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  1. Article ; Online: Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242.

    Vivekanandan, Rajesh / Szepes, Monika / Ricci Signorini, Maria Elena / Kravchenko, Denys / Kiefer, Johanna / Berger, Steffen / Fricke, Veronika / Göhring, Gudrun / Gruh, Ina

    Stem cell research

    2022  Volume 66, Page(s) 102981

    Abstract: Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of ... ...

    Abstract Genetically encoded voltage indicators (GEVIs) allow for monitoring membrane potential changes in neurons and cardiomyocytes (CMs) as an alternative to patch-clamp techniques. GEVIs facilitate non-invasive, high throughput screening of electrophysiological properties of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). A dual transgenic hiPSC line with Arclight A242 (GEVI) and an antibiotic resistance cardiac selection cassette was successfully generated from an earlier established hiPSC line MHHi001-A. After cardiac differentiation and selection, purified populations of CMs with constitutive GEVI expression can be utilized for studying cardiac development, disease modeling, and drug testing.
    MeSH term(s) Humans ; Induced Pluripotent Stem Cells/metabolism ; Action Potentials ; Myocytes, Cardiac/metabolism ; Cell Differentiation/physiology ; Electrophysiological Phenomena
    Language English
    Publishing date 2022-11-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2393143-7
    ISSN 1876-7753 ; 1873-5061
    ISSN (online) 1876-7753
    ISSN 1873-5061
    DOI 10.1016/j.scr.2022.102981
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Mitochondrial Dysfunction in Age-Related Metabolic Disorders.

    Natarajan, Venkateswaran / Chawla, Ritu / Mah, Tania / Vivekanandan, Rajesh / Tan, Shu Yi / Sato, Priscila Y / Mallilankaraman, Karthik

    Proteomics

    2020  Volume 20, Issue 5-6, Page(s) e1800404

    Abstract: Aging is a natural biological process in living organisms characterized by receding bioenergetics. Mitochondria are crucial for cellular bioenergetics and thus an important contributor to age-related energetics deterioration. In addition, mitochondria ... ...

    Abstract Aging is a natural biological process in living organisms characterized by receding bioenergetics. Mitochondria are crucial for cellular bioenergetics and thus an important contributor to age-related energetics deterioration. In addition, mitochondria play a major role in calcium signaling, redox homeostasis, and thermogenesis making this organelle a major cellular component that dictates the fate of a cell. To maintain its quantity and quality, mitochondria undergo multiple processes such as fission, fusion, and mitophagy to eliminate or replace damaged mitochondria. While this bioenergetics machinery is properly protected, the functional decline associated with age and age-related metabolic diseases is mostly a result of failure in such protective mechanisms. In addition, metabolic by-products like reactive oxygen species also aid in this destructive pathway. Mitochondrial dysfunction has always been thought to be associated with diseases. Moreover, studies in recent years have pointed out that aging contributes to the decay of mitochondrial health by promoting imbalances in key mitochondrial-regulated pathways. Hence, it is crucial to understand the nexus of mitochondrial dysfunction in age-related diseases. This review focuses on various aspects of basic mitochondrial biology and its status in aging and age-related metabolic diseases.
    MeSH term(s) Aging ; Animals ; Energy Metabolism ; Humans ; Metabolic Diseases/etiology ; Metabolic Diseases/metabolism ; Metabolic Diseases/pathology ; Mitochondria/metabolism ; Mitochondria/pathology ; Reactive Oxygen Species/metabolism ; Signal Transduction
    Chemical Substances Reactive Oxygen Species
    Language English
    Publishing date 2020-03-17
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2032093-0
    ISSN 1615-9861 ; 1615-9853
    ISSN (online) 1615-9861
    ISSN 1615-9853
    DOI 10.1002/pmic.201800404
    Database MEDical Literature Analysis and Retrieval System OnLINE

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