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  1. Article ; Online: Microwell fluoride assay screening for enzymatic defluorination.

    Wackett, Lawrence P

    Methods in enzymology

    2024  Volume 696, Page(s) 65–83

    Abstract: There is intense interest in removing fluorinated compounds from the environment, environments are most efficiently remediated by microbial enzymes, and defluorinating enzymes are readily monitored by fluoride determination. Fluorine is the most ... ...

    Abstract There is intense interest in removing fluorinated compounds from the environment, environments are most efficiently remediated by microbial enzymes, and defluorinating enzymes are readily monitored by fluoride determination. Fluorine is the most electronegative element. Consequently, all mechanisms of enzymatic C-F bond cleavage produce fluoride anion, F
    MeSH term(s) Fluorides/analysis ; Fluorides/metabolism ; Drinking Water/analysis ; Halogenation ; Enzyme Assays/methods ; Enzyme Assays/instrumentation
    Chemical Substances Fluorides (Q80VPU408O) ; Drinking Water
    Language English
    Publishing date 2024-01-11
    Publishing country United States
    Document type Journal Article
    ISSN 1557-7988
    ISSN (online) 1557-7988
    DOI 10.1016/bs.mie.2023.12.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Evolutionary obstacles and not C-F bond strength make PFAS persistent.

    Wackett, Lawrence P

    Microbial biotechnology

    2024  Volume 17, Issue 4, Page(s) e14463

    Abstract: The fate of organic matter in the environment, including anthropogenic chemicals, is largely predicated on the enzymatic capabilities of microorganisms. Microbes readily degrade, and thus recycle, most of the ~100,000 commercial chemicals used in modern ... ...

    Abstract The fate of organic matter in the environment, including anthropogenic chemicals, is largely predicated on the enzymatic capabilities of microorganisms. Microbes readily degrade, and thus recycle, most of the ~100,000 commercial chemicals used in modern society. Per- and polyfluorinated compounds (PFAS) are different. Many research papers posit that the general resistance of PFAS to microbial degradation is based in chemistry and that argument relates to the strength of the C-F bond. Here, I advance the opinion that the low biodegradability of PFAS is best formulated as a biological optimization problem, hence evolution. The framing of the problem is important. If it is framed around C-F bond strength, the major effort should focus on finding and engineering new C-F cleaving enzymes. The alternative, and preferred approach suggested here, is to focus on the directed evolution of biological systems containing known C-F cleaving systems. There are now reports of bacteria degrading and/or growing on multiply fluorinated arenes, alkenoic and alkanoic acids. The impediment to more efficient and widespread biodegradation in these systems is biological, not chemical. The rationale for this argument is made in the five sections below that follow the Introduction.
    MeSH term(s) Biodegradation, Environmental ; Bacteria/genetics ; Fluorocarbons
    Chemical Substances Fluorocarbons
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.14463
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Web alert: Fabrication with microbial spores: An annotated selection of World Wide Web sites relevant to the topics in Microbial Biotechnology.

    Wackett, Lawrence P

    Microbial biotechnology

    2023  Volume 16, Issue 10, Page(s) 2007–2008

    MeSH term(s) Biotechnology ; Spores ; Internet
    Language English
    Publishing date 2023-09-27
    Publishing country United States
    Document type News
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.14345
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Microbial global transport: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology reports

    2023  Volume 15, Issue 2, Page(s) 153–154

    MeSH term(s) Environmental Microbiology ; Internet
    Language English
    Publishing date 2023-03-28
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1758-2229
    ISSN (online) 1758-2229
    DOI 10.1111/1758-2229.13150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Marine microbes: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology

    2023  Volume 25, Issue 4, Page(s) 914–915

    MeSH term(s) Environmental Microbiology ; Internet
    Language English
    Publishing date 2023-04-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.16055
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Microbial production of non-canonical amino acids: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology.

    Wackett, Lawrence P

    Microbial biotechnology

    2023  Volume 16, Issue 12, Page(s) 2401–2402

    MeSH term(s) Amino Acids ; Amines
    Chemical Substances Amino Acids ; Amines
    Language English
    Publishing date 2023-10-31
    Publishing country United States
    Document type News
    ZDB-ID 2406063-X
    ISSN 1751-7915 ; 1751-7915
    ISSN (online) 1751-7915
    ISSN 1751-7915
    DOI 10.1111/1751-7915.14376
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Microbes and hydrothermal environments: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology reports

    2023  Volume 15, Issue 1, Page(s) 72–73

    MeSH term(s) Environmental Microbiology ; Internet
    Language English
    Publishing date 2023-02-17
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1758-2229
    ISSN (online) 1758-2229
    DOI 10.1111/1758-2229.13141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Cyanobacterial algal blooms: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology

    2023  Volume 25, Issue 7, Page(s) 1375–1376

    MeSH term(s) Environmental Microbiology ; Cyanobacteria/genetics ; Internet
    Language English
    Publishing date 2023-06-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.16061
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Acid stress in microbes: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology reports

    2023  Volume 15, Issue 4, Page(s) 335–336

    MeSH term(s) Environmental Microbiology ; Internet
    Language English
    Publishing date 2023-06-30
    Publishing country United States
    Document type Journal Article
    ISSN 1758-2229
    ISSN (online) 1758-2229
    DOI 10.1111/1758-2229.13185
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Web Alert: Solvent stress on environmental microbes: An annotated selection of World Wide Web sites relevant to the topics in environmental microbiology.

    Wackett, Lawrence P

    Environmental microbiology

    2023  Volume 25, Issue 8, Page(s) 1563–1564

    MeSH term(s) Environmental Microbiology ; Internet
    Language English
    Publishing date 2023-07-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2020213-1
    ISSN 1462-2920 ; 1462-2912
    ISSN (online) 1462-2920
    ISSN 1462-2912
    DOI 10.1111/1462-2920.16063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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