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Article ; Online: A network pharmacology-based study on key pharmacological pathways and targets of Qi Fu Yin acting on Alzheimer's disease.

Xiao, Qiu-Yue / Ye, Tian-Yuan / Wang, Xiao-Long / Han, Lu / Wang, Tong-Xing / Qi, Dong-Mei / Cheng, Xiao-Rui / Wang, Sheng-Qi

Experimental gerontology

2021 Volume 149, Page(s) 111336

Abstract: Background: Alzheimer's disease (AD) is a central nervous system (CNS) disease characterized by progressive cognitive dysfunction and memory loss. Qi Fu Yin is mainly used to treat dementia, particularly AD, in the clinic, but its comprehensive ... ...

Abstract	Background: Alzheimer's disease (AD) is a central nervous system (CNS) disease characterized by progressive cognitive dysfunction and memory loss. Qi Fu Yin is mainly used to treat dementia, particularly AD, in the clinic, but its comprehensive mechanisms are not known. Objective: In this research, we aimed to investigate the mechanisms of Qi Fu Yin in AD by network pharmacology and molecular docking. Methods: First, the chemical constituents in Qi Fu Yin were obtained from five databases and classified according to their structure. Targets of chemical constituents and AD-related targets were also collected from the databases. Then, overlapping genes between Qi Fu Yin and AD were identified by intersection analysis. MetaCore was used to gather enrichment information. Combination synergy analysis was performed by Cytoscape. After ligand-receptor docking, the binding affinity was verified by ADP-Glo™ kinase assay and fluorescence resonance energy transfer (FRET) assay. Results: We found 12 classes with 977 components in Qi Fu Yin. A total of 511 compounds and 577 potential target proteins in Qi Fu Yin were found to be related to AD. The pathways of Qi Fu Yin in AD included oxidative stress and immune response. There was the best binding affinity between 11 pairs of genes and compounds. Furthermore, CDK5 was inhibited by nepetin with an IC Conclusion: Qi Fu Yin might alleviate Tau hyperphosphorylation by nepetin, kaempferol, ceanothic acid and 18 beta-glycyrrhetinic acid.
Language	English
Publishing date	2021-03-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390992-x
ISSN	1873-6815 ; 0531-5565
ISSN (online)	1873-6815
ISSN	0531-5565
DOI	10.1016/j.exger.2021.111336
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Article ; Online: Characteristics of the traditional Liu-Wei-Di-Huang prescription reassessed in modern pharmacology.

Cheng, Xiao-Rui / Qi, Chun-Hui / Wang, Tong-Xing / Zhou, Wen-Xia / Zhang, Yong-Xiang

Chinese journal of natural medicines

2019 Volume 17, Issue 2, Page(s) 103–121

Abstract: Liu-Wei-Di-Huang (LW) is a Yin nourishing and kidney tonifying prescription in traditional Chinese medicine with promising pharmacological characteristics that can be further exploited and developed in modern medicine. We provide a comprehensive and ... ...

Abstract	Liu-Wei-Di-Huang (LW) is a Yin nourishing and kidney tonifying prescription in traditional Chinese medicine with promising pharmacological characteristics that can be further exploited and developed in modern medicine. We provide a comprehensive and detailed literature report on the clinical and experimental pharmacology of LW, including its quality control parameters, phytochemistry, pharmacokinetics, and toxicology. Our literature review indicates that the LW prescription possesses a unique combination of pharmacological characteristics that can be safely used for treating very different diseases. Quality control and pharmacokinetic parameters of LW are mostly based on its major bioactive phytochemical constituents. We postulate that modulating or rebalancing the neuroendocrine immunomodulation network in the body is the underlying mechanism of the multiple pharmacological activities displayed by LW.
MeSH term(s)	Animals ; Drugs, Chinese Herbal/chemistry ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Humans ; Kidney/drug effects ; Medicine, Chinese Traditional ; Neuroimmunomodulation/drug effects ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Phytochemicals/therapeutic use ; Quality Control ; Yin Deficiency/drug therapy
Chemical Substances	Drugs, Chinese Herbal ; Liuwei Dihuang Decoction ; Phytochemicals
Language	English
Publishing date	2019-02-22
Publishing country	China
Document type	Journal Article ; Review
ZDB-ID	2192577-X
ISSN	1875-5364 ; 2095-6975 ; 1672-3651
ISSN (online)	1875-5364
ISSN	2095-6975 ; 1672-3651
DOI	10.1016/S1875-5364(19)30013-5
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Article: Characteristics of the traditional Liu-Wei-Di-Huang prescription reassessed in modern pharmacology

CHENG, Xiao-Rui / QI, Chun-Hui / WANG, Tong-Xing / ZHANG, Yong-Xiang / ZHOU, Wen-Xia

Chinese journal of natural medicines. 2019 Feb., v. 17, no. 2

2019

Abstract	Liu-Wei-Di-Huang (LW) is a Yin nourishing and kidney tonifying prescription in traditional Chinese medicine with promising pharmacological characteristics that can be further exploited and developed in modern medicine. We provide a comprehensive and detailed literature report on the clinical and experimental pharmacology of LW, including its quality control parameters, phytochemistry, pharmacokinetics, and toxicology. Our literature review indicates that the LW prescription possesses a unique combination of pharmacological characteristics that can be safely used for treating very different diseases. Quality control and pharmacokinetic parameters of LW are mostly based on its major bioactive phytochemical constituents. We postulate that modulating or rebalancing the neuroendocrine immunomodulation network in the body is the underlying mechanism of the multiple pharmacological activities displayed by LW.
Keywords	chemical constituents of plants ; immunomodulation ; kidneys ; medicinal properties ; Oriental traditional medicine ; pharmacokinetics ; quality control ; toxicology
Language	English
Dates of publication	2019-02
Size	p. 103-121.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2192577-X
ISSN	1875-5364 ; 2095-6975 ; 1672-3651
ISSN (online)	1875-5364
ISSN	2095-6975 ; 1672-3651
DOI	10.1016/S1875-5364(19)30013-5
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Jinlida Granules Reduce Obesity in db/db Mice by Activating Beige Adipocytes.

Zhou, Hong-Ru / Wang, Tong-Xing / Hao, Yuan-Yuan / Hou, Yun-Long / Wei, Cong / Yao, Bing / Wu, Xuan / Huang, Dan / Zhang, Hui / Wu, Yi-Ling

BioMed research international

2022 Volume 2022, Page(s) 4483009

Abstract: Recent studies indicate existence of beige adipocytes in adults. Upon activation, beige adipocytes burn energy for thermogenesis and contribute to regulation of energy balance. In this study, we have analyzed whether Jinlida granules (JLD) could activate ...

Abstract	Recent studies indicate existence of beige adipocytes in adults. Upon activation, beige adipocytes burn energy for thermogenesis and contribute to regulation of energy balance. In this study, we have analyzed whether Jinlida granules (JLD) could activate beige adipocytes. JLD suspended in 0.5% carboxymethyl cellulose (CMC) was gavage fed to db/db mice at a daily dose of 3.8 g/kg. After 10 weeks, body weight, biochemical, and histological analyses were performed. In situ hybridization, immunofluorescence, and western blotting were conducted to test beige adipocyte activation in mice. X9 cells were induced with induction medium and maintenance medium containing 400
MeSH term(s)	Adipocytes, Beige/metabolism ; Animals ; Drugs, Chinese Herbal ; Mice ; Mice, Inbred C57BL ; Mice, Inbred Strains ; MicroRNAs/metabolism ; Molecular Docking Simulation ; Obesity/drug therapy ; Obesity/metabolism ; Uncoupling Protein 1/genetics ; Uncoupling Protein 1/metabolism
Chemical Substances	Drugs, Chinese Herbal ; MicroRNAs ; Uncoupling Protein 1 ; jinlida
Language	English
Publishing date	2022-05-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	2698540-8
ISSN	2314-6141 ; 2314-6133
ISSN (online)	2314-6141
ISSN	2314-6133
DOI	10.1155/2022/4483009
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Article ; Online: Proteome Profiling Identified Amyloid-β Protein Precursor as a Novel Binding Partner and Modulator of VGLUT1.

Zhou, Jin-Wu / Zhao, Man / Rang, Wen-Liang / Zhang, Xiao-Yan / Liu, Zhen-Ming / Zhang, Liang-Ren / Wang, Tong-Xing / Wu, Chu-Tse / Cheng, Xiao-Rui / Zhou, Wen-Xia

Journal of Alzheimer's disease : JAD

2021 Volume 81, Issue 3, Page(s) 981–1038

Abstract: Background: The toxicity of excessive glutamate release has been implicated in various acute and chronic neurodegenerative conditions. Vesicular glutamate transporters (VGLUTs) are the major mediators for the uptake of glutamate into synaptic vesicles. ... ...

Abstract	Background: The toxicity of excessive glutamate release has been implicated in various acute and chronic neurodegenerative conditions. Vesicular glutamate transporters (VGLUTs) are the major mediators for the uptake of glutamate into synaptic vesicles. However, the dynamics and mechanism of this process in glutamatergic neurons are still largely unknown. Objective: This study aimed to investigate the candidate protein partners of VGLUT1 and their regulatory roles in the vesicles in rat brain. Methods: Pull down assay, co-immunoprecipitation assay, or split-ubiquitin membrane yeast two hybrid screening coupled with nanoRPLC-MS/MS were used to identify the candidate protein partners of VGLUT1 in the vesicles in rat brain. The in vitro and in vivo models were used to test effects of AβPP, Atp6ap2, Gja1, and Synataxin on VGLUT1 expression. Results: A total of 255 and 225 proteins and 172 known genes were identified in the pull down assay, co-immunoprecipitation assay, or split-ubiquitin yeast two-hybrid screening respectively. The physiological interactions of SV2A, Syntaxin 12, Gja1, AβPP, and Atp6ap2 to VGLUT1 were further confirmed. Knockdown of Atp6ap2, Gja1, and Synataxin increased VGLUT1 mRNA expression and only knockdown of AβPP increased both mRNA and protein levels of VGLUT1 in PC12 cells. The regulatory function of AβPP on VGLUT1 expression was further confirmed in the in vitro and in vivo models. Conclusion: These results elucidate that the AβPP and VGLUT1 interacts at vesicular level and AβPP plays a role in the regulation of VGLUT1 expression which is essential for maintaining vesicular activities.
MeSH term(s)	Amyloid beta-Protein Precursor/metabolism ; Animals ; Brain/metabolism ; Connexin 43/metabolism ; Glutamic Acid/metabolism ; Neurons/metabolism ; Protein Binding ; Proteome ; Proteomics ; Rats ; Rats, Wistar ; Synapses/metabolism ; Synaptic Vesicles/metabolism ; Syntaxin 1/metabolism ; Vesicular Glutamate Transport Protein 1/metabolism
Chemical Substances	Amyloid beta-Protein Precursor ; Connexin 43 ; Gja1 protein, rat ; Proteome ; Syntaxin 1 ; Vesicular Glutamate Transport Protein 1 ; Glutamic Acid (3KX376GY7L)
Language	English
Publishing date	2021-03-17
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1440127-7
ISSN	1875-8908 ; 1387-2877
ISSN (online)	1875-8908
ISSN	1387-2877
DOI	10.3233/JAD-210117
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Article ; Online: The Anatomy of the Global Football Player Transfer Network: Club Functionalities versus Network Properties.

Liu, Xiao Fan / Liu, Yu-Liang / Lu, Xin-Hang / Wang, Qi-Xuan / Wang, Tong-Xing

PloS one

2016 Volume 11, Issue 6, Page(s) e0156504

Abstract: Professional association football is a game of talent. The success of a professional club hinges largely on its ability of assembling the best team. Building on a dataset of player transfer records among more than 400 clubs in 24 world-wide top class ... ...

Abstract	Professional association football is a game of talent. The success of a professional club hinges largely on its ability of assembling the best team. Building on a dataset of player transfer records among more than 400 clubs in 24 world-wide top class leagues from 2011 to 2015, this study aims to relate a club's success to its activities in the player transfer market from a network perspective. We confirm that modern professional football is indeed a money game, in which larger investment spent on the acquisition of talented players generally yields better team performance. However, further investigation shows that professional football clubs can actually play different strategies in surviving or even excelling this game, and the success of strategies is strongly associated to their network properties in the football player transfer network.
MeSH term(s)	Humans ; Male ; Organizations/economics ; Soccer/economics ; Social Support ; Sports/economics
Language	English
Publishing date	2016-06-02
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0156504
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Article ; Online: Determination of Nine Impurities in Uranium Products by Inductively Coupled Plasma-Mass Spectrometry after UTEVA Extraction Chromatographic Separation

ZHU Liu-chao / WANG Tong-xing / ZHAO Yong-gang / XU Chang-kun / ZHAO Li-fei / JIANG Xiao-yan / ZHAO Xing-hong

Yankuang ceshi, Vol 34, Iss 4, Pp 414-

2015 Volume 419

Abstract: Determination of impurity elements in uranium products is very valuable in nuclear forensics tracing and quality control of fuel cartridges. The accuracy of determination is controlled by procedure blank and the recovery of impurity elements. In this ... ...

Abstract	Determination of impurity elements in uranium products is very valuable in nuclear forensics tracing and quality control of fuel cartridges. The accuracy of determination is controlled by procedure blank and the recovery of impurity elements. In this study, a method for rapid separation of uranium from impurity elements by UTEVA resin and determination of impurity elements by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) was developed. Results show that UTEVA resin has a strong absorption of uranium. When the sample weight is 16.43 mg, decontamination factor for uranium in the whole procedure is greater than 3×105, the recoveries of nine impurity elements range from 95.1% to 105.1%. The contents of impurity elements in national standard material, GBW04205, are consistent with the reference values within the uncertainty (k=2). The proposed method has good decontamination ability for uranium and is suitable for analyzing impurity elements in low weight uranium samples. This method provides technical support for nuclear forensics tracing and quality control of fuel cartridges.
Keywords	uranium products ; impurities ; decontamination factor ; uteva extraction chromatography ; inductively coupled plasma-mass spectrometry ; Geology ; QE1-996.5 ; Ecology ; QH540-549.5
Subject code	616
Language	English
Publishing date	2015-07-01T00:00:00Z
Publisher	Science Press, PR China
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Neuroendocrine immunomodulation network dysfunction in SAMP8 mice and PrP-hAβPPswe/PS1ΔE9 mice: potential mechanism underlying cognitive impairment.

Wang, Jian-Hui / Cheng, Xiao-Rui / Zhang, Xiao-Rui / Wang, Tong-Xing / Xu, Wen-Jian / Li, Fei / Liu, Feng / Cheng, Jun-Ping / Bo, Xiao-Chen / Wang, Sheng-Qi / Zhou, Wen-Xia / Zhang, Yong-Xiang

Oncotarget

2016 Volume 7, Issue 17, Page(s) 22988–23005

Abstract: Senescence-accelerated mouse prone 8 strain (SAMP8) and PrP-hAβPPswe/PS1ΔE9 (APP/PS1) mice are classic animal models of sporadic Alzheimer's disease and familial AD respectively. Our study showed that object recognition memory, spatial learning and ... ...

Abstract	Senescence-accelerated mouse prone 8 strain (SAMP8) and PrP-hAβPPswe/PS1ΔE9 (APP/PS1) mice are classic animal models of sporadic Alzheimer's disease and familial AD respectively. Our study showed that object recognition memory, spatial learning and memory, active and passive avoidance were deteriorated and neuroendocrine immunomodulation (NIM) network was imbalance in SAMP8 and APP/PS1 mice. SAMP8 and APP/PS1 mice had their own specific phenotype of cognition, neuroendocrine, immune and NIM molecular network. The endocrine hormone corticosterone, luteinizing hormone and follicle-stimulating hormone, chemotactic factor monocyte chemotactic protein-1, macrophage inflammatory protein-1β, regulated upon activation normal T cell expressed and secreted factor and eotaxin, pro-inflammatory factor interleukin-23, and the Th1 cell acting as cell immunity accounted for cognitive deficiencies in SAMP8 mice, while adrenocorticotropic hormone and gonadotropin-releasing hormone, colony stimulating factor granulocyte colony stimulating factor, and Th2 cell acting as humoral immunity in APP/PS1 mice. On the pathway level, chemokine signaling and T cell receptor signaling pathway played the key role in cognition impairments of two models, while cytokine-cytokine receptor interaction and natural killer cell mediated cytotoxicity were more important in cognitive deterioration of SAMP8 mice than APP/PS1 mice. This mechanisms of NIM network underlying cognitive impairment is significant for further understanding the pathogenesis of AD and can provide useful information for development of AD therapeutic drug.
Language	English
Publishing date	2016-04-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.8453
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Article ; Online: Low-Voltage, High-Pitch Computerized Tomography Angiography of the Infrarenal Aorta and Lower Extremity Vessels: Assessment of Radiation Dose, Image Quality With Hybrid Iterative Reconstruction, and Efficacy of Test Injection Using a Monitoring Scan at Knee Level.

Zhou, Wen Zhen / Gu, Jian Ping / Yin, Xin Dao / Wang, Li Ping / Lu, Ling Quan / Xu, Hui / Wu, Qian Zhi / Wang, Tong Xing / Zhang, Di / Xu, Quan / Wei, Ying Min

Journal of computer assisted tomography

2016 Volume 40, Issue 3, Page(s) 402–408

Abstract: Objectives: Our aim was to assess image quality and radiation dose of low-voltage high-pitch computed tomography angiography of the infrarenal aorta and lower extremities and evaluate the efficacy of test injection technique using a monitoring scan at ... ...

Abstract	Objectives: Our aim was to assess image quality and radiation dose of low-voltage high-pitch computed tomography angiography of the infrarenal aorta and lower extremities and evaluate the efficacy of test injection technique using a monitoring scan at knee level. Methods: A total of 60 patients with suspected peripheral arterial disease were divided into 2 groups: group 1 (30 patients, 80 kVp, high pitch [3.2], and hybrid iterative reconstruction [sinogram-affirmed iterative reconstruction]) and group 2 (30 patients, 120 kVp, low pitch [1.0], and filtered back projection reconstruction). The test injection technique at knee level was used to determine the scan delay time in group 1. The image quality and radiation exposure were compared. Results: There were significant differences between the 2 groups in mean (SD) arterial attenuation (80 vs 120 kVp: 507.78 [103.01] vs 317.54 [62.03] Hounsfield units, P < 0.001), mean (SD) signal-to-noise ratio (51.04 [20.29] vs 34.66 [9.94], P < 0.001), and contrast-to-noise ratio (44.83 [17.93] vs 28.26 [9.60], P < 0.001). No difference in subjective image quality was found between the 2 groups (all P > 0.05). The imaging time was significantly shorter in group 1 (2.70 [0.11] vs 14.65 [0.90s], P < 0.001). The mean (SD) effective dose was significantly lower in the 80 kVp group (0.76 [0.06] vs 4.29 [0.63] mSv, P < 0.001). Conclusions: The 80-kVp high-pitch computed tomography angiography of the lower limbs using sinogram-affirmed iterative reconstruction yields reduction of radiation exposure as well as obtains acceptable image quality if acquisition protocols are used in conjunction with the test injection technique using monitoring scan at knee level to determine the delay time.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Algorithms ; Aorta, Abdominal/diagnostic imaging ; Aortic Diseases/diagnostic imaging ; Arterial Occlusive Diseases/diagnostic imaging ; Computed Tomography Angiography ; Contrast Media/administration & dosage ; Female ; Humans ; Kidney/blood supply ; Kidney/diagnostic imaging ; Knee/blood supply ; Knee/diagnostic imaging ; Leg/blood supply ; Male ; Middle Aged ; Peripheral Arterial Disease/diagnostic imaging ; Radiation Exposure/analysis ; Radiation Exposure/prevention & control ; Radiation Protection ; Radiographic Image Enhancement ; Radiographic Image Interpretation, Computer-Assisted/methods ; Reproducibility of Results ; Sensitivity and Specificity ; Young Adult
Chemical Substances	Contrast Media
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	80392-3
ISSN	1532-3145 ; 0363-8715
ISSN (online)	1532-3145
ISSN	0363-8715
DOI	10.1097/RCT.0000000000000365
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