, Iss 2, Pp 188-

comparison with other diagnostic methods

2014 Volume 194

Abstract: ABSTRACT. Carrijo K.F., Nascimento E.R., Pereira V.L.A., Morés N., Klein, C.S., Domingues L.M. & Tortelly R. [Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods.] Diagnóstico da pneumonia ... ...

Abstract	ABSTRACT. Carrijo K.F., Nascimento E.R., Pereira V.L.A., Morés N., Klein, C.S., Domingues L.M. & Tortelly R. [Diagnosis of porcine enzootic pneumonia by post mortem sanitary inspection: comparison with other diagnostic methods.] Diagnóstico da pneumonia enzoótica suína pela inspeção sanitária post mortem: comparação com outros métodos de diagnóstico. Revista Brasileira de Veterinária Brasileira 36(2):188-194, 2014. Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, Av. Pará, 1720, Bloco 2T, Jardim Umuarama, Uberlândia, MG 38400-902, Brasil. E-mail: keniacarrijo@ famev.ufu.br To compare the concordance of the diagnosis of porcine enzootic pneumonia (PEP) by post-mortem Sanitary Inspection with other methods (histophatology and immunohistochemistry - IHC), were used lung tissue samples from 100 pigs slaughtered under sanitary inspection, and 50 of these had macroscopic lesions suggestive of PEP and 50 had no such lesions. These were fixed in 10% buffered formalin and processed by routine procedures for paraffin embedding and IHC technique for Mycoplasma hyopneumoniae using a monoespecific polyclonal antibody. The study demonstrating that there is concordance between the diagnosis of Sanitary Inspection with histophatology, between the diagnosis of Sanitary Inspection with IHC and histophatology with IHC. It can be conclude that when the lung has gross lesions of PEP, the probability the result is positive to M. hyopneumoniae by IHC and the presence of microscopic lesions increases. Thus, the microscopic diagnosis for PEP is feasible because it is associated to the other, so that the diagnosis given by the officials of Sanitary Inspection in slaughterhouses is not wrong; the macroscopic diagnosis is therefore a valid method for the diagnosis of PEP, it being understood this is not to say that the detection of M. hyopneumoniae.
Keywords	Veterinary medicine ; SF600-1100
Subject code	610
Language	English
Publishing date	2014-06-01T00:00:00Z
Publisher	Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
Document type	Article ; Online
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Article ; Online: Epidemiological situation of avian mycoplamosis in the State of Rio de Janeiro, Brazil

Valéria Christina Magalhães Teixeira / Daniela de Queiroz Baptista / Fernanda Carla Carlos / Willker Rocha de Menezes / Daniela Sabroza José / Maria Lúcia Barreto / Dayse Lima da Costa Abreu / Virginia Léo de Almeida Pereira / Elmiro Rosendo do Nascimento

Brazilian Journal of Veterinary Medicine, Vol 37, Iss 4, Pp 379-

2015 Volume 385

Abstract: ABSTRACT. Teixeira V.C.M., Baptista D.Q., Carlos F.C., Menezes W.R., José D.S., Barreto M.L., Abreu D.L.C., Pereira V.L.A. & Nascimento E.R. [Epidemiological situation of avian mycoplamosis in the State of Rio de Janeiro, Brazil.] Situação epidemiológica ...

Abstract	ABSTRACT. Teixeira V.C.M., Baptista D.Q., Carlos F.C., Menezes W.R., José D.S., Barreto M.L., Abreu D.L.C., Pereira V.L.A. & Nascimento E.R. [Epidemiological situation of avian mycoplamosis in the State of Rio de Janeiro, Brazil.] Situação epidemiológica da micoplasmose aviária no Estado do Rio de Janeiro. Revista Brasileira de Medicina Veterinária, 37(4):379-385, 2015. Departamento de Saúde Coletiva Veterinária e Saúde Pública, Faculdade de Medicina Veterinária, Universidade Federal Fluminense, Rua Vital Brasil, 64, Santa Rosa, Niterói, RJ 24320-340, Brasil. E-mail: petlela@hotmail.com This study was conducted to characterize the Mycoplasmosis epidemiological situation in Rio de Janeiro State. The state was divided into three regions considered significant in poultry production in the state and 252 samples of tracheal specimens were taken, using swabs dipped in 1.5ml of Frey medium and 2.5 ml of blood from 884 birds, in 47 flocks, from 11broiler chickens farms, 6 laying hens farms and 1 broiler breeders farm, of which were evaluated 334 broilers, 205 laying hens and 345 broilers breeders. The serums were subjected to Rapid Serum Agglutination (RSA) and ELISA for detection of antibodies against Mycoplasma gallisepticum (MG) and M. synoviae (MS) and the swabs used in the polymerase chain reaction (PCR) for those agents detection. Were obtained by SAR for MG and MS in the 884 birds studied, frequencies of 14.25% (126/884) and 13.68% (121/884), respectively. By ELISA, were btained 16.17% (143/884) for MG and 15.61% (138/884) for MS. According to the type of production, it was found in broilers chickens by SAR, 11.37% (38/334) for MG and 9.5% (32/334) for MS and in the ELISA, 9.28% (31/334) for MG and 9.88% (33/334) for MS. In laying chickens, the frequency of positive birds by SAR for MG and MS were in an amount of 42.92% (88/205) and 43.41% (89/205), respectively, and by ELISA, 54.63% (112/205) for MG and 49.75% (102/205) for MS. The broiler breeders shown to be negative for MG by SAR and by ELISA, but for MS, 0.86% (2/345) were positive only by ELISA. When comparing the frequencies for MG and MS in the flocks studied, by SAR, was obtained a total of positivity of 55.31% (26/47) for MG and 63.82% (30/47) for MS, and by ELISA, 51.06% (24/47) and 68.08% (32/47), respectively. For each type of production, was obtained for the broilers chickens a positivity to the SAR of 40% (10/25) for MG e 56% (14/25) for MS; and to the ELISA, 28% (7/25) e 60% (15/25) for MG and MS, respectively. In laying chickens was obtained values of 80% (16/20) for the MG and MS by SAR, whereas by ELISA, was obtained 85% (17/20) for MG and 75% (15/20) for MS. In breeders, by ELISA both two flocks studied were positive only for MS. By PCR, the total of positivity for MG and MS were in an amount of 1.19% (3/252) and 9.92% (25/252), respectively. For each type of production, were obtained 0.78% (1/127) of positive for MS in broilers chickens. In laying hens chickens, the results were 2.6% (3/115) positive for MG and 20.86% (24/115) for MS. About the broiler breeders, there was no positivity by PCR. The frequency of positives for MG and MS, by PCR, in the total of the flocks studied were, respectively, 2.12% (1/47) and 21.27% (10/47). By type of production, were not found positive flocks for MG, however, for MS was obtained 4% (1/25) in the broilers. In laying chickens, it was verified that 5% (1/20) of the flocks were positive for MG and 45% (9/20) for MS. All flocks of broiler breeders were negative for MG and MS. Higher prevalence for MS were detected in the layeing hens farms than in the broilers farms and breeders farm, such as by serology as by PCR. The prevalence of MG was higher in broilers chickens than in broilers breeders. There was high positivity in laying hens, whose data was not considered because included vaccinated chickens. There were differences in the results obtained for the broilers flocks and laying hens flocks and in the positivity among the flocks, which can be attributed to differences in farm building and structures, in the ambience and manger inherent to each type of production. In this research for MG and MS in poultry production, was obtained higher frequency of positive by ELISA than by SAR, with a low agreement between the tests.
Keywords	Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2015-12-01T00:00:00Z
Publisher	Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
Document type	Article ; Online
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Article ; Online: Pré-resfriamento na redução de coliformes em carcaças de frango de corte

Vanessa Silva Simas / Felipe Faccini dos Santos / Raquel Gouvêa / Maria Helena Cosendey de Aquino / Dayse Lima da Costa Abreu / Elmiro Rosendo do Nascimento / Virginia Léo de Almeida Pereira

Ciência Rural, Vol 43, Iss 9, Pp 1618-

2013 Volume 1622

Abstract: O objetivo deste trabalho foi comparar a análise individual (plano de duas classes) e a análise por lotes (plano de três classes), priorizado pelo plano amostragem oficial da ANVISA, na influência do pré-resfriamento de carcaças de frangos de corte na ... ...

Abstract	O objetivo deste trabalho foi comparar a análise individual (plano de duas classes) e a análise por lotes (plano de três classes), priorizado pelo plano amostragem oficial da ANVISA, na influência do pré-resfriamento de carcaças de frangos de corte na redução da contaminação por coliformes termotolerantes. Foram analisadas 240 carcaças de frangos de corte, sendo coletadas 120 amostras antes e 120 após a etapa de pré-resfriamento, para quantificação de coliformes termotolerantes pela técnica de contagem em placas. As médias das contagens obtidas das carcaças coletadas antes e após o pré-resfriamento foram diferentes, com uma redução média de 0,99log10 UFC g-1 de coliformes termotolerantes. Na interpretação dos resultados obtidos antes do pré-resfriamento pela análise individual, 16,7% (20/120) das carcaças foram classificadas como inaceitáveis, enquanto, pela análise por lotes, foram 37,5% (45/120). Houve associação entre a aceitabilidade dos lotes e a passagem pelo chiller com um valor de Odds Ratio de 35,48. Ficou demonstrada a importância da utilização do plano de análise por lotes e da etapa de pré-resfriamento no processo de produção, sendo decisivos para a aceitação dos lotes de carcaças de aves para comercialização pelos parâmetros vigentes na legislação nacional.
Keywords	descontaminação ; controle de qualidade de alimentos ; chiller ; termotolerantes ; plano amostral ; Animal culture ; SF1-1100 ; Agriculture ; S
Language	English
Publishing date	2013-09-01T00:00:00Z
Publisher	Universidade Federal de Santa Maria
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Potentially pathogenic mycoplasmas in the external ear canal of clinically normal cattle in Southeast Brazil

Sandra Batista dos Santos / Elmiro Rosendo do Nascimento / João Luiz Horácio Faccini / Maria Lúcia Barreto / Virginia Léo de Almeida Pereira

Brazilian Journal of Microbiology, Vol 40, Iss 3, Pp 455-

first report Micoplasmas potencialmente patogênicos no canal auditivo de bovinos clinicamente sadios no Sul do Brasil: primeiro relato

2009 Volume 457

Abstract: Mycoplasmas were searched in the ear canal flushing of 60 bovine in Brazil. The prevalence obtained was 80%. The percentages of typified species were 12.5%, for M. alkalenses; 2.1%, M. arginini; 8.35%, M. bovirhinis; 2.1%, M. bovis; 25.0%, M. ... ...

Abstract	Mycoplasmas were searched in the ear canal flushing of 60 bovine in Brazil. The prevalence obtained was 80%. The percentages of typified species were 12.5%, for M. alkalenses; 2.1%, M. arginini; 8.35%, M. bovirhinis; 2.1%, M. bovis; 25.0%, M. conjunctivae; 14.6%, M. mycoides subsp. mycoides LC and 10.4% M. capricolum. Foram pesquisados micoplasmas no conduto auditivo de 60 bovinos no Brasil. A prevalência obtida foi de 80%. A porcentagem das espécies tipificadas foi de M. alkalenses, 12,5%; M. arginini, 2,1%; M. bovirhinis, 8,35%; M. bovis, 2,1%; M. conjunctivae, 25,0%; M. mycoides subsp. mycoides LC, 14,6% e M. capricolum, 10,4%.
Keywords	Mollicutes ; bovinos ; conduto auditivo externo ; immunoperoxidase ; bovine ; external ear canal ; Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
Language	English
Publishing date	2009-09-01T00:00:00Z
Publisher	Sociedade Brasileira de Microbiologia
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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