Article ; Online: A spontaneous
microPublication biology
2023 Volume 2023
Abstract: The auxin-inducible degron (AID) system is a widely-used system for conditional protein depletion. During the course of an experiment, we depleted the nuclear hormone receptor transcription factor NHR-23 to study molting, and we recovered a spontaneous ... ...
Abstract | The auxin-inducible degron (AID) system is a widely-used system for conditional protein depletion. During the course of an experiment, we depleted the nuclear hormone receptor transcription factor NHR-23 to study molting, and we recovered a spontaneous suppressor allele that bypassed the L1 larval arrest caused by NHR-23 depletion. These mutants also failed to deplete a BFP::AID reporter in the strain background, suggesting a broader defect in the AID system. These animals carried an in-frame 18 base pair insertion that produced a 6 amino acid repeat in TIR1. The larval arrest in these animals could be restored by expressing a wild-type |
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Language | English |
Publishing date | 2023-10-15 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 2578-9430 |
ISSN (online) | 2578-9430 |
DOI | 10.17912/micropub.biology.000994 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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