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  1. Article: Editorial: Macromolecular interactions in signaling pathways: from classical approaches to virtual reality.

    Leopoldo, Marcello / Contino, Marialessandra / Maudsley, Stuart / Vrecl, Milka

    Frontiers in endocrinology

    2024  Volume 15, Page(s) 1388849

    MeSH term(s) Signal Transduction ; Receptors, G-Protein-Coupled ; Virtual Reality
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2024-02-28
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2024.1388849
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Editorial: Molecular modulators of GPCRs signal transduction.

    Vrecl, Milka / Contino, Marialessandra / Leopoldo, Marcello

    Frontiers in endocrinology

    2023  Volume 14, Page(s) 1252734

    MeSH term(s) Signal Transduction ; Receptors, G-Protein-Coupled
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2023-07-18
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2023.1252734
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, Ca

    Lisec, Barbara / Bozic, Tim / Santek, Iva / Markelc, Bostjan / Vrecl, Milka / Frangez, Robert / Cemazar, Maja

    Cell communication and signaling : CCS

    2024  Volume 22, Issue 1, Page(s) 118

    Abstract: Background: Disruption of Ca: Methods: CaEP was performed on EA.hy926 and HMEC-1 cells with increasing Ca: Results: EA.hy926 cells, at increasing Ca: Conclusions: Our finding show that significant differences in CaEP response and [ ... ...

    Abstract Background: Disruption of Ca
    Methods: CaEP was performed on EA.hy926 and HMEC-1 cells with increasing Ca
    Results: EA.hy926 cells, at increasing Ca
    Conclusions: Our finding show that significant differences in CaEP response and [Ca
    MeSH term(s) Calcium/metabolism ; Acetylcholine/metabolism ; Acetylcholine/pharmacology ; Angiotensin II/pharmacology ; Bradykinin/pharmacology ; Ionomycin/metabolism ; Ionomycin/pharmacology ; Thapsigargin/metabolism ; Cell Line ; Endothelial Cells/metabolism ; Endothelium, Vascular/metabolism ; Gene Expression Profiling ; Electroporation ; Adenosine Triphosphate/metabolism
    Chemical Substances Calcium (SY7Q814VUP) ; Acetylcholine (N9YNS0M02X) ; Angiotensin II (11128-99-7) ; Bradykinin (S8TIM42R2W) ; Ionomycin (56092-81-0) ; Thapsigargin (67526-95-8) ; Adenosine Triphosphate (8L70Q75FXE)
    Language English
    Publishing date 2024-02-12
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2126315-2
    ISSN 1478-811X ; 1478-811X
    ISSN (online) 1478-811X
    ISSN 1478-811X
    DOI 10.1186/s12964-024-01503-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Computational Modeling and Characterization of Peptides Derived from Nanobody Complementary-Determining Region 2 (CDR2) Targeting Active-State Conformation of the β

    Sencanski, Milan / Glisic, Sanja / Kubale, Valentina / Cotman, Marko / Mavri, Janez / Vrecl, Milka

    Biomolecules

    2024  Volume 14, Issue 4

    Abstract: This study assessed the suitability of the complementarity-determining region 2 (CDR2) of the nanobody (Nb) as a template for the derivation of nanobody-derived peptides (NDPs) targeting active-state ... ...

    Abstract This study assessed the suitability of the complementarity-determining region 2 (CDR2) of the nanobody (Nb) as a template for the derivation of nanobody-derived peptides (NDPs) targeting active-state β
    MeSH term(s) Receptors, Adrenergic, beta-2/metabolism ; Receptors, Adrenergic, beta-2/chemistry ; Humans ; Single-Domain Antibodies/chemistry ; Single-Domain Antibodies/pharmacology ; Single-Domain Antibodies/metabolism ; Molecular Dynamics Simulation ; Peptides/chemistry ; Peptides/pharmacology ; Peptides/metabolism ; Complementarity Determining Regions/chemistry ; Molecular Docking Simulation ; Protein Binding ; Amino Acid Sequence ; Protein Conformation ; Cyclic AMP/metabolism
    Chemical Substances Receptors, Adrenergic, beta-2 ; Single-Domain Antibodies ; Peptides ; Complementarity Determining Regions ; Cyclic AMP (E0399OZS9N)
    Language English
    Publishing date 2024-03-30
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom14040423
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Bone tissue morphology of rat offspring lactationally exposed to polychlorinated biphenyl 169 and 155.

    Brankovič, Jana / Jan, Janja / Fazarinc, Gregor / Vrecl, Milka

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 19016

    Abstract: Polychlorinated biphenyls (PCBs) are ubiquitous, persistent, organic pollutants also considered endocrine-disrupting chemicals. Our study examined the effects of lactational exposure to nondioxin-like PCB-155 and/or dioxin-like PCB-169 on longitudinal ... ...

    Abstract Polychlorinated biphenyls (PCBs) are ubiquitous, persistent, organic pollutants also considered endocrine-disrupting chemicals. Our study examined the effects of lactational exposure to nondioxin-like PCB-155 and/or dioxin-like PCB-169 on longitudinal femur growth at the distal epiphyseal growth plate (EGP) in young rats at three different ages [postnatal days (PNDs) 9, 22, and 42]. After delivery, lactating rats were divided into four groups (PCB-169, PCB-155, PCB-155 + 169, and control) and administered PCBs intraperitoneally. The femurs of offspring were used to estimate growth rate (µm/day), and histomorphometric analysis on the distal femur included the thickness of the EGP and zones of proliferation and hypertrophy with calcification. Stereometry was used to determine trabecular bone volume density. In the PCB-169 and PCB-155 + 169 groups, PCB-169 affected longitudinal bone growth in the early postnatal period by interfering with chondrocytes in the EGP zone of proliferation and, to a lesser extent, the zone of hypertrophy. Morphometric alterations in EGP structure diminished until puberty. A slow growth rate persisted in the PCB-169 group until PND 42, while in the PCB-155 group, a fast growth rate between PNDs 9 to 22 was significantly reduced between PNDs 22 to 42. Sterometric assessment showed decreased trabecular bone volume in the PCB-155 + 169 group compared with that in the control on PND 9 and increased in the PCB-169 group compared with that in the PCB-155 group on PND 42. To summarize, studied PCB congeners exerted congener- and age-dependent effects on femur growth rate and its histomorphometric characteristics.
    MeSH term(s) Animals ; Female ; Femur/drug effects ; Femur/growth & development ; Growth Plate/drug effects ; Growth Plate/growth & development ; Lactation ; Male ; Maternal Exposure ; Polychlorinated Biphenyls/toxicity ; Pregnancy ; Prenatal Exposure Delayed Effects ; Rats ; Rats, Wistar
    Chemical Substances Polychlorinated Biphenyls (DFC2HB4I0K) ; 2,4,6,2',4',6'-hexachlorobiphenyl (R9YK3084VS) ; 3,4,5,3',4',5'-hexachlorobiphenyl (T2P1WH546D)
    Language English
    Publishing date 2020-11-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-76057-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Effect of Olive Leaves or Marigold Petal Extract on Oxidative Stress, Gut Fermentative Activity, and Mucosa Morphology in Broiler Chickens Fed a Diet Rich in n-3 Polyunsaturated Fats.

    Pirman, Tatjana / Rezar, Vida / Vrecl, Milka / Salobir, Janez / Levart, Alenka

    The journal of poultry science

    2021  Volume 58, Issue 2, Page(s) 119–130

    Abstract: An experiment in broilers was conducted to investigate the effect of olive ( ...

    Abstract An experiment in broilers was conducted to investigate the effect of olive (
    Language English
    Publishing date 2021-04-29
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2197916-9
    ISSN 1349-0486 ; 1346-7395
    ISSN (online) 1349-0486
    ISSN 1346-7395
    DOI 10.2141/jpsa.0200026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Patterns of human and porcine gammaherpesvirus-encoded BILF1 receptor endocytosis.

    Mavri, Maša / Glišić, Sanja / Senćanski, Milan / Vrecl, Milka / Rosenkilde, Mette M / Spiess, Katja / Kubale, Valentina

    Cellular & molecular biology letters

    2023  Volume 28, Issue 1, Page(s) 14

    Abstract: Background: The viral G-protein-coupled receptor (vGPCR) BILF1 encoded by the Epstein-Barr virus (EBV) is an oncogene and immunoevasin and can downregulate MHC-I molecules at the surface of infected cells. MHC-I downregulation, which presumably occurs ... ...

    Abstract Background: The viral G-protein-coupled receptor (vGPCR) BILF1 encoded by the Epstein-Barr virus (EBV) is an oncogene and immunoevasin and can downregulate MHC-I molecules at the surface of infected cells. MHC-I downregulation, which presumably occurs through co-internalization with EBV-BILF1, is preserved among BILF1 receptors, including the three BILF1 orthologs encoded by porcine lymphotropic herpesviruses (PLHV BILFs). This study aimed to understand the detailed mechanisms of BILF1 receptor constitutive internalization, to explore the translational potential of PLHV BILFs compared with EBV-BILF1.
    Methods: A novel real-time fluorescence resonance energy transfer (FRET)-based internalization assay combined with dominant-negative variants of dynamin-1 (Dyn K44A) and the chemical clathrin inhibitor Pitstop2 in HEK-293A cells was used to study the effect of specific endocytic proteins on BILF1 internalization. Bioluminescence resonance energy transfer (BRET)-saturation analysis was used to study BILF1 receptor interaction with β-arrestin2 and Rab7. In addition, a bioinformatics approach informational spectrum method (ISM) was used to investigate the interaction affinity of BILF1 receptors with β-arrestin2, AP-2, and caveolin-1.
    Results: We identified dynamin-dependent, clathrin-mediated constitutive endocytosis for all BILF1 receptors. The observed interaction affinity between BILF1 receptors and caveolin-1 and the decreased internalization in the presence of a dominant-negative variant of caveolin-1 (Cav S80E) indicated the involvement of caveolin-1 in BILF1 trafficking. Furthermore, after BILF1 internalization from the plasma membrane, both the recycling and degradation pathways are proposed for BILF1 receptors.
    Conclusions: The similarity in the internalization mechanisms observed for EBV-BILF1 and PLHV1-2 BILF1 provide a foundation for further studies exploring a possible translational potential for PLHVs, as proposed previously, and provides new information about receptor trafficking.
    MeSH term(s) Animals ; Humans ; Caveolin 1/metabolism ; Clathrin/metabolism ; Endocytosis ; Epstein-Barr Virus Infections ; Herpesvirus 4, Human/metabolism ; Receptors, G-Protein-Coupled/metabolism ; Swine ; Viral Proteins/metabolism
    Chemical Substances Caveolin 1 ; Clathrin ; Receptors, G-Protein-Coupled ; BILF1 protein, Epstein-Barr virus ; Viral Proteins
    Language English
    Publishing date 2023-02-21
    Publishing country England
    Document type Letter
    ZDB-ID 2108724-6
    ISSN 1689-1392 ; 1689-1392
    ISSN (online) 1689-1392
    ISSN 1689-1392
    DOI 10.1186/s11658-023-00427-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Correction: Patterns of human and porcine gammaherpesvirus-encoded BILF1 receptor endocytosis.

    Mavri, Maša / Glišić, Sanja / Senćanski, Milan / Vrecl, Milka / Rosenkilde, Mette M / Spiess, Katja / Kubale, Valentina

    Cellular & molecular biology letters

    2023  Volume 28, Issue 1, Page(s) 94

    Language English
    Publishing date 2023-11-24
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2108724-6
    ISSN 1689-1392 ; 1689-1392
    ISSN (online) 1689-1392
    ISSN 1689-1392
    DOI 10.1186/s11658-023-00512-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Tff3 Deficiency Differentially Affects the Morphology of Male and Female Intestines in a Long-Term High-Fat-Diet-Fed Mouse Model.

    Šešelja, Kate / Bazina, Iva / Vrecl, Milka / Farger, Jessica / Schicht, Martin / Paulsen, Friedrich / Baus Lončar, Mirela / Pirman, Tatjana

    International journal of molecular sciences

    2023  Volume 24, Issue 22

    Abstract: Trefoil factor family protein 3 (Tff3) protects the gastrointestinal mucosa and has a complex mode of action in different tissues. Here, we aimed to determine the effect of Tff3 deficiency on intestinal tissues in a long-term high-fat-diet (HFD)-fed ... ...

    Abstract Trefoil factor family protein 3 (Tff3) protects the gastrointestinal mucosa and has a complex mode of action in different tissues. Here, we aimed to determine the effect of Tff3 deficiency on intestinal tissues in a long-term high-fat-diet (HFD)-fed model. A novel congenic strain without additional metabolically relevant mutations (
    MeSH term(s) Mice ; Male ; Animals ; Female ; Proteins ; Diet, High-Fat/adverse effects ; Mice, Inbred Strains ; Duodenum ; Mice, Inbred C57BL ; Trefoil Factor-3/genetics
    Chemical Substances Proteins ; Trefoil Factor-3
    Language English
    Publishing date 2023-11-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms242216342
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Male Reproductive Organ Weight: Criteria for Detection of Androstenone-Positive Carcasses in Immunocastrated and Entire Male Pigs

    Fazarinc, Gregor / Batorek-Lukač, Nina / Škrlep, Martin / Poklukar, Klavdija / Van den Broeke, Alice / Kress, Kevin / Labussière, Etienne / Stefanski, Volker / Vrecl, Milka / Čandek-Potokar, Marjeta

    Animals. 2023 June 20, v. 13, no. 12

    2023  

    Abstract: Immunocastration and rearing of entire males (EMs) are sustainable alternatives to surgical castration. However, these animal carcasses have variable risk of boar taint and should be identified at the slaughter line. We aimed to identify a simple and ... ...

    Abstract Immunocastration and rearing of entire males (EMs) are sustainable alternatives to surgical castration. However, these animal carcasses have variable risk of boar taint and should be identified at the slaughter line. We aimed to identify a simple and reliable indicator of androstenone-related boar taint by evaluating pelvic urogenital tract weight as a marker of boar-taint animals at the slaughter line. The pelvic urogenital tract, testes, and accessory sex glands of EMs and immunocastrates (ICs) were collected, dissected, and weighed, before colorimetric measurements of testicular tissue. Additionally, GnRH antibody titers and testosterone, androstenone, and skatole levels were determined. Our results showed that 81.8% of EMs had androstenone levels above the risk threshold (>0.5 µg/g fat; EM/Aʰⁱᵍʰ subgroup), whereas in ICs, the C/Aʰⁱᵍʰ subgroup with androstenone >0.5 µg/g fat accounted for only 4.3%. Androstenone levels correlated negatively with GnRH antibody titers and positively with testosterone levels and reproductive organ weights. Identification of ICs with androstenone levels above the threshold (IC/Aʰⁱᵍʰ subgroup) may be achieved via testes or pelvic urogenital tract weight measurements. However, in EMs, the latter is a more reliable parameter. A principal component analysis based on these variables and hierarchical clustering also distinguished the Aʰⁱᵍʰ from the Aˡᵒʷ subgroup, irrespective of IC/EM. The findings highlight the possible use of pelvic urogenital tract weight along with testes weight as a simple, reliable, and efficient morphometric indicator for identifying androstenone-positive carcasses of different sex categories.
    Keywords animals ; antibodies ; boar taint ; colorimetry ; immunocastration ; males ; morphometry ; principal component analysis ; risk ; skatole ; slaughter ; testes ; testosterone ; tissue weight
    Language English
    Dates of publication 2023-0620
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13122042
    Database NAL-Catalogue (AGRICOLA)

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