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Article ; Online: Analysis of biofilm and bacterial communities in the towel environment with daily use.

Kato, Haruro / Okino, Nagisa / Kijitori, Hiroki / Izawa, Yoshifumi / Wada, Yasunao / Maki, Masataka / Yamamoto, Takako / Yano, Takehisa

Scientific reports

2023 Volume 13, Issue 1, Page(s) 7611

Abstract: Towels differ remarkably from other textile products in their fibre structure and usage, and microbial behaviours on towels remain underexplored. Thus, we evaluated biofilm formation on towels during use for 6 months in daily life and analysed its ... ...

Abstract	Towels differ remarkably from other textile products in their fibre structure and usage, and microbial behaviours on towels remain underexplored. Thus, we evaluated biofilm formation on towels during use for 6 months in daily life and analysed its relationship with odour, dullness, and laundry habits. The towels exhibited odour and dullness after 2 months of use and biofilm structures were observed over the 6 months, especially in the ground warp part. Polysaccharides, proteins, nucleic acids, and viable counts on the towels increased over time. The microbiota was significantly different from that on human skin and clothing. Several species of Alphaproteobacteria were correlated with dullness intensity and the quantity of biofilm components. Therefore, bacterial species that specifically adapt to the towel fibre environment could form biofilms. Our results demonstrate bacterial diversity in textile products and suggest careful consideration of the textile fibre material, structure, and usage pattern to control bacterial communities.
MeSH term(s)	Humans ; Bacteria ; Microbiota ; Alphaproteobacteria ; Biofilms
Language	English
Publishing date	2023-05-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-34501-4
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Article ; Online: [Departments of hematology in comparison to the fields of surgery and emergency care].

Wada, Yasunao

Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology

2009 Volume 50, Issue 4, Page(s) 219–223

Abstract: In recent years, there have been reports on increases in non-albicans, and in this study, based on non-albicans isolated in the fields of surgery and emergency care as well as departments of hematology conducting the preventive administration of ... ...

Abstract	In recent years, there have been reports on increases in non-albicans, and in this study, based on non-albicans isolated in the fields of surgery and emergency care as well as departments of hematology conducting the preventive administration of antifungal agents, we investigated the detected bacterial strains, detection rates, and trends in the results for susceptibility to antifungal agents, while focusing on rare Candida spp. According to the results, in the departments of hematology, rare Candida spp. were detected at high rates and the susceptibility was low. IN comparison to the fields of surgery and emergency care, the departments of hematology featured shifts toward rare Candida spp. rather than shifts toward C. glabrata or C. krusei. In the future, it will be necessary to pay attention to trends in the frequency of isolation and the results in regard to the susceptibility of rare Candida spp.
MeSH term(s)	Antifungal Agents/administration & dosage ; Antifungal Agents/pharmacology ; Candida/classification ; Candida/drug effects ; Candida/isolation & purification ; Drug Resistance, Fungal ; Emergency Medical Services ; Hematology ; Hospital Departments ; Humans ; Mycoses/prevention & control ; Premedication ; Surgery Department, Hospital
Chemical Substances	Antifungal Agents
Language	Japanese
Publishing date	2009-11-25
Publishing country	Japan
Document type	Comparative Study ; English Abstract ; Journal Article ; Review
ZDB-ID	1198792-3
ISSN	1882-0476 ; 0916-4804
ISSN (online)	1882-0476
ISSN	0916-4804
DOI	10.3314/jjmm.50.219
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Article ; Online: Epidemiological and molecular characterization of invasive Streptococcus pneumoniae isolated following introduction of 7-valent conjugate vaccine in Kinki region, Japan, 2008-2013.

Toda, Hirofumi / Tanaka, Yuji / Satoh, Kaori / Komatsu, Masaru / Wada, Yasunao / Tobe, Toru / Kamisako, Toshinori

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

2019 Volume 26, Issue 5, Page(s) 451–458

Abstract: Streptococcus pneumoniae is one of the most common bacteria causing community-acquired pneumonia and meningitis. The use of 7-valent pneumococcal conjugate vaccine (PCV7) has reduced the incidence of pneumococcal disease while changing pneumococcal ... ...

Abstract	Streptococcus pneumoniae is one of the most common bacteria causing community-acquired pneumonia and meningitis. The use of 7-valent pneumococcal conjugate vaccine (PCV7) has reduced the incidence of pneumococcal disease while changing pneumococcal population through herd immunity and non-vaccine pneumococci replacement. This study investigated molecular epidemiologic characteristics of pneumococcal strains in the Kinki region of Japan from 2008 to 2013. A total of 159 invasive pneumococcal isolates were characterized by serotyping, antibiotic susceptibility testing, PCR analysis of penicillin-binding protein genes, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). In adult populations, pediatric PCV7 introduction decreased isolates expressing PCV7 serotypes via herd immunity and increased isolates expressing non-PCV7 serotypes. The rate of penicillin resistance and isolates with alterations in all three pbp genes was higher in PCV7 type isolates than in non-PCV7 type isolates. In MLST analysis, all of serotype 19F isolates were of the same sequence type, ST236, which is the antimicrobial-resistant clone Taiwan
MeSH term(s)	Adolescent ; Adult ; Child ; Community-Acquired Infections/epidemiology ; Community-Acquired Infections/genetics ; Community-Acquired Infections/microbiology ; Community-Acquired Infections/prevention & control ; Electrophoresis, Gel, Pulsed-Field ; Heptavalent Pneumococcal Conjugate Vaccine/therapeutic use ; Humans ; Immunologic Factors/therapeutic use ; Japan/epidemiology ; Microbial Sensitivity Tests ; Molecular Epidemiology ; Multilocus Sequence Typing ; Penicillin Resistance ; Penicillin-Binding Proteins/genetics ; Pneumococcal Infections/epidemiology ; Pneumococcal Infections/genetics ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Serogroup ; Serotyping ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/isolation & purification ; Vaccines, Conjugate/therapeutic use
Chemical Substances	Heptavalent Pneumococcal Conjugate Vaccine ; Immunologic Factors ; Penicillin-Binding Proteins ; Vaccines, Conjugate
Language	English
Publishing date	2019-12-20
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1355399-9
ISSN	1437-7780 ; 1341-321X
ISSN (online)	1437-7780
ISSN	1341-321X
DOI	10.1016/j.jiac.2019.11.010
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Article: [Laboratory-based surveillance of antibacterial drug-resistant bacteria in the Kinki region].

Toyokawa, Masahiro / Komatsu, Masaru / Wada, Yasunao

Rinsho byori. The Japanese journal of clinical pathology

2013 Volume 61, Issue 8, Page(s) 715–720

Abstract: The study of bacterial resistance in the Kinki region of Japan (SBRK) was launched in 1997 for the purpose of monitoring antibacterial drug-resistant bacteria. In SBRK, laboratory-based surveillance, such as of Streptococcus pneumonia, Haemophillus ... ...

Abstract	The study of bacterial resistance in the Kinki region of Japan (SBRK) was launched in 1997 for the purpose of monitoring antibacterial drug-resistant bacteria. In SBRK, laboratory-based surveillance, such as of Streptococcus pneumonia, Haemophillus influenzae, extended spectrum beta-lactamase-producing Enterobacteriaceae, carbapenemase producing Gram-negative rods, etc. has been carried out for over 10 years and the results have been reported through seminars and conference presentations. Technical seminars of detection methods for clinically important drug-resistant bacteria were also held in order to contribute to improving the quality of microbiology laboratories. To conduct appropriate infection control of drug-resistant bacteria, effective utilization of quality-assured microbiological data and information sharing of regional epidemiological data is very important. The construction of regional cooperation systems among microbiology laboratories and with public institutions is strongly required.
MeSH term(s)	Drug Resistance, Bacterial ; Humans ; Infection Control/methods ; Information Dissemination ; Japan ; Laboratories, Hospital ; Population Surveillance
Language	Japanese
Publishing date	2013-08
Publishing country	Japan
Document type	English Abstract ; Journal Article
ZDB-ID	604196-6
ISSN	0047-1860 ; 0485-1404
ISSN	0047-1860 ; 0485-1404
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Article ; Online: Vancomycin loading dose is associated with increased early clinical response without attainment of initial target trough concentration at a steady state in patients with methicillin-resistant Staphylococcus aureus infections.

Ueda, Takashi / Takesue, Yoshio / Nakajima, Kazuhiko / Ichiki, Kaoru / Ishikawa, Kaori / Takai, Yoshiko / Yamada, Kumiko / Wada, Yasunao / Tsuchida, Toshie / Otani, Naruhito / Takahashi, Yoshiko / Ishihara, Mika / Shibata, Sumiyo / Ikeuchi, Hiroki / Uchino, Motoi / Kimura, Takeshi

Journal of clinical pharmacy and therapeutics

2020 Volume 45, Issue 4, Page(s) 682–690

Abstract: What is known and objective: Vancomycin therapeutic guidelines suggest a loading dose of 25-30 mg/kg for seriously ill patients. However, high-quality data to guide the use of loading doses are lacking. We aimed to evaluate whether a loading dose (a) ... ...

Abstract	What is known and objective: Vancomycin therapeutic guidelines suggest a loading dose of 25-30 mg/kg for seriously ill patients. However, high-quality data to guide the use of loading doses are lacking. We aimed to evaluate whether a loading dose (a) achieved a target trough concentration at steady state and (b) improved early clinical response. Methods: Patients with an estimated glomerular filtration rate ≥ 90 mL/min/1.73 m Results: There was no significant difference in C What is new and conclusion: A loading dose of 25 mg/kg followed by 15 mg/kg twice daily did not achieve the optimal Cmin at steady state in patients with normal renal function. However, more early clinical responses were obtained with a loading dose compared with traditional dosing, possibly because of a prompt albeit temporary achievement of a more effective concentration.
MeSH term(s)	Aged ; Anti-Bacterial Agents/administration & dosage ; Critical Illness ; Female ; Glomerular Filtration Rate/drug effects ; Humans ; Male ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Middle Aged ; Retrospective Studies ; Staphylococcal Infections/drug therapy ; Vancomycin/administration & dosage
Chemical Substances	Anti-Bacterial Agents ; Vancomycin (6Q205EH1VU)
Language	English
Publishing date	2020-04-17
Publishing country	England
Document type	Journal Article
ZDB-ID	639006-7
ISSN	1365-2710 ; 0269-4727
ISSN (online)	1365-2710
ISSN	0269-4727
DOI	10.1111/jcpt.13144
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Article: [Reference range of the auditory brainstem response for the patients in the NICU].

Hatakeda, Noriko / Kuroda, Miho / Igaki, Ayumi / Fujii, Seiji / Inuzumi, Koji / Wada, Yasunao / Koshiba, Masahiro

Rinsho byori. The Japanese journal of clinical pathology

2011 Volume 59, Issue 2, Page(s) 134–137

Abstract: Since the auditory brainstem response (ABR) test is a test for the brainstem capacity and hearing function, it is recorded at the discharge from the neonatal intensive care unit (NICU) in our hospital. Although the reference range for the normal full- ... ...

Abstract	Since the auditory brainstem response (ABR) test is a test for the brainstem capacity and hearing function, it is recorded at the discharge from the neonatal intensive care unit (NICU) in our hospital. Although the reference range for the normal full-term newborn infants is well documented, that for the pre-term newborn and/or low birth weight infants is yet to be determined. The latency between wave-I peak and wave-V peak (I-V inter peak latency) on the ABR test was measured on the 254 infants (124 males and 130 females) of 36 to 45 corrected weeks old which was defined as the sum of the gestational weeks and weeks after birth at the test. The reference range for each corrected age group is set as mean +/- standard deviation. The mean value tends to be short as the corrected age increases, while it is not affected by the gestational weeks, body weight on birth, blood bilirubin level, neonatal asphyxia (defined as Apgar score at 1 minute after birth is less than 5), or intrauterine growth retardation. Eight out of 254 patients showed the abnormal values; among the 8 patients, 3 had brain diseases (intraventricular hemorrhage, intracranial hemorrhage, and microcephaly) and 1 with Down's syndrome. Thus the brain stem function testing at a discharge from NICU is important for the neurophysiological prognosis of the patients and the ABR test with the reference range defined here can be a useful tool.
MeSH term(s)	Evoked Potentials, Auditory, Brain Stem ; Female ; Humans ; Infant, Low Birth Weight ; Infant, Newborn ; Intensive Care Units, Neonatal ; Male ; Reference Values
Language	Japanese
Publishing date	2011-02
Publishing country	Japan
Document type	English Abstract ; Journal Article
ZDB-ID	604196-6
ISSN	0047-1860 ; 0485-1404
ISSN	0047-1860 ; 0485-1404
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Article ; Online: A novel method for the purification of DNA by capturing nucleic acid and magnesium complexes on non-woven fabric filters under alkaline conditions for the gene diagnosis of tuberculosis by loop-mediated isothermal amplification (LAMP).

Fukasawa, Tadashi / Oda, Naozumi / Wada, Yasunao / Tamaru, Aki / Fukushima, Yukari / Nakajima, Chie / Suzuki, Yasuhiko

Japanese journal of infectious diseases

2010 Volume 63, Issue 4, Page(s) 246–250

Abstract: A novel method for purifying DNA from clinical samples based on the complex formation of DNA and magnesium ion (Mg(2+)) was developed for the detection of Mycobacterium tuberculosis. The formation of a DNA-Mg(2+) complex under alkaline conditions was ... ...

Abstract	A novel method for purifying DNA from clinical samples based on the complex formation of DNA and magnesium ion (Mg(2+)) was developed for the detection of Mycobacterium tuberculosis. The formation of a DNA-Mg(2+) complex under alkaline conditions was observed by analyzing electrophoretic mobility reduction of DNA on agarose gel. The DNA-Mg(2+) complex increases the efficacy of DNA recovery from the sample solution on polyethylene terephthalate non-woven fabric (PNWF) filters. Among the various divalent metal cations, only Mg(2+) was associated with this effect. The applicability of DNA recovered on the PNWF filter was examined for the gene amplification method; loop-mediated isothermal amplification (LAMP). DNA on the PNWF filter could be used for the amplification of specific DNA fragments without elution from the filter. Using this method, DNA was directly purified from M. tuberculosis spiked sputum and examined by LAMP assay, showing a high sensitivity in comparison to the commercially available DNA extraction kit. These results indicated that the method developed in this study is useful for rapid gene diagnosis of tuberculosis.
MeSH term(s)	Bacteriological Techniques/methods ; DNA/isolation & purification ; DNA/metabolism ; Filtration/methods ; Humans ; Magnesium/isolation & purification ; Magnesium/metabolism ; Mycobacterium tuberculosis/genetics ; Nucleic Acid Amplification Techniques/methods ; Tuberculosis/diagnosis
Chemical Substances	DNA (9007-49-2) ; Magnesium (I38ZP9992A)
Language	English
Publishing date	2010-07
Publishing country	Japan
Document type	Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1478383-6
ISSN	1884-2836 ; 1344-6304
ISSN (online)	1884-2836
ISSN	1344-6304
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Article ; Online: Complete Nucleotide Sequence of pKOI-34, an IncL/M Plasmid Carrying blaIMP-34 in Klebsiella oxytoca Isolated in Japan.

Shimada, Norimitsu / Kayama, Shizuo / Shigemoto, Norifumi / Hisatsune, Junzo / Kuwahara, Ryuichi / Nishio, Hisaaki / Yamasaki, Katsutoshi / Wada, Yasunao / Sueda, Taijiro / Ohge, Hiroki / Sugai, Motoyuki

Antimicrobial agents and chemotherapy

2016 Volume 60, Issue 5, Page(s) 3156–3162

Abstract: We determined the complete nucleotide sequence of a self-transmissible IncL/M plasmid, pKOI-34, from a Klebsiella oxytoca isolate. pKOI-34 possessed the core structure of an IncL/M plasmid found in Erwinia amylovora, pEL60, with two mobile elements ... ...

Abstract	We determined the complete nucleotide sequence of a self-transmissible IncL/M plasmid, pKOI-34, from a Klebsiella oxytoca isolate. pKOI-34 possessed the core structure of an IncL/M plasmid found in Erwinia amylovora, pEL60, with two mobile elements inserted, a transposon carrying the arsenic resistance operon and a Tn21-like core module (tnp and mer modules) piggybacking blaIMP-34 as a class 1 integron, In808, where blaIMP-34 confers a resistance to carbapenems in K. oxytoca and Klebsiella pneumoniae.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; DNA Transposable Elements/genetics ; Japan ; Klebsiella oxytoca/drug effects ; Klebsiella oxytoca/genetics ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Plasmids/genetics
Chemical Substances	Anti-Bacterial Agents ; Bacterial Proteins ; DNA Transposable Elements
Language	English
Publishing date	2016-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.02507-15
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Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Antimicrobial susceptibility surveillance of obligate anaerobic bacteria in the Kinki area.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

2019 Volume 25, Issue 11, Page(s) 837–844

Abstract: Obligate anaerobes exist as resident flora in various sites in humans, but they are also emphasized as endogenous causative microorganism of infections. We performed surveillance to understand the trend of drug susceptibility in obligate anaerobic ... ...

Abstract	Obligate anaerobes exist as resident flora in various sites in humans, but they are also emphasized as endogenous causative microorganism of infections. We performed surveillance to understand the trend of drug susceptibility in obligate anaerobic bacteria in the Kinki area of Japan. In the experiment, we used 156 obligate anaerobe isolates collected from 13 institutions that participated in the Study of Bacterial Resistance Kinki Region of Japan. MALDI Biotyper was used to identify the collected strains, and among the 156 test strains, those that could be identified with an accuracy of Score Value 2.0 or more included 6 genera, 30 species, and 144 strains (Bacteroides spp. 77 strains, Parabacteroides sp. 2 strains, Prevotella spp. 29 strains, Fusobacterium spp. 14 strains, Porphyromonas spp. 2 strains, and Clostridioides difficile 20 strains), and they were assigned as subject strains for drug susceptibility testing. The drug susceptibility test was carried out by broth microdilution method using Kyokuto Opt Panel MP ANA (Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan) and judged according to CLSI criteria. As a result, Bacteroides and Parabacteroides species showed good sensitivities to tazobactam-piperacillin, imipenem, metronidazole and chloramphenicol, and low sensitivities to ampicillin, cefoperazone and vancomycin. Prevotella species showed good sensitivities to sulbactam-ampicillin, tazobactam-piperacillin, cefmetazole, imipenem, doripenem and metronidazole. Susceptibility rates to other drugs were slightly different depending on the bacterial species. Both Fusobacterium spp. and Porphyromonas spp. showed high sensitivities to many drugs. C. difficile was highly sensitive to vancomycin and metronidazole, having MIC
MeSH term(s)	Anti-Infective Agents/therapeutic use ; Bacteria, Anaerobic/drug effects ; Bacterial Infections/drug therapy ; Bacterial Infections/microbiology ; Drug Resistance, Bacterial/drug effects ; Humans ; Japan ; Microbial Sensitivity Tests
Chemical Substances	Anti-Infective Agents
Language	English
Publishing date	2019-08-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1355399-9
ISSN	1437-7780 ; 1341-321X
ISSN (online)	1437-7780
ISSN	1341-321X
DOI	10.1016/j.jiac.2019.07.018
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Article: A novel metallo-β-lactamase, IMP-34, in Klebsiella isolates with decreased resistance to imipenem

Shigemoto, Norifumi / Kayama, Shizuo / Kuwahara, Ryuichi / Hisatsune, Junzo / Kato, Fuminori / Nishio, Hisaaki / Yamasaki, Katsutoshi / Wada, Yasunao / Sueda, Taijiro / Ohge, Hiroki / Sugai, Motoyuki

Diagnostic microbiology & infectious disease. 2013 May, v. 76, no. 1

2013

Abstract: We investigated 5 metallo-β-lactamase (MBL)–positive Klebsiella isolates from Japan showing intermediate resistance to imipenem. Sequencing of the MBL gene identified a novel variant of IMP-1 with a single amino acid substitution, Glu87Gly. This variant ... ...

Abstract	We investigated 5 metallo-β-lactamase (MBL)–positive Klebsiella isolates from Japan showing intermediate resistance to imipenem. Sequencing of the MBL gene identified a novel variant of IMP-1 with a single amino acid substitution, Glu87Gly. This variant is designated as IMP-34 where blaIMP₋₃₄ is located on a transmissible plasmid.
Keywords	Klebsiella ; amino acid substitution ; genes ; imipenem ; plasmids ; Japan
Language	English
Dates of publication	2013-05
Size	p. 119-121.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	604920-5
ISSN	0732-8893
ISSN	0732-8893
DOI	10.1016/j.diagmicrobio.2013.02.030
Database	NAL-Catalogue (AGRICOLA)

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