Article ; Online: A modeling approach for mean fluorescence intensity value harmonization and cutoff prediction for luminex single antigen bead assays of two different vendors.
2023 Volume 102, Issue 5, Page(s) 557–569
Abstract: Luminex single antigen bead (SAB) kits from One Lambda (OL) and Lifecodes (LC) are widely used for HLA antibody detection but have substantial differences in design and assay protocol resulting in different mean fluorescence intensity (MFI) values. Here, ...
Abstract | Luminex single antigen bead (SAB) kits from One Lambda (OL) and Lifecodes (LC) are widely used for HLA antibody detection but have substantial differences in design and assay protocol resulting in different mean fluorescence intensity (MFI) values. Here, we present a non-linear modeling approach to accurately convert MFI values between two vendors and to establish user-independent MFI cutoffs when analyzing big datasets. HLA antibody data from a total of 47 EDTA-treated sera tested using both OL and LC SAB kits were analyzed. MFI comparisons were made for the common 84 HLA class I and 63 class II beads. In the exploration set (n = 24), a non-linear hyperbola model on raw MFI corrected by locus-specific highest self MFI subtraction yielded the highest correlation (class I r |
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MeSH term(s) | Humans ; HLA Antigens ; Alleles ; Antibodies ; Histocompatibility Testing/methods ; Kidney Transplantation/methods ; Isoantibodies ; Graft Rejection |
Chemical Substances | HLA Antigens ; Antibodies ; Isoantibodies |
Language | English |
Publishing date | 2023-05-02 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2845111-9 |
ISSN | 2059-2310 ; 2059-2302 |
ISSN (online) | 2059-2310 |
ISSN | 2059-2302 |
DOI | 10.1111/tan.15082 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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