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  1. Article ; Online: Overuse of pharmacological treatments for patients with benign paroxysmal positional vertigo in the emergency department.

    McGillis, Michaela / Roy, Danielle / Savage, David / McIsaac, Sarah / Nicholls, Jenna / Waltenbury, Danielle / Ohle, Robert

    CJEM

    2023  Volume 25, Issue 8, Page(s) 710–711

    Language English
    Publishing date 2023-07-14
    Publishing country England
    Document type Letter
    ISSN 1481-8043
    ISSN (online) 1481-8043
    DOI 10.1007/s43678-023-00549-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The use of RAPD genomic fingerprinting to study relatedness in strains of Acidithiobacillus ferrooxidans.

    Waltenbury, Danielle R / Leduc, L G / Ferroni, G D

    Journal of microbiological methods

    2005  Volume 62, Issue 1, Page(s) 103–112

    Abstract: Twelve strains of Acidithiobacillus ferrooxidans were recovered from acid mine drainage (AMD) sites from three different geographical locations: Copper Cliff, Ontario, Canada; Mineral City, OH, USA; and Cornwall, England. The spread-plate technique and ... ...

    Abstract Twelve strains of Acidithiobacillus ferrooxidans were recovered from acid mine drainage (AMD) sites from three different geographical locations: Copper Cliff, Ontario, Canada; Mineral City, OH, USA; and Cornwall, England. The spread-plate technique and various culture media were used to isolate and purify all strains. DNA was extracted from each purified culture and amplified using PCR and twenty, 10-mer primers. Amplification products were separated by gel electrophoresis and photographed under UV light. The RAPD (Randomly Amplified Polymorphic DNA) profiles were compared on the basis of the presence or absence of each DNA band and a data matrix was constructed. Strain diversity was calculated using the Jaccard's coefficient and UPGMA (Unweighted Pair-Group Arithmetic Average Clustering) cluster analysis. The variations in the banding patterns indicated genomic variability among the twelve A. ferrooxidans strains tested. The primers used in this study grouped the twelve strains into five major groups. Similarities between the strains ranged from 5.49% to 85.14%. These results show that the strains have a high degree of genomic diversity and that the RAPD procedure is a powerful technique to assess strain variability in this bacterium.
    MeSH term(s) Acidithiobacillus/classification ; Acidithiobacillus/genetics ; Cluster Analysis ; DNA Fingerprinting ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Electrophoresis, Agar Gel ; Genetic Variation ; Industrial Waste ; Mining ; Random Amplified Polymorphic DNA Technique
    Chemical Substances DNA, Bacterial ; Industrial Waste
    Language English
    Publishing date 2005-07
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2005.02.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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